Gene:
Gene Symbol:
ASCC3
Gene Name:
activating signal cointegrator 1 complex subunit 3
Original Clone ID:
FLH266672.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082048
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTTTACCTCGTCTCACAGGAGCCTTGCGTTCCTTTTCAAATGTCACCAAGCAAGAT
AATTATAATGAAGAAGTGGCTGACTTAAAGATAAAGCGATCTAAACTTCATGAACAAGTT
TTAGATTTGGGCCTGACATGGAAGAAGATAATAAAATTTTTGAATGAAAAACTGGAGAAG
AGTAAAATGCAAAGTATAAATGAAGACTTAAAAGATATATTACATGCTGCAAAGCAGATA
GTTGGAACTGATAATGGGAGAGAAGCAATTGAAAGTGGGGCTGCATTTCTCTTCATGACA
TTTCACTTGAAGGACTCTGTTGGTCACAAGGAAACAAAGGCTATCAAACAGATGTTTGGC
CCCTTTCCTTCATCATCTGCCACTGCAGCTTGTAATGCTACTAATCGAATTATTTCTCAT
TTTAGTCAAGATGATTTTACTGCTCTTGTGCAGATGACAGAAAAAGAACATGGCGATAGG
GTTTTTTTTGGTAAAAATTTAGCATTTTCATTTGACATGCATGATTTGGACCACTTTGAC
GAACTGCCAATAAATGGTGAAACTCAGAAAACTATAAGCCTAGATTATAAGAAGTTTCTG
AATGAACATCTCCAGGAGGCTTGCACCCCAGAACTCAAGCCTGTGGAAAAAACAAATGGC
TCCTTTTTGTGGTGTGAAGTTGAAAAGTACCTAAATTCAACTTTGAAGGAAATGACTGAA
GTGCCAAGAGTAGAAGATCTTTGCTGTACTTTATATGATATGCTTGCTTCTATTAAAAGT
GGTGATGAACTTCAGGATGAGCTATTTGAACTGCTGGGACCTGAAGGACTTGAACTTATT
GAGAAACTCCTCCAGAACAGAATTACAATTGTGGATAGATTTCTTAATTCTTCAAATGAT
CATAGGTTTCAGGCTCTTCAAGACAATTGTAAAAAAATTTTAGGAGAAAATGCTAAACCC
AATTATGGTTGTCAAGTCACTATTCAGTCTGAACAAGAAAAGCAGTTAATGAAACAATAT
CGACGTGAAGAAAAAAGAATTGCCAGACGAGAAAAAAAGGCTGGAGAAGATTTAGAAGTT
TCAGAAGGACTTATGTGCTTTGATCCTAAGGAATTGCGGATACAAAGAGAACAGGCACTT
CTGAATGCTAGAAGTGTTCCAATTCTGAGCAGGCAGAGAGATGCAGACGTTGAAAAAATA
CATTATCCCCATGTGTATGATTCCCAGGCTGAAGCCATGAAAACATCAGCATTTATTGCT
GGTGCAAAGATGATTTTGCCAGAAGGAATCCAAAGAGAGAATAACAAGCTTTATGAAGAA
GTAAGGATTTCCTACAGCGAACCAATGCCACTCAGCTTTGAGGAAAAGCCAGTTTATATC
CAAGACTTAGATGAGATCGGACAGCTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATC
CAGTCAATAGTGTTTGAGACTGCCTACAACACCAATGAGAACATGCTGATTTGTGCCCCT
ACAGGAGCTGGAAAAACCAACATTGCAATGCTGACAGTCTTGCATGAAATTCGCCAACAT
TTTCAACAAGGTGTTATCAAAAAGAATGAATTTAAGATTGTATATGTTGCTCCAATGAAA
