FLJ23420 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  LRRC8E
Gene Name:  leucine rich repeat containing 8 family, member E
Original Clone ID:
FLH267113.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082489
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2388nts
Open reading frame: 1 to 2388

ATGATCCCAGTGGCCGAGTTCAAGCAGTTCACGGAACAGCAGCCTGCGTTCAAGGTGCTC
AAACCCTGGTGGGACGTGCTGGCCGAGTACCCCACCGTGGCCATGCTCATGATTGGGGTC
TTTGGCTGCACCCTCCAGGTGACACAGGACAAGATCATCTGTCTACCCAATCATGAGCTC
CAGGAGAACTTATCAGAGGCCCCGTGCCAGCAATTGCTGCCTCGGGGGATCCCTGAGCAG
ATTGGGGCCCTGCAGGAGGTTAAAGGCCTTAAGAACAATTTGGACCTGCAGCAATACAGC
TTTATTAACCAGCTGTGTTATGAGACGGCCCTGCACTGGTATGCCAAGTACTTCCCTTAC
CTCGTGGTCATTCACACACTCATCTTCATGGTCTGCACCAGTTTCTGGTTCAAGTTCCCT
GGCACCAGCTCCAAGATTGAACACTTCATCTCCATCCTGGGCAAGTGTTTCGACTCTCCA
TGGACCACCAGGGCCCTATCCGAGGTCTCCGGGGAGAACCAGAAGGGCCCAGCAGCCACC
GAACGGGCTGCGGCCACCATAGTGGCCACGGCAGGGACCGGGCCGGGGAAGGCAGGGGAG
GGTGAGAAGGAGAAAGTGCTGGCGGAACCGGAGAAGGTGGTGACCGAGCCTCCAGTTGTC
ACCCTGTTGGACAAGAAGGAGGGTGAGCAAGCCAAAGCCCTGTTTGAGAAGGTGAAGAAG
TTCCGCATGCGCGTGGAAGAGGGCGACATCCTGTACACCATGTACATCCGACAGACGGTG
CTGAAAGTGTGTAAGTTCCTGGCCATCCTGGTCTACAACCTGGTCTATGTGGAGAAGATC
AGTTTCCTGGTGGCCTGTAGGGTGGAGACGTCAGAGGTCACGGGCTACGCCAGCTTCTGC
TGCAACCACACCAAGGCCCACCTCTTCTCCAAGCTGGCCTTCTGTTACATCTCCTTTGTG
TGCATCTACGGACTTACCTGCATCTACACGCTCTACTGGCTCTTCCACCGGCCCCTCAAG
GAGTACTCCTTCCGTTCCGTGCGGGAGGAGACTGGCATGGGGGACATTCCTGACGTCAAG
AATGACTTCGCCTTCATGCTGCACCTCATCGATCAGTACGACTCCCTCTACTCCAAGCGC
TTCGCCGTCTTCCTGTCCGAGGTCAGCGAAAGCCGTCTAAAGCAGCTCAATCTCAACCAC
GAGTGGACGCCCGAGAAGCTTCGACAGAAGCTGCAGCGCAATGCCGCGGGCCGGCTGGAG
CTGGCCCTCTGCATGCTGCCGGGTCTGCCCGACACCGTCTTTGAGCTCAGTGAGGTGGAG
TCACTCAGGCTGGAGGCCATCTGCGATATCACCTTCCCCCCGGGGCTGTCACAGCTGGTG
CACTTGCAGGAGCTCAGCTTGCTCCACTCGCCCGCCAGGCTACCCTTCTCCTTGCAGGTC
TTCCTGCGGGACCACCTGAAGGTGATGCGCGTCAAATGCGAGGAGCTCCGCGAGGTGCCG
CTTTGGGTGTTTGGGCTGCGGGGCTTGGAGGAGCTGCACCTGGAGGGGCTTTTCCCCCAG
GAGCTAGCTCGGGCAGCCACCCTGGAGAGCCTCCGGGAGCTGAAGCAGCTCAAGGTGTTG
TCCCTCCGGAGCAACGCCGGGAAGGTGCCAGCCAGTGTGACCGACGTTGCTGGCCACCTG
CAGAGGCTCAGCCTGCACAACGATGGGGCCCGTCTGGTTGCCCTGAACAGCCTCAAGAAG
CTGGCGGCATTGCGGGAGCTGGAGCTGGTGGCCTGCGGGCTGGAGCGCATCCCCCATGCA
GTGTTCAGCCTGGGTGCGCTGCAGGAACTTGACCTCAAGGACAACCACCTGCGCTCCATC
GAGGAAATCCTCAGCTTCCAGCACTGCCGGAAGCTGGTCACGCTCAGGCTGTGGCACAAC
CAGATCGCCTACGTCCCTGAGCACGTGCGGAAGCTCAGGAGCCTGGAGCAGCTCTACCCC
AGCTACAACAAGCTGGAGACCCTGCCCTCCCAGCTCGGCCTGTGCTCAGGCCTCCGTCTG
CTGGATGTGTCCCACAATGGGCTACACTCCCTGCCACCCGAGGTGGGCCTCCTGCAGAAC
CTACAGCACCTGGCCCTCTCCTACAATGCCCTGGAGGCCCTGCCCGAAGAGCTCTTCTTC
TGCCGCAAGCTGCGGACGTTGCTTCTGGGCGACAACCAGCTGAGCCAGCTCTCGCCCCAC
GTGGGTGCCCTCAGAGCCCTCAGCCGCCTGGAGCTCAAAGGCAACCGCTTAGAGGCGCTG
CCAGAAGAACTTGGCAACTGTGGGGGGCTCAAGAAGGCGGGGCTCCTGGTGGAAGACACG
CTTTACCAGGGTCTGCCGGCAGAAGTGCGGGACAAGATGGAGGAGGAA

Protein sequence predicted based on nucleotide sequence

MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYPTVAMLMIGVFGCTLQVTQDKIICLPNHEL
QENLSEAPCQQLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYAKYFPY
LVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAAT
ERAAATIVATAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKK
FRMRVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFC
CNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVK
NDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLE
LALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQV
FLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVL
SLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHA
VFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYP
SYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFF
CRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDT
LYQGLPAEVRDKMEEE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 102
End on reference sequence 2492
Mutations:
None
Discrepancy:
t92c,L31P; t569c,M190T;
 a731g,H244R; t1979c,L660P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable