Gene:
Gene Symbol:
ERN2
Gene Name:
endoplasmic reticulum to nucleus signaling 2
Original Clone ID:
FLH269674.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082893
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGAAGGGCGGGGATCGGCGAGGACTCCAGGCTGGGGTTGCAGGCCCAGCCAGGGGCG
GAGCCTTCTCCGGGTCGGGCGGGGACAGAGCGCTCCCTTGGAGGCACCCAGGGACCTGGC
CAGCCGTGCAGCTGCCCAGGCGCTATGGCGAGTGCGGTCAGGGGGTCGAGGCCGTGGCCC
CGGCTGGGGCTCCAGCTCCAGTTCGCGGCGCTGCTGCTCGGGACGCTGAGTCCACAGGTT
CATACTCTCAGGCCAGAGAACCTCCTGCTGGTGTCCACCTTGGATGGAAGTCTCCACGCA
CTAAGCAAGCAGACAGGGGACCTGAAGTGGACTCTGAGGGATGATCCCGTCATCGAAGGA
CCAATGTACGTCACAGAAATGGCCTTTCTCTCTGACCCAGCAGATGGCAGCCTGTACATC
TTGGGGACCCAAAAACAACAGGGATTAATGAAACTGCCATTCACCATCCCTGAGCTGGTT
CATGCCTCTCCCTGCCGCAGCTCTGATGGGGTCTTCTACACAGGCCGGAAGCAGGATGCC
TGGTTTGTGGTGGACCCTGAGTCAGGGGAGACCCAGATGACACTGACCACAGAGGGTCCC
TCCACCCCCCGCCTCTACATTGGCCGAACACAGTATACGGTCACCATGCATGACCCAAGA
GCCCCAGCCCTGCGCTGGAACACCACCTACCGCCGCTACTCAGCGCCCCCCATGGATGGC
TCACCTGGGAAATACATGAGCCACCTGGCGTCCTGCGGGATGGGCCTGCTGCTCACTGTG
GACCCAGGAAGCGGGACGGTGCTGTGGACACAGGACCTGGGCGTGCCTGTGATGGGCGTC
TACACCTGGCACCAGGACGGCCTGCGCCAGCTGCCGCATCTCACGCTGGCTCGAGACACT
CTGCATTTCCTCGCCCTCCGCTGGGGCCACATCCGACTGCCTGCCTCAGGCCCCCGGGAC
ACAGCCACCCTCTTCTCTACCTTGGACACCCAGCTGCTAATGACGCTGTATGTGGGGAAG
GATGAAACTGGCTTCTATGTCTCTAAAGCACTGGTCCACACAGGAGTGGCCCTGGTGCCT
CGTGGACTGACCCTGGCCCCCGCAGATGGCCCCACCACAGATGAGGTGACACTCCAAGTC
TCAGGAGAGCGAGAGGGCTCACCCAGCACTGCTGTTAGATACCCCTCAGGCAGTGTGGCC
CTCCCAAGCCAGTGGCTGCTCATTGGACACCACGAGCTACCCCCAGTCCTGCACACCACC
ATGCTGAGGGTCCATCCCACCCTGGGGAGTGGAACTGCAGAGACAAGACCTCCAGAGAAT
ACCCAGGCCCCAGCCTTCTTCTTGGAGCTATTGAGCCTGAGCCGAGAGAAACTTTGGGAC
TCCGAGCTGCATCCAGAAGAAAAAACTCCAGACTCTTACTTGGGGCTGGGACCCCAAGAC
CTGCTGGCAGCTAGCCTCACTGCTGTCCTCCTGGGAGGGTGGATTCTCTTTGTGATGAGG
CAGCAACAGCCGCAGGTGGTGGAGAAGCAGCAGGAGACCCCCCTGGCACCTGCAGACTTT
GCTCACATCTCCCAGGATGCCCAGTCCCTGCACTCGGGGGCCAGCCGGAGGAGCCAGAAG
AGGCTTCAGAGTCCCTCAAAGCAAGCCCAGCCACTCGACGACCCTGAAGCTGAGCAACTC
ACCGTAGTGGGGAAGATTTCCTTCAATCCCAAGGACGTGCTGGGCCGCGGGGCAGGCGGG
ACTTTCGTTTTCCGGGGACAGTTTGAGGGACGGGCAGTGGCTGTCAAGCGGCTCCTCCGC
GAGTGCTTTGGCCTGGTTCGGCGGGAAGTTCAACTGCTGCAGGAGTCTGACAGGCACCCC
AACGTGCTCCGCTACTTCTGCACCGAGCGGGGACCCCAGTTCCACTACATTGCCCTGGAG
CTCTGCCGGGCCTCCTTGCAGGAGTACGTAGAAAACCCGGACCTGGATCGCGGGGGTCTG
GAGCCCGAGGTCGTGCTGCAGCAGCTGATGTCTGGCCTGGCCCACCTGCACTCTTTACAC
ATAGTGCACCGGGACCTGAAGCCAGGAAATATTCTCATCACCGGGCCTGACAGCCAGGGC
CTGGGCAGAGTGGTGCTCTCAGACTTCGGCCTCTGCAAGAAGCTGCCTGCTGGCCGCTGT
AGCTTCAGCCTCCACTCCGGCATCCCCGGCACGGAAGGCTGGATGGCGCCCGAGCTTCTG
CAGCTCCTGCCACCAGACAGTCCTACCAGCGCTGTGGACATCTTCTCTGCAGGCTGCGTG
TTCTACTACGTGCTTTCTGGTGGCAGCCACCCCTTTGGAGACAGTCTTTATCGCCAGGCA
AACATCCTCACAGGGGCTCCCTGTCTGGCTCACCTGGAGGAAGAGGTCCACGACAAGGTG
GTTGCCCGGGACCTGGTTGGAGCCATGTTGAGCCCACTGCCGCAGCCACGCCCCTCTGCC
CCCCAGGTGCTGGCCCACCCCTTCTTTTGGAGCAGAGCCAAGCAACTCCAGTTCTTCCAG
GACGTCAGTGACTGGCTGGAGAAGGAGTCCGAGCAGGAGCCCCTGGTGAGGGCACTGGAG
GCGGGAGGCTGCGCAGTGGTCCGGGACAACTGGCACGAGCACATCTCCATGCCGCTGCAG
ACAGATCTGAGAAAGTTCCGGTCCTATAAGGGGACATCAGTGCGAGACCTGCTCCGTGCT
GTGAGGAACAAGAAGCACCACTACAGGGAGCTCCCAGTTGAGGTGCGACAGGCACTCGGC
CAAGTCCCTGATGGCTTCGTCCAGTACTTCACAAACCGCTTCCCACGGCTGCTCCTCCAC
ACGCACCGAGCCATGAGGAGCTGCGCCTCTGAGAGCCTCTTCCTGCCCTACTACCCGCCA
GACTCAGAGGCCAGGAGGCCATGCCCTGGGGCCACAGGGAGG
Protein sequence predicted based on nucleotide sequence
MRRAGIGEDSRLGLQAQPGAEPSPGRAGTERSLGGTQGPGQPCSCPGAMASAVRGSRPWP RLGLQLQFAALLLGTLSPQVHTLRPENLLLVSTLDGSLHALSKQTGDLKWTLRDDPVIEG PMYVTEMAFLSDPADGSLYILGTQKQQGLMKLPFTIPELVHASPCRSSDGVFYTGRKQDA WFVVDPESGETQMTLTTEGPSTPRLYIGRTQYTVTMHDPRAPALRWNTTYRRYSAPPMDG SPGKYMSHLASCGMGLLLTVDPGSGTVLWTQDLGVPVMGVYTWHQDGLRQLPHLTLARDT LHFLALRWGHIRLPASGPRDTATLFSTLDTQLLMTLYVGKDETGFYVSKALVHTGVALVP RGLTLAPADGPTTDEVTLQVSGEREGSPSTAVRYPSGSVALPSQWLLIGHHELPPVLHTT MLRVHPTLGSGTAETRPPENTQAPAFFLELLSLSREKLWDSELHPEEKTPDSYLGLGPQD LLAASLTAVLLGGWILFVMRQQQPQVVEKQQETPLAPADFAHISQDAQSLHSGASRRSQK RLQSPSKQAQPLDDPEAEQLTVVGKISFNPKDVLGRGAGGTFVFRGQFEGRAVAVKRLLR ECFGLVRREVQLLQESDRHPNVLRYFCTERGPQFHYIALELCRASLQEYVENPDLDRGGL EPEVVLQQLMSGLAHLHSLHIVHRDLKPGNILITGPDSQGLGRVVLSDFGLCKKLPAGRC SFSLHSGIPGTEGWMAPELLQLLPPDSPTSAVDIFSAGCVFYYVLSGGSHPFGDSLYRQA NILTGAPCLAHLEEEVHDKVVARDLVGAMLSPLPQPRPSAPQVLAHPFFWSRAKQLQFFQ DVSDWLEKESEQEPLVRALEAGGCAVVRDNWHEHISMPLQTDLRKFRSYKGTSVRDLLRA VRNKKHHYRELPVEVRQALGQVPDGFVQYFTNRFPRLLLHTHRAMRSCASESLFLPYYPP DSEARRPCPGATGR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
170
End on reference sequence
3094
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
