Gene:
Gene Symbol:
SEC31B
Gene Name:
SEC31 homolog B (S. cerevisiae)
Original Clone ID:
FLH270062.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00083089
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGCTGAAGGAACTTGAGCGGCCAGCTGTCCAGGCATGGAGCCCAGCCAGCCAATAC
CCTTTGTATCTGGCCACAGGAACATCTGCCCAACAGCTAGATTCCTCCTTCAGCACAAAT
GGCACATTGGAAATATTTGAGGTTGATTTCAGGGACCCTTCTCTGGACTTGAAACACAGA
GGAGTCCTTTCTGCCTTGAGCAGGTTTCACAAGCTGGTCTGGGGGAGCTTTGGCAGTGGG
CTTCTGGAAAGCTCCGGGGTTATTGTTGGCGGCGGGGACAATGGCATGCTTATTCTATAC
AATGTGACCCACATCCTGTCTTCGGGGAAGGAGCCTGTGATTGCTCAGAAACAGAAGCAC
ACGGGGGCTGTCAGAGCCCTCGACTTGAATCCTTTCCAGGGCAACCTCCTGGCTTCAGGG
GCCAGCGATTCTGAAATCTTCATTTGGGATCTGAATAACTTGAATGTGCCAATGACCCTG
GGATCCAAGTCACAGCAGCCTCCAGAGGACATCAAGGCACTGTCTTGGAACCGGCAAGCC
CAACACATTCTGTCTTCTGCTCACCCCAGTGGCAAGGCAGTTGTGTGGGATCTCAGGAAG
AATGAACCTATCATCAAAGTCAGTGATCACAGCAACAGGATGCACTGCTCAGGCCTGGCC
TGGCATCCTGACATAGCCACCCAGTTAGTGCTGTGCTCAGAGGATGATCGACTTCCCGTG
ATTCAGCTGTGGGACTTGCGCTTTGCCTCCTCGCCCTTGAAGGTGCTGGAGAGCCACAGC
AGGGGGATCTTGTCAGTGTCATGGAGCCAGGCTGATGCTGAGCTGCTGCTCACTAGTGCT
AAGGACAGCCAGATCTTGTGCCGGAACCTGGGGAGCAGTGAGGTGGTATATAAGCTACCA
ACACAGAGCAGCTGGTGCTTTGATGTGCAGTGGTGCCCTCGGGACCCTTCAGTGTTCTCT
GCTGCCTCCTTCAACGGCTGGATCAGTTTGTACTCTGTGATGGGTAGGAGCTGGGAAGTC
CAGCATATGAGACAGGCTGACAAGATCTCCTCTTCCTTCAGCAAAGGCCAGCCTCTCCCA
CCACTGCAGGTGCCAGAGCAAGTGGCACAAGCACCACTGATACCTCCCCTGAAAAAACCC
CCCAAATGGATTAGAAGACCAACAGGTGTTTCATTTGCTTTTGGAGGGAAGCTGGTTACT
TTTGGCCTCCCCAGCACCCCTGCCCATCTGGTGCCACAGCCTTGCCCCCGCCTAGTCTTC
ATCAGTCAAGTCACCACAGAATCTGAATTCCTGATGCGATCAGCTGAGCTGCAGGAGGCC
TTGGGATCAGGAAATCTACTGAATTACTGTCAGAACAAGAGCCAGCAAGCTTTACTGCAA
AGTGAAAAGATGCTGTGGCAGTTCCTGAAGGTGACCTTAGAGCAAGACTCCAGAATGAAA
TTCCTAAAGCTTTTAGGATACAGTAAAGATGAGCTTCAGAAGAAGGTGGCCACATGGTTG
AAGAGTGACGTGGGGCTAGGTGAGAGTCCTCAGCCCAAGGGAAATGACCTCAACAGTGAC
AGACAACAGGCCTTCTGCAGCCAGGCCTCCAAACACACCACAAAGGAAGCCTCTGCTTCC
TCAGCCTTCTTTGATGAGCTGGTCCCTCAGAACATGACTCCTTGGGAGATCCCCATCACA
AAAGATATTGATGGACTCCTAAGCCAGGCTCTCCTGCTTGGGGAACTGGGTCCGGCCGTG
GAGCTGTGTCTGAAGGAGGAGCGCTTTGCTGATGCCATTATCCTGGCCCAGGCTGGGGGT
ACAGATCTGCTGAAGCAAACACAGGAGCGCTACTTGGCCAAGAAGAAAACCAAAATCTCC
TCGCTTCTAGCCTGTGTTGTGCAAAAGAATTGGAAGGATGTGGTGTGTACCTGTAGCCTG
AAGAACTGGAGAGAGGCACTGGCTTTGCTACTGACATACTCAGGCACAGAGAAATTTCCC
GAGCTCTGTGACATGCTGGGAACTCGCATGGAACAGGAGGGCAGCAGGGCACTAACCTCC
GAAGCCAGACTCTGTTATGTGTGCTCAGGGAGTGTGGAGCGGCTGGTGGAGTGCTGGGCA
AAATGCCACCAGGCTTTGTCCCCCATGGCTCTGCAGGACCTGATGGAGAAGGTGATGGTT
CTTAACAGGAGCTTGGAGCAACTGCGGGGTCCTCATGGGGTGAGCCCAGGCCCTGCCACA
ACCTACAGGGTCACTCAGTATGCCAACCTCCTGGCAGCCCAGGGCAGCCTGGCCACTGCC
ATGAGCTTTCTACCCAGGGACTGTGCTCAGCCACCAGTTCAGCAGCTAAGAGATCGGCTT
TTTCATGCTCAAGGTTCTGCTGTCTTGGGCCAACAGTCTCCCCCTTTCCCCTTCCCCCGG
ATTGTTGTGGGAGCTACCCTCCACTCTAAAGAGACATCATCTTACAGATTGGGATCCCAG
CCTTCTCACCAGGTCCCAACTCCATCTCCAAGGCCAAGGGTTTTCACCCCTCAGTCATCA
CCAGCGATGCCCTTGGCACCTTCCCATCCTAGCCCTTATCAGGGTCCCAGGACACAGAAT
ATAAGTGACTACAGGGCACCTGGGCCCCAGGCCATCCAGCCTTTGCCTTTGAGCCCTGGG
GTAAGGCCTGCTTCATCTCAGCCACAGCTATTAGGAGGGCAAAGGGTGCAAGTTCCTAAC
CCGGTGGGATTCCCTGGGACATGGCCTCTTCCTGGTTCCCCTCTACCCATGGCATGCCCA
GGCATCATGCGACCTGGCTCTACCTCCCTGCCTGAGACTCCTAGACTGTTCCCTCTGCTT
CCTCTGAGACCACTAGGTCCCGGCCGCATGGTCTCCCACACCCCAGCCCCTCCTGCAAGC
TTCCCTGTGCCATACCTTCCAGGGGACCCAGGTGCCCCATGCTCTAGTGTCCTCCCAACC
ACTGGCATCTTGACTCCTCACCCAGGACCTCAAGATTCCTGGAAAGAAGCCCCAGCCCCC
AGGGGAAACCTCCAGAGGAACAAGCTGCCAGAGACATTTATGCCCCCAGCACCAATTACT
GCTCCAGTTATGAGCCTCACCCCTGAGCTACAAGGGATTCTTCCCTCACAGCCCCCTGTC
TCCAGTGTGAGTCATGCTCCCCCAGGAGTTCCAGGAGAACTCAGCCTGCAGCTTCAGCAC
CTGCCACCTGAGAAGATGGAAAGGAAGGAGCTGCCCCCAGAGCATCAGTCCTTGAAGAGC
AGCTTTGAGGCGCTTCTCCAACGCTGCTCCCTGTCTGCAACTGACTTAAAGACAAAAAGG
AAGCTGGAAGAGGCAGCCCAGCGTCTGGAGTATCTATATGAGAAGCTCTGTGAGGGGACA
CTCTCACCTCATGTCGTGGCTGGGCTCCATGAGGTTGCCCGATGTGTGGATGCAGGAAGC
TTTGAGCAGGGCCTTGCAGTGCATGCCCAGGTGGCGGGCTGTAGCAGCTTCAGCGAGGTG
TCCAGCTTCATGCCTATCCTGAAGGCTGTCCTCATCATCGCTCATAAGCTGCTGGTC
Protein sequence predicted based on nucleotide sequence
MKLKELERPAVQAWSPASQYPLYLATGTSAQQLDSSFSTNGTLEIFEVDFRDPSLDLKHR GVLSALSRFHKLVWGSFGSGLLESSGVIVGGGDNGMLILYNVTHILSSGKEPVIAQKQKH TGAVRALDLNPFQGNLLASGASDSEIFIWDLNNLNVPMTLGSKSQQPPEDIKALSWNRQA QHILSSAHPSGKAVVWDLRKNEPIIKVSDHSNRMHCSGLAWHPDIATQLVLCSEDDRLPV IQLWDLRFASSPLKVLESHSRGILSVSWSQADAELLLTSAKDSQILCRNLGSSEVVYKLP TQSSWCFDVQWCPRDPSVFSAASFNGWISLYSVMGRSWEVQHMRQADKISSSFSKGQPLP PLQVPEQVAQAPLIPPLKKPPKWIRRPTGVSFAFGGKLVTFGLPSTPAHLVPQPCPRLVF ISQVTTESEFLMRSAELQEALGSGNLLNYCQNKSQQALLQSEKMLWQFLKVTLEQDSRMK FLKLLGYSKDELQKKVATWLKSDVGLGESPQPKGNDLNSDRQQAFCSQASKHTTKEASAS SAFFDELVPQNMTPWEIPITKDIDGLLSQALLLGELGPAVELCLKEERFADAIILAQAGG TDLLKQTQERYLAKKKTKISSLLACVVQKNWKDVVCTCSLKNWREALALLLTYSGTEKFP ELCDMLGTRMEQEGSRALTSEARLCYVCSGSVERLVECWAKCHQALSPMALQDLMEKVMV LNRSLEQLRGPHGVSPGPATTYRVTQYANLLAAQGSLATAMSFLPRDCAQPPVQQLRDRL FHAQGSAVLGQQSPPFPFPRIVVGATLHSKETSSYRLGSQPSHQVPTPSPRPRVFTPQSS PAMPLAPSHPSPYQGPRTQNISDYRAPGPQAIQPLPLSPGVRPASSQPQLLGGQRVQVPN PVGFPGTWPLPGSPLPMACPGIMRPGSTSLPETPRLFPLLPLRPLGPGRMVSHTPAPPAS FPVPYLPGDPGAPCSSVLPTTGILTPHPGPQDSWKEAPAPRGNLQRNKLPETFMPPAPIT APVMSLTPELQGILPSQPPVSSVSHAPPGVPGELSLQLQHLPPEKMERKELPPEHQSLKS SFEALLQRCSLSATDLKTKRKLEEAAQRLEYLYEKLCEGTLSPHVVAGLHEVARCVDAGS FEQGLAVHAQVAGCSSFSEVSSFMPILKAVLIIAHKLLV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
103
End on reference sequence
3642
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
