USP13 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  USP13
Gene Name:  ubiquitin specific peptidase 13 (isopeptidase T-3)
Original Clone ID:
FLH266406.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00081782
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2589nts
Open reading frame: 1 to 2589

ATGCAGCGCCGGGGCGCCCTGTTCGGCATGCCGGGCGGCAGCGGAGGCAGGAAGATGGCT
GCAGGAGACATCGGCGAGCTGCTAGTGCCCCACATGCCCACGATCCGCGTGCCCAGGTCC
GGCGACAGGGTCTACAAGAACGAGTGCGCCTTCTCCTACGACTCTCCCAATTCTGAAGGT
GGACTCTATGTATGCATGAATACATTTTTGGCCTTTGGAAGGGAACATGTTGAAAGACAT
TTTCGAAAAACTGGACAGAGTGTATACATGCACCTGAAAAGACATGTGCGAGAGAAGGTA
AGAGGGGCGTCTGGTGGAGCGTTACCAAAAAGGAGGAATTCCAAGATTTTTTTAGATCTA
GATACTGATGACGATTTAAATAGCGACGATTATGAATATGAAGATGAAGCCAAACTTGTT
ATATTCCCAGATCACTATGAAATAGCACTACCAAATATTGAGGAGTTACCAGCCCTGGTA
ACAATTGCTTGTGATGCAGTTCTCAGCTCAAAATCTCCATACAGAAAGCAGGACCCAGAC
ACGTGGGAAAATGAATTGCCAGTATCTAAATATGCCAACAACCTCACCCAGCTGGACAAT
GGAGTCAGGATTCCTCCAAGTGGTTGGAAGTGTGCCAGATGCGACCTGCGAGAAAACCTC
TGGTTGAATCTGACTGACGGCTCTGTCCTGTGTGGAAAGTGGTTCTTTGACAGCTCTGGG
GGCAACGGGCATGCGCTGGAGCATTACAGAGACATGGGCTACCCACTAGCCGTGAAACTG
GGAACCATCACTCCTGACGGGGCAGATGTTTATTCTTTTCAAGAAGAAGAACCTGTTTTG
GATCCTCATTTGGCCAAGCACTTAGCGCATTTTGGAATTGATATGCTTCATATGCATGGG
ACAGAGAATGGGCTCCAGGACAATGACATCAAGCTGAGGGTCAGTGAGTGGGAAGTGATC
CAGGAGTCGGGCACGAAACTGAAGCCAATGTATGGTCCTGGCTACACGGGTCTGAAGAAC
CTGGGCAACAGCTGCTATCTCAGCTCTGTCATGCAGGCCATCTTCAGCATCCCAGAATTC
CAGAGAGCGTATGTAGGAAACCTTCCCAGAATATTTGACTACTCGCCTTTAGATCCAACA
CAAGATTTCAACACACAGATGACTAAGTTAGGACATGGCCTTCTCTCAGGCCAGTATTCA
AAGCCTCCGGTGAAATCTGAACTCATTGAACAGGTGATGAAGGAGGAGCACAAGCCACAG
CAGAACGGGATCTCTCCGCGCATGTTTAAGGCCTTTGTAAGCAAGAGCCACCCGGAATTC
TCCTCTAACAGGCAGCAAGATGCCCAGGAATTCTTCTTGCACCTGGTGAATCTAGTAGAG
AGGAACCGCATCGGCTCAGAAAACCCAAGCGATGTTTTTCGTTTTTTGGTGGAAGAACGC
ATTCAGTGCTGTCAGACCCGGAAAGTCCGCTACACGGAGAGGGTGGATTACCTGATGCAG
TTACCTGTGGCCATGGAGGCGGCAACCAACAAGGATGAACTGATCGCTTATGAACTAACG
AGAAGGGAAGCAGAAGCAAACAGAAGACCCCTTCCTGAGTTGGTACGTGCCAAGATACCA
TTTAGTGCCTGCCTTCAGGCCTTCTCTGAACCAGAAAATGTTGATGATTTCTGGAGCAGT
GCCCTACAAGCAAAGTCTGCGGGTGTGAAAACATCTCGCTTTGCTTCATTCCCTGAATAC
TTGGTAGTGCAGATAAAGAAGTTCACTTTTGGTCTTGACTGGGTTCCCAAAAAATTTGAT
GTTTCTATTGATATGCCAGACCTACTTGATATCAACCATCTCCGAGCCAGGGGGTTACAG
CCAGGAGAGGAAGAACTTCCAGACATCAGCCCCCCCATAGTCATTCCTGATGACTCAAAA
GATCGCCTGATGAACCAATTGATAGACCCATCAGACATCGATGAGTCATCAGTGATGCAG
CTGGCCGAGATGGGTTTCCCGCTGGAAGCATGTCGCAAGGCTGTGTACTTCACTGGAAAT
ATGGGCGCCGAGGTGGCCTTCAACTGGATCATTGTTCACATGGAAGAGCCAGATTTTGCT
GAGCCGCTGACCATGCCTGGTTATGGAGGGGCAGCTTCTGCTGGAGCCTCTGTTTTTGGT
GCTTCTGGACTGGATAACCAACCTCCAGAGGAAATCGTAGCTATCATCACCTCCATGGGA
TTTCAGCGAAATCAGGCTATTCAGGCACTACGAGCAACGAATAATAACCTGGAAAGAGCA
CTGGATTGGATCTTTAGCCACCCTGAGTTTGAAGAAGACAGTGATTTTGTGATTGAGATG
GAGAATAATGCCAATGCAAACATTATTTCTGAGGCCAAGCCCGAAGGACCTAGAGTCAAG
GATGGATCTGGAACATATGAGCTATTTGCATTCATCAGTCACATGGGAACATCCACAATG
AGTGGTCATTACATTTGCCATATCAAAAAGGAAGGAAGATGGGTGATTTACAATGACCAC
AAAGTTTGTGCCTCAGAAAGGCCCCCTAAAGACCTGGGCTACATGTACTTTTACCGCAGG
ATACCAAGC

Protein sequence predicted based on nucleotide sequence

MQRRGALFGMPGGSGGRKMAAGDIGELLVPHMPTIRVPRSGDRVYKNECAFSYDSPNSEG
GLYVCMNTFLAFGREHVERHFRKTGQSVYMHLKRHVREKVRGASGGALPKRRNSKIFLDL
DTDDDLNSDDYEYEDEAKLVIFPDHYEIALPNIEELPALVTIACDAVLSSKSPYRKQDPD
TWENELPVSKYANNLTQLDNGVRIPPSGWKCARCDLRENLWLNLTDGSVLCGKWFFDSSG
GNGHALEHYRDMGYPLAVKLGTITPDGADVYSFQEEEPVLDPHLAKHLAHFGIDMLHMHG
TENGLQDNDIKLRVSEWEVIQESGTKLKPMYGPGYTGLKNLGNSCYLSSVMQAIFSIPEF
QRAYVGNLPRIFDYSPLDPTQDFNTQMTKLGHGLLSGQYSKPPVKSELIEQVMKEEHKPQ
QNGISPRMFKAFVSKSHPEFSSNRQQDAQEFFLHLVNLVERNRIGSENPSDVFRFLVEER
IQCCQTRKVRYTERVDYLMQLPVAMEAATNKDELIAYELTRREAEANRRPLPELVRAKIP
FSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFD
VSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQ
LAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFG
ASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEM
ENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDH
KVCASERPPKDLGYMYFYRRIPS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2626
Start on reference sequence 35
Mutations:
None
Discrepancy:
g78a; a1265g,N422S; a1863g
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable