Gene:
Gene Symbol:
NCAN
Gene Name:
neurocan
Original Clone ID:
FLH267449.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082672
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGGGCCCCGTTTGTCTGGGCCTTGGGCCTTTTGATGCTGCAGATGCTGCTCTTTGTG
GCTGGGGAACAGGGCACACAGGATATCACCGATGCCAGCGAAAGGGGGCTCCACATGCAG
AAGCTGGGGTCTGGGTCAGTGCAGGCTGCGCTGGCGGAGCTGGTGGCCCTGCCCTGTCTC
TTTACCCTGCAGCCACGGCCAAGCGCAGCCCGAGATGCCCCTCGGATAAAGTGGACCAAG
GTGCGGACTGCGTCGGGCCAGCGACAGGACTTGCCCATCCTGGTGGCCAAGGACAATGTC
GTGAGGGTGGCCAAAAGCTGGCAGGGACGAGTGTCACTGCCTTCCTACCCCCGGCGCCGA
GCCAACGCCACGCTACTTCTGGGGCCACTGAGGGCCAGTGACTCTGGGCTGTACCGCTGC
CAGGTGGTGAGGGGCATCGAGGATGAGCAGGACCTGGTGCCCTTGGAGGTGACAGGTGTT
GTGTTCCACTACCGATCAGCCCGGGACCGCTATGCACTGACCTTCGCTGAGGCCCAGGAG
GCCTGCCGTCTCAGCTCAGCCATCATTGCAGCCCCTCGGCATCTACAGGCTGCCTTTGAG
GATGGCTTTGACAACTGTGATGCTGGCTGGCTCTCTGACCGCACTGTTCGGTATCCTATC
ACCCAGTCCCGTCCTGGTTGCTATGGCGACCGTAGCAGCCTTCCAGGGGTTCGGAGCTAT
GGGAGGCGCAACCCACAGGAACTCTACGATGTGTATTGCTTTGCCCGGGAGCTGGGGGGC
GAGGTCTTCTACGTGGGCCCGGCCCGCCGCCTGACACTGGCCGGCGCGCGTGCACAGTGC
CGCCGCCAGGGTGCCGCGCTGGCCTCGGTGGGACAGCTGCACCTGGCCTGGCATGAGGGC
CTGGACCAGTGCGACCCGGGCTGGCTGGCCGACGGCAGCGTGCGCTACCCGATCCAGACG
CCGCGCCGGCGCTGCGGGGGCCCAGCCCCGGGCGTGCGCACCGTCTACCGCTTCGCTAAC
CGGACCGGCTTCCCCTCACCCGCCGAGCGCTTCGACGCCTACTGCTTCCGAGCTCATCAC
CCCACGTCACAACATGGAGACCTAGAGACCCCATCCTCTGGGGATGAGGGGGAGATTCTG
TCAGCAGAGGGGCCCCCAGTTAGAGAACTGGAGCCCACCCTGGAGGAGGAAGAGGTGGTC
ACCCCTGACTTCCAGGAGCCTCTGGTGTCCAGTGGGGAAGAAGAAACCCTGATTTTGGAG
GAGAAGCAGGAGTCTCAACAGACCCTCAGCCCTACCCCTGGGGACCCCATGCTGGCCTCA
TGGCCCACTGGGGAAGTGTGGCTAAGCACGGTGGCCCCCAGCCCTAGCGACATGGGGGCA
GGCACTGCAGCAAGTTCACACACGGAGGTGGCCCCAACTGACCCTATGCCTAGGAGAAGG
GGGCGCTTCAAAGGGTTGAATGGGCGCTACTTCCAGCAGCAGGAACCGGAGCCGGGGCTG
CAAGGGGGGATGGAGGCCAGCGCCCAGCCCCCCACCTCAGAGGCTGCAGTGAACCAAATG
GAGCCTCCGTTGGCCATGGCAGTCACAGAGATGTTGGGCAGTGGCCAGAGCCGGAGCCCC
TGGGCTGATCTGACCAATGAGGTGGATATGCCTGGAGCTGGTTCTGCTGGTGGCAAGAGC
