Gene:
Gene Symbol:
ZMYM2
Gene Name:
zinc finger, MYM-type 2
Original Clone ID:
FLH266749.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082125
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGACACAAGTTCAGTGGGAGGATTAGAATTGACTGATCAGACTCCTGTTTTATTAGGG
AGTACGGCCATGGCAACTAGTCTCACGAATGTAGGAAACTCATTTAGTGGTCCAGCTAAT
CCTTTAGTGTCTAGATCTAATAAGTTTCAGAACTCGTCAGTGGAAGATGATGATGATGTT
GTTTTTATCGAACCTGTACAACCTCCCCCACCTTCTGTACCAGTGGTAGCTGATCAAAGA
ACCATAACATTTACATCATCAAAAAATGAAGAACTGCAAGGAAATGATTCCAAAATTACT
CCTCCCTCAAAAGAGTTGGCATCTCAGAAGGGAAGTGTAAGTGAGACAATTGTCATTGAT
GATGAAGAGGACATGGAAACAAATCAAGGGCAAGAGAAAAATTCCTCCAATTTTATTGAA
CGAAGACCTCCTGAGACTAAAAACAGAACCAATGATGTGGATTTCTCCACTTCCAGTTTT
TCAAGAAGTAAGGTAAATGCAGGAATGGGTAATAGTGGTATCACCACAGAACCAGACTCT
GAAATTCAGATTGCTAATGTTACAACTTTAGAAACAGGTGTAAGCTCTGTGAATGATGGC
CAATTAGAAAATACTGACGGGCGAGATATGAACTTAATGATTACACATGTAACATCACTG
CAGAATACCAACTTGGGAGATGTCTCTAACGGACTGCAGTCAAGTAATTTTGGTGTTAAT
ATACAAACATACACCCCATCTTTAACTTCACAGACCAAGACTGGAGTAGGACCTTTTAAT
CCTGGTAGAATGAATGTGGCAGGAGACGTTTTTCAGAATGGAGAATCTGCAACTCATCAT
AATCCTGATTCTTGGATCTCCCAGTCAGCTTCATTTCCCCGTAATCAGAAACAACCAGGG
GTGGACTCTTTATCACCAGTGGCCTCACTTCCTAAACAGATTTTCCAGCCGTCTGTCCAA
CAGCAGCCTACTAAACCAGTTAAAGTCACTTGTGCAAACTGCAAAAAACCTTTACAGAAG
GGCCAGACAGCTTATCAACGAAAAGGATCAGCTCACCTCTTTTGTTCTACCACCTGCCTT
TCTTCCTTCTCCCACAAGCCTGCTCCAAAGAAACTCTGTGTTATGTGTAAAAAAGATATA
ACTACAATGAAAGGAACCATTGTTGCTCAAGTGGATTCAAGTGAGTCCTTCCAGGAATTC
TGTAGTACATCTTGTTTATCTCTCTATGAAGACAAACAGAATCCTACTAAAGGAGCTCTA
AATAAATCAAGATGTACAATCTGTGGTAAACTAACTGAGATTCGCCATGAAGTCAGCTTT
AAAAATATGACTCATAAGCTGTGCAGTGACCACTGCTTTAATAGATATAGAATGGCCAAT
GGTTTAATAATGAATTGCTGTGAACAGTGTGGAGAGTACTTGCCCAGTAAAGGTGCTGGA
AATAATGTTCTGGTGATTGATGGTCAACAGAAAAGATTTTGCTGTCAAAGTTGTGTCAGT
GAATACAAACAGGTAGGTAGCCATCCAAGCTTCCTGAAGGAGGTTCGAGATCACATGCAG
GACTCTTTCTTAATGCAGCCTGAGAAATATGGAAAACTGACAACTTGTACTGGTTGCCGA
ACACAGTGCAGGTTTTTTGATATGACTCAGTGTATAGGTCCTAATGGATATATGGAGCCA
TATTGTTCAACTGCTTGTATGAACAGTCACAAGACAAAATATGCAAAATCACAAAGTTTG
GGAATTATTTGCCATTTTTGTAAGCGAAACTCTTTACCTCAATACCAAGCCACAATGCCT
GATGGAAAACTGTACAACTTTTGCAATTCCAGTTGTGTGGCTAAATTTCAGGCTCTAAGT
ATGCAGTCATCTCCAAATGGCCAGTTTGTAGCGCCAAGTGATATTCAGTTGAAATGCAAC
TACTGCAAAAATTCCTTTTGTTCAAAACCAGAAATCCTGGAATGGGAGAACAAAGTGCAT
CAGTTCTGCAGCAAAACTTGTTCAGATGACTATAAGAAGTTGCATTGCATAGTTACATAT
TGCGAATACTGTCAAGAGGAGAAGACTCTTCATGAAACAGTAAATTTCTCTGGCGTTAAG
AGACCTTTCTGTAGTGAAGGCTGCAAATTATTATACAAACAGGATTTTGCCAGACGTTTA
GGATTGAGATGTGTTACTTGCAACTATTGTTCTCAGCTATGTAAGAAGGGAGCAACTAAA
GAACTCGATGGTGTTGTGAGAGATTTCTGCAGTGAAGATTGCTGTAAAAAATTTCAGGAT
TGGTACTACAAGGCTGCAAGGTGTGACTGTTGTAAATCTCAAGGAACTCTTAAAGAGCGA
GTTCAGTGGCGTGGGGAAATGAAACATTTCTGTGATCAACATTGCTTACTGCGTTTCTAC
TGTCAACAAAATGAGCCCAACATGACAACTCAGAAAGGACCTGAAAACTTACATTATGAT
CAGGGTTGTCAGACATCTCGAACCAAAATGACAGGTTCAGCACCACCCCCTTCTCCAACA
CCTAACAAAGAGATGAAGAACAAAGCAGTTCTTTGCAAACCTTTAACAATGACAAAAGCT
ACTTACTGTAAACCTCACATGCAGACCAAATCTTGTCAGACAGATGATACTTGGAGGACA
GAATATGTTCCAGTGCCTATCCCTGTGCCTGTGTATATCCCAGTTCCTATGCACATGTAC
AGTCAGAATATTCCTGTTCCTACTACAGTTCCTGTTCCTGTGCCAGTTCCTGTTTTTCTG
CCTGCTCCATTGGACAGCAGTGAGAAGATTCCTGCAGCAATTGAGGAGCTAAAAAGCAAG
GTTTCTTCAGATGCTCTTGATACAGAGTTGCTTACAATGACGGATATGATGAGTGAAGAC
GAGGGGAAAACAGAGACAACCAACATCAACAGTGTAATTATTGAAACAGATATAATTGGT
TCAGACCTTTTGAAGAACTCTGACCCAGAGACACAGTCCAGCATGCCTGATGTACCATAT
GAACCAGATTTGGATATCGAAATAGATTTTCCCAGAGCTGCTGAGGAGCTTGATATGGAA
AATGAATTTTTATTACCACCTGTTTTTGGCGAAGAATATGAGGAACAGCCCAGACCTCGA
TCTAAAAAAAAGGGAGCCAAGAGAAAGGCTGTATCAGGATACCAGTCTCATGATGATAGT
TCTGACAATTCAGAATGCAGCTTTCCTTTCAAATATACGTATGGCGTAAATGCATGGAAA
CACTGGGTCAAAACTAGGCAACTTGATGAAGATCTTCTGGTATTAGATGAGTTAAAATCT
TCTAAATCAGTAAAGTTAAAAGAGGATCTACTCTCTCACACCACAGCTGAGCTTAACTAT
GGGTTAGCTCATTTTGTCAATGAGATCCGACGGCCAAATGGAGAGAATTATGCACCTGAC
AGCATCTATTACCTTTGCCTTGGAATACAGGAGTATTTGTGTGGAAGTAATCGAAAAGAC
AACATATTTATTGATCCTGGATACCAAACATTTGAGCAAGAATTGAATAAAATACTGCGA
AGCTGGCAACCAAGCATACTTCCAGATGGGTCAATATTCTCTCGAGTTGAAGAAGACTAT
CTCTGGAGGATAAAACAACTAGGATCACACTCTCCAGTAGCTCTTCTGAATACACTGTTC
TACTTTAACACTAAGTATTTTGGCCTGAAAACAGTGGAACAACACTTAAGACTTTCCTTT
GGCACTGTGTTTAGGCATTGGAAAAAAAATCCTTTAACGATGGAAAACAAAGCGTGTCTT
