Gene:
Gene Symbol:
NRAP
Gene Name:
nebulin-related anchoring protein
Original Clone ID:
FLH267143.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082519
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAATGTGCAGCCCTGTTCTAGGTGTGGGTATGGGGTTTATCCTGCCGAGAAGATCAGC
TGTATAGATCAGATATGGCATAAAGCCTGTTTTCACTGTGAAGTTTGCAAGATGATGCTG
TCTGTTAATAACTTTGTGAGTCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAG
AACAACACTTTCACCAGTGTCTATCACACTCCATTAAATCTAAATGTGAGGACATTTCCA
GAGGCCATCAGTGGGATCCATGACCAAGAAGATGGTGAACAGTGTAAATCAGTTTTTCAC
TGGGACATGAAATCCAAGGATAAGGAAGGTGCACCTAACAGGCAGCCACTGGCAAATGAG
AGAGCCTATTGGACTGGATATGGGGAAGGGAATGCTTGGTGCCCAGGAGCTCTGCCAGAC
CCCGAAATTGTAAGGATGGTTGAGGCTCGAAAGTCTCTTGGTGAGGAATATACAGAAGAC
TATGAGCAACCCAGGGGCAAGGGGAGCTTTCCAGCCATGATCACACCTGCTTATCAAAGG
GCCAAGAAAGCCAACCAGCTGGCCAGCCAAGTGGAGTATAAGAGAGGGCATGATGAACGC
ATCTCCAGGTTCTCCACGGTGGTGGATACTCCTGAGCTGCTACGGAGCAAGGCTGGGGCA
CAGCTTCAAAGTGATGTGAGATACACAGAGGACTATGAACAACAAAGAGGGAAAGGCAGT
TTCCCTGCGATGATCACACCCGCCTATCAGATAGCCAAAAGAGCCAATGAGCTGGCAAGT
GGTGTGAGGTACCATCAACAATATCAAAAAGAAATGAGGGGAATGGCTGGTCCAGCCATT
GGAGCTGAGGGCATCTTGACAAGGGAATGTGCAGACCAATATGGCCAGGGTTACCCGGAG
GAGTATGAGGAGCACAGGGGAAAGGGCAGCTTCCCAGCTATGATCACTCCAGCATATCAG
AACGCCAAGAAAGCTCACGAACTCGCTAGTGACATAAAATACAGGCAGGACTTCAATAAG
ATGAAAGGCGCTGCACATTATCACTCGCTTCCAGCTCAAGACAACTTGGTTCTCAAACAG
GCTCAGAGCGTAAACAAACTCGTGAGTGAGGTGGAGTATAAGAAGGATCTGGAAAGTAGT
AGAGGTCACAGTATCAACTACTGTGAAACACCTCAATTCAGGAACGTGAGCAAGATCTCA
AAATTTACCAGTGATAATAAATATAAAGAAAACTACCAGAACCACATGAGAGGCCGCTAT
GAAGGAGTTGGTATGGACAGACGCACTCTGCATGCTATGAAAGTTGGCAGCCTGGCAAGC
AACGTTGCCTACAAAGCTGATTATAAACATGATATTGTCGACTACAACTACCCAGCCACT
CTCACGCCTTCCTATCAAACAGCTATGAAACTGGTGCCCTTGAAAGATGCCAATTATAGG
CAGAGCATCGACAAGTTGAAGTACAGCTCGGTGACTGACACCCCACAGATTGTTCAAGCC
AAAATCAATGCCCAGCAGCTGAGTCATGTGAATTACCGTGCTGACTATGAGAAAAATAAG
TTGAATTACACATTGCCCCAGGATGTTCCTCAGCTGGTGAAGGCCAAAACCAATGCCAAA
CTCTTCAGTGAGGTTAAGTATAAAGAAGGCTGGGAGAAGACAAAGGGGAAAGGATTTGAG
ATGAAGCTGGATGCCATGTCTCTGCTGGCCGCCAAAGCCTCTGGGGAGCTTGCTAGCAAT
ATTAAATATAAAGAAGAATATGAAAAGACAAAAGGCAAAGCCATGGGAACAGCCGACTCT
AGGCTTCTGCACTCCCTGCAGATTGCTAAGATGAGCAGTGAGGTTGAATATAAGAAAGGC
TTTGAAGAGAGTAAGACCCGGTTTCACCTGCCCATGGATATGGTAAACATCAGGCATGCT
AAGAAGGCCCAAACTCTCGCCAGTGACCTGGACTACAGGAAGAAACTGCATGGATACACT
GTGCTGCCTGAAGATATGAAGACTCAGTGGGCCAAGAAGGCCTATGGGCTCCAGAGCGAG
CTGCAGTACAAGGCTGACCTGGCATGGATGAAGGGAGTTGGGTGGCTGACAGAGGGGAGT
CTCAACTTGGAACAAGCCAAGAAGGCTGGACAGCTGGTCAGCGAGAAAAACTACCGGCAG
AGGGTCGATGAGCTGAAGTTCACCAGCGTGACCGACAGCTCCCAGATGGAGCACGCCAAG
AAGAGCCAGGAGCTACAGAGCGGGGTGGCCTACAAGGCAGGAAATGAGCAGTCTGTCCAT
CAGTATACCATCAGCAAAGACGAGCCTCTCTTCCTGCAGGCCCGAGCCAATGCTGCAAAT
CTCAGCGAGAAACTGTATAAGAGCAGCTGGGAAAACCAGAAAGCAAAAGGGTTTGAGCTG
CGTCTTGATTCCTTGACCTTCCTGGCAGCCAAAGCCAAGAGGGACCTGGCTAGCGAGGTG
AAGTACAAGGAGGATTATGAGAGATCCAGAGGGAAGCTCATTGGGGCAAAAGATGTACAG
GGAGATTCGCAAATGAGCCACTCACTGCAAATGTCCAAGCTGCAGAGTGAGCTGGAGTAC
AAGGAGGGATTCGAGGACACCAAATCCCAATGCCACGTCTCCCTGGACATGGTCCACCTC
GTGCATGCCCGCAAAGCTCAGCATTTAGCCACAGACGTAGGCTACAAGACAGCGGAACAT
CACTTTACGGCTTTGCCCACAGACATGAAGGTGGAATGGGCCAAGAAGGCTTATGGCTTA
CAGAGTGATAACCAATACAGGGCAGATGTGAAGTGGATGAAAGGCATGGGCTGGGTCGCC
ACCGGGTCATTAAATGTGGAGCAGGCGAAGAAGGCAGGAGAACTCATTAGCGAGAAGAAG
TACCGTCAGCATCCAGATGCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAGATGGTC
CAGGCCAGAATTAGTTATACCCAAGCAGTGGATAGATTATACAGGGAACAAGGAGAAAAC
ATAAAGCATCATTACACACCGACTGCTGACCTCCCTGAAGTCCTGCTGGCCAAGCTGAAT
GCCATGAATATCAGTGAGACGCGTTATAAGGAATCCTGGAGCAAACTTCGAGATGGTGGC
TATAAACTGAGGTTGGATGCCCTTCCATTCCAAGCAGCAAAGGCTTCTGGTGAAATCATA
AGTGATTACAAATACAAAGAGGCATTTGAGAAAATGAAAGGACAGATGCTTGGTTCCCGG
AGCTTGGAAGATGATATCAGCCTTGCACATTCAGTGTATGCGACCTCACTGCAGAGTGAT
GTGAATTACAAGAAAGGCTTTGAACACTCAAAGGCGCAGTTCCATCTGCCGTTGGACATG
GCCGCCCTGGTGCATGCCAAGAAGGCTCAGACCCTGGCCAGCAATCAGGACTACAAACAT
CCACTGCCCCAGTACACTTCCTTGGCAGAAGACCTGAGGCTGAGCTGTGCCAAGAAAGCT
CACAAATTGCAGAGTGAGAATTTGTACCGGTCAGACCTGAACTTTATGCGAGGTGTTGCA