TCCCCAGAGCCCTGGCTGTGGCCCCCTACCATGGTCCCACCCAGCATCTCAGGCCACAGC
AGGGCCCCTGTCCTGGAGCTAGAGAAAGCCGAGGGCCCCAGTGCCAGGCCAGCCACCCCA
GACCTGTTTTGGTCCCCCTTGGAGGCCACTGTCTCAGCTCCCAGCCCTGCCCCCTGGGAG
GCATTCCCTGTGGCCACCTCCCCAGATCTCCCTATGATGGCCATGCTGCGTGGTCCCAAA
GAGTGGATGCTACCACACCCCACCCCCATCTCCACCGAGGCCAATAGAGTTGAGGCACAT
GGTGAGGCCACCGCCACGGCTCCACCCTCCCCTGCTGCAGAGACCAAGGTGTATTCCCTG
CCTCTCTCTTTGACCCCAACAGGACAGGGTGGAGAGGCCATGCCCACAACACCTGAGTCC
CCCAGGGCAGACTTCAGAGAAACTGGGGAGACCAGCCCTGCTCAGGTCAACAAAGCTGAG
CACTCCAGCTCCAGCCCATGGCCTTCTGTAAACAGGAATGTGGCTGTAGGTTTTGTCCCC
ACTGAGACTGCCACTGAGCCAACGGGCCTCAGGGGTATCCCGGGGTCTGAGTCTGGGGTC
TTCGACACAGCAGAAAGCCCCACTTCTGGCTTGCAGGCCACTGTAGATGAGGTGCAGGAC
CCCTGGCCCTCAGTGTACAGCAAAGGGCTGGATGCAAGTTCCCCATCTGCCCCCCTGGGG
AGCCCTGGAGTCTTCTTGGTACCCAAAGTCACCCCAAATTTGGAGCCTTGGGTTGCTACA
GATGAAGGACCCACTGTGAATCCCATGGATTCCACAGTCACGCCGGCCCCCAGTGATGCT
AGTGGAATTTGGGAACCTGGATCCCAGGTGTTTGAAGAAGCCGAAAGCACCACCTTGAGC
CCTCAGGTGGCCCTGGATACAAGCATTGTGACGCCCCTCACGACCCTGGAGCAGGGGGAC
AAGGTTGGAGTTCCAGCCATGTCTACACTGGGCTCCTCAAGCTCCCAACCCCACCCAGAG
CCAGAGGATCAGGTGGAGACCCAGGGAACATCAGGAGCTTCAGTGCCTCCGCATCAGAGC
AGTCCCCTAGGGAAACCGGCTGTTCCTCCTGGGACACCGACTGCAGCCAGTGTGGGCGAG
TCTGCCTCAGTTTCCTCAGGGGAGCCTACGGTACCGTGGGACCCCTCCAGCACCCTGCTG
CCTGTCACCCTGGGCATAGAGGACTTCGAACTGGAGGTCCTGGCAGGGAGCCCGGGTGTA
GAGAGCTTCTGGGAGGAGGTGGCAAGTGGAGAGGAGCCAGCCCTGCCAGGGACCCCTATG
AATGCAGGTGCGGAGGAGGTGCACTCAGATCCCTGTGAGAACAACCCTTGTCTTCATGGA
GGGACATGTAATGCCAATGGCACCATGTATGGCTGTAGCTGTGATCAGGGCTTCGCCGGG
GAGAACTGTGAGATTGACATTGATGACTGCCTCTGCAGCCCCTGTGAGAATGGAGGCACC
TGTATTGATGAGGTCAATGGCTTTGTCTGCCTTTGCCTCCCCAGCTATGGGGGCAGCTTT
TGTGAGAAAGACACCGAGGGCTGTGACCGCGGCTGGCATAAGTTCCAGGGCCACTGTTAC
CGCTATTTTGCCCACCGGAGGGCATGGGAAGATGCCGAGAAGGACTGCCGCCGCCGCTCC
GGCCACCTGACCAGCGTCCACTCACCGGAGGAACACAGCTTCATTAATAGCTTTGGGCAT
GAAAACACGTGGATCGGCCTGAACGACAGGATCGTGGAGAGAGATTTCCAGTGGACGGAC
AACACCGGGCTGCAATTTGAGAACTGGCGAGAGAACCAGCCGGACAATTTCTTCGCGGGT
GGCGAGGACTGTGTGGTGATGGTGGCGCATGAAAGCGGGCGCTGGAACGATGTCCCCTGC
AACTACAACCTACCCTATGTCTGCAAGAAGGGCACAGTGCTCTGTGGTCCCCCTCCGGCA
GTGGAGAATGCCTCACTCATCGGTGCCCGCAAGGCCAAGTACAATGTCCATGCCACTGTA
AGGTACCAGTGCAATGAAGGATTTGCCCAGCACCATGTGGCCACCATTCGATGCCGGAGC
AATGGCAAGTGGGACAGGCCCCAAATTGTCTGCACCAAACCCAGACGTTCACATCGGATG
CGGCGACACCACCACCACCACCAACACCACCACCAGCATCACCACCACAAATCCCGCAAG
GAGCGCAGAAAACACAAGAAACACCCAACGGAGGACTGGGAGAAGGACGAAGGGAATTTC
TGC
Protein sequence predicted based on nucleotide sequence
MGAPFVWALGLLMLQMLLFVAGEQGTQDITDASERGLHMQKLGSGSVQAALAELVALPCL FTLQPRPSAARDAPRIKWTKVRTASGQRQDLPILVAKDNVVRVAKSWQGRVSLPSYPRRR ANATLLLGPLRASDSGLYRCQVVRGIEDEQDLVPLEVTGVVFHYRSARDRYALTFAEAQE ACRLSSAIIAAPRHLQAAFEDGFDNCDAGWLSDRTVRYPITQSRPGCYGDRSSLPGVRSY GRRNPQELYDVYCFARELGGEVFYVGPARRLTLAGARAQCRRQGAALASVGQLHLAWHEG LDQCDPGWLADGSVRYPIQTPRRRCGGPAPGVRTVYRFANRTGFPSPAERFDAYCFRAHH PTSQHGDLETPSSGDEGEILSAEGPPVRELEPTLEEEEVVTPDFQEPLVSSGEEETLILE EKQESQQTLSPTPGDPMLASWPTGEVWLSTVAPSPSDMGAGTAASSHTEVAPTDPMPRRR GRFKGLNGRYFQQQEPEPGLQGGMEASAQPPTSEAAVNQMEPPLAMAVTEMLGSGQSRSP WADLTNEVDMPGAGSAGGKSSPEPWLWPPTMVPPSISGHSRAPVLELEKAEGPSARPATP DLFWSPLEATVSAPSPAPWEAFPVATSPDLPMMAMLRGPKEWMLPHPTPISTEANRVEAH GEATATAPPSPAAETKVYSLPLSLTPTGQGGEAMPTTPESPRADFRETGETSPAQVNKAE HSSSSPWPSVNRNVAVGFVPTETATEPTGLRGIPGSESGVFDTAESPTSGLQATVDEVQD PWPSVYSKGLDASSPSAPLGSPGVFLVPKVTPNLEPWVATDEGPTVNPMDSTVTPAPSDA SGIWEPGSQVFEEAESTTLSPQVALDTSIVTPLTTLEQGDKVGVPAMSTLGSSSSQPHPE PEDQVETQGTSGASVPPHQSSPLGKPAVPPGTPTAASVGESASVSSGEPTVPWDPSSTLL PVTLGIEDFELEVLAGSPGVESFWEEVASGEEPALPGTPMNAGAEEVHSDPCENNPCLHG GTCNANGTMYGCSCDQGFAGENCEIDIDDCLCSPCENGGTCIDEVNGFVCLCLPSYGGSF CEKDTEGCDRGWHKFQGHCYRYFAHRRAWEDAEKDCRRRSGHLTSVHSPEEHSFINSFGH ENTWIGLNDRIVERDFQWTDNTGLQFENWRENQPDNFFAGGEDCVVMVAHESGRWNDVPC NYNLPYVCKKGTVLCGPPPAVENASLIGARKAKYNVHATVRYQCNEGFAQHHVATIRCRS NGKWDRPQIVCTKPRRSHRMRRHHHHHQHHHQHHHHKSRKERRKHKKHPTEDWEKDEGNF C
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
35
End on reference sequence
3997
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