GCCTTGGCAGCTGAAATGACAGATTACTTCAGCAGACGTCTAGAGCCACTAGGCATCATT
GTGAAAGAATTGACTGGTGACATGCAGTTGTCCAAAAGTGAAATTTTACGAACTCAGATG
CTTGTGACCACACCAGAAAAATGGGATGTAGTGACAAGAAAGAGTGTTGGGGATGTAGCT
CTTTCCCAGATTGTAAGGCTCCTTATTCTTGATGAAGTTCATTTGCTGCATGAAGATAGA
GGACCAGTATTAGAAAGCATAGTTGCCCGTACTTTACGGCAGGTGGAATCCACACAGAGT
ATGATAAGGATTCTCGGACTGTCTGCAACTTTACCTAACTACCTCGATGTTGCCACATTT
TTACATGTTAATCCATACATTGGACTTTTCTTCTTTGATGGCCGTTTTCGACCAGTACCT
CTTGGACAGACATTTTTGGGGATTAAATGTGCAAATAAGATGCAGCAGTTGAATAACATG
GATGAAGTATGTTATGAAAATGTTTTGAAGCAAGTAAAGGCTGGACACCAGGTGATGGTG
TTTGTACATGCTCGAAATGCCACTGTAAGAACAGCTATGTCTCTAATAGAAAGAGCAAAA
AATTGTGGCCATATTCCCTTCTTTTTTTCTACCCAAGGACATGACTATGTACTTGCAGAA
AAACAGGTACAAAGGTCGAGAAATAAGCAAGTACGAGAATTATTCCCAGATGGTTTTAGT
ATTCATCATGCAGGAATGCTTCGGCAGGACAGAAATTTAGTTGAAAACTTGTTTTCTAAT
GGGCATATCAAAGTCCTAGTGTGTACAGCTACGTTAGCCTGGGGTGTCAATCTTCCCGCC
CATGCTGTTATTATTAAGGGAACACAAATATATGCTGCAAAAAGAGGCTCCTTTGTTGAC
CTTGGAATTTTAGATGTCATGCAGATATTTGGTCGAGCTGGACGACCACAATTTGACAAA
TTTGGGGAAGGAATAATTATAACAACGCATGATAAACTCAGCCATTACCTCACTTTGCTC
ACTCAACGAAACCCAATTGAGAGTCAGTTTCTGGAAAGCCTTGCAGATAACCTAAATGCA
GAGATTGCTCTGGGAACAGTTACTAATGTGGAAGAAGCAGTGAAGTGGATAAGTTACACT
TATCTTTATGTACGGATGAGAGCAAATCCATTAGCATATGGCATCAGTCACAAGGCTTAT
CAGATTGACCCAACATTAAGAAAGCATCGAGAACAGTTGGTCATTGAAGTTGGACGAAAA
CTAGACAAAGCTCAGATGATTCGTTTTGAGGAGCGAACTGGATATTTTTCCTCAACTGAT
TTGGGTAGAACTGCCAGCCATTACTATATTAAATACAACACCATTGAGACCTTTAATGAA
CTCTTTGATGCTCACAAAACAGAAGGTGATATCTTTGCCATAGTCTCCAAAGCTGAAGAA
TTTGATCAAATTAAGGTCAGAGAAGAGGAAATAGAGGAGTTAGATACCTTATTAAGCAAT
TTTTGTGAACTCTCCACTCCTGGAGGTGTAGAGAATAGTTATGGGAAAATAAACATCTTA
CTTCAAACTTATATCAGCCGAGGAGAAATGGACAGTTTCTCCCTTATATCAGATTCTGCA
TATGTTGCACAGAATGCAGCTAGAATTGTCCGTGCTCTTTTTGAAATTGCTCTGAGGAAA
CGTTGGCCTACCATGACCTACAGGCTCCTGAATCTTAGTAAAGTCATTGACAAGAGGCTT
TGGGGTTGGGCTAGCCCTTTGAGACAATTTTCAATCCTACCACCACACATCCTAACAAGA
TTAGAAGAAAAAAAGCTTACTGTGGATAAGCTGAAAGACATGAGGAAAGATGAAATAGGT
CACATTTTACATCATGTGAATATTGGACTGAAGGTCAAACAATGTGTTCATCAGATTCCT
TCTGTTATGATGGAAGCATCCATTCAGCCCATCACAAGGACTGTCCTCCGAGTGACACTC
AGCATCTATGCTGATTTCACTTGGAATGATCAGGTACATGGGACAGTAGGAGAACCTTGG
TGGATTTGGGTAGAAGATCCTACAAATGATCATATTTATCATTCAGAGTATTTTCTAGCT
CTAAAAAAACAAGTCATTAGTAAAGAAGCCCAACTACTGGTATTTACAATCCCTATTTTT
GAGCCTTTGCCTTCCCAATACTACATCCGAGCAGTGTCTGATAGATGGTTGGGTGCTGAG
GCAGTATGTATTATCAACTTTCAACATCTAATTCTACCAGAGAGACATCCTCCTCATACA
GAATTACTGGATCTTCAGCCTTTACCAATCACAGCTTTGGGATGTAAAGCATATGAAGCC
CTGTACAACTTCAGCCACTTTAACCCTGTACAGACACAAATATTTCATACATTGTATCAC
ACGGATTGTAATGTCCTACTTGGAGCACCTACTGGATCGGGAAAGACTGTTGCAGCTGAA
TTAGCCATTTTCAGAGTCTTCAACAAATACCCTACTTCAAAGGCGGTATATATTGCACCC
CTAAAAGCCCTAGTACGTGAAAGAATGGATGATTGGAAAGTTAGAATAGAAGAAAAACTT
GGTAAAAAAGTTATTGAACTAACAGGGGATGTGACTCCTGATATGAAATCCATTGCCAAG
GCTGACCTTATCGTCACTACGCCAGAGAAGTGGGATGGAGTCAGCAGAAGCTGGCAAAAT
AGGAACTATGTTCAGCAAGTCACTATTCTCATCATAGATGAGATCCATCTGCTTGGGGAG
GAAAGAGGCCCTGTTCTAGAGGTCATTGTATCTCGAACAAATTTTATCTCATCACACACA
GAAAAGCCTGTTAGAATAGTTGGACTATCTACTGCATTAGCTAATGCCAGAGACCTTGCT
GATTGGCTCAATATTAAGCAGATGGGCTTGTTTAACTTCCGACCATCAGTACGCCCAGTT
CCACTGGAAGTTCACATTCAAGGCTTTCCAGGTCAACATTACTGTCCTCGTATGGCTAGT
ATGAACAAGCCTGCATTTCAGGCAATTAGAAGCCATTCTCCAGCCAAACCTGTTTTGATA
TTTGTCTCATCAAGACGTCAAACTCGTCTTACTGCTTTGGAATTGATCGCCTTCCTGGCT
ACTGAAGAAGATCCAAAGCAGTGGTTAAACATGGATGAAAGAGAGATGGAGAACATCATT
GCAACAGTAAGAGATTCCAACCTCAAGCTGACCCTTGCTTTCGGGATAGGAATGCATCAT
GCTGGACTACATGAGAGGGACCGAAAAACAGTAGAGGAACTATTTGTAAACTGTAAAGTT
CAGGTTCTTATTGCTACAAGCACATTAGCCTGGGGTGTAAACTTTCCAGCTCATTTAGTA
ATTATTAAGGGAACAGAATACTATGATGGAAAAACAAGACGTTATGTGGATTTTCCCATT
ACAGATGTCCTCCAGATGATGGGGCGTGCTGGGAGGCCGCAGTTCGATGACCAAGGCAAA
GCTGTAATTCTAGTTCATGACATAAAGAAAGACTTTTATAAAAAATTTCTTTATGAACCT
TTCCCAGTAGAATCAAGTTTATTAGGAGTGCTCTCTGACCACTTAAATGCAGAGATTGCT
GGTGGTACAATTACATCTAAGCAAGATGCATTGGATTATATCACCTGGACTTACTTTTTC
CGACGTCTTATCATGAATCCCAGCTATTACAATTTGGGTGATGTGAGCCATGATTCTGTG
AACAAGTTTCTGTCCCATCTGATTGAGAAGTCCCTGATTGAATTGGAACTTTCCTACTGT
ATTGAAATTGGAGAGGATAATCGCAGCATTGAACCTCTAACTTATGGCCGAATTGCCTCC
TATTACTATTTGAAGCATCAAACAGTTAAAATGTTCAAGGACCGCTTGAAGCCTGAATGC
AGTACTGAAGAACTGCTTTCAATTCTAAGTGATGCAGAAGAATATACAGATTTGCCAGTG
AGACACAATGAAGATCATATGAATAGTGAACTGGCAAAATGTCTTCCCATTGAATCAAAT
CCTCATTCATTTGACAGCCCTCACACCAAAGCACATCTCCTGCTACAGGCACATCTCAGC
CGAGCCATGCTACCCTGCCCAGATTATGACACTGATACCAAAACAGTCTTGGACCAAGCT
CTCAGAGTATGTCAGGCAATGCTGGACGTGGCTGCAAATCAGGGCTGGCTGGTGACTGTC
CTGAATATCACCAACCTGATTCAGATGGTGATCCAGGGTCGGTGGTTAAAGGACTCTTCT
CTTCTTACACTACCAAACATAGAAAACCATCATCTTCACCTTTTCAAGAAATGGAAGCCG
ATTATGAAGGGCCCACATGCTAGGGGTCGGACCTCCATCGAGTCCCTTCCTGAACTGATC
CATGCCTGTGGAGGGAAAGACCATGTATTTAGCTCCATGGTAGAAAGTGAGCTACATGCT
GCAAAAACGAAACAGGCATGGAATTTCTTATCTCACTTGCCAGTGATAAATGTTGGCATA
AGTGTTAAAGGCTCGTGGGATGACTTAGTTGAAGGACATAATGAACTCTCTGTCTCAACT
CTGACTGCAGACAAACGAGATGACAACAAATGGATCAAATTGCATGCTGACCAAGAGTAT
GTGCTTCAAGTGAGCTTGCAGAGAGTCCACTTTGGGTTCCACAAGGGAAAGCCAGAGAGC
TGTGCAGTTACTCCTCGATTTCCCAAATCAAAAGACGAAGGATGGTTTTTGATATTAGGA
GAAGTGGATAAGAGAGAACTTATTGCTTTGAAAAGAGTAGGATATATTCGAAATCATCAT
GTTGCTTCCCTTTCTTTTTATACCCCTGAAATACCTGGAAGGTATATCTACACATTATAT
TTCATGAGTGACTGCTACCTTGGCCTGGACCAGCAGTATGACATCTATCTCAACGTTACA
CAAGCGAGTCTTTCTGCACAGGTCAACACCAAGGTCTCTGATTCCCTGACTGACCTGGCA
TTAAAG
Protein sequence predicted based on nucleotide sequence
MALPRLTGALRSFSNVTKQDNYNEEVADLKIKRSKLHEQVLDLGLTWKKIIKFLNEKLEK SKMQSINEDLKDILHAAKQIVGTDNGREAIESGAAFLFMTFHLKDSVGHKETKAIKQMFG PFPSSSATAACNATNRIISHFSQDDFTALVQMTEKEHGDRVFFGKNLAFSFDMHDLDHFD ELPINGETQKTISLDYKKFLNEHLQEACTPELKPVEKTNGSFLWCEVEKYLNSTLKEMTE VPRVEDLCCTLYDMLASIKSGDELQDELFELLGPEGLELIEKLLQNRITIVDRFLNSSND HRFQALQDNCKKILGENAKPNYGCQVTIQSEQEKQLMKQYRREEKRIARREKKAGEDLEV SEGLMCFDPKELRIQREQALLNARSVPILSRQRDADVEKIHYPHVYDSQAEAMKTSAFIA GAKMILPEGIQRENNKLYEEVRISYSEPMPLSFEEKPVYIQDLDEIGQLAFKGMKRLNRI