Gene:
Gene Symbol:
ARHGAP4
Gene Name:
Rho GTPase activating protein 4
Original Clone ID:
FLH267196.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082572
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCGCTCACGGGAAGCTGCGGCGGGAGCGGGGGCTGCAGGCTGAGTATGAGACGCAA
GTCAAAGAGATGCGCTGGCAGCTGAGCGAGCAGCTGCGCTGCCTGGAGCTGCAGGGCGAG
CTGCGGCGGGAGTTGCTGCAGGAGCTGGCAGAGTTCATGCGGCGCCGCGCTGAGGTGGAG
CTGGAATACTCCCGGGGCCTGGAAAAGCTGGCCGAGCGCTTCTCCAGCCGTGGAGGCCGC
CTGGGGAGCAGCCGGGAGCACCAAAGCTTCCGGAAGGAGCCGTCCCTCCTGTCGCCCTTG
CACTGCTGGGCGGTGCTGCTGCAGCACACGCGGCAGCAGAGCCGGGAGAGCGCGGCCCTG
AGTGAGGTGCTGGCCGGGCCCCTGGCCCAGCGCCTGAGTCACATTGCAGAGGACGTGGGG
CGCCTGGTCAAGAAGAGCAGGGATCTGGAGCAGCAGCTGCAGGATGAGCTCCTGGAGGTG
GTCTCAGAGCTCCAGACGGCCAAGAAGACGTACCAGGCATATCACATGGAGAGCGTGAAT
GCCGAGGCCAAGCTCCGGGAGGCCGAGCGGCAGGAGGAGAAGCGGGCAGGCCGGAGTGTC
CCCACCACCACCGCTGGTGCCACTGAGGCAGGGCCCCTCCGCAAGAGCTCCCTCAAGAAG
GGAGGGAGGCTGGTGGAGAAGCTCTGGCCCCCGCAGAGGCCTGTGGCCGCTTCCAGCTGT
GCACCTGTGTGCTGGCTCCAAGCTGGGTTTCTCGTGCACCCTCCATGGTGGGGTGCCATG
TGCGCACCTTCCACTCATCAGCGGCAGGCCAAGTTCATGGAGCACAAACTCAAGTGCACA
AAGGCGCGCAACGAGTACCTGCTTAGCCTGGCTAGTGTCAACGCTGCTGTCAGTAACTAC
TACCTGCATGACGTCTTGGACCTCATGGACTGCTGTGACACAGGGTTCCACCTGGCCCTG
GGGCAGGTGCTCCGGAGCTACACGGCCGCTGAGAGCCGCACCCAAGCCTCCCAAGTGCAG
GGCCTGGGCAGCCTGGAAGAAGCTGTGGAGGCCCTGGATCCTCCAGGGGACAAAGCCAAG
GTTCTCGAGGTGCATGCTACCGTCTTCTGTCCCCCGCTGCGCTTTGACTACCACCCCCAT
GATGGGGATGAGGTGGCTGAGATCTGCGTTGAAATGGAGCTGCGGGACGAGATTCTGCCC
AGAGCCCAGAACATCCAGAGCCGCCTGGACCGACAGACCATTGAGACAGAGGAGGTGAAC
AAGACTCTGAAGGCGACACTGCAGGCCCTGCTGGAGGTGGTGGCCTCGGATGACGGGGAT
GTGCTTGATTCCTTCCAGACCAGCCCCTCCACCGAGTCCCTCAAGTCCACCAGCTCAGAC
CCAGGCAGCCGGCAGGCGGGCCGGAGGCGCGGCCAGCAGCAGGAGACCGAAACCTTCTAC
CTCACGAAGCTCCAGGAGTATCTGAGTGGACGGAGCATCCTCGCCAAGCTGCAGGCCAAG
CACGAGAAGCTGCAGGAGGCCCTTCAGCGAGGTGACAAGGAGGAGCAGGAGGTGTCTTGG
ACCCAGTACACACAGAGAAAATTCCAGAAGAGCCGCCAGCCCCGCCCCAGCTCCCAGTAT
AACCAGAGACTCTTTGGGGGAGACATGGAGAAGTTTATCCAGAGCTCAGGCCAGCCTGTG
CCCCTGGTGGTGGAGAGCTGCATTCGCTTCATCAACCTCAATGGCCTGCAGCATGAAGGC
ATCTTCCGGGTATCGGGTGCCCAGCTCCGGGTCTCAGAGATCCGTGATGCCTTCGAGAGA
GGGGAGGACCCACTGGTGGAGGGCTGCACTGCCCACGACCTGGACTCGGTGGCCGGGGTG
CTGAAGCTCTACTTCCGGAGCCTGGAGCCCCCACTCTTCCCCCCAGACCTGTTCGGCGAG
CTGCTGGCTTCTTCGGAGCTGGAGGCCACAGCGGAGAGGGTGGAGCACGTGAGCCGCCTG
CTGTGGCGGCTGCCCGCGCCGGTGCTGGTGGTTCTGCGCTACCTCTTCACCTTCCTCAAC
CACCTGGCCCAGTACAGCGATGAGAACATGATGGACCCCTACAACCTGGCCGTGTGCTTC
GGGCCCACGCTGCTACCGGTGCCCGCTGGGCAGGACCCGGTGGCGCTGCAGGGCCGGGTG
AACCAGCTGGTGCAGACGCTCATAGTGCAGCCCGATCGGGTCTTCCCGCCCCTGACCTCG
CTGCCTGGCCCCGTCTACGAGAAGTGCATGGCACCGCCTTCCGCCAGCTGCCTGGGGGAC
GCCCAGCTGGAGAGCCTGGGGGCGGACAATGAGCCGGAGCTGGAAGCCGAGATGCCCGCA
CAGGAGGATGACCTGGAGGGGGTCGTGGAGGCTGTGGCCTGCTTTGCCTACACGGGCCGC
ACAGCCCAGGAGCTGAGCTTCCGGCGGGGGGACGTACTGCGGCTGCACGAGAGGGCCTCG
AGCGACTGGTGGCGGGGGGAGCACAACGGCATGCGGGGCCTCATCCCCCACAAGTATATC
ACGCTGCCCGCCGGGACGGAGAAGCAGGTGGTGGGCGCAGGGCTGCAGACTGCAGGGGAG
TCTGGGAGCAGTCCCGAGGGCCTCCTGGCATCGGAGCTGGTCCACCGGCCAGAGCCATGC
ACCTCACCTGAGGCCATGGGACCCTCTGGACACAGACGACGCTGCTTGGTCCCAGCCTCC
CCAGAGCAACACGTGGAGGTGGATAAGGCTGTGGCACAGAACATGGACTCTGTGTTTAAG
GAGCTCTTGGGAAAGACCTCTGTCCGCCAGGGCCTTGGGCCAGCATCTACCACCTCTCCC
AGTCCTGGGCCCCGAAGCCCAAAGGCCCCGCCCAGCAGCCGCCTGGGCAGGAACAAAGGC
TTCTCCCGGGGCCCTGGGGCCCCAGCCTCACCCTCAGCTTCCCACCCCCAGGGCCTAGAC
ACGACCCCCAAGCCACAC
Protein sequence predicted based on nucleotide sequence
MAAHGKLRRERGLQAEYETQVKEMRWQLSEQLRCLELQGELRRELLQELAEFMRRRAEVE LEYSRGLEKLAERFSSRGGRLGSSREHQSFRKEPSLLSPLHCWAVLLQHTRQQSRESAAL SEVLAGPLAQRLSHIAEDVGRLVKKSRDLEQQLQDELLEVVSELQTAKKTYQAYHMESVN AEAKLREAERQEEKRAGRSVPTTTAGATEAGPLRKSSLKKGGRLVEKLWPPQRPVAASSC APVCWLQAGFLVHPPWWGAMCAPSTHQRQAKFMEHKLKCTKARNEYLLSLASVNAAVSNY YLHDVLDLMDCCDTGFHLALGQVLRSYTAAESRTQASQVQGLGSLEEAVEALDPPGDKAK VLEVHATVFCPPLRFDYHPHDGDEVAEICVEMELRDEILPRAQNIQSRLDRQTIETEEVN KTLKATLQALLEVVASDDGDVLDSFQTSPSTESLKSTSSDPGSRQAGRRRGQQQETETFY LTKLQEYLSGRSILAKLQAKHEKLQEALQRGDKEEQEVSWTQYTQRKFQKSRQPRPSSQY NQRLFGGDMEKFIQSSGQPVPLVVESCIRFINLNGLQHEGIFRVSGAQLRVSEIRDAFER GEDPLVEGCTAHDLDSVAGVLKLYFRSLEPPLFPPDLFGELLASSELEATAERVEHVSRL LWRLPAPVLVVLRYLFTFLNHLAQYSDENMMDPYNLAVCFGPTLLPVPAGQDPVALQGRV NQLVQTLIVQPDRVFPPLTSLPGPVYEKCMAPPSASCLGDAQLESLGADNEPELEAEMPA QEDDLEGVVEAVACFAYTGRTAQELSFRRGDVLRLHERASSDWWRGEHNGMRGLIPHKYI TLPAGTEKQVVGAGLQTAGESGSSPEGLLASELVHRPEPCTSPEAMGPSGHRRRCLVPAS PEQHVEVDKAVAQNMDSVFKELLGKTSVRQGLGPASTTSPSPGPRSPKAPPSSRLGRNKG FSRGPGAPASPSASHPQGLDTTPKPH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3019
Start on reference sequence
59
Mutations:
None
Discrepancy:
t458c,L153P
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
