SPATA5 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SPATA5
Gene Name:  spermatogenesis associated 5
Original Clone ID:
FLH267117.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082493
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2679nts
Open reading frame: 1 to 2679

ATGTCTTCCAAGAAGAATAGAAAGCGGTTGAACCAAAGCGCGGAAAATGGTTCGTCCTTG
CCCTCTGCTGCTTCCTCTTGTGCGGAGGCACGGGCTCCTTCTGCTGGATCAGACTTCGCG
GCAACCTCCGGGACTCTGACGGTGACCAACTTATTAGAAAAGGTAGATGACAAAATTCCT
AAAACATTCCAGAATCCCCTTATTCATCTTGGACTCAACACTATGAAGTCTGCAAATATA
TGTATAGGTCGACCAGTGTTGCTTACTAGTTTGAACGGAAAGCAAGAGGTGTATACAGCC
TGGCCTATGGCAGGATTTCCTGGAGGCAAGGTCGGCCTGAGTGAAATGGCACAGAAAAAT
GTGGGTGTGAGGCCTGGTGATGCCATCCAGGTCCAGCCTCTTGTGGGTGCTGTGCTACAG
GCTGAGGAAATGGATGTGGCACTGAGTGACAAAGATATGGAAATTAATGAAGAAGAACTG
ACTGGTTGTATCCTGAGAAAACTAGATGGCAAGATTGTTTTACCAGGCAACTTTCTGTAT
TGTACATTCTATGGACGACCGTACAAGCTGCAAGTATTGCGAGTGAAAGGGGCAGATGGC
ATGATATTGGGAGGGCCTCAGAGTGACCCTGACACTGATGCCCAAAGAATGGCCTTTGAA
CAGTCCAGCATGGAAACCAGTAGCCTGGAGTTATCCTTACAGCTAAGCCAGTTAGATCTG
GAGGATACCCAGATCCCAACATCAAGAAGTACTCCTTATAAACCAATTGATGACAGAATT
ACAAATAAAGCCAGTGATGTTTTGCTGGATGTTACACAGAGCCCTGGAGATGGCAGTGGA
CTTATGCTAGAGGAAGTCACAGGTCTTAAATGTAATTTTGAATCTGCCAGAGAAGGAAAT
GAGCAACTTACTGAAGAAGAGAGACTGCTAAAGTTCAGCATAGGAGCAAAGTGCAATACT
GATACTTTTTATTTTATTTCTTCAACAACAAGAGTCAATTTTACAGAGATTGATAAAAAT
TCAAAAGAGCAAGACAACCAATTCAAAGTAACTTATGACATGATAGGAGGATTAAGTAGC
CAGCTGAAAGCAATTAGAGAAATAATTGAATTGCCCCTCAAACAGCCTGAGCTTTTCAAG
AGTTATGGAATTCCTGCCCCTAGAGGAGTGTTACTTTATGGTCCTCCAGGTACTGGAAAA
ACAATGATCGCCAGGGCTGTTGCTAATGAAGTTGGAGCCTATGTTTCTGTAATTAATGGT
CCTGAAATTATAAGCAAATTCTATGGTGAGACTGAAGCAAAGTTACGTCAGATATTTGCT
GAAGCCACTCTACGACACCCATCAATTATTTTTATTGATGAGCTGGATGCACTTTGTCCG
AAAAGAGAGGGGGCCCAGAATGAAGTGGAAAAAAGAGTTGTGGCTTCACTCTTAACACTG
ATGGATGGCATTGGTTCAGAAGTAAGTGAAGGACAAGTGTTGGTTCTTGGGGCCACAAAT
CGCCCTCATGCCTTGGATGCTGCTCTCCGAAGACCTGGGCGATTTGATAAAGAGATTGAG
ATTGGAGTTCCCAATGCTCAGGACCGGCTAGATATTCTCCAGAAACTGCTTCGAAGGGTA
CCCCATTTGCTCACTGAGGCTGAGCTGCTGCAGCTGGCAAATAGTGCTCATGGATACGTT
GGAGCAGACTTGAAAGTCTTGTGTAATGAAGCAGGTCTCTGTGCCTTGCGGAGAATCCTG
AAAAAACAGCCTAACCTCCCTGATGTCAAGGTGGCTGGACTGGTGAAGATTACTCTGAAG
GATTTCTTTCAGGCAATGAATGATATCAGACCCAGCGCCATGAGGGAAATAGCAATTGAT
GTCCCAAATGTATCCTGGTCAGATATAGGAGGACTGGAAAGTATCAAACTGAAGTTGGAA
CAGGCTGTGGAATGGCCCTTAAAACATCCAGAGTCTTTCATTCGAATGGGTATTCAGCCA
CCTAAAGGAGTTCTTCTCTATGGGCCACCTGGGTGCTCTAAAACAATGATAGCAAAGGCT
TTGGCCAATGAGAGTGGACTGAATTTTCTAGCTATAAAGGGGCCTGAATTAATGAATAAA
TATGTTGGTGAATCCGAAAGAGCAGTTAGAGAGACCTTCCGAAAAGCAAGAGCAGTGGCG
CCTTCCATTATTTTCTTTGATGAACTGGATGCCTTAGCAGTTGAAAGGGGCAGTTCTTTA
GGTGCTGGGAATGTAGCCGATCGTGTTTTGGCTCAGCTCTTAACAGAAATGGATGGGATT
GAACAGCTAAAGGATGTGACCATTTTGGCAGCTACTAACCGTCCAGATAGGATAGACAAG
GCTTTGATGCGGCCTGGAAGAATTGATAGAATCATCTATGTGCCTTTACCGGATGCAGCA
ACAAGAAGGGAAATATTTAAGCTGCAGTTCCACTCCATGCCTGTCAGTAATGAAGTTGAC
CTGGATGAACTCATCCTTCAAACCGACGCATACTCAGGAGCAGAGATTGTAGCTGTCTGC
AGAGAGGCAGCTCTTCTGGCTCTGGAAGAAGGCATTCAAGCCAATCTCATCATGAAAAGA
CATTTCACTCAGGCCTTGAGCACTGTGACACCTAGAATTCCTGAGTCATTGAGACGTTTT
TATGAAGATTATCAAGAGAAGAGTGGGCTGCGTACACTC

Protein sequence predicted based on nucleotide sequence

MSSKKNRKRLNQSAENGSSLPSAASSCAEARAPSAGSDFAATSGTLTVTNLLEKVDDKIP
KTFQNPLIHLGLNTMKSANICIGRPVLLTSLNGKQEVYTAWPMAGFPGGKVGLSEMAQKN
VGVRPGDAIQVQPLVGAVLQAEEMDVALSDKDMEINEEELTGCILRKLDGKIVLPGNFLY
CTFYGRPYKLQVLRVKGADGMILGGPQSDPDTDAQRMAFEQSSMETSSLELSLQLSQLDL
EDTQIPTSRSTPYKPIDDRITNKASDVLLDVTQSPGDGSGLMLEEVTGLKCNFESAREGN
EQLTEEERLLKFSIGAKCNTDTFYFISSTTRVNFTEIDKNSKEQDNQFKVTYDMIGGLSS
QLKAIREIIELPLKQPELFKSYGIPAPRGVLLYGPPGTGKTMIARAVANEVGAYVSVING
PEIISKFYGETEAKLRQIFAEATLRHPSIIFIDELDALCPKREGAQNEVEKRVVASLLTL
MDGIGSEVSEGQVLVLGATNRPHALDAALRRPGRFDKEIEIGVPNAQDRLDILQKLLRRV
PHLLTEAELLQLANSAHGYVGADLKVLCNEAGLCALRRILKKQPNLPDVKVAGLVKITLK
DFFQAMNDIRPSAMREIAIDVPNVSWSDIGGLESIKLKLEQAVEWPLKHPESFIRMGIQP
PKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAVRETFRKARAVA
PSIIFFDELDALAVERGSSLGAGNVADRVLAQLLTEMDGIEQLKDVTILAATNRPDRIDK
ALMRPGRIDRIIYVPLPDAATRREIFKLQFHSMPVSNEVDLDELILQTDAYSGAEIVAVC
REAALLALEEGIQANLIMKRHFTQALSTVTPRIPESLRRFYEDYQEKSGLRTL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2751
Start on reference sequence 70
Mutations:
None
Discrepancy:
c196t,P66S; c628t,P210S;
 g838a,G280R; t1329c;
 t143
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable