Gene:
Gene Symbol:
TLL2
Gene Name:
tolloid-like 2
Original Clone ID:
FLH267377.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082600
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCCCGGGCGACTGCACTTGGGGCCCTGGTGTCACTGCTGCTGCTGCTGCCGCTGCCT
CGCGGCGCCGGGGGACTCGGGGAGCGCCCGGACGCCACCGCAGACTACTCAGAGCTGGAC
GGCGAGGAGGGCACGGAGCAGCAGCTGGAGCATTACCACGACCCTTGCAAAGCCGCTGTC
TTTTGGGGAGACATTGCCTTAGATGAAGATGACTTGAAGCTGTTTCACATTGACAAAGCC
AGAGACTGGACCAAGCAGACAGTGGGGGCAACAGGACACAGCACAGGTGGGCTTGAAGAG
CAGGCATCTGAGAGCAGCCCAGACACCACAGCCATGGACACTGGCACCAAGGAAGCTGGA
AAGGATGGCCGGGAGAATACCACACTCCTGCACAGCCCTGGGACCTTGCATGCCGCAGCC
AAGACCTTCTCTCCCCGGGTCCGAAGAGCCACAACCTCAAGGACAGAGAGGATATGGCCT
GGAGGAGTCATCCCCTACGTCATTGGAGGGAACTTCACTGGGAGCCAGAGGGCCATTTTT
AAGCAGGCCATGAGACACTGGGAGAAGCACACCTGTGTGACCTTCATAGAAAGGACGGAT
GAGGAAAGCTTTATTGTATTCAGTTACAGAACCTGTGGCTGTTGCTCCTATGTTGGGCGC
CGAGGAGGAGGCCCACAGGCCATATCCATTGGGAAGAACTGTGACAAGTTTGGCATTGTG
GCTCACGAGCTGGGCCATGTGGTTGGGTTTTGGCATGAACACACCCGGCCAGACAGAGAC
CAACATGTCACCATCATCAGGGAAAACATCCAGCCAGGTCAGGAGTATAATTTCTTAAAA
ATGGAAGCTGGGGAAGTGAGCTCTCTGGGAGAGACATACGACTTTGACAGCATCATGCAC
TACGCCCGGAACACCTTCTCAAGAGGAGTTTTCTTAGACACCATCCTTCCCCGTCAAGAT
GACAATGGCGTCAGGCCAACCATTGGCCAGCGCGTGCGGCTCAGTCAGGGAGACATAGCT
CAAGCCCGGAAGCTGTACAAATGCCCAGCGTGTGGGGAGACCCTGCAGGACACAACGGGA
AACTTTTCTGCACCTGGTTTCCCAAATGGGTACCCATCTTACTCCCACTGCGTCTGGAGG
ATCTCGGTCACCCCAGGGGAAAAGATCGTATTAAACTTCACATCCATGGATTTGTTTAAA
AGCCGACTGTGCTGGTATGATTACGTGGAGGTCCGGGATGGTTACTGGAGAAAAGCCCCC
CTTTTGGGCAGGTTTTGTGGCGATAAGATCCCGGAGCCCCTCGTCTCCACGGACAGCCGG
CTCTGGGTGGAGTTCCGCAGCAGCAGCAACATCTTGGGCAAGGGCTTCTTTGCAGCGTAC
GAAGCTACCTGCGGGGGAGACATGAACAAAGATGCCGGTCAGATTCAATCTCCCAACTAT
CCGGATGACTACAGACCTTCCAAGGAATGTGTCTGGAGGATTACGGTTTCAGAGGGGTTT
CACGTGGGACTTACCTTCCAAGCTTTTGAGATTGAAAGGCACGACAGCTGTGCATATGAC
TACCTGGAAGTCCGGGATGGCCCCACGGAAGAGAGTGCCCTGATCGGCCACTTTTGTGGC
TATGAGAAGCCGGAGGATGTGAAATCGAGCTCCAACAGACTGTGGATGAAGTTTGTGTCC
GATGGCTCTATCAATAAAGCGGGCTTTGCAGCCAATTTTTTCAAGGAGGTGGATGAGTGT
TCCTGGCCAGATCACGGCGGGTGCGAGCATCGCTGTGTGAACACGCTGGGCAGCTACAAG
TGTGCCTGTGACCCTGGCTACGAGCTGGCCGCCGATAAGAAGATGTGTGAAGTGGCCTGT
GGCGGTTTCATTACCAAGCTGAATGGAACCATCACCAGCCCTGGGTGGCCGAAGGAGTAT
CCCACAAACAAAAACTGTGTCTGGCAGGTGGTGGCCCCCGCTCAGTACCGGATCTCCCTT
CAGTTTGAAGTGTTTGAACTGGAAGGCAATGACGTCTGTAAGTACGACTTTGTAGAGGTG
CGCAGCGGCCTGTCCCCCGACGCCAAGCTGCACGGCAGGTTCTGCGGCTCTGAGACGCCG
GAGGTCATCACCTCGCAGAGCAACAACATGCGCGTGGAGTTCAAGTCCGACAACACCGTC
TCCAAGCGCGGCTTCAGGGCCCACTTCTTCTCAGATAAGGACGAGTGTGCCAAGGACAAC
GGCGGGTGTCAGCATGAGTGCGTCAACACCTTCGGGAGCTACCTGTGCAGGTGCAGAAAC
GGCTACTGGCTCCACGAGAATGGGCATGACTGCAAAGAGGCTGGCTGTGCACACAAGATC
AGCAGTGTGGAGGGGACCCTGGCGAGCCCCAACTGGCCTGACAAATACCCCAGCCGGAGG
GAGTGTACCTGGAACATCTCTTCGACTGCAGGCCACAGAGTGAAACTCACCTTTAATGAG
TTTGAGATCGAGCAGCACCAGGAATGTGCCTATGACCACCTGGAAATGTATGACGGGCCG
GACAGCCTGGCCCCCATTCTGGGCCGTTTCTGCGGCAGCAAGAAACCAGACCCCACGGTG
GCTTCCGGCAGCAGTATGTTTCTCAGGTTTTATTCGGATGCCTCAGTGCAGAGGAAAGGC
TTCCAGGCAGTGCACAGCACAGAGTGCGGGGGCAGGCTGAAGGCTGAAGTGCAGACCAAA
GAGCTCTATTCCCACGCCCAGTTTGGGGACAACAACTACCCGAGCGAGGCCCGCTGTGAC
TGGGTGATCGTGGCAGAGGACGGCTACGGCGTGGAGCTGACATTCCGGACCTTTGAGGTT
GAGGAGGAGGCCGACTGCGGCTACGACTACATGGAAGCCTACGACGGCTACGACAGCTCA
GCGCCCAGGCTCGGCCGCTTCTGTGGCTCTGGGCCATTAGAAGAAATCTACTCTGCAGGT
GATTCCCTGATGATTCGATTCCGCACAGATGACACCATCAACAAGAAAGGCTTTCATGCC
CGATACACCAGCACCAAGTTCCAGGATGCCCTGCACATGAAGAAA
Protein sequence predicted based on nucleotide sequence
MPRATALGALVSLLLLLPLPRGAGGLGERPDATADYSELDGEEGTEQQLEHYHDPCKAAV FWGDIALDEDDLKLFHIDKARDWTKQTVGATGHSTGGLEEQASESSPDTTAMDTGTKEAG KDGRENTTLLHSPGTLHAAAKTFSPRVRRATTSRTERIWPGGVIPYVIGGNFTGSQRAIF KQAMRHWEKHTCVTFIERTDEESFIVFSYRTCGCCSYVGRRGGGPQAISIGKNCDKFGIV AHELGHVVGFWHEHTRPDRDQHVTIIRENIQPGQEYNFLKMEAGEVSSLGETYDFDSIMH YARNTFSRGVFLDTILPRQDDNGVRPTIGQRVRLSQGDIAQARKLYKCPACGETLQDTTG NFSAPGFPNGYPSYSHCVWRISVTPGEKIVLNFTSMDLFKSRLCWYDYVEVRDGYWRKAP LLGRFCGDKIPEPLVSTDSRLWVEFRSSSNILGKGFFAAYEATCGGDMNKDAGQIQSPNY PDDYRPSKECVWRITVSEGFHVGLTFQAFEIERHDSCAYDYLEVRDGPTEESALIGHFCG YEKPEDVKSSSNRLWMKFVSDGSINKAGFAANFFKEVDECSWPDHGGCEHRCVNTLGSYK CACDPGYELAADKKMCEVACGGFITKLNGTITSPGWPKEYPTNKNCVWQVVAPAQYRISL QFEVFELEGNDVCKYDFVEVRSGLSPDAKLHGRFCGSETPEVITSQSNNMRVEFKSDNTV SKRGFRAHFFSDKDECAKDNGGCQHECVNTFGSYLCRCRNGYWLHENGHDCKEAGCAHKI SSVEGTLASPNWPDKYPSRRECTWNISSTAGHRVKLTFNEFEIEQHQECAYDHLEMYDGP DSLAPILGRFCGSKKPDPTVASGSSMFLRFYSDASVQRKGFQAVHSTECGGRLKAEVQTK ELYSHAQFGDNNYPSEARCDWVIVAEDGYGVELTFRTFEVEEEADCGYDYMEAYDGYDSS APRLGRFCGSGPLEEIYSAGDSLMIRFRTDDTINKKGFHARYTSTKFQDALHMKK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3082
Start on reference sequence
35
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
