Gene:
Gene Symbol:
Nlrp2
Gene Name:
NLR family, pyrin domain containing 2
Original Clone ID:
FLH270257.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083183
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3141nts
Open reading frame: 1 to 3141
ATGGAACATTTTGATCCCCTGGGTTTCAACCTAAAAGACGTCCTGAGAAAGCTGGACAAG
ACTGAGTTGAACAATTTCAAGAGAACATTGAGGAGCTGTTCTCTGCCAGATACAATGAAA
CAGATCAACAAGCTGACAATGGACCTGGCTAATGGAGCTCAGCTAGCAGAAATTCTCACA
GACCACTGCCCCAGTGCTTGGATAAAGAGAGTCACCGTCCAGATCTTAGAGGAAATAAAC
CGTGTAGATCTAGCTGAGTTAGTAGTAAAGCAAATCGAAGAAGCAGTATTAAAGGTACCT
GAGGAAAAGGTTTCTTCTAAACCAAGAGAACCCTCGGGGACACTAACTTTCCCATGGAAT
TTTGTTCAAGGAGCTAAAAGGCCAGAGGACAAGCAAAAAGAGGAATGGAAGACTAGATAC
ACAGCTAAATGGAAGCAGAACTTCTGGCCTAAATGCAATAAAGAAATCTATGTGGTTACC
GAGAGCTACAAGACACTCCTGGCATTGTGTAACCCAAAGATAGAAACCCCATTTGCTCAT
GCAATTGTGCTGCATGGCCCTCCAGGCTCTGGGAAAACCACAATGGCAAAGCAGCTAATG
TTAGAATGGTCAGAGAGTAAGCAGGCCCAGATTTTCTCATGTGCCTTCTACATCAGCTGC
AGGGAAGTAAATAATACTAAACCTTGCACTTTTGCCCACCTCCTCTCCATGGACAATCCC
TCCTGGAGGGACTGTGTGATTCGAGATCTAATCCTGGGAAAGGAATTCTTATTTGTGGTA
GATGGTTTTGATGAGCTGACATTCCCAGCAGGAGCTCTGATCCGTGACCTTTGTGGTGAC
TGGAACACAGTGAAGCCTGTAGAGGTCTTACTGGGCAGTTTGCTGAAGAGGAAGATGGCA
CCTCATGCCACCCTGCTGGTAACCACACGGACACAGTCTTTGCACCAAATCTTTGTCATG
ATGGATCAGCCACTACTAGTAGAAACTCTGGGCTTCTTGGAGCAAGAAAAGCAGGAGTAC
TTTCAGAAATACTTTGAAGATGAGGAGGGTGAGGAGGAGGATAAAGGTGAGGGAAAGGCA
CTTAGAGCTCTCAAGGAAGTGAGATGCAATGCTGATCTATACCAAATGGCCTCACTTCCT
ACTGCATGTGGGATATTCTGCCTCTGTCTGGAGCTAAGGATGAAGAAGGGGGAAGACCTG
TCTCTGACATGTCAGACATACACCTCTATGTTCCTGAACTTCCTGTGCGAAGTCTTCTCA
TCAGAAACCTGTGAGGACCATCTCAATGAGGAATTCCAAATCTTATTCAAGAAAATATGT
ATCCTGGCTGCCAATAGTCTCTTGGAACAGGTGCCCATACTCTGTGAAGAAGACTTCTTG
ACATTAAAATTGAACCTAAACAATTTGCATCCCATGGTGTGCAGGCACATCCTCTTTAAG
GACAGTTCCAGTACACACTGTCTCTCTTTCATCTGCCTCGGTATCCAGCAACTCCTGGCT
GCAATAATATTTGTTCAGGAGCTTGGACAGGAAAGCAAGGGTGTCTCTAAATATAGTATA
CAGAACATGCTTTCAAGGGAAGCAAGATTAAAAAACCCTGACCTGTCTGGGCTGCTGCCA
TTTGTATTTGGCCTCCTGAATGAGACTCGTATTCAAGAGTTGAAGACTACTTTTGGCTGC
CAAATATCAACTGAGGTCAAGAGAAAATTCCTGGAGTGTGAGTCAGGGGAAAATAAACCC
TTACTATTACTGATGAATATGCAAGAAATCTTGTCCTGTCTCTATGAATCTCAGGAGGAG
GGGTTTGTGAAGGAAGCCATGGTCCTCTTTGAGGACATCTCCTTGCATTTGAAAACCAGC
ACTGACCTCATTCATGCTTCATTCTGCCTCAAGAACTCTCAGAACTTGCAGACAATGTCC
CTGAAAGTAGAAAAGGCGGTCTTTCCAGAGAATGTTGCTGCACTAGAGTCCACAGCTAAG
CATCAAAGGTCCCCGGATGAACAACGCATGTTAACTTTCTGGACAGACTTTTGTGACACA
TTTAATTCAAATAAGAAACTGGTATTTCTAGACATCCATGAAAGCTTCCTCAACAGCTCT
GCACTGGAGATTCTTTGTGAAAAGCTGCCCTCTGCTTCCTGTTGTCTCCAGAAAGTGGTC
CTCAAAAATATTTCCCCAGATGATGCCTACGAGAAATTGTGCCTCATTTTTAATGGTTAT
AAAACCATCTCACATCTGATCCTGCAAGGTGGCAACCTGGACAGCATGCATCACTCACTG
TGTGAGGTTCTGAAAAATCCAGCCTGCAACCTGAAGTTTCTCAGCCTGGGATCTTGTTCT
ACTGCTGCTCAGAAATGGGATGACTTCTTCCCAGTCCTGAAAGTCAATCAATCCCTGATA
TTTCTGGATCTCACAGACAACAGTCTCTTGGACAAAAGTGCTAAGCTCCTATGTAATATT
TGGAAGGAACCAAAGTGCATACTGCAGAGGGTATCGTTGGAGAACTGTCAGCTCACTGAA
GCCTGCTGCAAGGACCTCTCTTCCGTTTTGATGGTCAGCCGAACTCTGACACACCTCAGC
CTGGCCAACAATAAACTTGGGGATAACGGAGTGAAAAACCTGTGTGAAAGCATAAGCTGC
ACGGAGTGCAATCTACAGACCCTGGTGCTGTGGTCCTGTAACATCACCAACGCTGGTTGC
CACTATCTCTCAAAGATGCTCAAACAAACACTGAGCCTGAAACACTTGGATCTGGGGCTA
AATCGCATCGGAACCAAAGGAGCAAAGTTTCTGTGTGAGGCTTTGAAGAACCCCAAGAGT
AAATTGAAAAGTCTGTGGCTGTGTGGATGCTCCATCACTCCACTGAACTGCCAAGACTTC
TCTGAGACTCTTAGAAGCAACAAGAGCCTGAACACTCTAGACCTGTCTCAGAACGTCCTG
GGAACTGATGCCATAAAGACATTCTGTGAAGCCTTGAAACTTCAGATTTGCCCCCTGCAA
ATGCTTAGATTGAAATTTGATGAAGCTAAACCTAGTATCCAGAACCTGATCCAAGAGATG
AAGGTGAGCCATCCACAACTGAGAATCAGCAGTGACCAAGTGGATCTTAAGAATCCTCCT
CTTCCTCATTTCATTTTTTAA
Protein sequence predicted based on nucleotide sequence
MEHFDPLGFNLKDVLRKLDKTELNNFKRTLRSCSLPDTMKQINKLTMDLANGAQLAEILT DHCPSAWIKRVTVQILEEINRVDLAELVVKQIEEAVLKVPEEKVSSKPREPSGTLTFPWN FVQGAKRPEDKQKEEWKTRYTAKWKQNFWPKCNKEIYVVTESYKTLLALCNPKIETPFAH AIVLHGPPGSGKTTMAKQLMLEWSESKQAQIFSCAFYISCREVNNTKPCTFAHLLSMDNP SWRDCVIRDLILGKEFLFVVDGFDELTFPAGALIRDLCGDWNTVKPVEVLLGSLLKRKMA PHATLLVTTRTQSLHQIFVMMDQPLLVETLGFLEQEKQEYFQKYFEDEEGEEEDKGEGKA LRALKEVRCNADLYQMASLPTACGIFCLCLELRMKKGEDLSLTCQTYTSMFLNFLCEVFS SETCEDHLNEEFQILFKKICILAANSLLEQVPILCEEDFLTLKLNLNNLHPMVCRHILFK DSSSTHCLSFICLGIQQLLAAIIFVQELGQESKGVSKYSIQNMLSREARLKNPDLSGLLP FVFGLLNETRIQELKTTFGCQISTEVKRKFLECESGENKPLLLLMNMQEILSCLYESQEE GFVKEAMVLFEDISLHLKTSTDLIHASFCLKNSQNLQTMSLKVEKAVFPENVAALESTAK HQRSPDEQRMLTFWTDFCDTFNSNKKLVFLDIHESFLNSSALEILCEKLPSASCCLQKVV LKNISPDDAYEKLCLIFNGYKTISHLILQGGNLDSMHHSLCEVLKNPACNLKFLSLGSCS TAAQKWDDFFPVLKVNQSLIFLDLTDNSLLDKSAKLLCNIWKEPKCILQRVSLENCQLTE ACCKDLSSVLMVSRTLTHLSLANNKLGDNGVKNLCESISCTECNLQTLVLWSCNITNAGC HYLSKMLKQTLSLKHLDLGLNRIGTKGAKFLCEALKNPKSKLKSLWLCGCSITPLNCQDF SETLRSNKSLNTLDLSQNVLGTDAIKTFCEALKLQICPLQMLRLKFDEAKPSIQNLIQEM KVSHPQLRISSDQVDLKNPPLPHFIF*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3215
Start on reference sequence
75
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
