Gene:
Gene Symbol:
ZRANB3
Gene Name:
zinc finger, RAN-binding domain containing 3
Original Clone ID:
FLH266943.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082319
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCTAGGGTTCATAACATAAAAAAGTCTCTTACACCTCACATTTCTTGTGTGACAAAT
GAATCTGATAATCTGCTGGATTTTTTGCCTGACAGACTAAGAGCAAAGCTACTTCCATTC
CAGAAAGATGGCATCATTTTTGCCCTCAAAAGAAATGGCAGGTGTATGGTGGCTGATGAA
ATGGGTCTAGGAAAGACAATCCAGGCAATTGGAATTACTTACTTCTATAAAGAGGAATGG
CCTCTGTTAATAGTGGTCCCTTCGTCTCTGAGGTACCCTTGGACAGAAGAAATTGAAAAA
TGGATCCCAGAGCTAAGTCCAGAAGAAATCAATGTTATTCAGAATAAAACTGATGTTAGG
AGAATGTCGACCAGTAAAGTGACAGTTCTGGGTTATGGTCTCTTAACCGCAGATGCAAAG
ACTTTGATAGATGCACTGAATAATCAGAACTTCAAAGTAGTTATAGTGGATGAATCACAC
TACATGAAATCCAGAAATGCAACTCGCAGCAGGATTTTATTGCCAATAGTACAGAAAGCC
AGACGAGCCATTCTTCTTACAGGAACACCAGCTTTAGGAAGGCCTGAAGAGCTTTTTATG
CAGATTGAAGCTCTCTTTCCACAAAAATTTGGAAGATGGACCGACTATGCAAAAAGATAC
TGTAATGCACACATCAGATATTTTGGTAAAAGACCTCAGTGGGATTGTAGAGGGGCATCA
AATCTTAATGAACTTCACCAGCTATTAAGTGACATAATGATTAGAAGATTAAAGACTGAA
GTTTTAACCCAGCTACCCCCTAAAGTCAGACAGCGTATTCCATTTGATCTTCCATCAGCA
GCTGCCAAGGAATTGAATACCAGCTTTGAAGAGTGGGAAAAAATAATGAGAACTCCAAAT
TCAGGTGCCATGGAGACAGTCATGGGGTTGATAACTCGCATGTTTAAACAAACTGCTATT
GCCAAGGCAGGTGCTGTAAAGGATTATATTAAGATGATGCTTCAGAATGATTCGCTTAAA
TTTCTGGTTTTTGCTCACCATTTAAGCATGCTCCAAGCTTGCACAGAAGCAGTCATCGAA
AATAAGACTCGTTACATTAGGATAGATGGAAGTGTTTCATCTTCAGAAAGAATACATCTG
GTTAATCAGTTTCAAAAGGATCCTGACACTCGCGTGGCTATCCTAAGCATTCAGGCTGCT
GGCCAGGGATTAACATTTACTGCAGCAAGTCATGTTGTATTTGCTGAGTTGTACTGGGAC
CCTGGACATATAAAACAAGCAGAAGACCGAGCTCACAGAATTGGCCAGTGCAGTTCTGTG
AATATTCACTACCTTATTGCAAATGGAACCCTAGACACCCTTATGTGGGGAATGTTGAAT
CGCAAGGCTCAAGTTACAGGGAGCACACTGAACGGTAGGAAAGAAAAAATTCAGGCTGAG
GAAGGTGATAAGGAAAAATGGGATTTCCTGCAGTTTGCTGAAGCTTGGACTCCAAATGAC
AGTTCTGAAGAGTTAAGGAAGGAAGCTTTGTTCACTCACTTCGAAAAAGAAAAACAGCAT
GATATTCGATCATTTTTTGTACCACAACCTAAAAAAAGACAGTTGATGACCTCCTGTGAC
GAATCAAAGAGATTCCGGGAGGAAAATACTGTAGTGTCATCAGACCCTACAAAAACAGCT
GCAAGAGATATCATCGATTATGAAAGTGATGTTGAACCTGAAACGAAAAGATTGAAATTG
GCTGCCTCGGAAGACCACTGCAGTCCGTCGGAAGAGACACCATCCCAGTCCAAGCAAATC
CGAACTCCACTCGTGGAAAGTGTACAGGAGGCCAAGGCCCAGTTAACCACCCCAGCCTTT
CCTGTAGAGGGCTGGCAATGTAGTCTCTGCACCTATATCAATAATTCAGAGTTACCTTAT
TGTGAAATGTGTGAGACTCCTCAAGGCAGTGCTGTTATGCAAATAGATAGCCTCAACCAT
ATCCAGGATAAAAACGAGAAGGATGATTCTCAGAAAGACACCTCCAAAAAGGTTCAAACT
ATCTCAGACTGTGAAAAACAAGCCCTTGCACAGTCAGAACCTGGCCAGTTGGCTGACAGC
AAGGAAGAAACACCAAAAATTGAGAAAGAAGACGGACTTACATCCCAGCCAGGTAATGAA
