Srgap2 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Srgap2
Gene Name:  SLIT-ROBO Rho GTPase activating protein 2
Original Clone ID:
FLH270217.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083145
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3294nts
Open reading frame: 1 to 3294

ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATCGCAGAGTACGATACTCAG
GTCAAAGAGATTAGGGCTCAGCTCACAGAGCAGATGAAATGCCTAGATCAGCAATGTGAG
CTCCGGGTGCAGCTGTTGCAGGACCTGCAGGACTTCTTCCGCAAGAAGGCTGAGATTGAA
ATGGACTACTCTCGAAACCTGGAGAAGCTAGCCGAGCGCTTTCTAGCCAAGACACGAAGC
ACCAAGGACCAGCAATTTAAGAAGGACCAGAATGTTCTTTCTCCAGTCAACTGCTGGAAC
CTCCTCTTAAACCAAGTGAAGCGGGAGAGCAGGGATCATACCACCCTGAGTGACATCTAC
CTGAATAATATAATTCCTCGATTTGTTCAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA
AAGAGTAAAGAAGTTGGCCAGCAACTCCAAGATGATTTGATGAAAGTCCTGAACGAGCTT
TACTCGGTCATGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAA
CTGAAGGAAGCAGAGAAACAAGAAGAGAAGCAAATTGGCAAATCAGTAAAGCAAGAGGAC
CGGCAGACCCCTCGCTCCCCTGACTCCACAGCCAATGTCCGCATTGAGGAGAAGCATGTC
CGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGAAGCGACAAGCCAAGTACACA
GAAAATAAGCTGAAGGCCATTAAAGCCCGGAATGAGTATTTATTGGCTTTGGAGGCAACC
AATGCATCTGTCTTCAAGTACTATATCCATGACCTGTCTGATATTATTGATCAATGTTGT
GACCTAGGCTACCATGCTAGCCTGAACCGGGCTCTACGCACTTTCCTATCTGCTGAATTA
AATCTGGAACAGTCAAAACATGAAGGTCTGGATGCTATTGAAAATGCAGTAGAAAACCTA
GATGCCACCAGTGACAAGCAACGGCTCATGGAGATGTACAACAATGTTTTTTGCCCCCCT
ATGAAATTTGAATTCCAGCCCCACATGGGAGATATGGCCTCTCAACTCTGTGCCCAGCAG
CCTGTCCAGAGTGAGCTGGTGCAGAGATGCCAACAGCTGCAGTCTCGCTTATCCACTTTG
AAGATTGAGAATGAAGAGGTGAAAAAGACAATGGAGGCCACCCTGCAGACCATTCAAGAC
ATTGTGACTGTTGAGGATTTTGATGTATCTGACTGCTTCCAGTATAGCAATTCCATGGAG
TCTGTCAAATCAACTGTCTCTGAAACATTCATGAGCAAGCCCAGCATTGCTAAGAGGAGA
GCGAACCAGCAAGAGACGGAACAGTTTTATTTCACGAAAATGAAGGAGTACTTGGAGGGT
AGGAACCTCATCACAAAGCTACAAGCCAAGCATGACCTCCTGCAGAAAACCCTGGGAGAA
AGTCAGCGGACAGACTGTAGTCTTGCCAGGCCTCCTTGCTCCACTCCAGCCCCTACAAAG
CAAATCCTCTTTTTTCCACTCTTGTTTATCCTCCATAACTTGTATGGTTTTGACCTTGAC
ATACTTTTCAAAGATTCCAGCCAAGCAATTCCTCTGGTGGTAGAAAGCTGCATCCGGTTT
ATTAGCAGACATGGCCTACAACATGAGGGAATTTTCCGGGTTTCTGGATCACAAGTAGAA
GTGAACGACATAAAAAACGCCTTTGAGAGAGGAGAGGATCCCCTGGCTGGGGACCAGAAT
GACCATGACATGGACTCTATAGCTGGTGTCCTCAAGCTTTACTTCCGGGGCCTGGAACAC
CCGCTCTTCCCTAAAGACATCTTCCATGACTTGATTGCCTGTGTCACAATGGACAACCTG
CAAGAGAGAGCTGTGCATATCCGGAAAGTCCTTCTGGTCCTGCCCAAGCCCACTCTGATT
ATCATGAGATATCTCTTTGCCTTCCTCAATCACTTATCACAGTTCAGTGAAGAGAACATG
ATGGACCCCTACAACCTTGCCATCTGCTTCGGGCCCTCACTGATGTCAGTGCCAGAGGGC
CACGACCAGGTGTCCTGTCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAA
CATGAGAACATTTTCCCAAACCCCAGGGAGCTGGAGGGTCCCATCTACAGCAGAGGAGGA
AGCATGGAGGATTACTGTGACAGCACTCATGGAGAGACTACCTCTGCTGAAGACTCCACC
CAGGACGTTACAGCGGAGCACCACACAAGCGATGATGAATGTGAGCCCATAGAAGCCATT
GCCAAGTTTGACTACGTAGGCCGGACAGCCCGAGAACTGTCTTTCAAGAAGGGAGCATCC
CTGCTGCTCTACCAGCGAGCTTCTGATGACTGGTGGGAGGGCCGGCACAACGGTATAGAT
GGACTCATCCCCCATCAGTACATCGTAGTCCAAGACACCGAGGACGGTGTCGTGGAGAGG
TCCAGCCCCAAGTCTGAGATTGAGGTCATGTCTGAGCCACCTGAAGAAAAGGTGACAGCC
AGAACGGGGGCCAGCTGTCCCACTAATCACATTTTTTTCAATATTTACAAGTCTGTGTTA
ATTATGCGTCTACAATCACAAGGAGCGAGTATGCCAGAAAGAAAAGCTGGAGATGACAGC
CATGGGCTGGGCAGTTCTCTGACTGACTCCTCCTCCCTGGGGGTGGGGGCTAGCTGCCGT
CCATCCTCCCAGCCCATCATGAGCCAGAATCTCCCCAAGGAAGGGCCAGATAAGTGTTCC
ATCAGCGGCCATGGCAGCCTCAACTCTATCAGCCGCCACTCATCCTTGAAGAACCGGATG
GACAGTCCGCAGATCCGGAAGACCGCTACGGCAGGAAGGTCAAAAAGTTTCAATAACCAT
CGGCCCATGGACCCTGAAGTCATTGCACAGGATATTGAAGCAACAATGAACTCTGCCCTG
AATGAGCTTCAAGAGCTAGAGCGGCAGAGCAGTGCTAAGCACACACCTGATGTGGTTCTG
GACACCTTGGAACCACTCAAGACCTCCCCGGTGGTAGCCCCCACATCTGAGCCCTCCAGC
CCTCTGCACACCCAGCTCCTCAAGGACCCTGAGCCTGCCTTCCAGCGCAGCGCTAGTACT
GCTGGGGACATTGCCTGCGCCTTCCGGCCTGTAAAGTCTGTCAAGATGGCTGCTCCAGTC
AAACCACCAGCCACACGGCCCAAGCCAACTGTCTTCCCCAAAACAAACGCCACTAGCCCT
GGTGTCAATTCATCTGCTTCCCCACAGGCCACTGACAAGTCTTGTACTGTCTAA

Protein sequence predicted based on nucleotide sequence

MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIE
MDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIY
LNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSK
LKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYT
ENKLKAIKARNEYLLALEATNASVFKYYIHDLSDIIDQCCDLGYHASLNRALRTFLSAEL
NLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQ
PVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME
SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGE
SQRTDCSLARPPCSTPAPTKQILFFPLLFILHNLYGFDLDILFKDSSQAIPLVVESCIRF
ISRHGLQHEGIFRVSGSQVEVNDIKNAFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEH
PLFPKDIFHDLIACVTMDNLQERAVHIRKVLLVLPKPTLIIMRYLFAFLNHLSQFSEENM
MDPYNLAICFGPSLMSVPEGHDQVSCQAHVNELIKTIIIQHENIFPNPRELEGPIYSRGG
SMEDYCDSTHGETTSAEDSTQDVTAEHHTSDDECEPIEAIAKFDYVGRTARELSFKKGAS
LLLYQRASDDWWEGRHNGIDGLIPHQYIVVQDTEDGVVERSSPKSEIEVMSEPPEEKVTA
RTGASCPTNHIFFNIYKSVLIMRLQSQGASMPERKAGDDSHGLGSSLTDSSSLGVGASCR
PSSQPIMSQNLPKEGPDKCSISGHGSLNSISRHSSLKNRMDSPQIRKTATAGRSKSFNNH
RPMDPEVIAQDIEATMNSALNELQELERQSSAKHTPDVVLDTLEPLKTSPVVAPTSEPSS
PLHTQLLKDPEPAFQRSASTAGDIACAFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSP
GVNSSASPQATDKSCTV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4012
Start on reference sequence 719
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable