Gene:
Gene Symbol:
Rasgrf2
Gene Name:
RAS protein-specific guanine nucleotide-releasing factor 2
Original Clone ID:
FLH270283.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083209
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3567nts
Open reading frame: 1 to 3567
ATGCAGAAGAGCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCATGCTGGCGCGC
AAGGAGGGCACCAAACGCGGCTTCCTGAGCAAGAAGGCTGCAGAGGCGAGCCGCTGGCAC
GAGAAGTGGTTCGCTCTCTACCAGAATGTGCTCTTTTACTTCGAAGGCGAGCAGAGCGGC
CGGCCAGCGGGCATGTACCTTCTAGAGGGCTGCAGCTGTGAGCGGACGCCTGCACCACCC
AGGACCAACGCAGGACCCGCAGGCGCCCGGGACGCTTTGGACAAGCAGTATTACTTCACT
GTCCTTTTTGGGCACGATGGTCAAAAACCACTGGAGCTGCGCTGTGAGGAGGAGCAGGCC
GGGAAAGAGTGGATGGAGGCCATTCACCAAGCCAGTTACGCGGACATTTTGATCGAGAGG
GAAGTGCTAATGCAGAAGTACATTCATCTAGTTCAGATTGTGGAGACCGAGAAGATCGCC
ACCAACCAACTCCGACATCAACTCGAAGATCAAGATACCGAAATCGAAAGGCTTAAATCA
GAGATTGTTGCTCTTAATAAGACCAAGGAACGGATGCGTCCGTACCACGTCCATCAAGAG
GAGGAGGATCCAGACATCAAGAAGATTAAGAAGGTTCAGAGCTTCATGAGAGGGTGGCTG
TGTAGGAGGAAGTGGAAGACCATCGTGCAGGACTACATTTGTTCTCCCCATGCAGAGAGC
ATGAGGAAGAGAAACCAGATTGTGTTCACGATGGTGGAGGCCGAGACGGAGTATGTACAC
CAGCTTTACATCCTGGTGAACGGTTTCCTCAGGCCCCTGCGCATGGCAGCCAGCTCCAAG
AAGCCCCCCATTAACCACGATGACGTCAGCAGCATTTTTCTCAACAGTGAAACAATCATG
TTCCTTCATGAAATATTCCATCAAGGACTAAAGGCAAGGCTGGCAAATTGGCCTACTTTG
GTTTTAGCGGATCTATTTGATATTTTGCTGCCCATGCTGAACATTTATCAAGAATTTGTA
CGTAATCACCAGTACAGCCTCCAAGTACTTGCCAACTGTAAGCAAAACAGAGATTTTGAC
AAGCTCTTAAAGCAGTATGAAGCCAACCCTGCCTGTGAGGGGAGGATGCTGGAGACGTTC
TTGACCTACCCAATGTTTCAGATTCCCAGGTATATCATCACGCTGCACGAACTCCTGGCC
CACACCCCCCATGAACATGTGGAGAGGAAAAGTCTGGAGTTCGCTAAGTCCAAGCTGGAG
GAACTGTCCAGAGTGATGCATGATGAAGTCAGTGACACCGAGAACATACGGAAAAATCTC
GCCATTGAGAGGATGATCGTAGAGGGCTGTGACATTTTGCTGGACACCAGCCAAACTTTT
ATCCGACAAGGTTCCCTTATTCAAGTGCCTTCTGTGGAGAGGGGGAAACTGAGTAAAGTC
CGCCTGGGCTCGCTGTCCCTGAAAAAGGAAGGCGAGAGACAGTGTTTCTTATTCACGAAG
CACTTTCTGATTTGTACACGAAGTTCAGGAGGAAAGCTGCATTTGCTCAAGACAGGTGGG
GTTCTCTCCCTCATTCAGTGTACATTGATTGAGGAGCCCGATGGAAGTGACGATGACCCC
AAAGGTTCTGGCCACATGTTTGGACATCTGGATTTTAAAATCGTAGTGGAGCCTCCTGAC
GCTGCCTCTTTCACTGTGGTCTTATTGGCACCTTCACGCCAGGAGAAGGCTGCATGGATG
AGCGACATCAGTCAGTGTGTGGACAATATACGATGTAACGGATTGATGACTATAGTCTTT
GAAGAGAATTCCAAAGTTACTGTGCCACATATGATTAAGTCTGATGCCCGTCTTCACAAA
GACGACACTGACATCTGTTTCAGTAAAACACTCAATTCCTGCAAAGTGCCCCAGATCCGC
TACGCCAGCGTGGAGCGCCTCTTGGAGCGGTTGACGGACTTGCGGTTTCTGAGCATTGAT
TTCCTCAACACCTTCCTGCACACCTACCGCATTTTCACCACGGCCACCGTAGTGCTGGCA
AAGCTCTCAGACATCTACAAGAGGCCTTTCACCTCCATTCCTGTCAGGTCATTAGAGTTA
TTTTTTGCCACAAGCCAGAACAACAGAGAACATTTGGTAGATGGAAAATCCCCACGTCTC
TGTCGCAAGTTCTCTTCCCCACCTCCCCTGGCTGTGTCCAGAACATCCTCCCCAGTGAGG
GCCAGAAAGCTGTCTTTGACGTCTTCCTTGAACTCAAGGATTGGAGCCTTGGACCTGACT
AATTCCTCATCTTCCAGTAGTCCCACCACCACCACCCACAGCCCTGCTGCATCCCCACCA
CCGCACACTGCTGTCTTGGAGTCTGCACCAGCCGACAAAGCGGGAGATTCTGCAGACATG
TCACCATGTAGATCCCCCACAACTCCCCGGCACCTCCGCTATCGGCAGCCTGGAGGACAG
GTAGCCGACAGCGCCCACTGTTCTGTGTCACCTGCTTCTGCTTTTGCAATCGCCACAGCT
GCAGCAGGACATGGGAGTCCACCAGGATTTAACAACGAGAGAACATGTGACAAGGAGTTC
ATCATACGGAGAACAGCCACCAATCGAGTCCTGAATGTCCTCCGCCACTGGGTCTCAAAG
CATGCCCAGGATTTTGAACTCAATAATGAACTCAAAATGAATGTTCTAAACTTGCTAGAA
GAAGTTCTACGAGACCCGGACCTTCTTCCTCAAGAAAGGAAAGCAACGGCGAACATCCTG
AGGGCTCTTTCACAAGATGATCAAGATGATATCCACCTAAAATTAGAGGATATAATTCAA
ATGACAGACTGTCCAAAGGCCGAGTGCTTTGAGACGTTGTCAGCCATGGAGCTGGCCGAG
CAGATCACTCTCCTGGACCACATTGTTTTCAGAAGCATCCCCTACGAAGAGTTTCTTGGG
CAGGGCTGGATGAAGCTTGACAAGAACGAAAGGACGCCTTACATCATGAAAACCAGCCAG
CACTTCAATGAGATGAGTAACCTGGTGGCCTCCCAGATAATGAATTATGCCGACATCAGC
TCCCGTGCCAACGCCATTGAGAAGTGGGTGGCAGTGGCGGACATTTGCCGATGTCTCCAC
AACTACAATGGTGTTCTGGAGATCACCTCAGCCTTAAACAGAAGTGCCATCTACAGGCTG
AAGAAAACCTGGGCCAAGGTGTCCAAGCAGACAAAAGCTCTAATGGACAAACTTCAGAAG
ACTGTTTCATCTGAAGGAAGATTTAAAAACCTCAGAGAGACTCTCAAAAACTGTAACCCC
CCAGCTGTCCCTTACCTTGGGATGTACTTGACAGATCTGGCATTCATTGAAGAAGGGACA
CCGAACTTTACCGAAGAGGGCCTTGTCAATTTCTCCAAAATGCGAATGATATCACACATT
ATTCGAGAGATACGCCAGTTCCAGCAGACCGCTTACCGCATAGACCAGCAGCCAAAGGTC
ATACAATACCTGCTTGACAAAGCCCTTGTCATAGACGAAGATTCACTCTATGAGCTGTCA
CTCAAAATTGAACCTCGACTCCCTGCT
Protein sequence predicted based on nucleotide sequence
MQKSVRYNEGHALYLAMLARKEGTKRGFLSKKAAEASRWHEKWFALYQNVLFYFEGEQSG RPAGMYLLEGCSCERTPAPPRTNAGPAGARDALDKQYYFTVLFGHDGQKPLELRCEEEQA GKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIATNQLRHQLEDQDTEIERLKS EIVALNKTKERMRPYHVHQEEEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAES MRKRNQIVFTMVEAETEYVHQLYILVNGFLRPLRMAASSKKPPINHDDVSSIFLNSETIM FLHEIFHQGLKARLANWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFD KLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLE ELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKV RLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIQCTLIEEPDGSDDDP KGSGHMFGHLDFKIVVEPPDAASFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVF EENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSID FLNTFLHTYRIFTTATVVLAKLSDIYKRPFTSIPVRSLELFFATSQNNREHLVDGKSPRL CRKFSSPPPLAVSRTSSPVRARKLSLTSSLNSRIGALDLTNSSSSSSPTTTTHSPAASPP PHTAVLESAPADKAGDSADMSPCRSPTTPRHLRYRQPGGQVADSAHCSVSPASAFAIATA AAGHGSPPGFNNERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLE EVLRDPDLLPQERKATANILRALSQDDQDDIHLKLEDIIQMTDCPKAECFETLSAMELAE QITLLDHIVFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNEMSNLVASQIMNYADIS SRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQK TVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHI IREIRQFQQTAYRIDQQPKVIQYLLDKALVIDEDSLYELSLKIEPRLPA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3570
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