TGTGTCATTCCAGGCACGTTAGAGATTGAAGGGAGGAAGAAAGCATCGGAACTCATCAGT
GAGAGTAAATATCGGCAGCATCCCCACTCATTCAAGTACACAGCTGTGACAGACACTCCC
AACCTCCTTCATGCGAAATTCAGCAACCAGATAACGAATGAGCGCTTCTATAAAGCAGCT
GGAGAGGATGCAAGACACGAGTATACAATGACCCTGGGTCTGCCCGAGTTCATCCGAGCA
AAAACGAATGCAGCCAACCTGAGTGACGCAAGATACAAAGAGTCCTGGCGTAATCTTCGT
GCTCAAGGCTACAAGCTGACAATAGAAGCGCTCCCCTTCCAGGCTGCCCGGGCCTCTGGA
GATATAGCCAGTGATTTTCTCTACAGACATGACTTTGTGAAGGAGCGAGGGAAACTCATA
GGGCCCCAGAGTGTAAGAGACGACCCCCGGATCCAGCACTGCCGGCGCATGGGCCAGCTG
CAGAGCGAGCTTCAGTACAGGAGGGGGGCGACCAGCAGCCAAGCCCAGTTCCATCTGCCC
ATGGACATGGTGCACCTGGTCCATGCCAAGAATGCCCAGGCTCTGGCCAGCGACCACGAC
TACAGGACACAGTATCACAAGTTCACAGCACTGCCCGAGGACCTGAAGATGGCCTGGGCC
AAGAAAGCCCATGCCCTGCAGAGTGAGTTGCGCTACAAGTCAGACCTGATCGGCATGAAG
GGCATAGGATGGCTGGCGCTGAGATCCCCACAGATGGAGAGTGCAAAGAAGGCTGGAGAA
CTCATCAGCGAGACCAAGTACCGTAAAAAACCAGACAGTATCAAGTTCACCACAGTGGTT
GACTCCCCAGACCTGGTTCATGCCAAGAACAGCTATATGCACTGCAATGAGCGCATGTAT
AGATCTGGAGATGCAGAATCCCTGCACAGATACACCCTGATCCCCGACCATCCCGATTTC
ACCCGAGCTCGCCTCAATGCGCTGCATCTGAGTGACAAAGTCTACAGAAACTCCTGGGAG
CAGACCCGGGCTGGCAGTTATGACTTCAGGCCGGATGCCATCCCCTTCCAGACTGCCCGG
GCATCTAGGGAGATCGCCAGCGATTTCCGGTACAAAGAGGCTTTCCTGCGGGACCGAGGC
CTGCAGGTCGGGTACTGCAGTGTCGACGATGACCCAAGGATGAAGCATTTCCTCAACGTT
GGCAGGCTCCAGAGTGACAATGAGTACAAGAAGGACTTTGCCAAGAGTCGGTCCCAGTTT
CACAGCAGCACAGACCAGCACGGCCTCCTTCAGGCCAAGAGGAGCCAGCAGCTGGCCAGT
GATGTGCACTACAGGCAGCCCCTGCCCCAGCCCACCTGCGACCCGGAGCAGCTGGGCCTC
AGGCATGCTCAGAAGGCCCACCAGCTGCAGAGTGATGTCAAGTATAAATCAGACTTGAAC
CTGACCAGAGGTGTTGGCTGGACCCCTCCTGGCTCCTACAAAGTGGAAATGGCTCGGCGG
GCTGCGGAACTGGCCAACGCAAGGGGCCTGGGTCTCCAGGGAGCTTACCAGCGGGGGGCA
GAAGCAGTGGAGGCTGGAGATCATCAGAGTGGGGAGGTGAACCCAGATGCCACTGAGATT
CTGCACGTCAAAAAGAAGAAGGCCCTGCTGTTG
Protein sequence predicted based on nucleotide sequence
MNVQPCSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNNFVSHQKKPYCHAHNPK NNTFTSVYHTPLNLNVRTFPEAISGIHDQEDGEQCKSVFHWDMKSKDKEGAPNRQPLANE RAYWTGYGEGNAWCPGALPDPEIVRMVEARKSLGEEYTEDYEQPRGKGSFPAMITPAYQR AKKANQLASQVEYKRGHDERISRFSTVVDTPELLRSKAGAQLQSDVRYTEDYEQQRGKGS FPAMITPAYQIAKRANELASGVRYHQQYQKEMRGMAGPAIGAEGILTRECADQYGQGYPE EYEEHRGKGSFPAMITPAYQNAKKAHELASDIKYRQDFNKMKGAAHYHSLPAQDNLVLKQ AQSVNKLVSEVEYKKDLESSRGHSINYCETPQFRNVSKISKFTSDNKYKENYQNHMRGRY EGVGMDRRTLHAMKVGSLASNVAYKADYKHDIVDYNYPATLTPSYQTAMKLVPLKDANYR QSIDKLKYSSVTDTPQIVQAKINAQQLSHVNYRADYEKNKLNYTLPQDVPQLVKAKTNAK LFSEVKYKEGWEKTKGKGFEMKLDAMSLLAAKASGELASNIKYKEEYEKTKGKAMGTADS RLLHSLQIAKMSSEVEYKKGFEESKTRFHLPMDMVNIRHAKKAQTLASDLDYRKKLHGYT VLPEDMKTQWAKKAYGLQSELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQ RVDELKFTSVTDSSQMEHAKKSQELQSGVAYKAGNEQSVHQYTISKDEPLFLQARANAAN LSEKLYKSSWENQKAKGFELRLDSLTFLAAKAKRDLASEVKYKEDYERSRGKLIGAKDVQ GDSQMSHSLQMSKLQSELEYKEGFEDTKSQCHVSLDMVHLVHARKAQHLATDVGYKTAEH HFTALPTDMKVEWAKKAYGLQSDNQYRADVKWMKGMGWVATGSLNVEQAKKAGELISEKK YRQHPDALKFTSIKDTPEMVQARISYTQAVDRLYREQGENIKHHYTPTADLPEVLLAKLN AMNISETRYKESWSKLRDGGYKLRLDALPFQAAKASGEIISDYKYKEAFEKMKGQMLGSR SLEDDISLAHSVYATSLQSDVNYKKGFEHSKAQFHLPLDMAALVHAKKAQTLASNQDYKH PLPQYTSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVACVIPGTLEIEGRKKASELIS ESKYRQHPHSFKYTAVTDTPNLLHAKFSNQITNERFYKAAGEDARHEYTMTLGLPEFIRA KTNAANLSDARYKESWRNLRAQGYKLTIEALPFQAARASGDIASDFLYRHDFVKERGKLI GPQSVRDDPRIQHCRRMGQLQSELQYRRGATSSQAQFHLPMDMVHLVHAKNAQALASDHD YRTQYHKFTALPEDLKMAWAKKAHALQSELRYKSDLIGMKGIGWLALRSPQMESAKKAGE LISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERMYRSGDAESLHRYTLIPDHPDF TRARLNALHLSDKVYRNSWEQTRAGSYDFRPDAIPFQTARASREIASDFRYKEAFLRDRG LQVGYCSVDDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSSTDQHGLLQAKRSQQLAS DVHYRQPLPQPTCDPEQLGLRHAQKAHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARR AAELANARGLGLQGAYQRGAEAVEAGDHQSGEVNPDATEILHVKKKKALLL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
116
End on reference sequence
5311
Mutations:
None
Discrepancy:
g409a,A137T; a2254g,K752E;
g3334t,A1112S; t3920c,L
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
