Gene:
Gene Symbol:
GPR113
Gene Name:
G protein-coupled receptor 113
Original Clone ID:
FLH269811.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082934
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGTCTGTTCGGCTGCCCCACTGCTGCTCCTGGCCACAACTCTTCCCCTGCTGGGGTCA
CCAGTTGCCCAAGCATCCCAACCTGTAAGTGAGACTGGGGTGAGACCCAGGGAAGGTCTG
CAGAGGCGACAATGGGGACCCCTGATTGGGAGAGACAAAGCATGGAATGAAAGGATAGAC
AGACCCTTCCCTGCCTGCCCCATCCCCCTATCTTCTAGCTTTGGCCGATGGCCCAAGGGC
CAGACAATGTGGGCCCAGACCTCCACCCTCACCCTGACAGAGGAGGAGTTGGGACAGAGT
CAGGCTGGAGGGGAATCTGGATCTGGGCAGCTCCTGGACCAAGAGAATGGAGCAGGGGAA
TCAGCGCTGGTCTCCGTCTATGTACATCTGGACTTTCCAGATAAGACCTGGCCCCCTGAA
CTCTCCAGGACACTGACTCTCCCTGCTGCCTCAGCTTCCTCTTCCCCAAGGCCTCTTCTC
ACTGGCCTCAGACTCACAACAGAGTGTAATGTCAACCACAAGGGGAATTTCTATTGTGCT
TGCCTCTCTGGCTACCAGTGGAACACCAGCATCTGCCTCCATTACCCTCCTTGTCAAAGC
CTCCACAACCACCAGCCTTGTGGCTGCCTTGTCTTCAGCCATCCCGAACCCGGGTACTGC
CAGTTGCTGCCACCTGGGTCCCCTGTCACCTGCCTCCCTGCAGTCCCCGGGATCCTCAAC
CTGAACTCCCAGCTGCAGATGCCTGGTGACACGCTGAGCCTGACTCTCCATCTGAGCCAG
GAGGCCACCAACCTGAGCTGGTTCCTGAGGCACCCAGGGAGCCCCAGTCCCATCCTCCTG
CAGCCAGGGACACAGGTGTCTGTGACTTCCAGCCACGGCCAGGCTGCCCTCAGCGTCTCC
AACATGTCCCATCACTGGGCAGGTGAGTACATGAGCTGCTTCGAGGCCCAGGGCTTCAAG
TGGAACCTGTATGAGGTGGTGAGGGTGCCCTTGAAGGCGACAGATGTGGCTCGACTTCCA
TACCAGCTGTCCATCTCCTGTGCCACCTCCCCTGGCTTCCAGCTGAGCTGCTGCATCCCC
AGCACAAACCTGGCCTACACCGCGGCCTGGAGCCCTGGAGAGGGCAGCAAAGCTTCCTCC
TTCAACGAGTCAGGCTCTCAGTGCTTTGTGCTGGCTGTTCAGCGCTGCCCGATGGCTGAC
ACCACGTACGCTTGTGACCTGCAGAGCCTGGGCCTGGCTCCACTCAGGGTCCCCATCTCC
ATCACCATCATCCAGGATGGAGACATCACCTGCCCTGAGGACGCCTCGGTGCTCACCTGG
AATGTCACCAAGGCTGGCCACGTGGCACAGGCCCCATGTCCTGAGAGCAAGAGGGGCATA
GTGAGGAGGCTCTGTGGGGCTGACGGAGTCTGGGGGCCGGTCCACAGCAGCTGCACAGAT
GCGAGGCTCCTGGCCTTGTTCACTAGAACCAAGCTGCTGCAGGCAGGCCAGGGCAGTCCT
GCTGAGGAGGTGCCACAGATCCTGGCACAGCTGCCAGGGCAGGCGGCAGAGGCAAGTTCA
CCCTCCGACTTACTGACCCTGCTGAGCACCATGAAATACGTGGCCAAGGTGGTGGCAGAG
GCCAGAATACAGCTTGACCGCAGAGCCCTGAAGAATCTCCTGATTGCCACAGACAAGGTC
CTAGATATGGACACCAGGTCTCTGTGGACCCTGGCCCAAGCCCGGAAGCCCTGGGCAGGC
TCGACTCTCCTGCTGGCTGTGGAGACCCTGGCATGCAGCCTGTGCCCACAGGACCACCCC
TTCGCCTTCAGCTTACCCAATGTGCTGCTGCAGAGCCAGCTGTTTGGACCCACGTTTCCT
GCTGACTACAGCATCTCCTTCCCTACTCGGCCCCCACTGCAGGCTCAGATTCCCAGGCAC
TCACTGGCCCCATTGGTCCGTAATGGAACTGAAATAAGTATTACTAGCCTGGTGCTGCGA
AAACTGGACCACCTTCTGCCCTCAAACTATGGACAAGGGCTGGGGGATTCCCTCTATGCC
ACTCCTGGCCTGGTCCTTGTCATTTCCATCATGGCAGGTGACCGGGCCTTCAGCCAGGGA
GAGGTCATCATGGACTTTGGGAACACAGATGGTTCCCCTCACTGTGTCTTCTGGGATCAC
AGTCTCTTCCAGGGCAGGGGGGGTTGGTCCAAAGAAGGGTGCCAGGCACAGGTGGCCAGT
GCCAGCCCCACTGCTCAGTGCCTCTGCCAGCACCTCACTGCCTTCTCCGTCCTCATGTCC
CCACACACTGTTCCGGAAGAACCCGCTCTGGCGCTGCTGACTCAAGTGGGCTTGGGAGCT
TCCATACTGGCGCTGCTTGTGTGCCTGGGTGTGTACTGGCTGGTGTGGAGAGTCGTGGTG
CGGAACAAGATCTCCTATTTCCGCCACGCCGCCCTGCTCAACATGGTGTTCTGCTTGCTG
GCCGCAGACACTTGCTTCCTGGGCGCCCCATTCCTCTCTCCAGGGCCCCGAAGCCCGCTC
TGCCTTGCTGCCGCCTTCCTCTGTCATTTCCTCTACCTGGCCACCTTTTTCTGGATGCTG
GCGCAGGCCCTGGTGTTGGCCCACCAGCTGCTCTTTGTCTTTCACCAGCTGGCAAAGCAC
CGAGTTCTCCCCCTCATGGTGCTCCTGGGCTACCTGTGCCCACTGGGGTTGGCAGGTGTC
ACCCTGGGGCTCTACCTACCTCAAGGGCAATACCTGAGGGAGGGGGAATGCTGGTTGGAT
GGGAAGGGAGGGGCGTTATACACCTTCGTGGGGCCAGTGCTGGCCATCATAGGCGTGAAT
GGGCTGGTACTAGCCATGGCCATGCTGAAGTTGCTGAGACCTTCGCTGTCAGAGGGACCC
CCAGCAGAGAAGCGCCAAGCTCTGCTGGGGGTGATCAAAGCCCTGCTCATTCTTACACCC
ATCTTTGGCCTCACCTGGGGGCTGGGCCTGGCCACTCTGTTAGAGGAAGTCTCCACGGTC
CCTCATTACATCTTCACCATTCTCAACACCCTCCAGGGCGTCTTCATCCTATTGTTTGGT
TGCCTCATGGACAGGAAGATACAAGAAGCTTTGCGCAAACGCTTCTGCCGCGCCCAAGCC
CCCAGCTCCACCATCTCCCTGGTGAGTTGCTGCCTTCAGATCCTCAGCTGTGCATCCAAG
AGCATGTCAGAAGGCATTCCATGGCCCTCCTCAGAGGACATGGGCACAGCCAGAAGCTAA
Protein sequence predicted based on nucleotide sequence
MVCSAAPLLLLATTLPLLGSPVAQASQPVSETGVRPREGLQRRQWGPLIGRDKAWNERID RPFPACPIPLSSSFGRWPKGQTMWAQTSTLTLTEEELGQSQAGGESGSGQLLDQENGAGE SALVSVYVHLDFPDKTWPPELSRTLTLPAASASSSPRPLLTGLRLTTECNVNHKGNFYCA CLSGYQWNTSICLHYPPCQSLHNHQPCGCLVFSHPEPGYCQLLPPGSPVTCLPAVPGILN LNSQLQMPGDTLSLTLHLSQEATNLSWFLRHPGSPSPILLQPGTQVSVTSSHGQAALSVS NMSHHWAGEYMSCFEAQGFKWNLYEVVRVPLKATDVARLPYQLSISCATSPGFQLSCCIP STNLAYTAAWSPGEGSKASSFNESGSQCFVLAVQRCPMADTTYACDLQSLGLAPLRVPIS ITIIQDGDITCPEDASVLTWNVTKAGHVAQAPCPESKRGIVRRLCGADGVWGPVHSSCTD ARLLALFTRTKLLQAGQGSPAEEVPQILAQLPGQAAEASSPSDLLTLLSTMKYVAKVVAE ARIQLDRRALKNLLIATDKVLDMDTRSLWTLAQARKPWAGSTLLLAVETLACSLCPQDHP FAFSLPNVLLQSQLFGPTFPADYSISFPTRPPLQAQIPRHSLAPLVRNGTEISITSLVLR KLDHLLPSNYGQGLGDSLYATPGLVLVISIMAGDRAFSQGEVIMDFGNTDGSPHCVFWDH SLFQGRGGWSKEGCQAQVASASPTAQCLCQHLTAFSVLMSPHTVPEEPALALLTQVGLGA SILALLVCLGVYWLVWRVVVRNKISYFRHAALLNMVFCLLAADTCFLGAPFLSPGPRSPL CLAAAFLCHFLYLATFFWMLAQALVLAHQLLFVFHQLAKHRVLPLMVLLGYLCPLGLAGV TLGLYLPQGQYLREGECWLDGKGGALYTFVGPVLAIIGVNGLVLAMAMLKLLRPSLSEGP PAEKRQALLGVIKALLILTPIFGLTWGLGLATLLEEVSTVPHYIFTILNTLQGVFILLFG CLMDRKIQEALRKRFCRAQAPSSTISLVSCCLQILSCASKSMSEGIPWPSSEDMGTARS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3240
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
