MIB2 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MIB2
Gene Name:  mindbomb E3 ubiquitin protein ligase 2
Original Clone ID:
FLH267209.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082585
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2997nts
Open reading frame: 1 to 2997

ATGGGTTGGAAGCCCAGCGAGGCTAGAGGCCAGTCCCAAAGTTTCCAGGCATCAGGGCTG
CAGCCCAGGAGCCTCAAGGCGGCCCGGCGGGCGACTGGACGGCCGGACAGGTCCCGAGCA
GCCCCGCCCAACATGGACCCAGACCCCCAGGCGGGCGTGCAGGTGGGCATGCGGGTGGTG
CGCGGCGTGGACTGGAAGTGGGGCCAGCAGGACGGCGGCGAGGGCGGCGTGGGCACGGTG
GTGGAGCTTGGCCGCCACGGCAGCCCCTCGACACCCGACCGCACAGTGGTCGTGCAGTGG
GACCAGGGCACGCGCACCAACTACCGCGCCGGCTACCAGGGCGCGCACGACCTGCTGCTG
TACGACAACGCCCAGATCGGCGTCCGGCACCCCAACATCATCTGTGACTGCTGCAAGAAG
CACGGGCTGCGGGGGATGCGCTGGAAGTGCCGTGTGTGCCTGGACTACGACCTCTGCACG
CAGTGCTACATGCACAACAAGCATGAGCTCGCCCACGCCTTCGACCGCTACGAGACCGCT
CACTCGCGCCCTGTCACACTGAGTCCCCGCCAGGGCCTCCCGAGGATCCCACTAAGGGGC
ATCTTCCAGGGAGCGAAGGTGGTGCGAGGCCCCGACTGGGAGTGGGGCTCACAGGATGGA
GGGGAAGGGAAACCGGGCCGTGTGGTGGACATCCGTGGCTGGGATGTGGAGACAGGCCGG
AGTGTGGCCAGCGTGACGTGGGCTGATGGTACCACCAATGTGTACCGTGTGGGCTACAAG
GGCAAGGTGGACCTCAAGTGTGTGGGCGAGGCAGCGGGCGGCTTCTACTACAAGGACCAC
CTCCCAAGGCTCGGCAAGCCGGCGGAGCTGCAGCGCAGGGTGAGTGCTGACAGCCAGCCC
TTCCAGCACGGGGACAAGGTCAAGTGTCTGCTGGACACTGATGTCCTGCGGGAGATGCAG
GAAGGCCACGGCGGCTGGAACCCCAGGATGGCGGAGTTTATCGGACAGACGGGCACCGTG
CATCGTATCACGGACCGCGGGGACGTGCGCGTGCAGTTCAACCACGAGACGCGCTGGACC
TTCCACCCCGGGGCGCTCACCAAGCACCACTCCTTCTGGGTGGGCGACGTGGTCCGGGTC
ATCGGCGACCTTGACACAGTGAAGCGGCTGCAGGCTGGGCATGGCGAGTGGACGGACGAC
ATGGCCCCTGCCCTGGGCCGCGTCGGGAAGGTGGTGAAAGTGTTTGGAGACGGGAACCTG
CGTGTAGCAGTCGCTGGTCAGCGGTGGACCTTCAGCCCCTCCTGCCTGGTGGCCTACCGG
CCCGAGGAGGATGCCAACCTGGACGTGGCCGAGCGCGCCCGGGAGAACAAAAGCTCACTG
AGCGTGGCCCTGGACAAGCTTCGGGCCCAGAAGAGTGACCCAGAGCACCCGGGAAGGCTG
GTGGTGGAGGTGGCGCTGGGTAACGCAGCCCGGGCTCTGGGCCTGCTGCGGAGGCGCCCA
GAGCAGGTGGACACCAAGAACCAAGGCAGGACCGCTCTGCAAGTGGCTGCCTACCTGGGC
CAGGTGGAGTTGATACGGCTGCTGCTACAAGCCAGGGCGGGCGTGGACCTGCCGGACGAC
GAGGGCAACACGGCACTGCACTACGCGGCCCTGGGGAACCAGCCCGAGGCCACCAGGGTG
CTCCTGAGTGCTGGGTGCCGGGCGGACGCCATCAACAGCACCCAGAGCACAGCACTGCAC
GTGGCCGTGCAGAGGGGCTTCCTGGAGGTGGTGCGGGCCCTGTGTGAGCGCGGCTGTGAC
GTCAACCTGCCCGACGCCCACTCGGACACGCCCCTGCACTCCGCCATCTCGGCGGGCACT
GGAGCCGGCGGCATTGTCGAGGTCCTCACGGAGGTGCCAAACATCGATGTTACCGCCACC
AACAGCCAGGGTTTCACCCTGCTGCACCATGCCTCCCTCAAGGGTCACGCGCTAGCTGTG
AGAAAGATTCTGGCTCGGGCGCGGCAGCTGGTGGACGCCAAGAAGGAGGACGGCTTCACG
GCGCTGCATCTGGCTGCCCTCAACAACCACCGCGAGGTGGCCCAGATCCTCATCCGGGAG
GGCCGCTGTGACGTGAACGTGCGCAACCGGAAGCTGCAGTCCCCGCTGCATCTCGCCGTG
CAACAGGCCCACGTGGGGCTGGTGCCGCTACTGGTGGACGCTGGGTGCAGTGTCAACGCC
GAGGACGAGGAGGGGGACACAGCCCTGCACGTGGCGCTGCAGCGTCATCAGCTGCTGCCC
CTGGTGGCTGATGGGGCCGGGGGGGACCCAGGGCCCTTGCAGCTGCTGTCCAGGCTACAG
GCCTCGGGCCTCCCCGGCAGCGCGGAGCTGACGGTGGGCGCGGCGGTCGCCTGCTTCCTG
GCGCTGGAGGGCGCCGACGTGAGCTACACCAACCACCGCGGTCGGAGCCCGCTGGACCTG
GCCGCCGAGGGTCGCGTGCTCAAGGCCCTTCAGGGCTGCGCCCAGCGCTTCCGGGAGCGG
CAGGCGGGCGGGGGCGCGGCCCCGGGCCCCAGGCAAACGCTCGGGACCCCCAACACCGTG
ACGAACCTGCACGTGGGCGCCGCGCCGGGGCCCGAGGCCGCTGAGTGCCTGGTGTGCTCC
GAGCTGGCGCTGCTGGTGCTGTTCTCGCCGTGCCAGCACCGCACCGTGTGTGAGGAGTGC
GCGCGCAGGATGAAGAAGTGCATCAGGTGCCAGGTGGTCGTCAGCAAGAAACTGCGCCCA
GACGGCTCTGAGGTGGCGAGCGCCGCCCCCGCCCCCGGCCCGCCGCGCCAGCTGGTGGAG
GAGCTGCAGAGCCGCTACCGGCAGATGGAGGAACGCATCACCTGCCCCATCTGCATCGAC
AGCCACATCCGCCTCGTGTTCCAGTGCGGCCACGGCGCATGCGCCCCCTGCGGCTCCGCG
CTCAGCGCCTGCCCCATCTGCCGCCAGCCCATCCGCGACCGCATCCAGATCTTCGTG

Protein sequence predicted based on nucleotide sequence

MGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAAPPNMDPDPQAGVQVGMRVV
RGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLL
YDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETA
HSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGR
SVASVTWADGTTNVYRVGYKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQP
FQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWT
FHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNL
RVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRL
VVEVALGNAARALGLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDD
EGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCD
VNLPDAHSDTPLHSAISAGTGAGGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAV
RKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAV
QQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQ
ASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRER
QAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEEC
ARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICID
SHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 139
End on reference sequence 3138
Mutations:
None
Discrepancy:
a2779g,S927G
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable