DNASU Plasmid | POU3F1 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: POU3F1

Gene Name: POU class 3 homeobox 1

Original Clone ID:

FLH269670.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00082889

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1353nts

Open reading frame: 1 to 1353

Reference Sequence Alignment

ATGGCCACCACCGCGCAGTACCTGCCGCGGGGCCCCGGTGGCGGAGCCGGGGGCACCGGG
CCGCTTATGCACCCGGACGCCGCGGCGGCGGCGGCGGCGGCGGCGGCCGCGGAGCGATTG
CATGCAGGGGCCGCGTACCGCGAAGTGCAGAAGCTGATGCACCACGAGTGGCTGGGCGCG
GGCGCGGGCCACCCCGTGGGCCTAGCGCACCCCCAGTGGCTACCCACGGGAGGAGGCGGC
GGCGGCGATTGGGCCGGCGGCCCGCACCTAGAACACGGCAAGGCAGGCGGCGGCGGCACC
GGCCGAGCCGACGACGGCGGCGGCGGCGGAGGTTTCCACGCGCGCCTGGTGCACCAGGGG
GCGGCCCACGCGGGCGCGGCATGGGCGCAGGGCAGCACAGCGCACCACTTGGGCCCGGCC
ATGTCGCCCTCGCCCGGGGCCAGCGGGGGCCACCAGCCCCAGCCGCTCGGGCTGTACGCG
CAGGCGGCCTACCCGGGGGGCGGCGGCGGCGGCCTGGCCGGGATGCTGGCGGCGGGCGGT
GGCGGCGCGGGGCCGGGCCTGCACCACGCGCTGCACGAGGATGGCCACGAGGCGCAGCTG
GAGCCGTCGCCGCCGCCGCATCTGGGCGCCCACGGACACGCACACGGACATGCACACGCG
GGCGGCCTGCACGCGGCGGCGGCGCACCTGCACCCGGGCGCGGGCGGCGGCGGCTCATCG
GTGGGCGAGCACTCGGACGAGGATGCGCCCAGCTCGGACGACCTGGAGCAGTTCGCCAAG
CAGTTCAAGCAGCGGCGCATCAAGCTGGGCTTTACGCAGGCCGACGTGGGGCTGGCGCTG
GGCACGCTCTACGGTAACGTGTTCTCGCAGACCACCATCTGCCGCTTCGAGGCCCTGCAG
CTGAGCTTCAAGAACATGTGCAAGCTCAAGCCGCTGCTCAACAAGTGGCTGGAGGAGACC
GACTCGTCCAGCGGCAGCCCCACCAACCTGGACAAGATCGCGGCGCAGGGCCGCAAGCGC
AAGAAGCGCACGTCCATCGAGGTGGGGGTCAAAGGCGCGCTCGAGAGCCACTTTCTCAAG
TGCCCCAAGCCCTCGGCGCACGAGATCACCGGCTTGGCAGACAGCCTGCAGCTGGAGAAG
GAGGTGGTGCGCGTCTGGTTCTGCAACCGGCGGCAGAAGGAGAAGCGCATGACCCCTGCG
GCCGGCGCGGGCCACCCGCCCATGGACGATGTATACGCGCCTGGGGAGCTAGGGCCTGGC
GGGGGCGGCGCATCGCCACCCTCCGCGCCCCCACCGCCCCCGCCGGCGGCGCTGCACCAC
CACCACCACCACACACTGCCCGGCTCAGTGCAG

Protein sequence predicted based on nucleotide sequence

MATTAQYLPRGPGGGAGGTGPLMHPDAAAAAAAAAAAERLHAGAAYREVQKLMHHEWLGA
GAGHPVGLAHPQWLPTGGGGGGDWAGGPHLEHGKAGGGGTGRADDGGGGGGFHARLVHQG
AAHAGAAWAQGSTAHHLGPAMSPSPGASGGHQPQPLGLYAQAAYPGGGGGGLAGMLAAGG
GGAGPGLHHALHEDGHEAQLEPSPPPHLGAHGHAHGHAHAGGLHAAAAHLHPGAGGGGSS
VGEHSDEDAPSSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQ
LSFKNMCKLKPLLNKWLEETDSSSGSPTNLDKIAAQGRKRKKRTSIEVGVKGALESHFLK
CPKPSAHEITGLADSLQLEKEVVRVWFCNRRQKEKRMTPAAGAGHPPMDDVYAPGELGPG
GGGASPPSAPPPPPPAALHHHHHHTLPGSVQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1391

