Gene:
Gene Symbol:
PCDH11X
Gene Name:
protocadherin 11 X-linked
Original Clone ID:
FLH270003.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00083030
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTTTAGGGTTGGCTTCTTAATAATTTCTTCTTCCTCTTCTCTCTCTCCTCTTCTTTTG
GTCAGTGTTGTGCGGGTTAATACAACAAACTGTCACAAGTGTTTGTTGTCCGGGACGTAC
ATTTTCGCGGTCCTGCTAGTATGCGTGGTGTTCCACTCTGGCGCCCAGGAGAAAAACTAC
ACCATCCGAGAAGAAATTCCAGAAAACGTCCTGATAGGCAACTTGTTGAAAGACCTTAAC
TTGTCGCTGATTCCAAACAAGTCCTTGACAACTACTATGCAGTTCAAGCTAGTGTACAAG
ACCGGAGATGTGCCACTGATTCGAATTGAAGAGGATACTGGTGAGATCTTCACTACCGGC
GCTCGCATTGATCGTGAGAAATTATGTGCTGGTATCCCAAGGGATGAGCATTGCTTTTAT
GAAGTGGAGGTTGCCATTTTGCCGGATGAAATATTTAGACTGGTTAAGATACGTTTTCTG
ATAGAAGATATAAATGATAATGCACCATTGTTCCCAGCAACAGTTATCAACATATCAATT
CCAGAGAACTCGGCTATAAACTCTAAATATACTCTCCCAGCGGCTGTTGATCCTGACGTA
GGCATAAACGGAGTTCAAAACTACGAACTAATTAAGAGTCAAAACATTTTTGGCCTCGAT
GTCATTGAAACACCAGAAGGAGACAAGATGCCACAACTGATTGTTCAAAAGGAGTTAGAT
AGGGAAGAGAAGGATACCTATGTGATGAAAGTAAAGGTTGAAGATGGTGGCTTTCCTCAA
AGATCCAGTACTGCTATTTTGCAAGTAAGTGTTACTGATACAAATGACAACCACCCAGTC
TTTAAGGAGACAGAGATTGAAGTCAGTATACCAGAAAATGCTCCTGTAGGCACTTCAGTG
ACACAGCTCCATGCCACAGATGCTGACATAGGTGAAAATGCCAAGATCCACTTCTCTTTC
AGCAATCTAGTCTCCAACATTGCCAGGAGATTATTTCACCTCAATGCCACCACTGGACTT
ATCACAATCAAAGAACCACTGGATAGGGAAGAAACACCAAACCACAAGTTACTGGTTTTG
GCAAGTGATGGTGGATTGATGCCAGCAAGAGCAATGGTGCTGGTAAATGTTACAGATGTC
AATGATAATGTCCCATCCATTGACATAAGATACATCGTCAATCCTGTCAATGACACAGTT
GTTCTTTCAGAAAATATTCCACTCAACACCAAAATTGCTCTCATAACTGTGACGGATAAG
GATGCGGACCATAATGGCAGGGTGACATGCTTCACAGATCATGAAATTCCTTTCAGATTA
AGGCCAGTATTCAGTAATCAGTTCCTCCTGGAGAATGCAGCATATCTTGACTATGAGTCC
ACAAAAGAATATGCCATTAAATTACTGGCTGCAGATGCTGGCAAACCTCCTTTGAATCAG
TCAGCAATGCTCTTCATCAAAGTGAAAGATGAAAATGACAATGCTCCAGTTTTCACCCAG
TCTTTCGTAACTGTTTCTATTCCTGAGAATAACTCTCCTGGCATCCAGTTGATGAAAGTA
AGTGCAACGGATGCAGACAGTGGGCCTAATGCTGAGATCAATTACCTGCTAGGCCCTGAT
GCTCCACCTGAATTCAGCCTGGATCGTCGTACAGGCATGCTGACTGTAGTGAAGAAACTA
GATAGAGAAAAAGAGGATAAATATTTATTCACAATTCTGGCAAAAGATAATGGGGTACCA
CCCTTAACCAGCAATGTCACAGTCTTTGTAAGCATTATTGATCAGAATGACAATAGCCCA
GTTTTCACTCACAATGAATACAAATTCTATGTCCCAGAAAACCTTCCAAGGCATGGTACA
GTAGGACTAATCACTGTAACTGATCCTGATTATGGAGACAATTCTGCAGTTACGCTCTCC
ATTTTAGATGAGAATGATGACTTCACCATTGATTCACAAACTGGTGTCATCCGACCAAAT
ATTTCATTTGATAGAGAAAAACAAGAATCTTACACTTTCTATGTAAAGGCTGAGGATGGT
GGTAGAGTATCACGTTCTTCAAGTGCCAAAGTAACCATAAATGTGGTTGATGTCAATGAC
AACAAACCAGTTTTCATTGTCCCTCCTTACAACTATTCTTATGAATTGGTTCTACCGTCC
ACTAATCCAGGCACAGTGGTCTTTCAGGTAATTGCTGTTGACAATGACACTGGCATGAAT
GCAGAGGTTCGTTACAGCATTGTAGGAGGAAACACAAGAGATCTGTTTGCAATCGACCAA
GAAACAGGCAACATAACATTGATGGAGAAATGTGATGTTACAGACCTTGGTTTACACAGA
GTGTTGGTCAAAGCTAATGACTTAGGACAGCCTGATTCTCTCTTCAGTGTTGTAATTGTC
AATCTGTTCGTGAATGAGTCAGTGACCAATGCTACACTGATTAATGAACTGGTGCGCAAA
AGCATTGAAGCACCAGTGACCCCAAATACTGAGATAGCTGATGTATCCTCACCAACTAGT
GACTATGTCAAGATCCTGGTTGCAGCTGTTGCTGGCACCATAACTGTCGTTGTAGTTATT
TTCATCACTGCTGTAGTAAGATGTCGCCAGGCACCACACCTTAAGGCTGCTCAGAAAAAC
ATGCAGAATTCTGAATGGGCTACCCCAAACCCAGAAAACAGGCAGATGATAATGATGAAG
AAAAAGAAAAAGAAGAAGAAGCATTCCCCTAAGAACCTGCTGCTTAATGTTGTCACTATT
GAAGAAACTAAGGCAGATGATGTTGACAGTGATGGAAACAGAGTCACACTAGACCTTCCT
ATTGATCTAGAAGAGCAAACAATGGGAAAGTACAATTGGGTAACTACACCTACTACTTTC
AAGCCTGACAGCCCTGATTTGGCCCGACACTACAAATCTGCCTCTCCACAGCCTGCCTTC
CAAATTCAGCCTGAAACTCCCCTGAATTTGAAGCACCACATCATCCAAGAACTGCCTCTC
GATAACACCTTTGTGGCCTGTGACTCTATCTCCAATTGTTCCTCAAGCAGTTCAGATCCC
TACAGCGTTTCTGACTGTGGCTATCCAGTGACAACCTTCGAGGTACCTGTGTCCGTACAC
ACCAGACCGTCCCAGCGGCGTGTCACATTTCACCTGCCAGAAGGCTCTCAGGAAAGCAGC
AGTGATGGTGGACTGGGAGACCATGATGCAGGCAGCCTTACCAGCACATCCCATGGCCTG
CCCCTTGGCTATCCTCAGGAGGAGTACTTTGATCGTGCTACACCCAGCAATCGCACTGAA
GGGGATGGCAACTCCGATCCTGAATCTACTTTCATACCTGGACTAAAGAAAGAAATAACT
GTTCAACCAACTGTGGAAGAGGCCTCTGACAACTGCACTCAAGAATGTCTCATCTATGGC
CATTCTGATGCCTGCTGGATGCCGGCATCTCTGGATCATTCCAGCTCTTCACAAGCACAG
GCCTCTGCTCTATGCCACAGCCCACCACTGTCACAGGCCTCTACTCAGCACCACAGCCCA
CCAGTGACACAGACCATTGTTCTCTGCCACAGCCCTCCAGTGACACAGACCATCGCATTG
TGCCACAGCCCACCACCGATACAGGTGTCTGCTCTCCACCACAGTCCTCCTCTAGTGCAG
GGTACTGCACTTCACCACAGCCCACCATCAGCACAGGCCTCAGCCCTCTGCTACAGCCCT
CCTTTAGCACAGGCTGCTGCAATCAGCCACAGCTCTTCTCTGCCACAGGTTATTGCCCTC
CATCGTAGTCAGGCCCAATCATCAGTCAGTTTGCAGCAAGGTTGGGTGCAAGGTGCTAAT
GGACTATGCTCTGTTGATCAGGGAGTGCAAGGTAGTGCAACATCTCAGTTTTACACCATG
TCTGAAAGACTTCATCCCAGTGATGATTCAATTAAAGTCATTCCTTTGACAACCTTCGCT
CCACGCCAACAGGCCAGACCGTCCAGAGGTGATTCCCCCATTATGGAAACACATCCCTTG
Protein sequence predicted based on nucleotide sequence
MFRVGFLIISSSSSLSPLLLVSVVRVNTTNCHKCLLSGTYIFAVLLVCVVFHSGAQEKNY TIREEIPENVLIGNLLKDLNLSLIPNKSLTTTMQFKLVYKTGDVPLIRIEEDTGEIFTTG ARIDREKLCAGIPRDEHCFYEVEVAILPDEIFRLVKIRFLIEDINDNAPLFPATVINISI PENSAINSKYTLPAAVDPDVGINGVQNYELIKSQNIFGLDVIETPEGDKMPQLIVQKELD REEKDTYVMKVKVEDGGFPQRSSTAILQVSVTDTNDNHPVFKETEIEVSIPENAPVGTSV TQLHATDADIGENAKIHFSFSNLVSNIARRLFHLNATTGLITIKEPLDREETPNHKLLVL ASDGGLMPARAMVLVNVTDVNDNVPSIDIRYIVNPVNDTVVLSENIPLNTKIALITVTDK DADHNGRVTCFTDHEIPFRLRPVFSNQFLLENAAYLDYESTKEYAIKLLAADAGKPPLNQ SAMLFIKVKDENDNAPVFTQSFVTVSIPENNSPGIQLMKVSATDADSGPNAEINYLLGPD APPEFSLDRRTGMLTVVKKLDREKEDKYLFTILAKDNGVPPLTSNVTVFVSIIDQNDNSP VFTHNEYKFYVPENLPRHGTVGLITVTDPDYGDNSAVTLSILDENDDFTIDSQTGVIRPN ISFDREKQESYTFYVKAEDGGRVSRSSSAKVTINVVDVNDNKPVFIVPPYNYSYELVLPS TNPGTVVFQVIAVDNDTGMNAEVRYSIVGGNTRDLFAIDQETGNITLMEKCDVTDLGLHR VLVKANDLGQPDSLFSVVIVNLFVNESVTNATLINELVRKSIEAPVTPNTEIADVSSPTS DYVKILVAAVAGTITVVVVIFITAVVRCRQAPHLKAAQKNMQNSEWATPNPENRQMIMMK KKKKKKKHSPKNLLLNVVTIEETKADDVDSDGNRVTLDLPIDLEEQTMGKYNWVTTPTTF KPDSPDLARHYKSASPQPAFQIQPETPLNLKHHIIQELPLDNTFVACDSISNCSSSSSDP YSVSDCGYPVTTFEVPVSVHTRPSQRRVTFHLPEGSQESSSDGGLGDHDAGSLTSTSHGL PLGYPQEEYFDRATPSNRTEGDGNSDPESTFIPGLKKEITVQPTVEEASDNCTQECLIYG HSDACWMPASLDHSSSSQAQASALCHSPPLSQASTQHHSPPVTQTIVLCHSPPVTQTIAL CHSPPPIQVSALHHSPPLVQGTALHHSPPSAQASALCYSPPLAQAAAISHSSSLPQVIAL HRSQAQSSVSLQQGWVQGANGLCSVDQGVQGSATSQFYTMSERLHPSDDSIKVIPLTTFA PRQQARPSRGDSPIMETHPL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4757
Start on reference sequence
735
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