CGATACCAAGTGTCTTCCTTGTGTGGAACAGATAATGAAGATAAAATTACTACTGGAAAA
AGAAAACATGAAGATGATGAGCCAGTATTTGAACAAATTGAAAACACAGCCAATCCTTCC
AGATGTCCTGTGAAAATGTTTGAATGCTACTTGTCTAAAAGTCCACAGAATCTTAATCAG
AGGATGGATGTTTTTTATTTGCAACCAGAATGCTCTAGTTCTACAGATAGCCCTGTCTGG
TATACGTCTACTTCACTGGACCGAAACACCTTGGAAAATATGCTTGTACGGGTTCTTCTA
GTAAAAGATATTTATGATAAAGACAATTATGAACTGGATGAAGACACAGAC
Protein sequence predicted based on nucleotide sequence
MDTSSVGGLELTDQTPVLLGSTAMATSLTNVGNSFSGPANPLVSRSNKFQNSSVEDDDDV VFIEPVQPPPPSVPVVADQRTITFTSSKNEELQGNDSKITPPSKELASQKGSVSETIVID DEEDMETNQGQEKNSSNFIERRPPETKNRTNDVDFSTSSFSRSKVNAGMGNSGITTEPDS EIQIANVTTLETGVSSVNDGQLENTDGRDMNLMITHVTSLQNTNLGDVSNGLQSSNFGVN IQTYTPSLTSQTKTGVGPFNPGRMNVAGDVFQNGESATHHNPDSWISQSASFPRNQKQPG VDSLSPVASLPKQIFQPSVQQQPTKPVKVTCANCKKPLQKGQTAYQRKGSAHLFCSTTCL SSFSHKPAPKKLCVMCKKDITTMKGTIVAQVDSSESFQEFCSTSCLSLYEDKQNPTKGAL NKSRCTICGKLTEIRHEVSFKNMTHKLCSDHCFNRYRMANGLIMNCCEQCGEYLPSKGAG NNVLVIDGQQKRFCCQSCVSEYKQVGSHPSFLKEVRDHMQDSFLMQPEKYGKLTTCTGCR TQCRFFDMTQCIGPNGYMEPYCSTACMNSHKTKYAKSQSLGIICHFCKRNSLPQYQATMP DGKLYNFCNSSCVAKFQALSMQSSPNGQFVAPSDIQLKCNYCKNSFCSKPEILEWENKVH QFCSKTCSDDYKKLHCIVTYCEYCQEEKTLHETVNFSGVKRPFCSEGCKLLYKQDFARRL GLRCVTCNYCSQLCKKGATKELDGVVRDFCSEDCCKKFQDWYYKAARCDCCKSQGTLKER VQWRGEMKHFCDQHCLLRFYCQQNEPNMTTQKGPENLHYDQGCQTSRTKMTGSAPPPSPT PNKEMKNKAVLCKPLTMTKATYCKPHMQTKSCQTDDTWRTEYVPVPIPVPVYIPVPMHMY SQNIPVPTTVPVPVPVPVFLPAPLDSSEKIPAAIEELKSKVSSDALDTELLTMTDMMSED EGKTETTNINSVIIETDIIGSDLLKNSDPETQSSMPDVPYEPDLDIEIDFPRAAEELDME NEFLLPPVFGEEYEEQPRPRSKKKGAKRKAVSGYQSHDDSSDNSECSFPFKYTYGVNAWK HWVKTRQLDEDLLVLDELKSSKSVKLKEDLLSHTTAELNYGLAHFVNEIRRPNGENYAPD SIYYLCLGIQEYLCGSNRKDNIFIDPGYQTFEQELNKILRSWQPSILPDGSIFSRVEEDY LWRIKQLGSHSPVALLNTLFYFNTKYFGLKTVEQHLRLSFGTVFRHWKKNPLTMENKACL RYQVSSLCGTDNEDKITTGKRKHEDDEPVFEQIENTANPSRCPVKMFECYLSKSPQNLNQ RMDVFYLQPECSSSTDSPVWYTSTSLDRNTLENMLVRVLLVKDIYDKDNYELDEDTD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
206
End on reference sequence
4339
Mutations:
None
Discrepancy:
t2672c,V891A; t3440a,L1147H
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
