Gene:
Gene Symbol:
TSHZ1
Gene Name:
teashirt zinc finger homeobox 1
Original Clone ID:
FLH269539.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082765
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTGCAATGAAGAGACGGAGATCAAAGAGGCGCAGAGCTACCAGAACTCCCCAGTCAGC
TCTGCGACTAACCAGGACGCCGGCTACGGGTCGCCCTTCAGTGAGAGCAGCGACCAGCTA
GCCCATTTCAAAGGCTCTTCCTCTCGAGAAGAGAAGGAGGATCCGCAGTGTCCCGACAGC
GTCTCGTACCCCCAGGACAGCCTGGCACAGATCAAAGCTGTGTATGCAAACTTGTTCTCC
GAGTCCTGCTGGTCCAGCTTAGCTCTGGATTTAAAGAAGTCGGGTTCCACCACCAGCACC
AACGATGCCAGCCAGAAGGAGAGCTCCGCCCCCACCCCCACACCCCCCACCTGCCCCGTC
AGCACCACTGGCCCCACCACGAGCACGCCCAGCACCAGCTGCAGCTCCAGCACCAGCCAC
AGCAGTACCACCAGTACCAGCAGCAGCTCCGGGTACGACTGGCACCAGGCTGCACTGGCC
AAGACGCTGCAGCAGACGTCCTCGTATGGGCTGCTTCCTGAGCCCAGCCTGTTCAGCACC
GTGCAGCTCTACCGCCAGAACAACAAGCTCTACGGCTCCGTCTTCACGGGCGCCAGCAAG
TTCCGGTGCAAAGACTGCAGTGCCGCGTACGACACGCTGGTGGAACTGACGGTGCACATG
AACGAGACAGGCCACTACCGTGACGACAACAGGGACAAGGACTCCGAGAAGACCAAGAGG
TGGTCCAAGCCCAGGAAGCGCTCCCTGATGGAGATGGAGGGGAAGGAGGATGCCCAGAAG
GTGCTGAAGTGCATGTACTGTGGACACTCCTTTGAGTCCTTGCAGGACCTCAGCGTCCAC
ATGATCAAAACCAAGCATTACCAGAAAGTGCCTCTGAAGGAGCCAGTGCCAGCCATCACC
AAACTGGTCCCCTCCACCAAAAAGCGGGCGCTTCAGGACCTGGCGCCCCCCTGCTCCCCT
GAGCCAGCAGGAATGGCCGCAGAGGTGGCCCTGAGTGAGTCAGCCAAGGATCAGAAAGCA
GCGAACCCGTACGTCACGCCCAATAACCGCTATGGCTACCAGAATGGCGCCAGCTACACC
TGGCAGTTTGAGGCCCGCAAGGCGCAGATCCTCAAGTGCATGGAGTGTGGCAGCTCCCAC
GACACGCTGCAGCAGCTCACCGCCCACATGATGGTCACCGGGCACTTCCTGAAAGTGACC
ACCTCGGCTTCTAAGAAGGGCAAGCAGTTGGTGCTGGACCCTGTGGTGGAAGAGAAGATC
CAGTCCATCCCACTACCGCCCACCACCCACACGCGGCTGCCGGCCTCCAGCATCAAAAAG
CAGCCCGACTCTCCCGCGGGGTCCACGACTTCTGAAGAAAAGAAAGAGCCAGAGAAGGAG
AAGCCGCCTGTGGCTGGCGACGCGGAGAAGATCAAGGAGGAGAGTGAGGACAGCTTGGAG
AAATTTGAGCCCAGCACCCTGTACCCGTACCTGCGTGAGGAGGACCTGGACGACAGCCCC
AAGGGAGGGCTGGACATTCTCAAGTCCCTGGAGAATACCGTCTCCACGGCCATTAGCAAA
GCTCAGAATGGTGCGCCCTCATGGGGTGGCTACCCCAGCATCCATGCAGCCTACCAGCTC
CCGGGCACCGTGAAGCCACTGCCGGCGGCCGTGCAGAGCGTGCAGGTGCAGCCGTCCTAT
GCTGGCGGCGTGAAGTCGCTGTCTTCCGCCGAGCACAACGCCCTCCTGCACTCCCCAGGG
AGCCTCACGCCCCCACCGCACAAGAGCAACGTGTCTGCCATGGAGGAGCTGGTGGAGAAG
GTCACGGGCAAGGTCAACATCAAGAAGGAGGAGAGACCCCCTGAGAAGGAGAAGAGCTCC
CTGGCCAAGGCTGCGTCCCCCATAGCAAAAGAGAATAAAGATTTCCCGAAAACGGAGGAA
GTCAGCGGCAAACCACAGAAGAAGGGCCCTGAGGCCGAGACTGGGAAGGCCAAAAAGGAG
GGACCGCTGGACGTTCACACCCCAAATGGCACAGAGCCTCTCAAAGCAAAGGTCACCAAC
GGCTGTAACAACCTGGGGATCATCATGGACCACTCACCGGAGCCTTCCTTCATCAACCCG
CTGAGCGCTTTGCAGTCCATCATGAACACCCACCTGGGCAAGGTGTCCAAGCCCGTGAGT
CCCTCGCTGGACCCGCTGGCGATGCTGTACAAGATCAGCAACAGCATGCTGGACAAGCCG
GTGTACCCCGCCACCCCTGTGAAGCAGGCCGATGCCATCGACCGCTACTATTATGAAAAC
AGCGACCAGCCCATTGACTTAACCAAGTCCAAGAACAAGCCGCTGGTGTCCAGCGTGGCT
GATTCGGTGGCATCACCTCTGCGGGAGAGCGCACTCATGGACATCTCCGACATGGTGAAA
AACCTCACAGGCCGCCTGACGCCCAAGTCCTCCACGCCCTCCACAGTTTCAGAGAAGTCC
GATGCTGATGGCAGCAGCTTTGAGGAGGCGTTGGACGAGCTGTCACCGGTCCACAAGAGG
AAGGGCCGGCAGTCCAACTGGAACCCGCAGCACCTTCTCATCCTGCAGGCCCAGTTCGCC
TCGAGCTTGCGGGAGACCACAGAGGGCAAGTACATCATGTCGGACTTGGGCCCGCAGGAG
AGGGTGCACATCTCGAAGTTTACTGGGCTCTCCATGACCACCATCAGCCACTGGCTGGCC
AATGTGAAGTACCAGTTGAGGAGGACAGGGGGAACGAAATTCCTAAAGAACCTGGACACA
GGGCATCCTGTTTTCTTTTGCAACGATTGTGCCTCTCAGTTCAGAACTGCTTCTACATAC
ATAAGTCATTTGGAGACACACTTGGGCTTCAGCCTGAAGGATCTCTCCAAGCTGCCACTC
AATCAGATTCAAGAACAGCAGAATGTTTCGAAAGTCCTCACCAACAAAACTCTGGGCCCA
CTGGGGGCCACCGAGGAAGACTTGGGCTCCACATTCCAATGTAAGCTCTGCAACCGGACT
TTTGCGAGCAAGCACGCAGTCAAACTGCACCTTAGTAAGACCCACGGCAAGTCTCCCGAG
GACCACCTGATCTATGTGACTGAGTTGGAGAAACAGTAA
Protein sequence predicted based on nucleotide sequence
MCNEETEIKEAQSYQNSPVSSATNQDAGYGSPFSESSDQLAHFKGSSSREEKEDPQCPDS VSYPQDSLAQIKAVYANLFSESCWSSLALDLKKSGSTTSTNDASQKESSAPTPTPPTCPV STTGPTTSTPSTSCSSSTSHSSTTSTSSSSGYDWHQAALAKTLQQTSSYGLLPEPSLFST VQLYRQNNKLYGSVFTGASKFRCKDCSAAYDTLVELTVHMNETGHYRDDNRDKDSEKTKR WSKPRKRSLMEMEGKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPLKEPVPAIT KLVPSTKKRALQDLAPPCSPEPAGMAAEVALSESAKDQKAANPYVTPNNRYGYQNGASYT WQFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFLKVTTSASKKGKQLVLDPVVEEKI QSIPLPPTTHTRLPASSIKKQPDSPAGSTTSEEKKEPEKEKPPVAGDAEKIKEESEDSLE KFEPSTLYPYLREEDLDDSPKGGLDILKSLENTVSTAISKAQNGAPSWGGYPSIHAAYQL PGTVKPLPAAVQSVQVQPSYAGGVKSLSSAEHNALLHSPGSLTPPPHKSNVSAMEELVEK VTGKVNIKKEERPPEKEKSSLAKAASPIAKENKDFPKTEEVSGKPQKKGPEAETGKAKKE GPLDVHTPNGTEPLKAKVTNGCNNLGIIMDHSPEPSFINPLSALQSIMNTHLGKVSKPVS PSLDPLAMLYKISNSMLDKPVYPATPVKQADAIDRYYYENSDQPIDLTKSKNKPLVSSVA DSVASPLRESALMDISDMVKNLTGRLTPKSSTPSTVSEKSDADGSSFEEALDELSPVHKR KGRQSNWNPQHLLILQAQFASSLRETTEGKYIMSDLGPQERVHISKFTGLSMTTISHWLA NVKYQLRRTGGTKFLKNLDTGHPVFFCNDCASQFRTASTYISHLETHLGFSLKDLSKLPL NQIQEQQNVSKVLTNKTLGPLGATEEDLGSTFQCKLCNRTFASKHAVKLHLSKTHGKSPE DHLIYVTELEKQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3662
Start on reference sequence
564
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
