DNASU Plasmid | RNF39 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RNF39

Gene Name: ring finger protein 39

Original Clone ID:

FLH267382.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00082605

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1263nts

Open reading frame: 1 to 1263

Reference Sequence Alignment

ATGTGGTGGAGAGATTTGACGAGACTGAGACTCTGGTTGAAGAGAGAGGCAATCCCAGGA
GAGGGGCGGAAAGCGGCAAAAGTTAATGCGGGAGTCGGAGAGAAGGGCATCTACACAGCA
AGCAGCAGGGGCGGCCCGCCATCTGCGCGCTCGAAGGCGGTCACGGTGGTCGCGGAAGGG
GCGGCGTCCAGATCCTGGCTTTCCATGGATGCGCCCGAGCTGGGCCCGGGGCTGGTGGAG
CGTCTGGAGCAGCTGGCGACGTGTCCTCTGTGCGGGGGCTCCTTCGAGGACCCGGTGCTT
CTGGCGTGCGAGCACAGCTTCTGCCGCGCGTGTCTGGCCCGCCGCTGGGGGACTCCGCCG
GCGACCGGCACCGAGGCTTCCCCCACCGCCTGTCCCTGCTGCGGCCTGCCGTGTCCCCGC
CGCAGCCTGAGGTCTAATGTGCGGCTGGCGGTGGAGGTGCGAATCAGCCGCGAGCTGCGA
GAGAAGCTGGCTGAGCCTGGGGCCCGTGCGGGGAGACGCCGAGGGGGGCGCATCCCCACC
ATGGGCTGCCTGGACCTGCCCGGAGAGGATATGAGGAAGACATGGAGACGATTTGAAGTC
CCAACATCCAAGTCATCTAATTCAGAGGATGATCTCCCTGAAGATTATCCAGTGGTCAAA
AAAATGCTTCATAGACTGACAGCCGACCTGACCCTGGACCCTGGGACCGCACACCGCCGC
CTGCTCATCTCCGCCGACCGCCGCAGCGTACAACTGGCCCCACCAGGGACGCCCGCGCCC
CCTGACGGCCCCAAGCGCTTCGATCAGCTCCCAGCTGTGCTGGGTGCGCAGGGCTTCGGG
GCCGGCCGCCACTGCTGGGAGGTGGAGACTGCGGACGCCGCCTCCTGCAGAGACTCTTCT
GGGGAGGATGCGGACGACGAGGAGAGCCACTATGCAGTGGGCGCGGCCGGGGAATCAGTG
CAACGCAAGGGCTGCGTAAGGCTGTGCCCTGCGGGGGCCGTGTGGGCCGTGGAGGGCCGC
GGCGGCCGCCTGTGGGCCCTCACGGCACCCGAACCCACCCTGCTGGGCGGTGTTGAGCCC
CCGCCGCGGCGCATTCGCGTGGACCTGGACTGGGAGCGGGGCCGCGTGGCCTTCTACGAC
GGCCGCTCACTCGACCTGCTTTACGCCTTCCAGGCGCCTGGCCCCCTGGGGGAGCGCATC
TTCCCGCTGTTCTGCACCTGCGACCCTCGTGCTCCGCTCCGCATTGTACCAGCGGAAAGC
TGA

Protein sequence predicted based on nucleotide sequence

MWWRDLTRLRLWLKREAIPGEGRKAAKVNAGVGEKGIYTASSRGGPPSARSKAVTVVAEG
AASRSWLSMDAPELGPGLVERLEQLATCPLCGGSFEDPVLLACEHSFCRACLARRWGTPP
ATGTEASPTACPCCGLPCPRRSLRSNVRLAVEVRISRELREKLAEPGARAGRRRGGRIPT
MGCLDLPGEDMRKTWRRFEVPTSKSSNSEDDLPEDYPVVKKMLHRLTADLTLDPGTAHRR
LLISADRRSVQLAPPGTPAPPDGPKRFDQLPAVLGAQGFGAGRHCWEVETADAASCRDSS
GEDADDEESHYAVGAAGESVQRKGCVRLCPAGAVWAVEGRGGRLWALTAPEPTLLGGVEP
PPRRIRVDLDWERGRVAFYDGRSLDLLYAFQAPGPLGERIFPLFCTCDPRAPLRIVPAES
*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1297

Start on reference sequence 35

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

RNF39

Symbol Nomenclature :

RNF39

SYNONYM :

HZF; HZFW; LIRF

Designation :

LTP (long-term potentiation) induced RING finger protein|RING finger protein 39|protein HZFw

Full Nomenclature :

ring finger protein 39

GENEID :

80352

Locus Tag :

DADB-60O11.8

GI :

109939744

GenBank Accession :

BC111601

HGNC :

18064

MIM :

607524

Vega :

OTTHUMG00000031288

Target GenBank:

BC111601

Reference Sequence Alignment

  

HsCD00082605      ATGTGGTGGAGAGATTTGACGAGACTGAGACTCTGGTTGAAGAGAGAGGCAATCCCAGGA
NM_025236.3       ATGTGGTGGAGAGATTTGACGAGACTGAGACTCTGGTTGAAGAGAGAGGCAATCCCAGGA
                  ************************************************************

HsCD00082605      GAGGGGCGGAAAGCGGCAAAAGTTAATGCGGGAGTCGGAGAGAAGGGCATCTACACAGCA
NM_025236.3       GAGGGGCGGAAAGCGGCAAAAGTTAATGCGGGAGTCGGAGAGAAGGGCATCTACACAGCA
                  ************************************************************

HsCD00082605      AGCAGCAGGGGCGGCCCGCCATCTGCGCGCTCGAAGGCGGTCACGGTGGTCGCGGAAGGG
NM_025236.3       AGCAGCAGGGGCGGCCCGCCATCTGCGCGCTCGAAGGCGGTCACGGTGGTCGCGGAAGGG
                  ************************************************************

HsCD00082605      GCGGCGTCCAGATCCTGGCTTTCCATGGATGCGCCCGAGCTGGGCCCGGGGCTGGTGGAG
NM_025236.3       GCGGCGTCCAGATCCTGGCTTTCCATGGATGCGCCCGAGCTGGGCCCGGGGCTGGTGGAG
                  ************************************************************

HsCD00082605      CGTCTGGAGCAGCTGGCGACGTGTCCTCTGTGCGGGGGCTCCTTCGAGGACCCGGTGCTT
NM_025236.3       CGTCTGGAGCAGCTGGCGACGTGTCCTCTGTGCGGGGGCTCCTTCGAGGACCCGGTGCTT
                  ************************************************************

HsCD00082605      CTGGCGTGCGAGCACAGCTTCTGCCGCGCGTGTCTGGCCCGCCGCTGGGGGACTCCGCCG
NM_025236.3       CTGGCGTGCGAGCACAGCTTCTGCCGCGCGTGTCTGGCCCGCCGCTGGGGGACTCCGCCG
                  ************************************************************

HsCD00082605      GCGACCGGCACCGAGGCTTCCCCCACCGCCTGTCCCTGCTGCGGCCTGCCGTGTCCCCGC
NM_025236.3       GCGACCGGCACCGAGGCTTCCCCCACCGCCTGTCCCTGCTGCGGCCTGCCGTGTCCCCGC
                  ************************************************************

HsCD00082605      CGCAGCCTGAGGTCTAATGTGCGGCTGGCGGTGGAGGTGCGAATCAGCCGCGAGCTGCGA
NM_025236.3       CGCAGCCTGAGGTCTAATGTGCGGCTGGCGGTGGAGGTGCGAATCAGCCGCGAGCTGCGA
                  ************************************************************

HsCD00082605      GAGAAGCTGGCTGAGCCTGGGGCCCGTGCGGGGAGACGCCGAGGGGGGCGCATCCCCACC
NM_025236.3       GAGAAGCTGGCTGAGCCTGGGGCCCGTGCGGGGAGACGCCGAGGGGGGCGCATCCCCACC
                  ************************************************************

