Gene:
Gene Symbol:
Dsg1c
Gene Name:
desmoglein 1 gamma
Original Clone ID:
FLH270198.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083127
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2736nts
Open reading frame: 1 to 2736
ATGGACTGGCACTCCTTCAGGATAGCTGCCCTGCTGCTCACTTCCCTGGTGGTGCTTGAA
GTTAACAGTGAATTTCAAATCCAGGTAAGAGATCATAACGCCAAAAATGGTACCATCAAG
TGGCATTCCATCAGAAGGCAGAAACGAGAATGGATCAAGTTTGCTGCAGCCTGTCGCGAA
GGTGAAGACAACTCAAAGAGAAACCCAATTGCCAAAATTCATTCAGACTGTGCTGCAAAC
CAACCAGTGACATACCGCATCTCGGGAGTGGGAATTGACCAGCCACCTTATGGAATCTTC
ATTATTAACCAAAAAACTGGTGAAATCAATATAACATCCATTGTTGATCGTGAAGTCACC
CCCTTTTTCATTATCTATTGCCGAGCACTGAATGCACAAGGTCAAGACTTGGAGAATCCA
CTGGAGCTTAGAGTTAGAGTGATGGATATAAACGACAACCCTCCTGTGTTTTCAATGACT
ACATTTCTAGGACAAATAGAAGAAAATTCTAATGCAAACACACTGGTAATGAAACTCAAT
GCTACCGATGCCGATGAGCCAAATAACTTGAACTCAATGATAGCCTTCAAGATCATAAGA
CAGGAGCCTTCTGACTCACCAATGTTCACCATCAACAGGAAAACTGGAGAAATCAGAACA
ATGAATAACTTTCTAGACAGAGAGCAATATAGCCAGTATTCCCTTGTGGTGAGAGGCTCA
GACCGGGACGGTGGAGCAGACGGCATGTCTGCAGAGAGCGAGTGCAGCATCACCATCCTG
GATGTCAATGACAACATCCCATACTTGGAGCAGTCATCTTATGACATCACAATTGAAGAA
AACACACTTCACTCCCAACTATTGCAGATCAGAGTCATTGATTTGGATGAAGAATTCTCA
GACAACTGGAAGGCAATCATTTTCTTTATCTCTGGAAATGAGGGAAATTGGTTTGAAATA
GAAATGAATGAAAGAACAAATGTAGGAACTCTGAAGGTTGTCAAGCCCCTAGATTATGAA
GCCGTGAAGAATCTACAGCTTTGTATTGGTGTTCGAAATGTAGCTGAATTCCACCAGTCA
ATTATTTCTCAATATAGACTCACAGTAACTTTGATCACTGTGACTGTGTTAAATGTTGTC
AAGGGCTGTGTGTTCCAGCCAGGTTCAAAGACATTTATAGTAGACAGTAGAATGGAAGCA
AATCACACAGTGGGAGAGTTTTTAGCTACAGACTGTGAAACAGGTCAAGCTACAAACAAG
TTCAAAAATGTTAGATACAGATATGTAATGGGAAATAACCCAGAGAACCTGCTGGTTGTT
GACTCAGGCATAATAACTTTAAGAAACAGAGTTACTATGGAACAATACGAAAGGCTTAAT
AAGAGATATGAAGGAACAGTGCTATCAATACATGATTCCCTTCAGAGGACCTGCACAGGG
ACAATCATCATGGTACTTTGTGGCTTCTGGACAACTACTGAACATCCCACAACATCCACT
GAGAAACCTGTGACTCTATCCATCACTCCAAACGTAGACAATGTTCACTTCGGTCCTGCA
GGCATCGGGCTGCTCATCATGGGGTTCCTAGTCTTAGGATTGGTTCCATTCCTGCTGATC
TCCTGTGATTGTGGAGGGGCCCCTGGTGGTGGAGCTGGATTTGAACCTGTTCCAGAATGT
TCTGATGGAGCAATTCACACGTGGGCTGTAGAAGGGCCACAGCCTGGTGGGATAACCACC
ATCTGTGTGCCACAAATGCCACCTGGTAATGCCAATGTTATAGAATATATTGACAACTCA
