Gene:
Gene Symbol:
DLG2
Gene Name:
discs, large homolog 2 (Drosophila)
Original Clone ID:
FLH269615.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082836
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTTCTTTGCATGTTACTGTGCACTCCGGACTAACGTGAAGAAGTATCGATATCAAGAT
GAGGACGCTCCACATGATCATTCCTTACCTCGACTAACCCACGAAGTAAGAGGCCCAGAA
CTCGTGCATGTATCAGAAAAGAACCTCTCTCAAATAGAAAATGTCCATGGATATGTCTTG
CAGTCTCATATTTCTCCTCTGAAGGCCAGTCCTGCTCCTATAATTGTCAACACAGATACT
TTGGACACAATTCCTTATGTCAATGGGACAGAAATTGAATATGAATTTGAAGAAATTACA
CTGGAGAGGGGGAATTCTGGCCTGGGATTCAGTATTGCTGGGGGGACAGATAATCCCCAC
ATTGGAGATGACCCTGGCATATTTATTACGAAGATTATACCAGGAGGTGCTGCAGCAGAG
GATGGCAGACTCAGGGTCAATGATTGTATCTTGCGGGTGAATGAGGTTGATGTGTCAGAG
GTTTCCCACAGTAAAGCGGTGGAAGCCCTGAAGGAAGCAGGGTCTATCGTTCGGCTGTAT
GTGCGTAGAAGACGACCTATTTTGGAGACCGTTGTGGAAATCAAACTGTTCAAAGGCCCT
AAAGGTTTAGGCTTCAGTATTGCAGGAGGTGTGGGGAACCAACACATTCCTGGAGACAAC
AGCATTTATGTAACTAAAATTATAGATGGAGGAGCTGCACAAAAAGATGGAAGGTTGCAA
GTAGGAGATAGACTACTAATGGTAAACAACTACAGTTTAGAAGAAGTAACACACGAAGAG
GCAGTAGCAATATTAAAGAACACATCAGAGGTAGTTTATTTAAAAGTTGGCAAACCCACT
ACCATTTATATGACTGATCCTTATGGTCCACCTGATATTACTCACTCTTATTCTCCACCA
ATGGAAAACCATCTACTCTCTGGCAACAATGGCACTTTAGAATATAAAACCTCCCTGCCA
CCCATCTCTCCAGGAAGGTACTCACCAATTCCAAAGCACATGCTTGTTGACGACGACTAC
ACCAGGCCTCCGGAACCTGTTTACAGCACTGTGAACAAACTATGTGATAAGCCTGCTTCT
CCCAGGCACTATTCCCCTGTTGAGTGTGACAAAAGCTTCCTCCTCTCAGCTCCCTATTCC
CACTACCACCTAGGCCTGCTACCTGACTCTGAGATGACCAGTCATTCCCAACATAGCACC
GCAACTCGTCAGCCTTCAATGACTCTCCAACGGGCCGTCTCCCTGGAAGGAGAGCCTCGC
AAGGTAGTCCTGCACAAAGGCTCCACTGGCCTGGGCTTCAACATTGTCGGTGGGGAAGAT
GGAGAAGGTATTTTTGTGTCCTTCATTCTGGCTGGTGGACCAGCAGACCTAAGTGGGGAG
CTCCAGAGAGGAGACCAGATCCTATCGGTGAATGGCATTGACCTCCGTGGTGCATCCCAC
GAGCAGGCAGCTGCTGCACTAAAGGGGGCTGGACAGACAGTGACGATTATAGCACAATAT
CAACCTGAAGATTACGCTCGATTTGAGGCCAAAATCCATGACCTACGAGAGCAGATGATG
AACCACAGCATGAGCTCCGGGTCCGGATCCCTGCGAACCAATCAGAAACGCTCCCTCTAC
GTCAGAGCCATGTTCGACTACGACAAGAGCAAGGACAGTGGGCTGCCAAGTCAAGGACTT
AGTTTTAAATATGGAGATATTCTCCACGTTATCAATGCCTCTGATGATGAGTGGTGGCAA
GCCAGGAGAGTCATGCTGGAGGGAGACAGTGAGGAGATGGGGGTCATCCCCAGCAAAAGG
AGGGTGGAAAGAAAGGAACGTGCCCGATTGAAGACAGTGAAGTTTAATGCCAAACCTGGA
GTGATTGATTCGAAAGGGTCATTCAATGACAAGCGTAAAAAGAGCTTCATCTTTTCACGA
AAATTCCCATTCTACAAGAACAAGGAGCAGAGTGAGCAGGAAACCAGTGATCCTGAACGA
GGACAAGAAGACCTCATTCTTTCCTATGAGCCTGTTACAAGGCAGGAAATAAACTACACC
CGGCCGGTGATTATCCTGGGGCCCATGAAGGATCGGATCAATGACGACTTGATATCTGAA
TTCCCTGATAAATTTGGCTCCTGTGTGCCTCATACTACGAGGCCAAAGCGAGACTACGAG
GTGGATGGCAGAGACTATCACTTTGTCATTTCCAGAGAACAAATGGAGAAAGATATCCAA
GAGCACAAGTTTATAGAAGCCGGCCAGTACAATGACAATTTATATGGAACCAGTGTGCAG
TCTGTGAGATTTGTAGCAGAAAGAGGCAAACACTGTATACTTGATGTATCAGGAAATGCT
ATCAAGCGGTTACAAGTTGCCCAGCTCTATCCCATTGCCATCTTCATAAAACCCAGGTCT
CTGGAACCTCTTATGGAGATGAATAAGCGTCTAACAGAGGAACAAGCCAAGAAAACCTAT
GATCGAGCAATTAAGCTAGAACAAGAATTTGGAGAATATTTTACAGCTATTGTCCAAGGA
GATACTTTAGAAGATATATATAACCAATGCAAGCTTGTTATTGAAGAGCAATCTGGGCCT
TTCATCTGGATTCCCTCAAAGGAAAAGTTA
Protein sequence predicted based on nucleotide sequence
MFFACYCALRTNVKKYRYQDEDAPHDHSLPRLTHEVRGPELVHVSEKNLSQIENVHGYVL QSHISPLKASPAPIIVNTDTLDTIPYVNGTEIEYEFEEITLERGNSGLGFSIAGGTDNPH IGDDPGIFITKIIPGGAAAEDGRLRVNDCILRVNEVDVSEVSHSKAVEALKEAGSIVRLY VRRRRPILETVVEIKLFKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIDGGAAQKDGRLQ VGDRLLMVNNYSLEEVTHEEAVAILKNTSEVVYLKVGKPTTIYMTDPYGPPDITHSYSPP MENHLLSGNNGTLEYKTSLPPISPGRYSPIPKHMLVDDDYTRPPEPVYSTVNKLCDKPAS PRHYSPVECDKSFLLSAPYSHYHLGLLPDSEMTSHSQHSTATRQPSMTLQRAVSLEGEPR KVVLHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELQRGDQILSVNGIDLRGASH EQAAAALKGAGQTVTIIAQYQPEDYARFEAKIHDLREQMMNHSMSSGSGSLRTNQKRSLY VRAMFDYDKSKDSGLPSQGLSFKYGDILHVINASDDEWWQARRVMLEGDSEEMGVIPSKR RVERKERARLKTVKFNAKPGVIDSKGSFNDKRKKSFIFSRKFPFYKNKEQSEQETSDPER GQEDLILSYEPVTRQEINYTRPVIILGPMKDRINDDLISEFPDKFGSCVPHTTRPKRDYE VDGRDYHFVISREQMEKDIQEHKFIEAGQYNDNLYGTSVQSVRFVAERGKHCILDVSGNA IKRLQVAQLYPIAIFIKPRSLEPLMEMNKRLTEEQAKKTYDRAIKLEQEFGEYFTAIVQG DTLEDIYNQCKLVIEEQSGPFIWIPSKEKL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2916
Start on reference sequence
304
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