CAGTGGAAGAGTTCAGACACTTTGCCAGTGTATGACACCTTAATGTTCTGTGCAAGTAGG
AATACTGACCGGATTCACATCTATACTAAGGATGGAAAACAGATGAGCTGTAATTTCATT
CCTCTGGATATAAAATTAGACCTTTGGGAAGATTTACCAGCAAGCTTTCAGCTGAAACAA
TATCGCTCACTGATTTTGAGATTTGTTCGAGAATGGAGTAGTCTAACTGCCATGAAGCAA
AGGATAATCAGGAAAAGTGGACAGCTATTCTGTAGCCCAATTCTTGCTTTGGAAGAGATC
ACAAAGCAACAAACCAAACAAAATTGCACCAAAAGATACATAACCAAAGAAGATGTTGCC
GTAGCCTCAATGGACAAAGTGAAGAATGTTGGGGGCCATGTCCGTCTGATCACAAAGGAG
TCCAGGCCACGGGATCCTTTCACAAAAAAACTTCTTGAAGATGGAGCCTGTGTCCCATTT
CTAAATCCATACACAGTCCAGGCAGATCTCACTGTGAAGCCCTCTACATCCAAAGGCTAT
TTGCAAGCTGTGGATAATGAAGGAAATCCACTTTGCCTTCGCTGTCAGCAACCCACTTGC
CAGACTAAGCAAGCATGTAAAGCGAACTCTTGGGATTCACGGTTTTGCTCTCTGAAATGT
CAGGAAGAGTTTTGGATTCGATCTAATAACAGTTACCTGAGAGCCAAAGTATTTGAAACT
GAACATGGTGTGTGTCAGCTCTGTAATGTGAACGCACAAGAACTCTTTTTACGTCTGAGA
GATGCCCCTAAAAGTCAGAGGAAGAATCTTCTGTATGCTACCTGGACTTCAAAGCTCCCA
TTAGAACAGCTAAATGAAATGATAAGAAACCCAGGGGAAGGACATTTCTGGCAGGTGGAT
CACATCAAGCCAGTGTATGGGGGAGGAGGACAGTGTTCCCTGGACAACCTGCAGACTCTC
TGCACAGTCTGTCACAAAGAGAGAACTGCCAGACAAGCTAAGGAAAGAAGCCAGGTGAGA
AGACAATCTCTAGCATCAAAGCATGGATCAGACATCACACGATTTTTGGTAAAGAAG
Protein sequence predicted based on nucleotide sequence
MPRVHNIKKSLTPHISCVTNESDNLLDFLPDRLRAKLLPFQKDGIIFALKRNGRCMVADE MGLGKTIQAIGITYFYKEEWPLLIVVPSSLRYPWTEEIEKWIPELSPEEINVIQNKTDVR RMSTSKVTVLGYGLLTADAKTLIDALNNQNFKVVIVDESHYMKSRNATRSRILLPIVQKA RRAILLTGTPALGRPEELFMQIEALFPQKFGRWTDYAKRYCNAHIRYFGKRPQWDCRGAS NLNELHQLLSDIMIRRLKTEVLTQLPPKVRQRIPFDLPSAAAKELNTSFEEWEKIMRTPN SGAMETVMGLITRMFKQTAIAKAGAVKDYIKMMLQNDSLKFLVFAHHLSMLQACTEAVIE NKTRYIRIDGSVSSSERIHLVNQFQKDPDTRVAILSIQAAGQGLTFTAASHVVFAELYWD PGHIKQAEDRAHRIGQCSSVNIHYLIANGTLDTLMWGMLNRKAQVTGSTLNGRKEKIQAE EGDKEKWDFLQFAEAWTPNDSSEELRKEALFTHFEKEKQHDIRSFFVPQPKKRQLMTSCD ESKRFREENTVVSSDPTKTAARDIIDYESDVEPETKRLKLAASEDHCSPSEETPSQSKQI RTPLVESVQEAKAQLTTPAFPVEGWQCSLCTYINNSELPYCEMCETPQGSAVMQIDSLNH IQDKNEKDDSQKDTSKKVQTISDCEKQALAQSEPGQLADSKEETPKIEKEDGLTSQPGNE QWKSSDTLPVYDTLMFCASRNTDRIHIYTKDGKQMSCNFIPLDIKLDLWEDLPASFQLKQ YRSLILRFVREWSSLTAMKQRIIRKSGQLFCSPILALEEITKQQTKQNCTKRYITKEDVA VASMDKVKNVGGHVRLITKESRPRDPFTKKLLEDGACVPFLNPYTVQADLTVKPSTSKGY LQAVDNEGNPLCLRCQQPTCQTKQACKANSWDSRFCSLKCQEEFWIRSNNSYLRAKVFET EHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNEMIRNPGEGHFWQVD HIKPVYGGGGQCSLDNLQTLCTVCHKERTARQAKERSQVRRQSLASKHGSDITRFLVKK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
21
End on reference sequence
3251
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
