Gene:
Gene Symbol:
AIM1
Gene Name:
absent in melanoma 1
Original Clone ID:
FLH269549.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082774
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAAGAGGTCCAGCGGCCGTAGGTCGGGGAGGCGGAGGGGGTCGCAGAAATCCACC
GACTCCCCCGGCGCGGACGCCGAGCTCCCTGAGAGCGCTGCCAGGGACGACGCGGTGTTC
GACGACGAGGTGGCGCCAAACGCGGCCAGCGATAACGCCTCGGCGGAAAAGAAAGTGAAA
TCTCCGCGGGCAGCCCTCGACGGGGGCGTTGCCTCCGCTGCGAGCCCAGAGTCCAAGCCC
AGCCCCGGTACCAAAGGGCAGCTCCGAGGGGAGTCGGACCGGAGCAAACAGCCACCCCCG
GCTTCGTCCCCCACGAAGAGGAAGGGCAGGAGCCGTGCCCTCGAGGCCGTGCCCGCCCCG
CCCGCCAGCGGCCCCCGGGCTCCCGCCAAGGAGTCCCCACCCAAGAGGGTGCCCGATCCC
AGCCCAGTCACCAAGGGCACTGCGGCCGAGAGCGGGGAGGAGGCGGCGCGGGCCATCCCC
CGCGAGCTCCCGGTCAAGAGCAGCTCGCTGCTGCCGGAGATCAAGCCCGAGCACAAGAGG
GGCCCGCTCCCCAACCACTTCAACGGCCGGGCAGAGGGAGGTCGAAGCAGAGAGCTGGGC
AGAGCGGCCGGAGCGCCTGGAGCTTCTGACGCCGACGGCTTGAAGCCCAGGAACCATTTC
GGCGTGGGCAGGTCGACAGTGACCACTAAAGTGACCCTCCCTGCCAAGCCCAAACATGTG
GAACTAAATCTTAAAACCCCTAAGAATCTTGACAGTTTGGGAAATGAGCACAATCCATTT
AGCCAGCCAGTTCACAAAGGCAACACTGCCACCAAAATCTCCTTATTTGAAAACAAACGG
ACAAACAGTAGCCCAAGACACACTGACATTCGAGGCCAAAGGAATACTCCTGCCTCTAGT
AAAACGTTTGTTGGGAGGGCAAAGCTGAATTTAGCCAAAAAAGCCAAAGAAATGGAGCAA
CCTGAAAAGAAAGTAATGCCAAACAGTCCCCAGAATGGTGTGCTGGTTAAGGAAACTGCT
ATAGAAACCAAAGTTACCGTCTCGGAAGAAGAGATTCTGCCAGCAACCAGAGGAATGAAT
GGAGACTCTTCTGAGAATCAAGCTCTTGGTCCTCAGCCTAACCAAGATGATAAAGCAGAT
GTACAAACAGATGCTGGCTGCCTTTCAGAACCAGTGGCTTCTGCTCTGATTCCTGTCAAG
GATCATAAGCTCTTAGAGAAGGAGGACTCAGAGGCTGCAGACAGCAAAAGCCTTGTACTT
GAAAATGTAACCGATACAGCACAAGACATCCCCACCACTGTGGATACCAAAGATTTACCT
CCAACGGCCATGCCAAAGCCACAGCATACATTTTCTGACTCACAGTCCCCTGCTGAGTCA
TCTCCTGGGCCTTCTCTTTCACTGTCTGCACCCGCTCCTGGGGATGTTCCCAAAGACACA
TGTGTTCAATCACCCATAAGCAGTTTCCCATGCACTGATCTAAAAGTGTCAGAAAACCAT
AAAGGATGTGTTTTGCCTGTGTCTCGTCAGAACAATGAGAAAATGCCACTTTTAGAACTT
GGAGGAGAAACAACCCCTCCTTTGTCCACAGAGCGTAGTCCAGAAGCTGTGGGAAGTGAG
TGTCCATCCAGAGTCCTCGTCCAGGTCAGGTCCTTCGTGCTCCCCGTGGAGAGCACCCAG
GATGTGAGCTCCCAGGTCATCCCAGAGAGCTCTGAAGTTAGAGAAGTGCAGTTGCCAACT
TGTCACAGTAATGAACCTGAAGTGGTTTCCGTTGCAAGTTGTGCTCCCCCACAAGAGGAA
GTACTGGGCAATGAACACTCTCATTGCACAGCAGAGCTCGCGGCAAAATCTGGCCCACAA
GTCATACCGCCAGCATCAGAGAAAACTCTGCCTATTCAGGCTCAAAGTCAGGGCAGCAGA
ACACCCCTGATGGCTGAATCCAGTCCCACCAACTCTCCCAGCAGCGGAAATCACTTAGCC
ACTCCTCAAAGGCCAGATCAGACTGTTACAAATGGCCAGGATAGCCCTGCCAGCCTTTTG
AACATTTCTGCTGGTAGTGATGATAGTGTATTTGATTCTTCTTCTGATATGGAAAAATTC
ACTGAAATTATAAAACAGATGGATAGCGCAGTTTGTATGCCCATGAAAAGAAAGAAGGCC
AGGATGCCAAACTCTCCTGCTCCTCACTTTGCCATGCCTCCTATTCACGAAGACCATTTA
GAAAAGGTGTTTGATCCCAAAGTGTTTACCTTTGGTTTGGGGAAGAAGAAGGAAAGTCAG
CCAGAAATGTCACCGGCTTTACATTTGATGCAGAACCTTGACACAAAATCCAAACTGAGA
CCCAAACGTGCATCTGCTGAACAGAGCGTCCTCTTCAAGTCCCTGCACACCAACACTAAT
GGGAACAGTGAGCCTCTGGTGATGCCGGAAATCAATGACAAAGAGAACAGGGACGTCACA
AATGGTGGCATTAAGAGATCGAGACTAGAAAAAAGTGCACTTTTCTCAAGCTTGTTATCT
TCTTTACCACAAGACAAAATCTTTTCTCCTTCTGTGACATCAGTCAACACTATGACCACG
GCTTTCAGTACTTCTCAGAACGGTTCCCTATCTCAGTCTTCAGTGTCACAGCCCACGACT
GAGGGTGCCCCGCCCTGTGGTTTGAACAAAGAACAGTCAAATCTTCTGCCCGACAACTCC
TTAAAGGTCTTCAATTTCAACTCGTCAAGTACATCACACTCCAGTTTGAAAAGTCCAAGC
CACATGGAAAAATACCCGCAAAAAGAGAAAACCAAAGAAGATCTGGATTCACGAAGCAAC
CTACACTTGCCAGAAACTAAATTTTCTGAATTGTCAAAACTGAAGAATGATGATATGGAA
AAGGCTAATCATATTGAAAGTGTTATTAAATCAAACTTGCCAAACTGTGCAAACAGTGAC
ACCGACTTCATGGGTCTTTTCAAATCAAGCCGGTATGACCCAAGCATTTCTTTTTCTGGA
ATGTCATTATCAGACACAATGACACTTAGAGGAAGTGTCCAAAATAAACTCAATCCCCGA
CCTGGAAAGGTAGTGATATATAGTGAACCCGACGTCTCTGAGAAGTGCATTGAAGTTTTC
AGTGACATTCAGGATTGCAGTTCTTGGAGCCTCTCTCCAGTGATACTCATAAAAGTTGTT
AGAGGATGTTGGATTTTGTATGAGCAACCAAATTTTGAAGGGCACTCCATCCCCTTAGAA
GAAGGAGAATTGGAACTCTCTGGTCTCTGGGGTATAGAAGACATTTTGGAAAGGCACGAA
GAAGCAGAGTCTGATAAGCCAGTGGTGATTGGTTCCATCAGACATGTGGTTCAGGATTAC
AGAGTTAGTCACATTGACTTATTTACTGAACCAGAAGGGTTAGGAATCCTAAGTTCCTAC
TTTGATGATACTGAAGAAATGCAGGGATTTGGTGTAATGCAGAAGACTTGTTCCATGAAA
GTACATTGGGGCACGTGGCTGATTTATGAAGAACCTGGATTTCAGGGTGTTCCTTTCATC
CTGGAACCTGGTGAATACCCTGACTTGTCCTTCTGGGATACAGAAGCAGCGTACATTGGA
TCCATGCGGCCTCTGAAAATGGGTGGCCGTAAAGTTGAATTCCCTACAGATCCAAAGGTA
GTTGTTTATGAAAAGCCTTTCTTTGAAGGAAAATGTGTGGAACTAGAAACAGGAATGTGT
AGTTTTGTCATGGAGGGAGGTGAAACAGAAGAGGCGACTGGAGACGATCATTTGCCGTTT
ACGTCAGTGGGGTCTATGAAAGTTCTAAGAGGCATTTGGGTTGCATATGAGAAACCTGGA
TTTACCGGTCATCAGTATTTGCTAGAAGAAGGAGAATACAGGGACTGGAAAGCCTGGGGA
GGTTACAATGGAGAGCTTCAGTCTTTACGACCTATATTAGGTGATTTTTCAAATGCTCAC
ATGATAATGTACAGTGAAAAAAACTTTGGATCCAAAGGTTCCAGTATTGATGTATTGGGA
ATTGTTGCTAATTTAAAGGAGACTGGATATGGAGTGAAGACACAGTCTATTAATGTACTG
AGTGGAGTATGGGTAGCCTATGAAAATCCTGACTTCACAGGAGAACAGTATATACTGGAT
AAAGGATTTTATACCAGTTTTGAGGACTGGGGAGGCAAAAATTGTAAGATCTCTTCTGTT
CAACCTATATGTTTGGATTCTTTCACTGGCCCAAGGAGACGAAATCAGATTCACTTGTTT
TCAGAACCACAGTTTCAAGGTCACAGTCAAAGTTTTGAAGAAACAACAAGTCAAATTGAT
GATTCATTTTCTACCAAGTCTTGCAGAGTTTCAGGAGGCAGCTGGGTTGTATATGATGGA
GAAAATTTCACTGGTAATCAATACGTGTTGGAAGAAGGCCATTATCCTTGTCTGTCTGCA
ATGGGATGCCCGCCTGGAGCAACTTTCAAGTCTCTTCGTTTTATAGATGTTGAATTTTCT
GAACCAACAATTATTCTCTTTGAAAGAGAAGACTTCAAAGGAAAAAAGATTGAACTTAAT
GCAGAAACTGTCAATCTCCGATCCCTGGGATTCAACACACAAATACGCTCTGTTCAGGTT
ATTGGTGGCATATGGGTTACTTATGAATATGGCAGTTACAGAGGGCGACAGTTCCTATTG
TCACCTGCAGAAGTACCTAATTGGTATGAATTCAGTGGCTGTCGCCAAATAGGTTCTCTA
CGACCTTTTGTTCAGAAGCGAATTTATTTCAGACTTCGAAACAAAGCAACAGGGTTATTC
ATGTCAACCAATGGAAACTTAGAGGATCTGAAGCTTCTGAGGATACAGGTCATGGAGGAT
GTCGGGGCCGATGATCAGATTTGGATCTATCAAGAAGGATGTATCAAATGCAGGATAGCA
GAAGACTGCTGCCTGACGATTGTGGGCAGCCTGGTAACATCTGGCTCCAAGCTAGGCCTG
GCCCTGGACCAGAATGCTGACAGCCAGTTCTGGAGCTTGAAGTCCGATGGCAGGATTTAC
AGCAAGTTGAAGCCAAATTTAGTTTTAGACATTAAAGGGGGCACACAGTATGATCAAAAT
CACATTATCCTCAACACTGTCAGCAAAGAGAAGTTTACACAAGTGTGGGAAGCCATGGTC
CTATATACCTAA
Protein sequence predicted based on nucleotide sequence
MEKRSSGRRSGRRRGSQKSTDSPGADAELPESAARDDAVFDDEVAPNAASDNASAEKKVK SPRAALDGGVASAASPESKPSPGTKGQLRGESDRSKQPPPASSPTKRKGRSRALEAVPAP PASGPRAPAKESPPKRVPDPSPVTKGTAAESGEEAARAIPRELPVKSSSLLPEIKPEHKR GPLPNHFNGRAEGGRSRELGRAAGAPGASDADGLKPRNHFGVGRSTVTTKVTLPAKPKHV ELNLKTPKNLDSLGNEHNPFSQPVHKGNTATKISLFENKRTNSSPRHTDIRGQRNTPASS KTFVGRAKLNLAKKAKEMEQPEKKVMPNSPQNGVLVKETAIETKVTVSEEEILPATRGMN GDSSENQALGPQPNQDDKADVQTDAGCLSEPVASALIPVKDHKLLEKEDSEAADSKSLVL ENVTDTAQDIPTTVDTKDLPPTAMPKPQHTFSDSQSPAESSPGPSLSLSAPAPGDVPKDT CVQSPISSFPCTDLKVSENHKGCVLPVSRQNNEKMPLLELGGETTPPLSTERSPEAVGSE CPSRVLVQVRSFVLPVESTQDVSSQVIPESSEVREVQLPTCHSNEPEVVSVASCAPPQEE VLGNEHSHCTAELAAKSGPQVIPPASEKTLPIQAQSQGSRTPLMAESSPTNSPSSGNHLA TPQRPDQTVTNGQDSPASLLNISAGSDDSVFDSSSDMEKFTEIIKQMDSAVCMPMKRKKA RMPNSPAPHFAMPPIHEDHLEKVFDPKVFTFGLGKKKESQPEMSPALHLMQNLDTKSKLR PKRASAEQSVLFKSLHTNTNGNSEPLVMPEINDKENRDVTNGGIKRSRLEKSALFSSLLS SLPQDKIFSPSVTSVNTMTTAFSTSQNGSLSQSSVSQPTTEGAPPCGLNKEQSNLLPDNS LKVFNFNSSSTSHSSLKSPSHMEKYPQKEKTKEDLDSRSNLHLPETKFSELSKLKNDDME KANHIESVIKSNLPNCANSDTDFMGLFKSSRYDPSISFSGMSLSDTMTLRGSVQNKLNPR PGKVVIYSEPDVSEKCIEVFSDIQDCSSWSLSPVILIKVVRGCWILYEQPNFEGHSIPLE EGELELSGLWGIEDILERHEEAESDKPVVIGSIRHVVQDYRVSHIDLFTEPEGLGILSSY FDDTEEMQGFGVMQKTCSMKVHWGTWLIYEEPGFQGVPFILEPGEYPDLSFWDTEAAYIG SMRPLKMGGRKVEFPTDPKVVVYEKPFFEGKCVELETGMCSFVMEGGETEEATGDDHLPF TSVGSMKVLRGIWVAYEKPGFTGHQYLLEEGEYRDWKAWGGYNGELQSLRPILGDFSNAH MIMYSEKNFGSKGSSIDVLGIVANLKETGYGVKTQSINVLSGVWVAYENPDFTGEQYILD KGFYTSFEDWGGKNCKISSVQPICLDSFTGPRRRNQIHLFSEPQFQGHSQSFEETTSQID DSFSTKSCRVSGGSWVVYDGENFTGNQYVLEEGHYPCLSAMGCPPGATFKSLRFIDVEFS EPTIILFEREDFKGKKIELNAETVNLRSLGFNTQIRSVQVIGGIWVTYEYGSYRGRQFLL SPAEVPNWYEFSGCRQIGSLRPFVQKRIYFRLRNKATGLFMSTNGNLEDLKLLRIQVMED VGADDQIWIYQEGCIKCRIAEDCCLTIVGSLVTSGSKLGLALDQNADSQFWSLKSDGRIY SKLKPNLVLDIKGGTQYDQNHIILNTVSKEKFTQVWEAMVLYT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
488
End on reference sequence
5659
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
