DNASU Plasmid | RCOR1 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RCOR1

Gene Name: REST corepressor 1

Original Clone ID:

FLH267378.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00082601

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1446nts

Open reading frame: 1 to 1446

Reference Sequence Alignment

ATGGTGGAGAAGGGCCCCGAGGTCTCAGGGAAGCGGAGAGGGAGGAACAACGCGGCCGCC
TCCGCCTCCGCCGCCGCCGCCTCCGCCGCCGCCTCGGCCGCCTGCGCCTCGCCAGCCGCC
ACTGCCGCCTCGGGCGCCGCCGCCTCCTCAGCCTCGGCCGCCGCCGCCTCAGCCGCCGCC
GCCCCCAATAATGGCCAGAATAAAAGTTTGGCGGCGGCGGCGCCCAATGGCAACAGCAGC
AGCAACTCCTGGGAGGAAGGCAGCTCGGGCTCGTCCAGCGACGAGGAGCACGGTGGCGGT
GGCATGAGGGTCGGACCCCAGTACCAGGCGGTGGTGCCCGACTTCGACCCCGCCAAACTG
GCAAGACGCAGTCAAGAACGGGACAATCTTGGCATGTTGGTCTGGTCACCCAATCAAAAT
CTGTCAGAAGCAAAGTTGGATGAATACATTGCCATTGCCAAAGAAAAGCATGGGTACAAC
ATGGAACAGGCTCTTGGGATGCTCTTCTGGCATAAACATAATATCGAAAAGTCATTGGCT
GATTTGCCCAACTTTACCCCTTTCCCAGATGAGTGGACTGTGGAAGATAAAGTCTTATTT
GAGCAAGCCTTTAGTTTTCATGGGAAAACTTTTCATAGAATCCAACAAATGCTTCCAGAT
AAATCTATAGCAAGTCTGGTGAAATTTTACTATTCTTGGAAGAAGACGAGGACTAAAACT
AGTGTGATGGATCGCCATGCCCGGAAACAAAAACGGGAGCGGGAGGAGAGCGAGGATGAA
CTGGAAGAGGCAAATGGAAACAATCCCATTGACATTGAGGTTGATCAAAACAAGGAAAGC
AAAAAGGAGGTTCCCCCTACTGAGACAGTTCCTCAGGTCAAAAAAGAAAAACATAGCACA
CAAGCTAAAAATAGAGCAAAAAGGAAACCTCCAAAAGGAATGTTTCTTTCTCAAGAAGAT
GTGGAGGCTGTTTCTGCCAATGCCACTGCTGCTACCACGGTGCTGAGACAACTAGACATG
GAATTGGTTTCAGTCAAACGACAGATCCAGAATATTAAACAGACAAACAGTGCTCTCAAA
GAAAAACTTGATGGTGGAATAGAACCATATCGACTTCCAGAGGTCATTCAGAAATGTAAT
GCACGTTGGACTACAGAAGAGCAGCTTCTCGCCGTACAAGCCATCAGGAAATATGGCCGA
GATTTTCAGGCAATCTCAGACGTGATTGGGAACAAATCAGTGGTACAAGTGAAAAACTTT
TTTGTAAATTATCGACGCCGCTTCAACATAGATGAAGTTTTACAAGAATGGGAGGCAGAA
CATGGTAAAGAAGAGACCAATGGGCCCAGTAACCAGAAGCCTGTGAAGTCCCCAGATAAT
TCCATTAAGATGCCCGAAGAGGAAGACGAGGCTCCTGTTCTGGATGTCAGATATGCATCT
GCCTCC

Protein sequence predicted based on nucleotide sequence

MVEKGPEVSGKRRGRNNAAASASAAAASAAASAACASPAATAASGAAASSASAAAASAAA
APNNGQNKSLAAAAPNGNSSSNSWEEGSSGSSSDEEHGGGGMRVGPQYQAVVPDFDPAKL
ARRSQERDNLGMLVWSPNQNLSEAKLDEYIAIAKEKHGYNMEQALGMLFWHKHNIEKSLA
DLPNFTPFPDEWTVEDKVLFEQAFSFHGKTFHRIQQMLPDKSIASLVKFYYSWKKTRTKT
SVMDRHARKQKREREESEDELEEANGNNPIDIEVDQNKESKKEVPPTETVPQVKKEKHST
QAKNRAKRKPPKGMFLSQEDVEAVSANATAATTVLRQLDMELVSVKRQIQNIKQTNSALK
EKLDGGIEPYRLPEVIQKCNARWTTEEQLLAVQAIRKYGRDFQAISDVIGNKSVVQVKNF
FVNYRRRFNIDEVLQEWEAEHGKEETNGPSNQKPVKSPDNSIKMPEEEDEAPVLDVRYAS
AS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1483

Start on reference sequence 35

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

RCOR1

Symbol Nomenclature :

RCOR1

SYNONYM :

COREST; RCOR

Full Nomenclature :

REST corepressor 1

GENEID :

23186

GI :

109939873

GenBank Accession :

BC111590

HGNC :

17441

MIM :

607675

Vega :

OTTHUMG00000171778

Target GenBank:

BC111590

Reference Sequence Alignment

  

HsCD00082601      ---------ATGGTGGAGAAGGGCCCCGAGGTCTCAGGGAAGCGGAGAGGGAGGAACAAC
NM_015156.3       ATGCCGGCCATGGTGGAGAAGGGCCCCGAGGTCTCAGGGAAGCGGAGAGGGAGGAACAAC
                           ***************************************************

HsCD00082601      GCGGCCGCCTCCGCCTCCGCCGCCGCCGCCTCCGCCGCCGCCTCGGCCGCCTGCGCCTCG
NM_015156.3       GCGGCCGCCTCCGCCTCCGCCGCCGCCGCCTCCGCCGCCGCCTCGGCCGCCTGCGCCTCG
                  ************************************************************

HsCD00082601      CCAGCCGCCACTGCCGCCTCGGGCGCCGCCGCCTCCTCAGCCTCGGCCGCCGCCGCCTCA
NM_015156.3       CCAGCCGCCACTGCCGCCTCGGGCGCCGCCGCCTCCTCAGCCTCGGCCGCCGCCGCCTCA
                  ************************************************************

HsCD00082601      GCCGCCGCCGCCCCCAATAATGGCCAGAATAAAAGTTTGGCGGCGGCGGCGCCCAATGGC
NM_015156.3       GCCGCCGCCGCCCCCAATAATGGCCAGAATAAAAGTTTGGCGGCGGCGGCGCCCAATGGC
                  ************************************************************

HsCD00082601      AACAGCAGCAGCAACTCCTGGGAGGAAGGCAGCTCGGGCTCGTCCAGCGACGAGGAGCAC
NM_015156.3       AACAGCAGCAGCAACTCCTGGGAGGAAGGCAGCTCGGGCTCGTCCAGCGACGAGGAGCAC
                  ************************************************************

HsCD00082601      GGTGGCGGTGGCATGAGGGTCGGACCCCAGTACCAGGCGGTGGTGCCCGACTTCGACCCC
NM_015156.3       GGTGGCGGTGGCATGAGGGTCGGACCCCAGTACCAGGCGGTGGTGCCCGACTTCGACCCC
                  ************************************************************

HsCD00082601      GCCAAACTGGCAAGACGCAGTCAAGAACGGGACAATCTTGGCATGTTGGTCTGGTCACCC
NM_015156.3       GCCAAACTGGCAAGACGCAGTCAAGAACGGGACAATCTTGGCATGTTGGTCTGGTCACCC
                  ************************************************************

HsCD00082601      AATCAAAATCTGTCAGAAGCAAAGTTGGATGAATACATTGCCATTGCCAAAGAAAAGCAT
NM_015156.3       AATCAAAATCTGTCAGAAGCAAAGTTGGATGAATACATTGCCATTGCCAAAGAAAAGCAT
                  ************************************************************

HsCD00082601      GGGTACAACATGGAACAGGCTCTTGGGATGCTCTTCTGGCATAAACATAATATCGAAAAG
NM_015156.3       GGGTACAACATGGAACAGGCTCTTGGGATGCTCTTCTGGCATAAACATAATATCGAAAAG
                  ************************************************************

HsCD00082601      TCATTGGCTGATTTGCCCAACTTTACCCCTTTCCCAGATGAGTGGACTGTGGAAGATAAA
NM_015156.3       TCATTGGCTGATTTGCCCAACTTTACCCCTTTCCCAGATGAGTGGACTGTGGAAGATAAA
                  ************************************************************

HsCD00082601      GTCTTATTTGAGCAAGCCTTTAGTTTTCATGGGAAAACTTTTCATAGAATCCAACAAATG
NM_015156.3       GTCTTATTTGAGCAAGCCTTTAGTTTTCATGGGAAAACTTTTCATAGAATCCAACAAATG
                  ************************************************************

HsCD00082601      CTTCCAGATAAATCTATAGCAAGTCTGGTGAAATTTTACTATTCTTGGAAGAAGACGAGG
NM_015156.3       CTTCCAGATAAATCTATAGCAAGTCTGGTGAAATTTTACTATTCTTGGAAGAAGACGAGG
                  ************************************************************

HsCD00082601      ACTAAAACTAGTGTGATGGATCGCCATGCCCGGAAACAAAAACGGGAGCGGGAGGAGAGC
NM_015156.3       ACTAAAACTAGTGTGATGGATCGCCATGCCCGGAAACAAAAACGGGAGCGGGAGGAGAGC
                  ************************************************************

HsCD00082601      GAGGATGAACTGGAAGAGGCAAATGGAAACAATCCCATTGACATTGAGGTTGATCAAAAC
NM_015156.3       GAGGATGAACTGGAAGAGGCAAATGGAAACAATCCCATTGACATTGAGGTTGATCAAAAC
                  ************************************************************

HsCD00082601      AAGGAAAGCAAAAAGGAGGTTCCCCCTACTGAGACAGTTCCTCAGGTCAAAAAAGAAAAA
NM_015156.3       AAGGAAAGCAAAAAGGAGGTTCCCCCTACTGAGACAGTTCCTCAGGTCAAAAAAGAAAAA
                  ************************************************************

HsCD00082601      CATAGCACACAAGCTAAAAATAGAGCAAAAAGGAAACCTCCAAAAGGAATGTTTCTTTCT
NM_015156.3       CATAGCACACAAGCTAAAAATAGAGCAAAAAGGAAACCTCCAAAAGGAATGTTTCTTTCT
                  ************************************************************

HsCD00082601      CAAGAAGATGTGGAGGCTGTTTCTGCCAATGCCACTGCTGCTACCACGGTGCTGAGACAA
NM_015156.3       CAAGAAGATGTGGAGGCTGTTTCTGCCAATGCCACTGCTGCTACCACGGTGCTGAGACAA
                  ************************************************************

HsCD00082601      CTAGACATGGAATTGGTTTCAGTCAAACGACAGATCCAGAATATTAAACAGACAAACAGT
NM_015156.3       CTAGACATGGAATTGGTTTCAGTCAAACGACAGATCCAGAATATTAAACAGACAAACAGT
                  ************************************************************

HsCD00082601      GCTCTCAAAGAAAAACTTGATGGTGGAATAGAACCATATCGACTTCCAGAGGTCATTCAG
NM_015156.3       GCTCTCAAAGAAAAACTTGATGGTGGAATAGAACCATATCGACTTCCAGAGGTCATTCAG
                  ************************************************************

HsCD00082601      AAATGTAATGCACGTTGGACTACAGAAGAGCAGCTTCTCGCCGTACAAGCCATCAGGAAA
NM_015156.3       AAATGTAATGCACGTTGGACTACAGAAGAGCAGCTTCTCGCCGTACAAGCCATCAGGAAA
                  ************************************************************

HsCD00082601      TATGGCCGAGATTTTCAGGCAATCTCAGACGTGATTGGGAACAAATCAGTGGTACAAGTG
NM_015156.3       TATGGCCGAGATTTTCAGGCAATCTCAGACGTGATTGGGAACAAATCAGTGGTACAAGTG
                  ************************************************************

HsCD00082601      AAAAACTTTTTTGTAAATTATCGACGCCGCTTCAACATAGATGAAGTTTTACAAGAATGG
NM_015156.3       AAAAACTTTTTTGTAAATTATCGACGCCGCTTCAACATAGATGAAGTTTTACAAGAATGG
                  ************************************************************

HsCD00082601      GAGGCAGAACATGGTAAAGAAGAGACCAATGGGCCCAGTAACCAGAAGCCTGTGAAGTCC
NM_015156.3       GAGGCAGAACATGGTAAAGAAGAGACCAATGGGCCCAGTAACCAGAAGCCTGTGAAGTCC
                  ************************************************************

HsCD00082601      CCAGATAATTCCATTAAGATGCCCGAAGAGGAAGACGAGGCTCCTGTTCTGGATGTCAGA
NM_015156.3       CCAGATAATTCCATTAAGATGCCCGAAGAGGAAGACGAGGCTCCTGTTCTGGATGTCAGA
                  ************************************************************

HsCD00082601      TATGCATCTGCCTCCTGA
NM_015156.3       TATGCATCTGCCTCCTGA
                  ******************