Start on reference sequence 36

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

POU3F1

Symbol Nomenclature :

POU3F1

SYNONYM :

OCT6; OTF6; SCIP

Designation :

OTF-6|POU domain transcription factor SCIP|POU domain, class 3, transcription factor 1|octamer-binding protein 6|octamer-binding transcription factor 6

Full Nomenclature :

POU class 3 homeobox 1

GENEID :

5453

GI :

162318521

GenBank Accession :

BC156294

HGNC :

9214

MIM :

602479

Vega :

OTTHUMG00000000485

Target GenBank:

NM_002699

Reference Sequence Alignment

  

HsCD00082889      ------ATGGCCACCACCGCGCAGTACCTGCCGCGGGGCCCCGGTGGCGGAGCCGGGGGC
NM_005604.3       ATGGCGACCGCAGCGTCTAACCACTACAGCCTGCTCACCTCCAGCGCCTCCATCGTGCAC
NM_002699.3       ------ATGGCCACCACCGCGCAGTACCTGCCGCGGGGCCCCGGTGGCGGAGCCGGGGGC
                        *  **..* :* .. ** ***.  * **  . * **.* * *  .. ** * .*

HsCD00082889      ACCGGGCCGCTTATGCACCCGGACGCCGCGGCGGCGG---------------------CG
NM_005604.3       GCCGAGCCGCCCGGCGGCATGCAGCAGGGCGCGGGGGGCTACCGCGAAGCGCAGAGCCTG
NM_002699.3       ACCGGGCCGCTTATGCACCCGGACGCCGCGGCGGCGG---------------------CG
                  .***.*****  .   .*. * *  . *  **** **                      *

HsCD00082889      GCGGCGGCGGCGGCCGCGGAGCGATTGCATGCAGGGGCCGCGTACCGCGAAGTGCAGAAG
NM_005604.3       GTGCAGGGCGACTACGGCGCTCTGCAGAGCAACGGACACCCGCTCAGCCACGCTCACCAG
NM_002699.3       GCGGCGGCGGCGGCCGCGGAGCGATTGCATGCAGGGGCCGCGTACCGCGAAGTGCAGAAG
                  * * .**  *.  .**  *. * . :*.. ...**. .* ** :*.** *.*  ** .**

HsCD00082889      CTGATGCACCACGAGTGGCTGGGCGCGGGCGCGGGCCACCCCGTGGGCCTAGCGCACCCC
NM_005604.3       TGGATCACCGCGCTGTCCCACGGCGGCGGCGGCGGGGGCGGTGGCGGCGGCGGGGGGGGC
NM_002699.3       CTGATGCACCACGAGTGGCTGGGCGCGGGCGCGGGCCACCCCGTGGGCCTAGCGCACCCC
                    *** ..* .  :**  *: ****  ****  **  .*   *  ***  .* * .   *

HsCD00082889      CAGTGGCTACCCACGGGAGGAGGCGGCGGCGGCGATTGGGCCGGCGGCCCGCACCTAGAA
NM_005604.3       GGGGGCGGCGGCGGGGGCGGCGGCGACGGCTCCCCGTGGTCCACCAGCCCCCTGGGCCAG
NM_002699.3       CAGTGGCTACCCACGGGAGGAGGCGGCGGCGGCGATTGGGCCGGCGGCCCGCACCTAGAA
                   .* *   .  *. ***.**.****.****  * . *** **. *.**** *:   . *.

HsCD00082889      CACGGCAAGGCAGGCGGCGGCGGCACCGGCCGAGCCGACGACGGCGGCGGCGGCGGAGGT
NM_005604.3       CCGGACATCAAGCCCTCGGTGGTGGTGCAGCAGGGCGGCCGCGGAGACGAGCTGCACGGG
NM_002699.3       CACGGCAAGGCAGGCGGCGGCGGCACCGGCCGAGCCGACGACGGCGGCGGCGGCGGAGGT
                  *. *.**: ...  *   *  *  .   . *..* **.* .***.*.**.     ..** 

HsCD00082889      TTCCACGCGCGCCTGGTGCACCAGGGGGCGGCCCACGCGGGCGCGGCATGGGCGCAGGGC
NM_005604.3       CCAGGCGCCCTGCAGCAGCAGCATCAGCAGCAGCAACAGCAACAGCAGCAGCAACAGCAG
NM_002699.3       TTCCACGCGCGCCTGGTGCACCAGGGGGCGGCCCACGCGGGCGCGGCATGGGCGCAGGGC
                    . .*** *  *:* :*** **  .* .* . **. .* .. .* .. .* ..*** . 

HsCD00082889      AGCACAGCGCACCACTTGGGCCCGGCCATGTCGCCCTCGCCCGGGGCCAGCGGGGGCCAC
NM_005604.3       CAACAGCAGCAGCAGCAGCAGCAACAGCGGCCGCCGCATCTGGTGCACCACGCCGCTAAC
NM_002699.3       AGCACAGCGCACCACTTGGGCCCGGCCATGTCGCCCTCGCCCGGGGCCAGCGGGGGCCAC
                  ...... .*** **  :* . *.. . . * ****  . *  * * .*..**  *  .**

HsCD00082889      CAGCCCCAGCCGCTCGGGCTGTACGCGCAGGCG---------------------GCCTAC
NM_005604.3       CACCACCCGGGACCCGGGGCATGGCGGAGCGCGGCGGCTGCAGCGCACCTCCCACCCTCC
NM_002699.3       CAGCCCCAGCCGCTCGGGCTGTACGCGCAGGCG---------------------GCCTAC
                  ** *.**.*  .* ****  .*.   *.. ***                      ***.*

HsCD00082889      CCGGGGGGCGGCGGCGGCGGCCTGGCCGGGATGCTGGCGGCGGGCGGTGGCGGCGCGGGG
NM_005604.3       ATGGGAGCGTCCAACGGCGGCTTGCTCTACTCGCAGCCCAGCTTCACGGTGAACGGCATG
NM_002699.3       CCGGGGGGCGGCGGCGGCGGCCTGGCCGGGATGCTGGCGGCGGGCGGTGGCGGCGCGGGG
                  . ***.*    *..******* **  * . : **:* * .    *.  *  ..**  . *

HsCD00082889      CCGGGCCTGCACCACGCGCTGCACGAGGATGGCCACGAGGCGCAGCTGGAGCCGTCGCCG
NM_005604.3       CTGGGCGCCGGCGGGCAGCCGGCCGGTCTGCACCACCACGGCCTGCGGGACGCGCACGAC
NM_002699.3       CCGGGCCTGCACCACGCGCTGCACGAGGATGGCCACGAGGCGCAGCTGGAGCCGTCGCCG
                  * ****    .* .  .** * .**.  :  .**** * *  *:** ***  ** .  . 

HsCD00082889      CCGCCGCATCTGGGCGCCCACGGACACGCACACGGACATGCACACGCGGGCGGCCTGCAC
NM_005604.3       GAGCCACACCATG---CCGACCACCACCCGCACCCGCACTCGCACCCACACCAGCAGCCG
NM_002699.3       CCGCCGCATCTGGGCGCCCACGGACACGCACACGGACATGCACACGCGGGCGGCCTGCAC
                   .***.** *: *   ** ** ..*** *.***  .**  *.*** *. .* . *:**. 

HsCD00082889      GCGGCGGCGGCGCACCTGCACCCGGGCGCGGGCGGCGGCGGCTCATCGGTGGGCGAGCAC
NM_005604.3       CCGCCCCCGCCGCCCCCGCAGGGTCCGCCTGGCCACCCAGGCGCGCACCACGACCCGCAC
NM_002699.3       GCGGCGGCGGCGCACCTGCACCCGGGCGCGGGCGGCGGCGGCTCATCGGTGGGCGAGCAC
                   ** *  ** ***.** ***        * *** .*  .*** *. .  : *.* .****

HsCD00082889      TCGGACGAGGATGCGCCCAGCTCGGACGACCTGGAGCAGTTCGCCAAGCAGTTCAAGCAG
NM_005604.3       TCGGACGAGGACACGCCGACCTCGGACGACCTGGAGCAGTTCGCCAAGCAGTTCAAGCAG
NM_002699.3       TCGGACGAGGATGCGCCCAGCTCGGACGACCTGGAGCAGTTCGCCAAGCAGTTCAAGCAG
                  *********** .**** * ****************************************

HsCD00082889      CGGCGCATCAAGCTGGGCTTTACGCAGGCCGACGTGGGGCTGGCGCTGGGCACGCTCTAC
NM_005604.3       CGGCGGATCAAACTGGGATTTACCCAAGCGGACGTGGGGCTGGCTCTGGGCACCCTGTAT
NM_002699.3       CGGCGCATCAAGCTGGGCTTTACGCAGGCCGACGTGGGGCTGGCGCTGGGCACGCTCTAC
                  ***** *****.*****.***** **.** ************** ******** ** ** 

HsCD00082889      GGTAACGTGTTCTCGCAGACCACCATCTGCCGCTTCGAGGCCCTGCAGCTGAGCTTCAAG
NM_005604.3       GGCAACGTGTTCTCGCAGACCACCATCTGCAGGTTTGAGGCCCTGCAGCTGAGCTTCAAG
NM_002699.3       GGTAACGTGTTCTCGCAGACCACCATCTGCCGCTTCGAGGCCCTGCAGCTGAGCTTCAAG
                  ** ***************************.* ** ************************

HsCD00082889      AACATGTGCAAGCTCAAGCCGCTGCTCAACAAGTGGCTGGAGGAGACCGACTCGTCCAGC
NM_005604.3       AACATGTGCAAGCTGAAGCCTTTGTTGAACAAGTGGTTGGAGGAGGCGGACTCGTCCTCG
NM_002699.3       AACATGTGCAAGCTCAAGCCGCTGCTCAACAAGTGGCTGGAGGAGACCGACTCGTCCAGC
                  ************** *****  ** * ********* ********.* *********:  

HsCD00082889      GGCAGCCCCACCAACCTGGACAAGATCGCGGCGCAGGGCCGCAAGCGCAAGAAGCGCACG
NM_005604.3       GGCAGCCCCACGAGCATAGACAAGATCGCAGCGCAAGGGCGCAAGCGGAAAAAGCGGACC
NM_002699.3       GGCAGCCCCACCAACCTGGACAAGATCGCGGCGCAGGGCCGCAAGCGCAAGAAGCGCACG
                  *********** *.*.*.***********.*****.** ******** **.***** ** 

HsCD00082889      TCCATCGAGGTGGGGGTCAAAGGCGCGCTCGAGAGCCACTTTCTCAAGTGCCCCAAGCCC
NM_005604.3       TCCATCGAGGTGAGCGTCAAGGGGGCTCTGGAGAGCCATTTCCTCAAATGCCCCAAGCCC
NM_002699.3       TCCATCGAGGTGGGGGTCAAAGGCGCGCTCGAGAGCCACTTTCTCAAGTGCCCCAAGCCC
                  ************.* *****.** ** ** ******** ** *****.************

HsCD00082889      TCGGCGCACGAGATCACCGGCTTGGCAGACAGCCTGCAGCTGGAGAAGGAGGTGGTGCGC
NM_005604.3       TCGGCCCAGGAGATCACCTCCCTCGCGGACAGCTTACAGCTGGAGAAGGAGGTGGTGAGA
NM_002699.3       TCGGCGCACGAGATCACCGGCTTGGCAGACAGCCTGCAGCTGGAGAAGGAGGTGGTGCGC
                  ***** ** *********  * * **.****** *.*********************.*.

HsCD00082889      GTCTGGTTCTGCAACCGGCGGCAGAAGGAGAAGCGCATGACCCCTGCGGCCGGCGCGGGC
NM_005604.3       GTTTGGTTTTGTAACAGGAGACAGAAAGAGAAAAGGATGACCCCTCCCGGAGGG------
NM_002699.3       GTCTGGTTCTGCAACCGGCGGCAGAAGGAGAAGCGCATGACCCCTGCGGCCGGCGCGGGC
                  ** ***** ** ***.**.*.*****.*****..* ********* * * .**       

HsCD00082889      CACCCGCCCATGGACGATGTATACGCGCCTGGGGAGCTAGGGCCTGGCGGGGGCGGCGCA
NM_005604.3       -ACTCTGCCGGGCGCCGAGGATGTGTACGGGGGGAGTAGGG--------------ACACT
NM_002699.3       CACCCGCCCATGGACGATGTATACGCGCCTGGGGAGCTAGGGCCTGGCGGGGGCGGCGCA
                   ** *  **. * .* .:* **. * .*  ****** :.**              .*.*:

HsCD00082889      TCGCCACCCTCCGCGCCCCCACCGCCCCCGCCGGCGGCGCTGCACCACCACCACCACCAC
NM_005604.3       CCACCACACCACGGGGTGCAGACGCCCGTCCAGTGA------------------------
NM_002699.3       TCGCCACCCTCCGCGCCCCCACCGCCCCCGCCGGCGGCGCTGCACCACCACCACCACCAC
                   *.****.* .** *   *...*****   *.*  .                        

HsCD00082889      ACACTGCCCGGCTCAGTGCAG---
NM_005604.3       ------------------------
NM_002699.3       ACACTGCCCGGCTCAGTGCAGTGA