Gene:
Gene Symbol:
PLXNC1
Gene Name:
plexin C1
Original Clone ID:
FLH270068.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00083095
Price:
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UNAVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGGTCTCCCGGAGGAAGGCGCCGCCGCGCCCCCCGCGCCCCGCAGCGCCACTGCCC
CTGCTCGCCTATCTGCTGGCACTGGCGGCTCCCGGCCGGGGCGCGGACGAGCCCGTGTGG
CGGTCGGAGCAAGCCATCGGAGCCATCGCGGCGAGCCAGGAGGACGGCGTGTTTGTGGCG
AGCGGCAGCTGCCTGGACCAGCTGGACTACAGCCTGGAGCACAGCCTCTCGCGCCTGTAC
CGGGACCAAGCGGGCAACTGCACAGAGCCGGTCTCGCTGGCGCCCCCCGCGCGGCCCCGG
CCCGGGAGCAGCTTCAGCAAGCTGCTGCTGCCCTACCGCGAGGGGGCGGCCGGCCTCGGG
GGGCTGCTGCTCACCGGCTGGACCTTCGACCGGGGCGCCTGCGAGGTGCGGCCCCTGGGC
AACCTGAGCCGCAACTCCCTGCGCAACGGCACCGAGGTGGTGTCGTGCCACCCGCAGGGC
TCGACGGCCGGCGTGGTGTACCGCGCGGGCCGGAACAACCGCTGGTACCTGGCGGTGGCC
GCCACCTACGTGCTGCCTGAGCCGGAGACGGCGAGCCGCTGCAACCCCGCGGCATCCGAC
CACGACACGGCCATCGCGCTCAAGGACACGGAGGGGCGCAGCCTGGCCACGCAGGAGCTG
GGGCGCCTCAAGCTGTGCGAGGGCGCGGGCAGCCTGCACTTCGTGGACGCCTTTCTCTGG
AACGGCAGCATCTACTTCCCCTACTACCCCTACAACTATACGAGCGGCGCTGCCACCGGC
TGGCCCAGCATGGCGCGCATCGCGCAGAGCACCGAGGTGCTGTTCCAGGGCCAGGCATCC
CTCGACTGCGGCCACGGCCACCCCGACGGCCGCCGCCTGCTCCTCTCCTCCAGCCTAGTG
GAGGCCCTGGACGTCTGGGCGGGAGTGTTCAGCGCGGCCGCTGGAGAGGGCCAGGAGCGG
CGCTCCCCCACCACCACGGCGCTCTGCCTCTTCAGAATGAGTGAGATCCAGGCGCGCGCC
AAGAGGGTCAGCTGGGACTTCAAGACGGCCGAGAGCCACTGCAAAGAAGGGGATCAACCT
GAAAGAGTCCAACCAATCGCATCATCTACCTTGATCCATTCCGACCTGACATCCGTTTAT
GGCACCGTGGTAATGAACAGGACTGTTTTATTCTTGGGGACTGGAGATGGCCAGTTACTT
AAGGTTATTCTTGGTGAGAATTTGACTTCAAATTGTCCAGAGGTTATCTATGAAATTAAA
GAAGAGACACCTGTTTTCTACAAACTCGTTCCTGATCCTGTGAAGAATATCTACATTTAT
CTAACAGCTGGGAAAGAGGTGAGGAGAATTCGTGTTGCAAACTGCAATAAACATAAATCC
TGTTCGGAGTGTTTAACAGCCACAGACCCTCACTGCGGTTGGTGCCATTCGCTACAAAGG
TGCACTTTTCAAGGAGATTGTGTACATTCAGAGAACTTAGAAAACTGGCTGGATATTTCG
TCTGGAGCAAAAAAGTGCCCTAAAATTCAGATAATTCGAAGCAGTAAAGAAAAGACTACA
GTGACTATGGTGGGAAGCTTCTCTCCAAGACACTCAAAGTGCATGGTGAAGAATGTGGAC
TCTAGCAGGGAGCTCTGCCAGAATAAAAGTCAGCCCAACCGGACCTGCACCTGTAGCATC
CCAACCAGAGCAACCTACAAAGATGTTTCAGTTGTCAACGTGATGTTCTCCTTCGGTTCT
TGGAATTTATCAGACAGATTCAACTTTACCAACTGCTCATCATTAAAAGAATGCCCAGCA
TGCGTAGAAACTGGCTGCGCGTGGTGTAAAAGTGCAAGAAGGTGTATCCACCCCTTCACA
GCTTGCGACCCTTCTGATTATGAGAGAAACCAGGAACAGTGTCCAGTGGCTGTCGAGAAG
ACATCAGGAGGAGGAAGACCCAAGGAGAACAAGGGGAACAGAACCAACCAGGCTTTACAG
GTCTTCTACATTAAGTCCATTGAGCCACAGAAAGTATCGACATTAGGGAAAAGCAACGTG
ATAGTAACGGGAGCAAACTTTACCCGGGCATCGAACATCACAATGATCCTGAAAGGAACC
AGTACCTGTGATAAGGATGTGATACAGGTTAGCCATGTGCTAAATGACACCCACATGAAA
TTCTCTCTTCCATCAAGCCGGAAAGAAATGAAGGATGTGTGTATCCAGTTTGATGGTGGG
AACTGCTCTTCTGTGGGATCCTTATCCTACATTGCTCTGCCACATTGTTCCCTTATATTT
CCTGCTACCACCTGGATCAGTGGTGGTCAAAATATAACCATGATGGGCAGAAATTTTGAT
GTAATTGACAACTTAATCATTTCACATGAATTAAAAGGAAACATAAATGTCTCTGAATAT
TGTGTGGCGACTTACTGCGGGTTTTTAGCCCCCAGTTTAAAGAGTTCAAAAGTGCGCACG
AATGTCACTGTGAAGCTGAGAGTACAAGACACCTACTTGGATTGTGGAACCCTGCAGTAT
CGGGAGGACCCCAGATTCACGGGGTATCGGGTGGAATCCGAGGTGGACACAGAACTGGAA
GTGAAAATTCAAAAAGAAAATGACAACTTCAATATTTCCAAAAAAGACATTGAAATTACT
CTCTTCCATGGGGAAAATGGGCAATTAAATTGCAGTTTTGAAAATATTACTAGAAATCAA
GATCTTACCACCATCCTTTGCAAAATTAAAGGCATCAAGACTGCAAGCACCATTGCCAAC
TCTTCTAAGAAAGTTCGGGTCAAGCTGGGAAACCTGGAGCTCTACGTCGAGCAGGAGTCA
GTTCCTTCCACATGGTATTTTCTGATTGTGCTCCCTGTCTTGCTAGTGATTGTCATTTTT
GCGGCCGTGGGGGTGACCAGGCACAAATCGAAGGAGCTGAGTCGCAAACAGAGTCAACAA
CTAGAATTGCTGGAAAGCGAGCTCCGGAAAGAGATACGTGACGGCTTTGCTGAGCTGCAG
ATGGATAAATTGGATGTGGTTGATAGTTTTGGAACTGTTCCCTTCCTTGACTACAAACAT
TTTGCTCTGAGAACTTTCTTCCCTGAGTCAGGTGGCTTCACCCACATCTTCACTGAAGAT
ATGCATAACAGAGACGCCAACGACAAGAATGAAAGTCTCACAGCTTTGGATGCCCTAATC
TGTAATAAAAGCTTTCTTGTTACTGTCATCCACACCCTTGAAAAGCAGAAGAACTTTTCT
GTGAAGGACAGGTGTCTGTTTGCCTCCTTCCTAACCATTGCACTGCAAACCAAGCTGGTC
TACCTGACCAGCATCCTAGAGGTGCTGACCAGGGACTTGATGGAACAGTGTAGTAACATG
CAGCCGAAACTCATGCTGAGACGCACGGAGTCCGTCGTCGAAAAACTCCTCACAAACTGG
ATGTCCGTCTGCCTTTCTGGATTTCTCCGGGAGACTGTCGGAGAGCCCTTCTATTTGCTG
GTGACGACTCTGAACCAGAAAATTAACAAGGGTCCCGTGGATGTAATCACTTGCAAAGCC
CTGTACACACTTAATGAAGACTGGCTGTTGTGGCAGGTTCCGGAATTCAGTACTGTGGCA
TTAAACGTCGTCTTTGAAAAAATCCCGGAAAACGAGAGTGCAGATGTCTGTCGGAATATT
TCAGTCAATGTTCTCGACTGTGACACCATTGGCCAAGCCAAAGAAAAGATTTTCCAAGCA
TTCTTAAGCAAAAATGGCTCTCCTTATGGACTTCAGCTTAATGAAATTGGTCTTGAGCTT
CAAATGGGCACACGACAGAAAGAACTTCTGGACATCGACAGTTCCTCCGTGATTCTTGAA
GATGGAATCACCAAGCTAAACACCATTGGCCACTATGAGATATCAAATGGATCCACTATA
AAAGTCTTTAAGAAGATAGCAAATTTTACTTCAGATGTGGAGTACTCGGATGACCACTGC
CATTTGATTTTACCAGATTCGGAAGCATTCCAAGATGTGCAAGGAAAGAGACATCGAGGG
AAGCACAAGTTCAAAGTAAAAGAAATGTATCTGACAAAGCTGCTGTCGACCAAGGTGGCA
ATTCATTCTGTGCTTGAAAAACTTTTTAGAAGCATTTGGAGTTTACCCAACAGCAGAGCT
CCATTTGCTATAAAATACTTTTTTGACTTTTTGGACGCCCAGGCTGAAAACAAAAAAATC
ACAGATCCTGACGTCGTACATATTTGGAAAACAAACAGCCTTCCTCTTCGCTTCTGGGTA
AACATCCTGAAGAACCCTCAGTTTGTCTTTGACATTAAGAAGACACCACATATAGACGGC
TGTTTGTCAGTGATTGCCCAGGCATTCATGGATGCATTTTCTCTCACAGAGCAGCAACTA
GGGAAGGAAGCACCAACTAATAAGCTTCTCTATGCCAAGGATATCCCAACCTACAAAGAA
GAAGTAAAATCTTATTACAAAGCAATCAGGGATTTGCCTCCATTGTCATCCTCAGAAATG
GAAGAATTTTTAACTCAGGAATCTAAGAAACATGAAAATGAATTTAATGAAGAAGTGGCC
TTGACAGAAATTTACAAATACATCGTAAAATATTTTGATGAGATTCTAAATAAACTAGAA
AGAGAACGAGGGCTGGAAGAAGCTCAGAAACAACTCTTGCATGTAAAAGTCTTATTTGAT
GAAAAGAAGAAATGCAAGTGGATG
Protein sequence predicted based on nucleotide sequence
MEVSRRKAPPRPPRPAAPLPLLAYLLALAAPGRGADEPVWRSEQAIGAIAASQEDGVFVA SGSCLDQLDYSLEHSLSRLYRDQAGNCTEPVSLAPPARPRPGSSFSKLLLPYREGAAGLG GLLLTGWTFDRGACEVRPLGNLSRNSLRNGTEVVSCHPQGSTAGVVYRAGRNNRWYLAVA ATYVLPEPETASRCNPAASDHDTAIALKDTEGRSLATQELGRLKLCEGAGSLHFVDAFLW NGSIYFPYYPYNYTSGAATGWPSMARIAQSTEVLFQGQASLDCGHGHPDGRRLLLSSSLV EALDVWAGVFSAAAGEGQERRSPTTTALCLFRMSEIQARAKRVSWDFKTAESHCKEGDQP ERVQPIASSTLIHSDLTSVYGTVVMNRTVLFLGTGDGQLLKVILGENLTSNCPEVIYEIK EETPVFYKLVPDPVKNIYIYLTAGKEVRRIRVANCNKHKSCSECLTATDPHCGWCHSLQR CTFQGDCVHSENLENWLDISSGAKKCPKIQIIRSSKEKTTVTMVGSFSPRHSKCMVKNVD SSRELCQNKSQPNRTCTCSIPTRATYKDVSVVNVMFSFGSWNLSDRFNFTNCSSLKECPA CVETGCAWCKSARRCIHPFTACDPSDYERNQEQCPVAVEKTSGGGRPKENKGNRTNQALQ VFYIKSIEPQKVSTLGKSNVIVTGANFTRASNITMILKGTSTCDKDVIQVSHVLNDTHMK FSLPSSRKEMKDVCIQFDGGNCSSVGSLSYIALPHCSLIFPATTWISGGQNITMMGRNFD VIDNLIISHELKGNINVSEYCVATYCGFLAPSLKSSKVRTNVTVKLRVQDTYLDCGTLQY REDPRFTGYRVESEVDTELEVKIQKENDNFNISKKDIEITLFHGENGQLNCSFENITRNQ DLTTILCKIKGIKTASTIANSSKKVRVKLGNLELYVEQESVPSTWYFLIVLPVLLVIVIF AAVGVTRHKSKELSRKQSQQLELLESELRKEIRDGFAELQMDKLDVVDSFGTVPFLDYKH FALRTFFPESGGFTHIFTEDMHNRDANDKNESLTALDALICNKSFLVTVIHTLEKQKNFS VKDRCLFASFLTIALQTKLVYLTSILEVLTRDLMEQCSNMQPKLMLRRTESVVEKLLTNW MSVCLSGFLRETVGEPFYLLVTTLNQKINKGPVDVITCKALYTLNEDWLLWQVPEFSTVA LNVVFEKIPENESADVCRNISVNVLDCDTIGQAKEKIFQAFLSKNGSPYGLQLNEIGLEL QMGTRQKELLDIDSSSVILEDGITKLNTIGHYEISNGSTIKVFKKIANFTSDVEYSDDHC HLILPDSEAFQDVQGKRHRGKHKFKVKEMYLTKLLSTKVAIHSVLEKLFRSIWSLPNSRA PFAIKYFFDFLDAQAENKKITDPDVVHIWKTNSLPLRFWVNILKNPQFVFDIKKTPHIDG CLSVIAQAFMDAFSLTEQQLGKEAPTNKLLYAKDIPTYKEEVKSYYKAIRDLPPLSSSEM EEFLTQESKKHENEFNEEVALTEIYKYIVKYFDEILNKLERERGLEEAQKQLLHVKVLFD EKKKCKWM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
250
End on reference sequence
4956
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