HsCD00082605      ATGGGCTGCCTGGACCTGCCCGGAGAGGATATGAGGAAGACATGGAGACGATTTGAAGTC
NM_025236.3       ATGGGCTGCCTGGACCTGCCCGGAGAGGATATGAGGAAGACATGGAGACGATTTGAAGTC
                  ************************************************************

HsCD00082605      CCAACATCCAAGTCATCTAATTCAGAGGATGATCTCCCTGAAGATTATCCAGTGGTCAAA
NM_025236.3       CCAACATCCAAGTCATCTAATTCAGAGGATGATCTCCCTGAAGATTATCCAGTGGTCAAA
                  ************************************************************

HsCD00082605      AAAATGCTTCATAGACTGACAGCCGACCTGACCCTGGACCCTGGGACCGCACACCGCCGC
NM_025236.3       AAAATGCTTCATAGACTGACAGCCGACCTGACCCTGGACCCTGGGACCGCACACCGCCGC
                  ************************************************************

HsCD00082605      CTGCTCATCTCCGCCGACCGCCGCAGCGTACAACTGGCCCCACCAGGGACGCCCGCGCCC
NM_025236.3       CTGCTCATCTCCGCCGACCGCCGCAGCGTACAACTGGCCCCACCAGGGACGCCCGCGCCC
                  ************************************************************

HsCD00082605      CCTGACGGCCCCAAGCGCTTCGATCAGCTCCCAGCTGTGCTGGGTGCGCAGGGCTTCGGG
NM_025236.3       CCTGACGGCCCCAAGCGCTTCGATCAGCTCCCAGCTGTGCTGGGTGCGCAGGGCTTCGGG
                  ************************************************************

HsCD00082605      GCCGGCCGCCACTGCTGGGAGGTGGAGACTGCGGACGCCGCCTCCTGCAGAGACTCTTCT
NM_025236.3       GCCGGCCGCCACTGCTGGGAGGTGGAGACTGCGGACGCCGCCTCCTGCAGAGACTCTTCT
                  ************************************************************

HsCD00082605      GGGGAGGATGCGGACGACGAGGAGAGCCACTATGCAGTGGGCGCGGCCGGGGAATCAGTG
NM_025236.3       GGGGAGGATGCGGACGACGAGGAGAGCCACTATGCAGTGGGCGCGGCCGGGGAATCAGTG
                  ************************************************************

HsCD00082605      CAACGCAAGGGCTGCGTAAGGCTGTGCCCTGCGGGGGCCGTGTGGGCCGTGGAGGGCCGC
NM_025236.3       CAACGCAAGGGCTGCGTAAGGCTGTGCCCTGCGGGGGCCGTGTGGGCCGTGGAGGGCCGC
                  ************************************************************

HsCD00082605      GGCGGCCGCCTGTGGGCCCTCACGGCACCCGAACCCACCCTGCTGGGCGGTGTTGAGCCC
NM_025236.3       GGCGGCCGCCTGTGGGCCCTCACGGCACCCGAACCCACCCTGCTGGGCGGTGTTGAGCCC
                  ************************************************************

HsCD00082605      CCGCCGCGGCGCATTCGCGTGGACCTGGACTGGGAGCGGGGCCGCGTGGCCTTCTACGAC
NM_025236.3       CCGCCGCGGCGCATTCGCGTGGACCTGGACTGGGAGCGGGGCCGCGTGGCCTTCTACGAC
                  ************************************************************

HsCD00082605      GGCCGCTCACTCGACCTGCTTTACGCCTTCCAGGCGCCTGGCCCCCTGGGGGAGCGCATC
NM_025236.3       GGCCGCTCACTCGACCTGCTTTACGCCTTCCAGGCGCCTGGCCCCCTGGGGGAGCGCATC
                  ************************************************************

HsCD00082605      TTCCCGCTGTTCTGCACCTGCGACCCTCGTGCTCCGCTCCGCATTGTACCAGCGGAAAGC
NM_025236.3       TTCCCGCTGTTCTGCACCTGCGACCCTCGTGCTCCGCTCCGCATTGTACCAGCGGAAAGC
                  ************************************************************

HsCD00082605      TGA
NM_025236.3       TGA
                  ***