Gene:
Gene Symbol:
PARD3B
Gene Name:
par-3 family cell polarity regulator beta
Original Clone ID:
FLH269676.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082895
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAAGTGACCGTGTGCTTCGGCAGGACGGGCATCGTGGTGCCCTGCAAGGAGGGCCAG
CTGCGCGTCGGCGAGCTCACCCAGCAGGCGCTGCAGCGGTACCTGAAGACCCGGGAGAAG
GGTCCTGGTTACTGGGTGAAGATTCATCACTTAGAATATACAGATGGAGGAATCCTGGAT
CCAGATGATGTCTTGGCAGATGTTGTTGAAGATAAAGACAAGCTGATTGCTGTGTTTGAA
GAACAAGAACCACTCCACAAGATTGAGAGCCCCAGTGGAAACCCTGCAGATCGGCAGAGC
CCAGATGCTTTTGAGACAGAAGTGGCCGCCCAACTGGCCGCATTTAAGCCAATTGGTGGG
GAGATTGAAGTAACCCCTTCTGCTCTAAAACTAGGCACTCCACTGCTGGTGAGGAGAAGC
AGTGACCCAGTGCCAGGCCCACCTGCTGATACCCAGCCAAGCGCTTCACACCCTGGTGGC
CAGAGTCTGAAACTGGTTGTTCCAGATTCCACGCAGAACTTGGAAGACAGAGAAGTTTTG
AATGGTGTACAGACAGAACTACTAACTTCGCCAAGAACTAAGGACACATTGAGTGATATG
ACAAGAACAGTGGAGATTTCTGGGGAAGGAGGCCCATTGGGAATACATGTAGTGCCCTTC
TTTTCATCTCTGAGTGGAAGGATTCTAGGACTCTTCATCCGAGGCATTGAAGACAACAGC
AGGTCCAAGCGGGAGGGACTATTTCACGAAAATGAATGTATTGTAAAAATCAACAATGTG
GATCTCGTAGACAAAACCTTTGCTCAGGCTCAAGATGTCTTCCGCCAGGCAATGAAATCT
CCAAGTGTGCTCCTCCACGTGCTTCCTCCACAAAACCGTGAACAGTATGAAAAGTCAGTC
ATTGGCTCTCTTAACATTTTTGGTAATAATGATGGCGTTTTGAAAACCAAAGTGCCGCCT
CCTGTCCATGGAAAATCGGGACTAAAGACAGCAAATCTCACAGGAACCGATAGTCCTGAA
ACAGATGCATCAGCTTCCCTGCAACAAAACAAGAGTCCCCGAGTACCAAGGCTGGGAGGA
AAACCATCCTCTCCCTCACTCTCGCCTCTCATGGGATTTGGCAGCAATAAAAATGCAAAG
AAAATTAAGATTGACCTAAAGAAAGGCCCTGAAGGACTTGGTTTCACTGTGGTTACCAGA
GACTCTTCCATACATGGTCCCGGTCCCATTTTTGTAAAAAACATTTTACCAAAGGGAGCA
GCAATAAAAGATGGCCGCCTACAATCAGGGGACAGAATTTTGGAGGTAAATGGGAGAGAT
GTCACCGGACGAACCCAGGAAGAGCTTGTGGCCATGCTCAGGAGCACCAAGCAGGGGGAG
ACAGCATCGCTGGTCATTGCCCGCCAAGAAGGACATTTTCTGCCCCGAGAGTTGAAAGGA
GAACCTGACTGCTGTGCACTCTCTCTGGAGACAAGCGAGCAGCTCACCTTTGAGATCCCC
CTGAATGATTCAGGTTCTGCTGGCCTCGGGGTGAGCTTAAAAGGGAACAAATCCAGAGAA
ACTGGAACAGACTTGGGGATTTTTATCAAATCCATCATTCATGGAGGCGCTGCTTTTAAG
GATGGTCGTCTGCGAATGAATGACCAGCTGATTGCAGTTAATGGGGAATCTCTTTTGGGA
AAGTCCAACCACGAAGCTATGGAAACACTTAGGCGGTCAATGTCCATGGAGGGAAACATC
CGAGGGATGATCCAGTTGGTGATTCTGAGGAGGCCAGAGAGACCAATGGAGGATCCTGCA
GAGTGTGGGGCATTTTCCAAGCCATGCTTTGAGAACTGTCAAAATGCTGTAACCACCTCT
AGGCGAAATGATAATAGTATCCTGCATCCACTTGGCACTTGCAGTCCACAAGACAAACAG
AAAGGTCTATTGCTGCCCAATGACGGATGGGCCGAGAGTGAAGTTCCACCTTCTCCAACA
CCACATTCTGCTCTGGGATTGGGCCTCGAAGATTACAGCCACAGCTCTGGGGTGGATTCA
GCAGTATATTTTCCAGATCAGCACATCAACTTCAGATCTGTGACACCGGCCAGGCAGCCT
GAATCAATTAATTTGAAAGCCTCGAAGAGCATGGACCTTGTGCCAGATGAAAGCAAGGTT
CACTCATTGGCTGGACAAAAATCGGAATCTCCAAGCAAAGATTTTGGTCCAACTCTGGGT
TTGAAAAAGTCCAGCTCCTTGGAGAGTCTGCAGACTGCAGTGGCCGAGGTCAGGAAGAAT
GACCTTCCCTTTCACAGGCCCCGGCCGCACATGGTTCGAGGCCGAGGCTGCAATGAGAGC
TTTAGAGCAGCCATTGACAAATCCTACGATGGACCTGAAGAAATAGAAGCTGACGGTCTG
TCTGATAAGAGCTCTCACTCTGGCCAAGGAGCTCTGAATTGTGAGTCTGCCCCTCAGGGG
AATTCGGAGCTAGAGGACATGGAAAATAAAGCCAGGAAAGTCAAAAAAACGAAAGAGAAG
GAGAAGAAAAAGGAAAAGGGCAAATTGAAAGTCAAGGAGAAAAAGCGCAAAGAGGAGAAT
GAAGATCCAGAAAGGAAAATAAAGAAGAAGGGCTTCGGCGCCATGCTGAGATTTGGAAAG
AAGAAAGAGGATAAGGGTGGAAAGGCTGAGCAGAAAGGTACTCTGAAACATGGTGGCCTG
AGAGAAGAAGAGCTGGAGAAAATGAAAGAAGAGCGTGAAAGTGGCCGTCCTACGGGTGGA
AGCACTGACCGTATCCAGAAGTTGCGGAAAGAGTATTATCAGGCTCGGAGGGAAGGTTTC
CCTTTATATGAAGACGACGAAGGAAGAGCAAGGCCATCTGAGTATGACCTACTCTGGGTG
CCTGGAAGGGGTCCAGATGGGAATGCACACAACCTCCGCTTTGAAGGGATGGAGAGGCAG
TACGCATCCTTACCCAGGGGAGGACCCGCAGATCCTGTAGACTATCTGCCAGCAGCACCT
CGGGGGCTCTACAAGGAAAGGGAGCTTCCCTATTATCCAGGGGCTCATCCTATGCACCCT
CCCAAAGGGAGCTATCCCCGCCCCACAGAGCTCAGGGTGGCAGATCTCCGGTATCCTCAG
CACTACCCACCCCCGCCAGCTCCCCAGCACAAAGGACCCTTTCGACAAGACGTTCCGCCT
TCCCCTCCCCAGCACCAAAGAATGCCAGCCTATCAGGAAACAGGCAGACCAGGGCCCCGT
GGGGGCAGCCCAGACCAGTACCCTTACCGAACCCAGGATTCCCGGCAGAAGAACCCCATG
ACTGCAGCCGTA
Protein sequence predicted based on nucleotide sequence
MKVTVCFGRTGIVVPCKEGQLRVGELTQQALQRYLKTREKGPGYWVKIHHLEYTDGGILD PDDVLADVVEDKDKLIAVFEEQEPLHKIESPSGNPADRQSPDAFETEVAAQLAAFKPIGG EIEVTPSALKLGTPLLVRRSSDPVPGPPADTQPSASHPGGQSLKLVVPDSTQNLEDREVL NGVQTELLTSPRTKDTLSDMTRTVEISGEGGPLGIHVVPFFSSLSGRILGLFIRGIEDNS RSKREGLFHENECIVKINNVDLVDKTFAQAQDVFRQAMKSPSVLLHVLPPQNREQYEKSV IGSLNIFGNNDGVLKTKVPPPVHGKSGLKTANLTGTDSPETDASASLQQNKSPRVPRLGG KPSSPSLSPLMGFGSNKNAKKIKIDLKKGPEGLGFTVVTRDSSIHGPGPIFVKNILPKGA AIKDGRLQSGDRILEVNGRDVTGRTQEELVAMLRSTKQGETASLVIARQEGHFLPRELKG EPDCCALSLETSEQLTFEIPLNDSGSAGLGVSLKGNKSRETGTDLGIFIKSIIHGGAAFK DGRLRMNDQLIAVNGESLLGKSNHEAMETLRRSMSMEGNIRGMIQLVILRRPERPMEDPA ECGAFSKPCFENCQNAVTTSRRNDNSILHPLGTCSPQDKQKGLLLPNDGWAESEVPPSPT PHSALGLGLEDYSHSSGVDSAVYFPDQHINFRSVTPARQPESINLKASKSMDLVPDESKV HSLAGQKSESPSKDFGPTLGLKKSSSLESLQTAVAEVRKNDLPFHRPRPHMVRGRGCNES FRAAIDKSYDGPEEIEADGLSDKSSHSGQGALNCESAPQGNSELEDMENKARKVKKTKEK EKKKEKGKLKVKEKKRKEENEDPERKIKKKGFGAMLRFGKKKEDKGGKAEQKGTLKHGGL REEELEKMKEERESGRPTGGSTDRIQKLRKEYYQARREGFPLYEDDEGRARPSEYDLLWV PGRGPDGNAHNLRFEGMERQYASLPRGGPADPVDYLPAAPRGLYKERELPYYPGAHPMHP PKGSYPRPTELRVADLRYPQHYPPPPAPQHKGPFRQDVPPSPPQHQRMPAYQETGRPGPR GGSPDQYPYRTQDSRQKNPMTAAV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
208
End on reference sequence
3522
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
