DDX59 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DDX59
Gene Name:  DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
Original Clone ID:
FLH267187.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082563
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1857nts
Open reading frame: 1 to 1857

ATGTTTGTTCCAAGATCTCTAAAAATCAAGAGGAATGCTAATGATGATGGCAAAAGTTGT
GTGGCTAAGATAATTAAACCAGACCCAGAAGACCTTCAGTTGGACAAAAGCAGAGATGTT
CCCGTTGATGCTGTAGCTACAGAAGCAGCCACAATAGACAGGCACATCAGCGAATCATGC
CCTTTCCCCAGCCCAGGTGGCCAGTTGGCAGAGGTTCATTCAGTAAGTCCCGAGCAGGGT
GCGAAGGACAGCCATCCTTCTGAAGAGCCCGTTAAGTCATTTTCCAAAACACAGCGCTGG
GCAGAACCAGGGGAACCCATCTGTGTTGTCTGTGGTCGTTATGGAGAGTATATCTGTGAT
AAGACAGATGAAGATGTGTGTAGTTTGGAGTGTAAAGCGAAACATCTTCTACAAGTTAAG
GAAAAGGAAGAGAAATCAAAACTCAGCAATCCACAGAAGGCTGATTCTGAGCCAGAGTCT
CCACTGAATGCTTCCTATGTCTACAAAGAGCACCCCTTTATTTTGAACCTTCAGGAAGAC
CAGATTGAAAATCTTAAACAGCAGCTGGGAATTTTAGTTCAAGGGCAAGAAGTCACCAGG
CCCATTATTGACTTTGAACATTGTAGTCTCCCTGAGGTCTTAAATCACAACTTGAAGAAA
TCAGGCTATGAGGTGCCAACTCCCATTCAAATGCAGATGATTCCTGTGGGACTTCTGGGA
AGAGACATTCTGGCCAGTGCAGATACTGGCTCAGGAAAAACAGCTGCTTTTCTTCTTCCT
GTTATCATGCGAGCTTTATTCGAGAGCAAAACTCCATCTGCGCTCATTCTTACACCAACC
AGAGAGTTAGCCATTCAGATAGAGAGACAAGCTAAAGAATTGATGAGTGGCCTGCCACGC
ATGAAAACTGTGCTTCTTGTAGGGGGCTTACCCTTACCCCCACAGCTTTATCGTCTGCAA
CAACATGTTAAGGTTATCATAGCAACCCCTGGGCGACTTCTGGATATAATAAAGCAGAGC
TCTGTAGAACTCTGTGGTGTAAAGATTGTGGTAGTAGATGAAGCTGATACCATGTTAAAG
ATGGGTTTTCAACAACAAGTGCTTGACATTTTGGAAAACATTCCTAATGATTGTCAGACC
ATTTTGGTTTCAGCCACAATTCCAACTAGCATAGAACAGCTAGCAAGCCAGCTTCTGCAT
AATCCTGTGAGAATTATCACTGGAGAAAAGAACCTACCTTGTGCCAATGTACGTCAGATT
ATTTTGTGGGTAGAAGACCCAGCCAAAAAGAAAAAATTATTTGAAATTTTAAATGATAAG
AAACTCTTTAAGCCTCCAGTGTTAGTATTTGTGGACTGCAAACTAGGAGCAGATCTTTTG
AGTGAAGCCGTTCAGAAAATCACAGGGCTGAAAAGAATATCTATACATTCGGAGAAGTCG
CAAATAGAAAGGAAAAACATATTGAAGGGATTACTTGAAGGAGACTATGAAGTTGTAGTG
AGCACAGGAGTCTTGGGACGAGGCCTAGACTTGATCAGTGTCAGGCTGGTTGTCAATTTT
GATATGCCTTCAAGTATGGATGAGTATGTCCATCAGATTGGAAGAGTAGGAAGATTAGGT
CAAAATGGAACAGCGATTACTTTCATCAATAATAATTCAAAAAGACTCTTCTGGGATATT
GCAAAACGAGTAAAGCCCACAGGATCCATTCTTCCCCCTCAGTTATTAAATTCCCCATAC
CTTCATGACCAGAAGAGAAAGGAACAACAGAAAGATAAACAGACACAGAATGATCTGGTT
ACAGGAGCTAATCTTATGGATATTATCAGAAAACATGATAAAAGTAATTCTCAGAAA

Protein sequence predicted based on nucleotide sequence

MFVPRSLKIKRNANDDGKSCVAKIIKPDPEDLQLDKSRDVPVDAVATEAATIDRHISESC
PFPSPGGQLAEVHSVSPEQGAKDSHPSEEPVKSFSKTQRWAEPGEPICVVCGRYGEYICD
KTDEDVCSLECKAKHLLQVKEKEEKSKLSNPQKADSEPESPLNASYVYKEHPFILNLQED
QIENLKQQLGILVQGQEVTRPIIDFEHCSLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLG
RDILASADTGSGKTAAFLLPVIMRALFESKTPSALILTPTRELAIQIERQAKELMSGLPR
MKTVLLVGGLPLPPQLYRLQQHVKVIIATPGRLLDIIKQSSVELCGVKIVVVDEADTMLK
MGFQQQVLDILENIPNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQI
ILWVEDPAKKKKLFEILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKRISIHSEKS
QIERKNILKGLLEGDYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQIGRVGRLG
QNGTAITFINNNSKRLFWDIAKRVKPTGSILPPQLLNSPYLHDQKRKEQQKDKQTQNDLV
TGANLMDIIRKHDKSNSQK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2094
Start on reference sequence 235
Mutations:
None
Discrepancy:
t1438c,S480P
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable