Gene:
Gene Symbol:
CHD5
Gene Name:
chromodomain helicase DNA binding protein 5
Original Clone ID:
FLH269825.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082948
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCGGGGCCCAGTGGGCACCGAGGAGGAGCTGCCGCGGCTGTTCGCCGAGGAGATGGAG
AATGAGGACGAGATGTCAGAAGAAGAAGATGGTGGTCTTGAAGCCTTCGATGACTTTTTC
CCTGTGGAGCCCGTGAGCCTTCCTAAGAAGAAGAAACCCAAGAAGCTCAAGGAAAACAAG
TGTAAAGGGAAGCGGAAGAAGAAAGAGGGGAGCAATGATGAGCTATCAGAGAATGAAGAG
GATCTGGAAGAGAAGTCGGAGAGTGAAGGCAGTGACTACTCCCCGAATAAAAAGAAGAAG
AAGAAACTCAAGGACAAGAAGGAGAAAAAAGCCAAGCGAAAAAAGAAGGATGAGGATGAG
GATGATAATGATGATGGATGCTTAAAGGAGCCCAAGTCCTCGGGGCAGCTCATGGCCGAG
TGGGGCCTGGACGACGTGGACTACCTGTTCTCGGAGGAGGATTACCACACGCTGACCAAC
TACAAGGCCTTCAGCCAGTTCCTCAGGCCACTCATTGCCAAGAAGAACCCGAAGATCCCC
ATGTCCAAAATGATGACCGTCCTGGGTGCCAAGTGGCGGGAGTTCAGCGCCAACAACCCC
TTCAAGGGCAGCTCCGCGGCAGCAGCGGCGGCGGCGGTGGCTGCGGCTGTAGAGACGGTC
ACCATCTCCCCTCCGCTAGCCGTCAGCCCCCCGCAGGTGCCCCAGCCTGTGCCTATCCGC
AAGGCCAAGACCAAGGAGGGCAAAGGGCCTGGAGTGAGGAAGAAGATCAAAGGCTCCAAA
GATGGGAAGAAAAAGGGCAAAGGGAAAAAGACGGCCGGGCTCAAGTTCCGCTTCGGGGGG
ATCAGCAACAAGAGGAAGAAAGGCTCCTCGAGTGAAGAAGATGAGAGGGAGGAGTCGGAC
TTCGACAGCGCCAGCATCCACAGTGCCTCCGTGCGCTCCGAATGCTCTGCAGCCCTGGGC
AAGAAGAGCAAGAGGAGGCGCAAGAAGAAGAGGATTGATGATGGTGACGGCTATGAGACA
GACCACCAGGATTACTGTGAGGTGTGCCAGCAGGGTGGGGAGATCATCCTGTGCGACACC
TGCCCGAGGGCCTACCATCTCGTATGCCTGGACCCAGAGCTGGAGAAGGCTCCCGAGGGC
AAGTGGAGCTGCCCCCACTGTGAGAAGGAGGGGATCCAGTGGGAGCCGAAGGACGACGAC
GATGAAGAGGAGGAGGGCGGCTGCGAGGAGGAGGAGGACGACCACATGGAGTTCTGCCGC
GTGTGCAAGGACGGGGGCGAGCTGCTCTGCTGCGACGCCTGCCCCTCCTCCTACCACCTG
CATTGCCTCAACCCGCCGCTGCCCGAGATCCCAAACGGTGAATGGCTCTGCCCGCGCTGT
ACTTGCCCCCCACTGAAGGGCAAAGTCCAGCGGATTCTACACTGGAGGTGGACGGAGCCC
CCTGCCCCCTTCATGGTGGGGCTGCCGGGGCCTGACGTGGAGCCCAGCCTCCCTCCACCT
AAGCCCCTGGAGGGCATCCCTGAGAGAGAGTTCTTTGTCAAGTGGGCAGGGCTGTCCTAC
TGGCATTGCTCCTGGGTGAAGGAGCTACAGCTGGAGCTGTACCACACGGTGATGTATCGC
AACTACCAAAGAAAGAACGACATGGATGAGCCGCCCCCCTTTGACTACGGCTCTGGGGAT
GAAGACGGCAAGAGCGAGAAGAGGAAGAACAAGGACCCCCTCTATGCCAAGATGGAGGAG
CGCTTCTACCGCTATGGCATCAAGCCAGAGTGGATGATGATTCACCGAATCCTGAACCAT
AGCTTTGACAAGAAGGGGGATGTGCACTACCTGATCAAGTGGAAAGACCTGCCCTACGAC
CAGTGCACCTGGGAGATCGATGACATCGACATCCCCTACTACGACAACCTCAAGCAGGCC
TACTGGGGCCACAGGGAGCTGATGCTGGGAGAAGACACCAGGCTGCCCAAGAGGCTGCTC
AAGAAGGGCAAGAAGCTGAGGGACGACAAGCAGGAGAAGCCGCCGGACACGCCCATTGTG
GACCCCACGGTCAAGTTCGACAAGCAGCCATGGTACATCGACTCCACAGGCGGCACACTG
CACCCGTACCAGCTGGAGGGCCTCAACTGGCTGCGCTTCTCTTGGGCCCAGGGCACTGAC
ACCATCCTGGCCGATGAGATGGGTCTGGGCAAGACGGTGCAGACCATCGTGTTCCTTTAC
TCCCTCTACAAGGAGGGCCACTCCAAAGGGCCCTACCTGGTTAGCGCGCCCCTCTCCACC
ATCATCAACTGGGAACGCGAGTTTGAGATGTGGGCGCCCGACTTCTACGTGGTCACCTAC
ACGGGGGACAAGGAGAGCCGCTCGGTGATTCGGGAGAACGAGTTTTCCTTTGAGGACAAC
GCCATTCGGAGTGGGAAGAAGGTATTCCGTATGAAGAAAGAAGTGCAGATCAAATTCCAC
GTGCTGCTCACCTCCTATGAGCTCATCACCATTGACCAGGCCATCCTGGGCTCCATCGAG
TGGGCCTGCCTGGTGGTAGATGAGGCCCACCGCCTCAAGAACAACCAGTCCAAGTTTTTT
AGGGTCTTAAACAGCTACAAGATTGATTACAAGCTGCTGCTGACAGGGACCCCCCTTCAG
AACAACCTGGAGGAGCTGTTCCATCTCCTCAACTTCCTGACTCCAGAGAGGTTCAACAAC
CTGGAGGGCTTCCTGGAGGAGTTTGCTGACATCTCCAAGGAAGACCAGATCAAGAAGCTG
CATGACCTGCTGGGGCCGCACATGCTCAGGCGGCTCAAGGCTGACGTGTTCAAGAACATG
CCGGCCAAGACCGAGCTCATTGTCCGGGTGGAGCTGAGCCAGATGCAGAAGAAGTACTAC
AAGTTCATCCTCACACGGAACTTTGAGGCACTGAACTCCAAGGGGGGCGGGAACCAAGTA
TCGCTGCTCAACATCATGATGGACCTGAAAAAGTGCTGCAACCACCCCTACCTCTTCCCT
GTGGCTGCCGTGGAGGCCCCTGTCTTGCCCAATGGCTCCTACGATGGAAGCTCCCTGGTC
AAGTCTTCAGGGAAGCTCATGCTGCTACAGAAGATGCTGAAGAAACTGCGGGATGAGGGG
CACCGTGTGCTCATCTTCTCCCAGATGACCAAGATGCTGGACCTCCTGGAGGACTTCCTG
GAGTACGAAGGCTACAAGTATGAGCGGATTGATGGTGGCATCACCGGGGGCCTCCGGCAG
GAGGCAATCGACAGATTCAATGCCCCCGGGGCCCAGCAGTTCTGCTTCCTCCTCTCAACC
CGGGCAGGTGGTCTGGGCATCAACCTGGCCACGGCGGACACTGTCATCATCTACGACTCG
GACTGGAACCCGCACAATGACATCCAGGCCTTCAGCCGCGCCCACCGCATCGGCCAGAAC
AAGAAGGTGATGATCTACCGCTTCGTGACTCGGGCCTCGGTGGAGGAGCGCATCACGCAG
GTGGCCAAGCGCAAGATGATGCTCACCCACCTGGTGGTGCGGCCCGGCCTCGGCTCCAAG
TCGGGGTCCATGACCAAGCAGGAGCTGGACGACATCCTCAAGTTCGGCACGGAGGAACTC
TTCAAGGACGACGTGGAGGGCATGATGTCTCAGGGCCAGAGGCCGGTCACACCCATCCCT
GATGTCCAGTCCTCCAAAGGGGGGAACTTGGCCGCCAGTGCAAAGAAGAAGCACGGTAGC
ACCCCGCCAGGTGACAACAAGGACGTGGAGGACAGCAGTGTGATCCACTATGACGATGCG
GCCATCTCCAAGCTGCTGGACCGGAACCAGGACGCTACAGATGACACGGAGCTACAGAAC
ATGAACGAGTACCTGAGCTCCTTCAAGGTGGCGCAGTACGTGGTGCGCGAGGAGGACGGC
GTGGAGGAGGTGGAGCGGGAAATCATCAAGCAGGAGGAGAACGTGGACCCCGACTACTGG
GAGAAGCTGCTGCGGCACCACTATGAGCAGCAGCAGGAGGACCTGGCCCGCAACCTGGGC
AAGGGCAAGCGCATCCGCAAGCAGGTCAACTACAACGATGCCTCCCAGGAGGACCAGGAG
TGGCAGGATGAGCTCTCTGATAACCAGTCAGAATATTCCATTGGCTCTGAGGATGAGGAT
GAGGACTTTGAAGAGAGGCCGGAAGGGCAGAGTGGACGACGACAATCCCGGAGGCAGCTG
AAGAGTGACAGGGACAAGCCCCTGCCCCCGCTTCTCGCCCGAGTTGGTGGCAACATCGAG
GTGCTGGGCTTCAATGCCCGACAGCGGAAGGCCTTTCTGAACGCCATCATGCGCTGGGGC
ATGCCCCCGCAGGACGCCTTCAACTCCCACTGGCTGGTGCGGGACCTTCGAGGGAAGAGC
GAGAAGGAGTTTAGAGCCTATGTGTCCCTCTTCATGCGGCACCTGTGTGAGCCGGGGGCG
GATGGTGCAGAGACCTTCGCAGACGGCGTGCCCCGGGAGGGCCTCTCCAGGCAGCACGTG
CTGACCCGCATCGGGGTCATGTCACTAGTTAGGAAGAAGGTTCAGGAGTTTGAGCATGTC
AACGGGAAGTACAGCACCCCAGACTTGATCCCTGAGGGGCCCGAGGGGAAGAAGTCGGGC
GAGGTGATCTCCTCGGACCCCAACACACCAGTGCCCGCCAGCCCTGCCCACCTCCTGCCA
GCCCCGCTGGGCCTGCCAGACAAAATGGAAGCCCAGCTGGGCTACATGGATGAGAAAGAC
CCCGGGGCACAGAAGCCAAGGCAGCCCCTGGAAGTCCAGGCCCTTCCAGCCGCCTTGGAT
AGAGTGGAGAGTGAGGACAAGCACGAGAGCCCAGCCAGCAAGGAGAGAGCCCGAGAGGAG
CGGCCAGAGGAGACGGAGAAGGCCCCGCCCTCCCCGGAGCAGCTGCCGAGAGAGGAGGTG
CTTCCTGAGAAGGAGAAGATCCTGGACAAGCTGGAGCTGAGCTTGATCCACAGCAGAGGG
GACAGTTCCGAACTCAGGCCAGATGACACCAAGGCTGAGGAGAAGGAGCCCATTGAAACA
CAGCAAAATGGTGACAAAGAGGAAGATGACGAGGGGAAGAAGGAGGACAAGAAGGGGAAA
TTCAAGTTCATGTTCAACATCGCGGACGGGGGCTTCACGGAGTTGCACACGCTGTGGCAG
AACGAGGAGCGGGCTGCTGTATCCTCTGGGAAAATCTACGACATCTGGCACCGGCGCCAT
GACTACTGGCTGCTGGCGGGCATCGTGACGCACGGCTACGCCCGCTGGCAGGACATCCAG
AATGACCCACGGTACATGATCCTCAACGAGCCCTTCAAGTCTGAGGTCCACAAGGGCAAC
TACCTGGAGATGAAGAACAAGTTCCTGGCCCGCAGGTTTAAGCTGCTGGAGCAGGCGTTG
GTCATTGAGGAGCAGCTCCGGAGGGCCGCGTACCTGAACATGACGCAGGACCCCAACCAC
CCCGCCATGGCCCTCAACGCCCGCCTGGCTGAAGTGGAGTGCCTCGCCGAGAGCCACCAG
CACCTGTCCAAGGAGTCCCTTGCTGGGAACAAGCCTGCCAATGCCGTCCTGCACAAGGTC
CTGAACCAGCTGGAGGAGCTGCTGAGCGACATGAAGGCCGACGTGACCCGGCTGCCATCC
ATGCTGTCCCGCATCCCCCCGGTGGCCGCCCGGCTGCAGATGTCGGAGCGCAGCATCCTG
AGCCGCCTGACCAACCGCGCCGGGGACCCCACCATCCAGCAGGGCGCTTTCGGCTCCTCC
CAGATGTACAGCAACAACTTTGGGCCCAACTTCCGGGGCCCTGGACCGGGAGGGATTGTC
AACTACAACCAGATGCCCCTGGGGCCCTATGTGACCGATATCTAA
Protein sequence predicted based on nucleotide sequence
MRGPVGTEEELPRLFAEEMENEDEMSEEEDGGLEAFDDFFPVEPVSLPKKKKPKKLKENK CKGKRKKKEGSNDELSENEEDLEEKSESEGSDYSPNKKKKKKLKDKKEKKAKRKKKDEDE DDNDDGCLKEPKSSGQLMAEWGLDDVDYLFSEEDYHTLTNYKAFSQFLRPLIAKKNPKIP MSKMMTVLGAKWREFSANNPFKGSSAAAAAAAVAAAVETVTISPPLAVSPPQVPQPVPIR KAKTKEGKGPGVRKKIKGSKDGKKKGKGKKTAGLKFRFGGISNKRKKGSSSEEDEREESD FDSASIHSASVRSECSAALGKKSKRRRKKKRIDDGDGYETDHQDYCEVCQQGGEIILCDT CPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEPKDDDDEEEEGGCEEEEDDHMEFCR VCKDGGELLCCDACPSSYHLHCLNPPLPEIPNGEWLCPRCTCPPLKGKVQRILHWRWTEP PAPFMVGLPGPDVEPSLPPPKPLEGIPEREFFVKWAGLSYWHCSWVKELQLELYHTVMYR NYQRKNDMDEPPPFDYGSGDEDGKSEKRKNKDPLYAKMEERFYRYGIKPEWMMIHRILNH SFDKKGDVHYLIKWKDLPYDQCTWEIDDIDIPYYDNLKQAYWGHRELMLGEDTRLPKRLL KKGKKLRDDKQEKPPDTPIVDPTVKFDKQPWYIDSTGGTLHPYQLEGLNWLRFSWAQGTD TILADEMGLGKTVQTIVFLYSLYKEGHSKGPYLVSAPLSTIINWEREFEMWAPDFYVVTY TGDKESRSVIRENEFSFEDNAIRSGKKVFRMKKEVQIKFHVLLTSYELITIDQAILGSIE WACLVVDEAHRLKNNQSKFFRVLNSYKIDYKLLLTGTPLQNNLEELFHLLNFLTPERFNN LEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSQMQKKYY KFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGSSLV KSSGKLMLLQKMLKKLRDEGHRVLIFSQMTKMLDLLEDFLEYEGYKYERIDGGITGGLRQ EAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQN KKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKSGSMTKQELDDILKFGTEEL FKDDVEGMMSQGQRPVTPIPDVQSSKGGNLAASAKKKHGSTPPGDNKDVEDSSVIHYDDA AISKLLDRNQDATDDTELQNMNEYLSSFKVAQYVVREEDGVEEVEREIIKQEENVDPDYW EKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDELSDNQSEYSIGSEDED EDFEERPEGQSGRRQSRRQLKSDRDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRWG MPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHV LTRIGVMSLVRKKVQEFEHVNGKYSTPDLIPEGPEGKKSGEVISSDPNTPVPASPAHLLP APLGLPDKMEAQLGYMDEKDPGAQKPRQPLEVQALPAALDRVESEDKHESPASKERAREE RPEETEKAPPSPEQLPREEVLPEKEKILDKLELSLIHSRGDSSELRPDDTKAEEKEPIET QQNGDKEEDDEGKKEDKKGKFKFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRH DYWLLAGIVTHGYARWQDIQNDPRYMILNEPFKSEVHKGNYLEMKNKFLARRFKLLEQAL VIEEQLRRAAYLNMTQDPNHPAMALNARLAEVECLAESHQHLSKESLAGNKPANAVLHKV LNQLEELLSDMKADVTRLPSMLSRIPPVAARLQMSERSILSRLTNRAGDPTIQQGAFGSS QMYSNNFGPNFRGPGPGGIVNYNQMPLGPYVTDI*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
101
End on reference sequence
5965
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
