Gene:
Gene Symbol:
Hecw1
Gene Name:
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Original Clone ID:
FLH270270.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083196
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4773nts
Open reading frame: 1 to 4773
ATGATCTGTGCCCAGTTGGAGGCTGCCAAGAATCTGTACCAGAACAGGTTTTTAGGCCTG
GCTGCCATGGCGTCTCCATCTAGAAACTCCCAGAGCCGACGCCGGTGCAAAGAACCCCTC
CGGTACAGCTATAACCCAGACCAATTCCACAACATAGACATCAGAAATGGTGCCCATGAT
GCTATCACCATCCCTCGCTCCACCAGTGACACTGACCTGGTCACTTCAGACAGCCGCTCT
ACACTCATGGTCAGCAGTTCCTACTATTCGATAGGGCACTCCCAGGATTTGGTGATCCAC
TGGGACATCAAGGAAGAAGTGGATGCTGGAGACTGGATTGGCATGTACCTTATTGGTGAA
GTCTCATCTGAAAACTTTCTGGATTATAAGAACCGTGGAGTCAATGGTTCTCACAGAGGC
CAGATCATCTGGAAGATTGATGCCAGCTCTTACTTTGTGGAATCTGAAACTAAGATCTGC
TTCAAATACTACCACGGAGTAAGTGGGGCCCTGCGAGCTACAACCCCCAGTGTCACTGTC
AAGAACTCAGCAGAGATGGGAGGAAAAGAAAGAGGAATAGGAGACCAGGAAGGGTACCTA
TCGAGCCGGAGGCTGATCAGCTTCTCCCTCTCAGATTTCCAGGCCATGGGATTGAAGAAA
GGGATGTTTTTCAACCCCGACCCTTATCTGAAGATTTCCATTCAACCTGGGAAACATAGC
ATCTTCCCAGCCCTCCCTCATCATGGACAGGAAAGGAGATCCACGATCATAGGCAACACT
GTAAACCCCATCTGGCAGGCAGAGCATTTCAGTTTCGTGTCTCTGCCAACCGATGTGCTG
GAAATTGAAGTGAAGGACAAGTTTGCCAAGAGTCGTCCCATCATCAAGCGTTTCTTGGGA
AAGCTGTCAATGCCTGTTCAGAGGCTGCTGGAGAGACACGCCATAGGGGATAGGGTGGTC
AGCTACACACTTGGCCGCCGGCTTCCAACTGACCATGTGAGTGGGCAGCTGCAATTCCGA
TTTGAGATCACATCCTCCATCCATGCAGATGATGAAGAGATCTCTCTGAGTGCTGAGCCT
GAGTCGTCAGCAGAAACGCAGGACAGCATCATGAATAGTATGGTGGGAAACAGCAATGGA
GAGCCTTCTGGTGATGCTACAGAATTCTGCAAGGATGCGAAGCCAGAGTCACCAAGTGAA
GGGAATGGAGTAAACAGCTCAGAAAACCAAAACCAGGAGCATGCCGGGCCAGTTGAGGAA
GCGGCAGGAGCTATGGAGGCCAGAGATGGTAGCAATGTCTCTGAAGCACCCGAAGAGCCC
GGAGAGCTTCAGGACCCAGAGCAACATGACACCCAGCCCACCCTGAGTGCAGAAGAAGTG
GCCGAGGGACTTCCCCTGGATGAGGATTCGCCATCCTCGCTGTTGCCAGAAGAGAATACA
GCTCTAGGCAGCAAGGTAGAAGAGGAAACAGTACCAGAAAATGGAGCCAGAGAGGAAGAA
ATGCAGAAGGGGAAAGATGAAGAAGAGGAAGAAGAGGACGTTTCTACCCTGGAGCAAGGA
GAGCCTGGACTGGAGCTGAGGGTCTCCGTGAGAAAAAAAAGCAGACCATGTTCCCTACCT
GTATCAGAGCTTGAGACAGTGATTGCATCTGCCTGTGGAGATGCTGAGACCCCGAGAACC
CATTATATCCGCATCCACACCCTGCTGCACAGCATGCCCTCGGCCCAGAGAGGAAGCACC
ACGGAGGAGGAAGATGGCTTGGAGGAGGAATCCACCCTCAAGGAGTCCTCTGAAAAGGAT
GGGCTCAGCGAGGTGGACACAATAGCTGCTGACCCACAGTCCATGGAAGATGGCGAGAGC
GATGGGGCTACTCTGTGCATGGCACCCTCTGACTGCTCTGGGGGACATTTCTCCAGTTTA
TCCAAAGGCATTGGTGCTGGCCAAGATGGCGAAGCTCACCCCAGCACTGGGAGTGAGAGT
GACTCCAGCCCCCAGCAGGGAGCAGACCACAGTTGCGAGGGCTGTGATGCCTCATGCTGC
AGCCCGTCTTGCTATAGCACATCTTGCTACAGCAGCTCTTGTTACAGCAGCTCCTGTTAC
AGCAGCTCCTGCTACAATGGTAACAATCGGTTCGCCAGCCACACACGCTTCTCCTCTGTG
GACAGCGCCAAGATCTCTGAGAGCACAGTCTTTTCTTCACAAGAGGATGAGGAAGAGGAA
AACAGTGCATTCGAGTCGGTACCCGACTCGGTGCAAAGCCCAGAGCTAGATCCCGAGTCT
ACAAATGGGGCTGGCCCTTGGCAGGATGAACTGGCTGCCCCTGGTGGGAATGCAGCAAGA
AGTACTGAAGGTGAATGTCCTATACTCCATAATTCCCAGCCAATAAGCCAACTTCCTTCC
TTGAGGCCTGAACATCATCACTACCCAACAATCGATGAGCCTCTTCCACCAAACTGGGAG
GCTCGAATTGACAGCCATGGACGGGTCTTTTATGTGGACCATATAAATCGCACAACCACC
TGGCAGCGACCATCGATGGCCCCTACCCCCGATGGAATGATCAGATCCGGGTCTGTCCAC
CAGATGGAGCAGCTTAACAGAAGGTATCAAAACATCCAGAGGACCATGGCAACAGAGAGA
GCTGAAGAAGATTCTGGCAATCAAAACTCTGAGCAAATACCAGACGGAGGAGGAGGAGGT
GGTGGTGGGAGTGACTCGGAAGCAGAATCATCCCAATCAAGCTTAGATCTGAGGAGAGAA
GGGTCACTTTCTCCTGTCAACTCACAGAAGGTCACCTTGCTGCTGCAGTCTCCAGCGGTC
AAGTTCATCACCAATCCTGAGTTCTTCACTGTGTTGCATGCCAATTATAGTGCCTACAGG
GTTTTCACAAGCAGCACTTGCCTGAAGCATATGATTTTAAAAGTCCGAAGAGATGCTCGA
AATTTTGAGCGCTATCAGCACAACCGGGACCTGGTGAATTTCATCAACATGTTTGCAGAT
ACTCGGCTGGAACTGCCCCGGGGTTGGGAGATCAAAACTGATCATCAGGGAAAGTCTTTC
TTCGTGGACCACAACAGTCGAGCTACTACTTTCATAGATCCCCGAATCCCTCTTCAGAAT
GGCCGTCTTCCCAATCATCTAACCCACCGACAGCACCTCCAGAGGCTCCGGAGTTACAGT
GCTGGGGAGGCCTCAGAAGTCTCTAGAAACAGAGGAGCATCTTTGCTGGCCAGACCAGGA
CACAGCCTAATAGCTGCTATTCGAAGCCAACATCAACATGAATCCTTGCCACTGGCATAC
AATGACAAGATTGTGGCATTTCTTCGCCAGCCAAACATTTTTGAAATGCTACAAGAACGT
CAGCCAAGCTTGGCAAGAAACCACACACTCAGGGAGAAAATCCATTATATTCGGACAGAG
GGCAATCATGGGCTTGATAAATTGTCCTGTGATGCAGATCTAGTCATTTTGCTGAGTCTC
TTTGAAGAAGAGATCATGTCCTATGTTCCCCTGCAATCTGCCTTCCACCCTGGATATAGC
TTCTCTCCCCGCTGTTCACCCTGTTCCTCACCTCAGAACTCCCCAGGTTTACAGAGAGCC
AGCGCAAGAGCCCCTTCACCATACCGAAGGGATTTTGAAGCTAAGCTCCGCAATTTCTAC
CGAAAACTGGAAGCCAAAGGATTTGGACAGGGTCCGGGTAAAATTAAGCTCATCATCCGT
CGAGACCACTTGTTGGAGGGGACCTTCAATCAAGTGATGGCATACTCAAGGAAGGAGCTC
CAGAGAAACAAGCTCTACATCACCTTTGTTGGAGAGGAGGGGCTGGACTACAGTGGCCCT
TCTCGAGAGTTCTTCTTCCTGTTGTCTCAGGAGCTCTTCAACCCTTACTATGGACTCTTT
GAGTATTCTGCAAATGACACATACACAGTACAGATCAGCCCCATGTCAGCATTTGTAGAA
AACTATCTTGAATGGTTTAGATTCAGTGGTCGTATCCTGGGCCTAGCTCTGATCCATCAG
TACCTCCTGGACGCTTTCTTCACAAGACCCTTCTATAAGGGACTCCTGAAGCTGCCATGT
GATCTGAGTGACCTAGAATATTTGGATGAGGAATTCCACCAGAGTTTACAGTGGATGAAG
GACAACAACATCACAGACATTCTAGATCTCACCTTCACTGTTAATGAAGAAGTTTTTGGA
CAGGTAACAGAAAGAGAGTTGAAGTCTGGAGGCGCCAACACGCAGGTGACTGAGAAAAAC
AAGAAGGAATACATAGAGAGGATGGTGAAGTGGCGTGTGGAGCGTGGTGTGGTACAGCAG
ACGGAGGCATTGCTCAGGGGTTTCTATGAGGTTGTAGACTCAAGGCTCGTCTCAGTGTTT
GATGCGAGGGAACTGGAGCTGGTGATCGCTGGAACTGCTGAAATCGACCTGAATGACTGG
AGGAATAACACAGAGTACCGGGGAGGTTATCACGATGGCCACCTTGTGATCCGCTGGTTC
TGGGCTGCAGTGGAACGCTTCAATAATGAGCAGAGATTGCGATTACTTCAGTTTGTGACA
GGAACATCAAGTGTGCCCTATGAAGGCTTTGCAGCTCTGCGGGGAAGCAATGGGCTTCGA
CGTTTCTGCATAGAAAAGTGGGGGAAGATTACATCTCTTCCCAGGGCACATACGTGCTTC
AACCGATTGGATCTCCCACCATACCCTTCATATTCCATGTTGTATGAAAAGCTGTTAACA
GCAGTAGAAGAAACCAGCACCTTTGGACTTGAG
Protein sequence predicted based on nucleotide sequence
MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHD AITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIGE VSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTV KNSAEMGGKERGIGDQEGYLSSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHS IFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLG KLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHADDEEISLSAEP ESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSEGNGVNSSENQNQEHAGPVEE AAGAMEARDGSNVSEAPEEPGELQDPEQHDTQPTLSAEEVAEGLPLDEDSPSSLLPEENT ALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLELRVSVRKKSRPCSLP VSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEESTLKESSEKD GLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSES DSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSV DSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAAR STEGECPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTT WQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGG GGGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYR VFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSF FVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPG HSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTE GNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRA SARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKEL QRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVE NYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMK DNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQ TEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWF WAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCF NRLDLPPYPSYSMLYEKLLTAVEETSTFGLE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
376
End on reference sequence
5151
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