QSIVFETAYNTNENMLICAPTGAGKTNIAMLTVLHEIRQHFQQGVIKKNEFKIVYVAPMK ALAAEMTDYFSRRLEPLGIIVKELTGDMQLSKSEILRTQMLVTTPEKWDVVTRKSVGDVA LSQIVRLLILDEVHLLHEDRGPVLESIVARTLRQVESTQSMIRILGLSATLPNYLDVATF LHVNPYIGLFFFDGRFRPVPLGQTFLGIKCANKMQQLNNMDEVCYENVLKQVKAGHQVMV FVHARNATVRTAMSLIERAKNCGHIPFFFSTQGHDYVLAEKQVQRSRNKQVRELFPDGFS IHHAGMLRQDRNLVENLFSNGHIKVLVCTATLAWGVNLPAHAVIIKGTQIYAAKRGSFVD LGILDVMQIFGRAGRPQFDKFGEGIIITTHDKLSHYLTLLTQRNPIESQFLESLADNLNA EIALGTVTNVEEAVKWISYTYLYVRMRANPLAYGISHKAYQIDPTLRKHREQLVIEVGRK LDKAQMIRFEERTGYFSSTDLGRTASHYYIKYNTIETFNELFDAHKTEGDIFAIVSKAEE FDQIKVREEEIEELDTLLSNFCELSTPGGVENSYGKINILLQTYISRGEMDSFSLISDSA YVAQNAARIVRALFEIALRKRWPTMTYRLLNLSKVIDKRLWGWASPLRQFSILPPHILTR LEEKKLTVDKLKDMRKDEIGHILHHVNIGLKVKQCVHQIPSVMMEASIQPITRTVLRVTL SIYADFTWNDQVHGTVGEPWWIWVEDPTNDHIYHSEYFLALKKQVISKEAQLLVFTIPIF EPLPSQYYIRAVSDRWLGAEAVCIINFQHLILPERHPPHTELLDLQPLPITALGCKAYEA LYNFSHFNPVQTQIFHTLYHTDCNVLLGAPTGSGKTVAAELAIFRVFNKYPTSKAVYIAP LKALVRERMDDWKVRIEEKLGKKVIELTGDVTPDMKSIAKADLIVTTPEKWDGVSRSWQN RNYVQQVTILIIDEIHLLGEERGPVLEVIVSRTNFISSHTEKPVRIVGLSTALANARDLA DWLNIKQMGLFNFRPSVRPVPLEVHIQGFPGQHYCPRMASMNKPAFQAIRSHSPAKPVLI FVSSRRQTRLTALELIAFLATEEDPKQWLNMDEREMENIIATVRDSNLKLTLAFGIGMHH AGLHERDRKTVEELFVNCKVQVLIATSTLAWGVNFPAHLVIIKGTEYYDGKTRRYVDFPI TDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKFLYEPFPVESSLLGVLSDHLNAEIA GGTITSKQDALDYITWTYFFRRLIMNPSYYNLGDVSHDSVNKFLSHLIEKSLIELELSYC IEIGEDNRSIEPLTYGRIASYYYLKHQTVKMFKDRLKPECSTEELLSILSDAEEYTDLPV RHNEDHMNSELAKCLPIESNPHSFDSPHTKAHLLLQAHLSRAMLPCPDYDTDTKTVLDQA LRVCQAMLDVAANQGWLVTVLNITNLIQMVIQGRWLKDSSLLTLPNIENHHLHLFKKWKP IMKGPHARGRTSIESLPELIHACGGKDHVFSSMVESELHAAKTKQAWNFLSHLPVINVGI SVKGSWDDLVEGHNELSVSTLTADKRDDNKWIKLHADQEYVLQVSLQRVHFGFHKGKPES CAVTPRFPKSKDEGWFLILGEVDKRELIALKRVGYIRNHHVASLSFYTPEIPGRYIYTLY FMSDCYLGLDQQYDIYLNVTQASLSAQVNTKVSDSLTDLALK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
39
End on reference sequence
6647
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
