DNASU Plasmid | OTTMUSG00000000720 (M. musculus )<br>In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Serpinb6e

Gene Name: serine (or cysteine) peptidase inhibitor, clade B, member 6e

Original Clone ID:

FLH270253.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00083179

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1134nts

Open reading frame: 1 to 1134

ATGGACTCTCTGCTGGAAGCAAATGCTACTTTTACCTTAAAGCTTTTTAGAGTATTGGGC
GAAGACAGCTCAAAAAATGTATTTTTCTCATCCTCGAGCATGTTTTCATCTCTGGCTCTC
ATCTTAATGGGAGCAAATGGAACCACTGCAAGCCAGATAAGTCAGGTACTTTCTTTGGAT
AAATGCAGCAATGGAGGTGCAGATGTCCAGCAGGGCTTCCAGTCACTTCTCACTGAAGTG
AACAAGACTGACACAGGGCACATGCTCAGAAGAGCCAACAAGATCTTCAGTGATAACAAT
TTTGACATCATGGAATCTTTTAAAGAGTCCTGCTACAAGTTATACAGAGTAGAGATAGAA
AAGTTGGACTTTAAGGGTACTCCGGAGCAGTGTCGACAGCACATCAACGCCTGGGTAGCC
AAAAAGACAAAAGATGTAATCAGAGAGTTGCTGTCTCTATATACAGTGAATTCAAACACT
AGGCTGATCCTTGTGAATGCCACCTACTTCAAAGGAAAGTGGGAGAAGCAGTTTAACAAA
GAGGACACCAGGGAGATGCCTTTCAAAGTCAGCAAGAATGAGAAGAAAACTGTTCAAATG
ATGTCTAAAAAGTCTACCTTCAAAACTTACTACGCAGAAGAGATATCTACTACTATTGTA
TTCCTTCCTTATACTGACAAAGAGCTGAGCATGATCATCATGCTGCCAGATGAACAAGTT
GAACTGAGTATGGTGGAAAATCAAATATCTTACAAGAAACTTATTCAGTGGACGAGGCTT
GTCAAGATGGAAGAAGAAGAAGTGCAGGTTTTCCTCCCACGGTTTAAGTTGGAGGCTACC
TATGACATGAAGGATGTCCTCTGCAAGCTGGGCATGACTGATGCCTTTGAGGAGAGCAGA
GCAGACTTTTCTGGAATATCTTCCAAGAAAGGCCTATTTCTGTCCAATGTTGTGCACAAA
AGTTTTGTGGAAGTCAATGAAGAAGGCACAGAGGCTGCAGTTGCCACTGAAATAGTCACA
GTGGGGTCACCACTGACTCAGCGGTGCTTAATTGCTGACCGTCCATTCCTTTTCCTCATT
CAAGGTGATAAAAGCAAAGAGATTCTGTTCCTTGGCCGGTTCTCTTCTCCCTAA

Protein sequence predicted based on nucleotide sequence

MDSLLEANATFTLKLFRVLGEDSSKNVFFSSSSMFSSLALILMGANGTTASQISQVLSLD
KCSNGGADVQQGFQSLLTEVNKTDTGHMLRRANKIFSDNNFDIMESFKESCYKLYRVEIE
KLDFKGTPEQCRQHINAWVAKKTKDVIRELLSLYTVNSNTRLILVNATYFKGKWEKQFNK
EDTREMPFKVSKNEKKTVQMMSKKSTFKTYYAEEISTTIVFLPYTDKELSMIIMLPDEQV
ELSMVENQISYKKLIQWTRLVKMEEEEVQVFLPRFKLEATYDMKDVLCKLGMTDAFEESR
ADFSGISSKKGLFLSNVVHKSFVEVNEEGTEAAVATEIVTVGSPLTQRCLIADRPFLFLI
QGDKSKEILFLGRFSSP*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 11

End on reference sequence 1144

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Serpinb6e

Symbol Nomenclature :

Serpinb6e

SYNONYM :

Gm11396; OTTMUSG00000000720; SPI3B; ovalbumin

Designation :

novel serine (or cysteine) proteinase inhibitor, clade B (Serpinb) member|serine (or cysteine) proteinase inhibitor, clade B, member 6e

Full Nomenclature :

serine (or cysteine) peptidase inhibitor, clade B, member 6e

GENEID :

435350

Locus Tag :

RP23-262J21.5

GI :

162319197

GenBank Accession :

BC156710

Mouse Genome Informatics :

2667778

Vega :

OTTMUSG00000000720

Target GenBank:

NM_001045535

Protein

SMART domain :

SERPIN : SERine Proteinase INhibitors

Clone

Cloning Information :

270253

HIP Master Clone ID :

201755

IMAGE ID :

100062207

Original Clone ID :

FLH270253.01X

Organism

Species Specific ID:

435350

Genome

Chromosome :

Map Location :

13 A3.3|13 13.98 cM

Ensembl :

ENSMUSG00000069248

Reagents

Labome :

Serpinb6e-siRNA

OTTMUSG00000000720 (M. musculus )In pENTR223.1 (Gateway donor/master vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Organism

Genome

Reagents

OTTMUSG00000000720 (M. musculus )
In pENTR223.1 (Gateway donor/master vector)