GGAGTTTATACAAATGAATATTGTGGCAGAGAAATGCAAGATCTGGGAGGAGGAGAACGA
ACCACAGGATTTGAACTAATGGATGGAGTTAAAACATCAGCTGCACCTGAGATATGCCAA
GAATATTCAGGAACATTAAGAAGAAATTCAATGAGAGAATGTAGAGATGGGGGTCTCAAC
ATGAATTTTATGGAAAGTTACTTCTGCCAGAAAGCCTATGCTTATGCGGATGAAGATGAA
GGACGTCCATCCAATGACTGTTTGCTCATCTATGACATCGAAGGTGTGGGTTCCCCTGCA
GGCTCCGTGGGCTGTTGCAGTTTCATTGAAGATCTAGATGAGAGTTTCCTGGATACCTTG
GGGCCCAAGTTTAAGAAGTTGGCAGATATCAGCCTGGGAAAAGAAATAGACTCATACCCA
GATCCTGATCCTTCTTGGCCTCCTCAGAGCACTGAACCAATTTGCCCACAGCATATGGAG
CAACTTGCTAGTGGACACCCAAGCATATCCCCACATTTTGGCAAGACCACAGTAATTTCT
GAGAATGCCTACCCCTCTGGCCCTGGAGTACAGCATCCTATGCTAATTCCTGATCCTCTG
GGCTATGGTAATATCACTGTGAGGGAGTCCTATACTACCTCTGGCACTCTGAAACCCTCT
GTCCACTTTCATGATAACCAGCAAGCTTCAAATGTGGTGGTGACAGAGAGAGTGGTGGGC
CCCATCTCTGGTGCTGATTTGCATGGCATGTTAGAGATACCTGCCTTAAGAGATGGAACA
AATGTTATAGTGACAGAAAGGGTAATAGCACCAGGCTCAAGTTTACCCAATTCTCTAACC
ATTCCTAACCCTCGAGAGACCTCAAATGTAGTTGTGACAGAAAGAGTGATCCAACCAACC
TCTGGCATGATAGGCAACCTGAGCATCCCCCCCTAA
Protein sequence predicted based on nucleotide sequence
MDWHSFRIAALLLTSLVVLEVNSEFQIQVRDHNAKNGTIKWHSIRRQKREWIKFAAACRE GEDNSKRNPIAKIHSDCAANQPVTYRISGVGIDQPPYGIFIINQKTGEINITSIVDREVT PFFIIYCRALNAQGQDLENPLELRVRVMDINDNPPVFSMTTFLGQIEENSNANTLVMKLN ATDADEPNNLNSMIAFKIIRQEPSDSPMFTINRKTGEIRTMNNFLDREQYSQYSLVVRGS DRDGGADGMSAESECSITILDVNDNIPYLEQSSYDITIEENTLHSQLLQIRVIDLDEEFS DNWKAIIFFISGNEGNWFEIEMNERTNVGTLKVVKPLDYEAVKNLQLCIGVRNVAEFHQS IISQYRLTVTLITVTVLNVVKGCVFQPGSKTFIVDSRMEANHTVGEFLATDCETGQATNK FKNVRYRYVMGNNPENLLVVDSGIITLRNRVTMEQYERLNKRYEGTVLSIHDSLQRTCTG TIIMVLCGFWTTTEHPTTSTEKPVTLSITPNVDNVHFGPAGIGLLIMGFLVLGLVPFLLI SCDCGGAPGGGAGFEPVPECSDGAIHTWAVEGPQPGGITTICVPQMPPGNANVIEYIDNS GVYTNEYCGREMQDLGGGERTTGFELMDGVKTSAAPEICQEYSGTLRRNSMRECRDGGLN MNFMESYFCQKAYAYADEDEGRPSNDCLLIYDIEGVGSPAGSVGCCSFIEDLDESFLDTL GPKFKKLADISLGKEIDSYPDPDPSWPPQSTEPICPQHMEQLASGHPSISPHFGKTTVIS ENAYPSGPGVQHPMLIPDPLGYGNITVRESYTTSGTLKPSVHFHDNQQASNVVVTERVVG PISGADLHGMLEIPALRDGTNVIVTERVIAPGSSLPNSLTIPNPRETSNVVVTERVIQPT SGMIGNLSIPP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
101
End on reference sequence
2836
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
