UBE1C (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  UBA3
Gene Name:  ubiquitin-like modifier activating enzyme 3
Original Clone ID:
FLH266213.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
Attempted to grow multiple times from plate and tube sources, but consistently unable to grow.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081589
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UNAVAILABLE
No restriction
Special MTA: None
Insert sequence: 1389nts
Open reading frame: 1 to 1389

ATGGCGGATGGCGAGGAGCCGGAGAAGAAAAGAAGGAGAATAGAGGAGCTGCTGGCTGAG
AAAATGGCTGTTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGGAACCAT
GTAAAGAAGTTCCTCGAGCGATCTGGACCCTTCACACACCCTGATTTCGAACCGAGCACT
GAATCTCTCCAGTTCTTGTTAGATACATGTAAAGTTCTAGTCATTGGAGCTGGCGGCTTA
GGATGTGAGCTCCTGAAAAATCTGGCCTTGTCTGGTTTTAGACAGATTCATGTTATAGAT
ATGGACACTATAGATGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATT
GGAAGACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAACTGCAAT
GTAGTTCCACATTTCTACAAGATTCAAGATTTTAACGACACTTTCTATCGACAATTTCAT
ATTATTGTATGTGGACTGGACTCTATCATCGCCAGAAGATGGATAAATGGCATGCTGATA
TCTCTTCTAAATTATGAAGATGGTGTCTTAGATCCAAGCTCCATTGTCCCTTTGATAGAT
GGGGGGACAGAAGGTTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAATGACTGCTTGT
ATCGAATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACCATTGCA
TCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGATGTTGCAGTGGCCTAAG
GAGCAGCCTTTTGGAGAAGGGGTTCCATTAGGTGGAGATGATCCTGAACATATACAATGG
ATTTTCCAAAAATCCCTAGAGAGAGCATCACAATATAATATTAGGGGTGTTACGTATAGG
CTCACTCAAGGGGTAGTAAAAAGAATCATTCCTGCAGTAGCTTCCACAAATGCAGTCATT
GCAGCTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAAGTGCATACATTCCCTTGAAT
AATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACACATTTGAAGCAGAAAGA
AAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTCAAAATATTCAGTTTTCTCCATCAGCT
AAACTACAGGAGGTTTTGGATTATCTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCA
GCCATCACAGCCACCCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCT
ATTGAAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAAGAATTGGGGCTTGTTGAT
GGACAAGAACTGGCGGTTGCTGATGTCACCACCCCACAGACTGTACTATTCAAACTTCAT
TTTACTTCT

Protein sequence predicted based on nucleotide sequence

MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPST
ESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDI
GRPKAEVAAEFLNDRVPNCNVVPHFYKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLI
SLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIA
SMPRLPEHCIEYVRMLQWPKEQPFGEGVPLGGDDPEHIQWIFQKSLERASQYNIRGVTYR
LTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAER
KENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTS
IEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1408
Start on reference sequence 17
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable