Gene:
Gene Symbol:
Agap1
Gene Name:
ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Original Clone ID:
FLH270275.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083201
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2571nts
Open reading frame: 1 to 2571
ATGAACTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGCCGAGATCCAGCGCTTC
GAGTCGGTCCACCCCAACATCTACTCCATCTACGAGCTGCTGGAGCGCGTGGAGGAGCCG
GTGCTGCAGAACCAGATTCGGGAGCACGTCATCGCCATCGAAGATGCTTTCGTGAACAGC
CAGGAATGGACACTCAGCCGATCGGTACCGGAGCTCAAAGTGGGAATTGTGGGTAACCTG
GCCAGTGGCAAGTCTGCGCTGGTGCACCGGTACCTGACAGGCACGTACGTGCAGGAGGAA
TCTCCAGAAGGTGGCAGGTTCAAGAAGGAGATTGTTGTTGATGGACAGAGTTATCTGCTG
CTGATTAGGGATGAAGGGGGCCCCCCGGAGGCACAGTTCGCCATGTGGGTGGACGCGGTC
ATCTTTGTCTTCAGCTTGGAGGATGAGATCAGTTTCCAGACTGTCTACCATTACTACAGC
CGAATGGCCAACTACAGGAACACCAGTGAGATCCCATTAGTGCTGGTGGGGACCCAGGAT
GCCATAAGTTCCACCAACCCGAGGGTCATCGATGACGTCAGGGCTCGGAAGCTCTCCAAT
GACCTGAAGAGGTGCACGTACTATGAGACGTGCGCCACCTATGGGCTCAACGTGGAGCGG
GTCTTCCAGGACGTTGCCCAGAAGATCGTTGCCACAAGGAAGAAGCAGCAGCTATCCATA
GGCCCCTGCAAGTCCTTACCCAACTCTCCAAGCCACTCGTCTGTGTGTTCTGCCCAAGTG
TCCGCGGTACACATCAGCCAGACAAGTAACGGAGGTGGGAGTTTAAGTGACTACTCATCC
TCTGTTCCTTCGACCCCAAGCACCAGCCAGAAAGAGCTTCGGATCGACGTCCCTCCCACT
GCCAACACTCCAACGCCTGTCCGGAAGCAGTCCAAGCGCAGGTCCAACCTATTCACATCT
AGGAAAGGGAGCGACCCAGACAAAGAGAAGAAAGGCCTGGAGAGCCGTGCGGACAGCATC
GGGAGCGGGCGAGCCATCCCAATTAAACAGGGCATGCTGCTGAAGCGAAGTGGCAAGTCA
CTGAACAAAGAGTGGAAAAAGAAGTACGTCACCCTGTGCGACAACGGTGTGCTGACCTAC
CACCCCAGTTTACATGATTACATGCAGAATGTCCACGGCAAAGAGATAGACCTCTTGAGG
ACCACTGTGAAGGTGCCGGGGAAGAGGCCGCCCCGAGCCACGTCTGCCTGTGCACCGATC
TCCAGTCCCAAAACCAACGGCCTGGCCAAGGACATGAGCAGTTTACACATCTCACCAAAT
TCAGGGAATGTCACTAGTGCGTCTGGGTCTCAGATGGCAAGCGGCATCAGCCTGGTCTCC
TTCAACAGCCGACCCGATGGCATGCACCAGCGCTCCTACTCAGTCTCCAGTGCCGACCAG
TGGAGTGACGCTACAGTCATTGCAAACTCAGCCATCAGCAGTGACACGGGGCTGGGTGAC
TCGGTGTGTTCCAGCCCAAGTATCTCCAGCAGCACCAGCCCCAAGCTCGACCCACCCCCC
TCCCCCCACGCCAACAGAAAGAAGCACCGGAGGAAGAAAAGTACCAGCAACTTCAAGGCT
GACGGGCTCTCAGGCACTGCTGAAGAGCAAGAAGAAAACTTGGAGTTTATCATTGTGTCC
CTCACTGGCCAGACGTGGCACTTTGAAGCCACCACATACGAGGAGCGAGACGCATGGGTC
CAAGCCATCGAGAGCCAGATCCTCGCCAGCCTGCAGTCGTGCGAGAGCAGCAAGAATAAG
TCCCGACTAACCAGCCAGAGCGAAGCCATGGCCCTGCAGTCCATCCGTAACATGAGAGGG
AACTCGCACTGCGTGGACTGTGACACCCAAAACCCTAACTGGGCCAGTTTGAACTTGGGA
GCCCTCATGTGTATCGAGTGCTCGGGGATCCACCGGAATCTCGGCACCCACCTCTCTCGG
GTCAGATCGCTAGACCTGGACGACTGGCCCATGGAGCTGATCAAGGTGATGTCATCCATC
GGGAATGAGCTGGCCAACAGCGTGTGGGAAGAGGGCAGCCAGGGACGGACAAAGCCCTCA
CTGGACTCCACCAGGGAAGAAAAGGAAAGGTGGATCCGGGCCAAGTATGAGCAGAAACTC
TTCCTGGCCCCACTGCCATGCACAGAGTTCTCCCTGGGCCAGCAGCTGCTGCGTGCCACT
GCCGAGGAAGACCTGCGGACTGTCATCCTACTTCTGGCACATGGGTCCCGGGACGAGGTG
AATGAGACCTGTGGGGAAGGAGACGGTCGCACGGCGCTGCACCTGGCCTGTCGCAAGGGC
AACGTGGTCCTGGCTCAGCTGCTGATCTGGTACGGAGTGGACGTCATGGCCCGAGACGCC
CACGGGAACACAGCACTGGCCTATGCCCGGCAGGCCTCCAGCCAGGAGTGTATTGACGTG
CTTCTACAGTATGGCTGCCCCGACGAGCGCTTTGTGCTCATGGCTACCCCTAACCTGTCC
AGGAAGAGCAACAGCAGGAACAACAGTAGCGGGAGGGCGCCCAGTGTCATC
Protein sequence predicted based on nucleotide sequence
MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERVEEPVLQNQIREHVIAIEDAFVNS QEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLL LIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQD AISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSI GPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPT ANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKS LNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPI SSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQ WSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKA DGLSGTAEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNK SRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSR VRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKL FLAPLPCTEFSLGQQLLRATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKG NVVLAQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLS RKSNSRNNSSGRAPSVI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3193
Start on reference sequence
620
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
