PHLPPL (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PHLPP2
Gene Name:  PH domain and leucine rich repeat protein phosphatase 2
Original Clone ID:
FLH267133.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082509
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3969nts
Open reading frame: 1 to 3969

ATGAAACGCAATGGGAGCAGAAATTGTTTGAATAGGAGAAGTAGGTTTGGTTCTCGAGAA
AGAGACTGGCTAAGAGAAGATGTAAAGAGAGGCTGTGTTTACCTTTATGGAGCAGACACT
ACCACTGCCACTACAACCACCACCACCTCCTCTTCCTCTTCCTCCTCCTCCTCTTCCTCT
GACTTACATCTCGTCCTTTGCACTGTAGAGACACCAGCATCAGAAATATGTGCTGGAGAG
GGAAGAGAAAGTCTTTATTTACAGCTTCATGGAGACCTGGTCAGGAGACTGGAACCTACT
GAACGACCTCTTCAGATCGTTTATGATTACTTATCCAGGCTGGGATTTGATGATCCTGTG
CGCATACAGGAGGAGGCTACAAATCCTGACCTCGGCTGTATGATTCGATTTTATGGTGAA
AAACCATGCCACATGGATCGTTTGGATCGAATCCTATTGTCTGGCATCTATAATGTACGC
AAGGGAAAGACCCAGCTGCATAAGTGGGCTGAGCGCCTAGTTGTCCTCTGTGGTACCTGC
CTTATCGTTTCCTCAGTGAAGGATTGTCAAACTGGAAAGATGCACATTTTGCCTCTGGTT
GGTGGAAAGATAGAAGAAGTGAAGCGACGGCAATACTCCCTTGCTTTCAGCTCAGCAGGA
GCCCAAGCTCAGACCTATCATGTCAGCTTCGAGACTTTGGCCGAGTACCAGCGATGGCAA
CGGCAAGCATCCAAGGTGGTGTCCCAGCGAATCAGTACCGTGGATCTCTCGTGTTACAGC
CTCGAGGAGGTTCCTGAGCATCTCTTCTATAGTCAAGATATTACCTACCTCAACTTGCGA
CACAACTTCATGCAGTTAGAAAGACCCGGAGGCCTCGATACACTCTACAAATTTTCTCAA
CTGAAGGGCCTGAACTTGTCCCATAATAAACTTGGGTTGTTTCCTATATTGTTATGCGAG
ATCTCTACCCTGACTGAGCTCAACCTTTCCTGTAATGGATTTCATGACCTACCAAGTCAA
ATTGGCAATCTGCTAAATCTTCAAACCCTCTGTCTTGATGGCAACTTTCTGACTACTTTA
CCTGAAGAATTGGGAAATCTACAACAGCTTTCCTCCTTGGGAATTTCCTTCAACAACTTT
AGTCAAATTCCTGAGGTTTATGAGAAACTCACTATGTTAGATAGAGTGGTTATGGCAGGA
AATTGCCTGGAAGTCCTGAACTTAGGGGTGCTGAATAGGATGAACCATATCAAGCATGTG
GATTTAAGGATGAACCATTTGAAAACCATGGTTATTGAAAATCTGGAGGGAAATAAACAC
ATCACCCACGTGGATCTGCGGGACAACCGACTGACTGACTTGGATCTTAGCTCCTTATGC
AGCTTGGAACAGCTGCACTGTGGGCGGAATCAGCTGAGGGAGCTAACACTCAGTGGCTTT
TCCCTTCGGACCCTCTATGCCAGTTCCAACAGGCTGACAGCAGTGAACGTCTATCCAGTA
CCCAGCCTGCTCACTTTCTTGGATCTCTCCCGAAACCTGCTAGAGTGTGTCCCTGACTGG
GCCTGTGAAGCAAAGAAGATAGAAGTATTAGATGTGAGCTATAATCTTCTCACAGAGGTT
CCCGTGAGAATTCTGAGTAGCTTGAGTCTTAGAAAACTGATGCTGGGACACAATCATGTG
CAAAACCTTCCAACACTGGTAGAGCACATCCCCCTCGAGGTGCTGGATCTTCAGCATAAT
GCACTCACGAGGCTGCCAGACACCCTCTTCTCCAAGGCCTTAAATCTCAGATACTTGAAT
GCATCTGCAAATAGTCTGGAGTCTTTACCATCCGCCTGCACTGGAGAGGAGAGTTTGAGT
ATGCTGCAGCTGCTTTATCTGACCAACAATCTCCTGACGGATCAGTGCATACCTGTCCTG
GTAGGGCACCTGCACCTGCGAATCTTGCACCTTGCAAACAATCAGTTACAGACCTTTCCT
GCAAGCAAACTAAATAAATTGGAGCAATTGGAGGAACTGAACCTAAGTGGCAACAAGCTT
AAAACCATTCCCACAACCATAGCAAACTGTAAAAGGCTGCACACCCTTGTTGCACACTCC
AACAACATCAGCATTTTCCCAGAAATACTGCAGTTGCCTCAGATCCAGTTTGTAGACCTA
AGTTGCAACGACTTGACAGAAATCCTGATTCCAGAGGCTTTGCCTGCTACATTACAAGAC
CTTGACCTGACTGGAAATACAAATCTGGTTCTGGAACACAAGACACTGGACATATTTAGC
CATATCACAACCCTGAAAATTGATCAGAAACCTTTGCCAACCACAGATTCTACAGTTACG
TCAACCTTCTGGAGCCATGGACTGGCTGAGATGGCAGGGCAGAGAAATAAGCTGTGTGTC
TCAGCACTTGCTATGGATAGCTTTGCAGAGGGGGTGGGAGCTGTGTATGGCATGTTTGAT
GGAGACCGAAATGAGGAGCTCCCGCGCCTGCTGCAGTGTACGATGGCAGATGTGCTTTTA
GAAGAGGTACAGCAGTCAACTAATGACACAGTTTTCATGGCTAACACCTTCTTGGTATCT
CACAGGAAATTAGGAATGGCTGGCCAGAAGTTGGGCTCCTCCGCTCTCCTGTGCTACATC
CGCCCTGACACTGCCGATCCAGCAAGTAGCTTTAGCTTGACTGTAGCCAATGTTGGCACG
TGCCAAGCAGTCCTGTGCCGAGGTGGGAAGCCAGTGCCCCTCTCTAAAGTCTTCAGCCTG
GAGCAGGACCCAGAGGAGGCTCAAAGGGTGAAGGACCAAAAAGCCATCATCACAGAGGAC
AACAAAGTGAATGGGGTAACCTGCTGTACCCGGATGCTGGGCTGTACATACCTCTACCCT
TGGATCCTCCCCAAGCCCCACATATCTTCCACTCCGCTGACCATTCAAGATGAGTTGCTG
ATTCTGGGAAACAAAGCATTGTGGGAACACTTGTCCTACACAGAAGCTGTCAATGCTGTA
CGTCACGTACAAGACCCATTAGCAGCTGCTAAGAAGCTGTGCACATTAGCGCAGAGCTAT
GGCTGTCAGGACAATGTAGGGGCGATGGTAGTTTATTTGAATATTGGTGAGGAAGGCTGC
ACTTGTGAAATGAATGGGCTCACCCTCCCAGGTCCTGTGGGATTTGCTTCAACCACCACT
ATCAAGGATGCCCCTAAGCCAGCCACTCCATCCTCTAGCAGTGGGATTGCCTCTGAGTTC
AGCAGTGAGATGTCCACCTCAGAGGTGAGCAGTGAAGTGGGGTCCACTGCTTCTGATGAG
CATAATGCTGGGGGCCTGGACACTGCCTTGCTTCCGAGGCCAGAGCGGCGCTGCAGCCTC
CACCCAACACCCACCTCTGGGCTGTTTCAGCGCCAGCCTTCTTCTGCTACCTTCTCCAGT
AACCAGTCTGACAACGGCCTGGACAGTGATGATGACCAGCCCGTTGAGGGGGTCATAACC
AATGGCAGCAAGGTAGAGGTGGAAGTAGACATCCACTGCTGCAGGGGGAGGGATCTGGAG
AACTCACCCCCTCTCATAGAGAGTTCTCCTACCCTGTGTTCTGAGGAACATGCTAGAGGG
TCGTGTTTTGGGATCCGAAGACAGAACAGTGTGAATAGTGGCATGCTCCTGCCAATGAGC
AAGGACAGGATGGAGTTACAGAAGTCTCCCTCCACCTCCTGCCTCTATGGGAAGAAACTC
TCCAATGGCTCTATTGTGCCCCTAGAGGACAGCCTGAACCTCATTGAAGTGGCCACAGAA
GTGCCCAAGAGGAAAACTGGCTATTTTGCTGCCCCCACTCAGATGGAACCAGAGGACCAG
TTTGTTGTGCCTCATGACCTGGAAGAAGAAGTGAAGGAACAAATGAAACAGCACCAGGAC
AGCCGGCTCGAGCCTGAGCCCCATGAAGAGGATCGGACCGAGCCCCCGGAGGAGTTCGAC
ACAGCACTA

Protein sequence predicted based on nucleotide sequence

MKRNGSRNCLNRRSRFGSRERDWLREDVKRGCVYLYGADTTTATTTTTTSSSSSSSSSSS
DLHLVLCTVETPASEICAGEGRESLYLQLHGDLVRRLEPTERPLQIVYDYLSRLGFDDPV
RIQEEATNPDLGCMIRFYGEKPCHMDRLDRILLSGIYNVRKGKTQLHKWAERLVVLCGTC
LIVSSVKDCQTGKMHILPLVGGKIEEVKRRQYSLAFSSAGAQAQTYHVSFETLAEYQRWQ
RQASKVVSQRISTVDLSCYSLEEVPEHLFYSQDITYLNLRHNFMQLERPGGLDTLYKFSQ
LKGLNLSHNKLGLFPILLCEISTLTELNLSCNGFHDLPSQIGNLLNLQTLCLDGNFLTTL
PEELGNLQQLSSLGISFNNFSQIPEVYEKLTMLDRVVMAGNCLEVLNLGVLNRMNHIKHV
DLRMNHLKTMVIENLEGNKHITHVDLRDNRLTDLDLSSLCSLEQLHCGRNQLRELTLSGF
SLRTLYASSNRLTAVNVYPVPSLLTFLDLSRNLLECVPDWACEAKKIEVLDVSYNLLTEV
PVRILSSLSLRKLMLGHNHVQNLPTLVEHIPLEVLDLQHNALTRLPDTLFSKALNLRYLN
ASANSLESLPSACTGEESLSMLQLLYLTNNLLTDQCIPVLVGHLHLRILHLANNQLQTFP
ASKLNKLEQLEELNLSGNKLKTIPTTIANCKRLHTLVAHSNNISIFPEILQLPQIQFVDL
SCNDLTEILIPEALPATLQDLDLTGNTNLVLEHKTLDIFSHITTLKIDQKPLPTTDSTVT
STFWSHGLAEMAGQRNKLCVSALAMDSFAEGVGAVYGMFDGDRNEELPRLLQCTMADVLL
EEVQQSTNDTVFMANTFLVSHRKLGMAGQKLGSSALLCYIRPDTADPASSFSLTVANVGT
CQAVLCRGGKPVPLSKVFSLEQDPEEAQRVKDQKAIITEDNKVNGVTCCTRMLGCTYLYP
WILPKPHISSTPLTIQDELLILGNKALWEHLSYTEAVNAVRHVQDPLAAAKKLCTLAQSY
GCQDNVGAMVVYLNIGEEGCTCEMNGLTLPGPVGFASTTTIKDAPKPATPSSSSGIASEF
SSEMSTSEVSSEVGSTASDEHNAGGLDTALLPRPERRCSLHPTPTSGLFQRQPSSATFSS
NQSDNGLDSDDDQPVEGVITNGSKVEVEVDIHCCRGRDLENSPPLIESSPTLCSEEHARG
SCFGIRRQNSVNSGMLLPMSKDRMELQKSPSTSCLYGKKLSNGSIVPLEDSLNLIEVATE
VPKRKTGYFAAPTQMEPEDQFVVPHDLEEEVKEQMKQHQDSRLEPEPHEEDRTEPPEEFD
TAL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3978
Start on reference sequence 7
Mutations:
None
Discrepancy:
a116g,D39G; c439t,R147C;
 t462c; a2296g,K766E;
 t239
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable