Gene:
Gene Symbol:
Cntnap5a
Gene Name:
contactin associated protein-like 5A
Original Clone ID:
FLH270292.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083218
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3912nts
Open reading frame: 1 to 3912
ATGGATTCTGTACCAAGACTGAACAGCGTTTTGACTTTGGTGCTCTCAGGCTTGTGGCAT
TTAGGATTAACAGCGACAAACTACAACTGTGATGACCCATTGTCCAACTTCCTCTCACTG
AAGGCTTTCTCCAGTTCCTCAGATATCACTGGAAGTTCTAGTTCAGCTCAACTGAACTGG
AGAATGGGAACTGGTGGTTGGTCCCCAGCAGATTCCAATGCCCAACAGTGGCTCCAGATT
GACCTGCAAAACAGAGTGGAGATTACAGCTGTAGCCACACAGGGAAGATATGGAAGCTCT
GACTGGGTCACAAGCTATCGCCTGATGTTTAGTGACACAGGGCACAACTGGCAACCATAC
AATCAAGAAGACAGCATCTGGACATTTGTAGGAAACATGAATTCTGACAGTGTGGTTCAC
CACAAGCTGCTGCACTCTGTGAGAGCCCGCTTTGTTCGCTTTGTGCCCCTAGAGTGGAAC
CCGAATGGAAAGATTGGCATGAGAGTGGAGGCCTATGGATGCTCCTATAGATCAGATGTA
GCTGACTTTGATGGCCGGAGCTCACTTTTATACAGGTTCAATCAGAAGACAATGAGTACC
CTCAAAGATGTGATCTCCCTGAAGTTCAAGAGCATGCAAGGAGATGGAGTTCTGTTTCAT
GGAGAAGGACAACGTGGAGACCACATCACTCTGGAACTCCAGAAGGGCAGGCTAGCTCTC
TACCTGAACCTGGATGACAGCAAAGCTCGGCTCAGCAGCACTGTGCCCTTGGTCATCCTG
GGTAGCCTTCTGGATGACCAGCACTGGCATTCAGTTCTCCTGGAGCGTGTGGGCAAGCAG
GCAAACTTCACTGTGGACATGAACACTCAGCACTTCCGGACCAAGGGTGACACAGATTCT
CTGGACATTGACTATGAGCTCAGTTTTGGAGGAATTCCAGTACCAAGCAAACCTGGGACA
TTTTTAAAGAAAAACTTCCATGGCTGTATTGAAAACCTTTACTACAATGGAGTGAACATA
ATTGATTTGGCTAAAAGACGCAAGCATCAGATCTACTCAGGCAATGTCACTTTTTCCTGC
TCAGAACCACAAATTGTTCCTATCACATTTATCAACTCCAGGAGCAGTTATTTGATGCTG
CCTGGAACCCCCCAAATTGATGGTCTGTCAGTGAGTTTCCAGTTCCGAACATGGAATGAG
GATGGTCTGCTTCTGTCCACAGAGCTGTCTGAAGGCTCAGGGACCCTGCTGCTCATACTG
GAGGGAGGGACTCTGAGGCTCCTGATTAAGAAAGTGGCAAGACATGGAACTGAAATCCTC
ACAGGCAGTGGCTTAAATGATGGCCTGTGGCATTCTGTCAGCATCAATGCCAGAAGAAAT
CGTGTTACTCTCACACTGGATAATGATGCTGCTTCCCCAGCTCCAGACACCTCCCGAATA
CAGATTTATTCTGGAAAGAGTTACTACTTTGGAGGGTGCCCTGACAATCTCACCGATTCC
CAATGCTTAAACCCCATTAAGGCTTTCCAAGGCTGCATGAGGCTCATCTTTATTGATAAC
CAGCCCAAGGACCTCATTTCAGTTCAGCAAGGTTCCCTGGGGAGTTTTAGTGATTTACAC
ATTGATCTGTGTAGCATCAAAGACAGGTGTTTGCCAAACTACTGTGAACATGGAGGACAT
TGTGACCAAACCTGGACTACCTTCTACTGCAACTGTAGTGGTACTGGTTACACTGGAGCC
ACCTGCCATGACTCCATCTATGAGCAATCCTGTGAGGTGTATAGACACAGAGGGCACACA
GCTGGGTTCTTCTATGTTGACTCTGATGGCAGTGGGCCACTGGGACCCCTTCAGGTGTAC
TGCAATATTACTGAGGACAAGATATGGATGACAGTTCAGCACAACAACACAGAGCTGACT
CGGGTACAGGGTTCCAGTCCTGAGAATCCCTACTCCATGACCTTAAACTATGGTGGCAGT
ATGGAGCAGCTGGAAGCTTTGATTGATGGCTCAGAATACTGTGAACAGGAGGTGATATAC
CACTGTAGGAGATCCCGTCTGCTTAACACACCAGATGGAGCCCCATTCACCTGGTGGATT
GGGAGGTCCACTGAAAGGCACCCTTACTGGGGAGGTGCTGTTCCGGGAGTCCAGCAGTGT
GGATGTGGCCTGGAGGAAAGCTGCCTGGACAGTCGACACTTTTGCAATTGTGATGCAGAT
ATAGATGAATGGGCAAATGATACTGGCTTGTTTTCCTTCAAAGATCACTTGCCTGTAACT
CAGATAATTATCATGGATACCAACCGAACAAACTCAGAAGCTGCTTGGAGAATTGGGCCT
TTGCGTTGCTATGGTGACAGGCACTTCTGGAATGCCGTCTCATTTTCTACGGAAGCGTCT
TACCTCCACTTTCCCACCTTCCATACGGAATTCAGCGCTGACATTTCTTTCTTTTTCAAA
ACCACCGCTTTATCTGGAGTTTTTCTAGAAAACCTTGGCATTAAAGACTTCTTCCGACTT
GAAATGAGCTCCCCTTCTGAGGTCACATTTGCCATTGATGTTGGAAACGGCCCCATCGAG
CTCCTCGTCCAGTCTCCTTATCCTCTGAATGACAATCAATGGCATTACATCCGAGCTGAG
ACAAACCTCAAGGAGACCTCACTTCAGGTGGACAACCTTCCACAGAGTATGAGGGAGGCC
TCAGAGGGAGGCCATTTCCGACTTCAGTTGAATAGCCAGTTGTTTGTAGGAGGAACATCA
TCAAGACAGAAAGGCTTTCTTGGATGCATCCGTTCTTTACTCTTGAATGGGCATAAAGTA
GACCTGGAAGAGAGGGCAAAGGTCACGTCTGGAGTCAGACCTGGTTGTCCAGGTCACTGC
AGCAGCTATGGAAGCAATTGCCACAATGGGGGCAAGTGTGTGGAGAAACATACTGGTTAT
TCCTGTGACTGTACCAACTCACCGTATGAAGGACCTTTCTGCAGGAAAGAAATTTCAGCT
CTTTTTGATTCTGGCACATCTGTAACATACATGTTTCAAGAACCATATCCAGTGACCAAG
AACACAAGCCTTTCCTCTTCAGCTATCTACACAGATTTAGCTCCATTCAAGGAAACAATC
ATGCTGAGTTTCATGACAACACAAGCCCCCACCCTCTTGCTCTACCTCAATTTTTCTTCT
CAGAATTTTCTGGCTATCCTGCTCTCCAGGAATGGAAGTTTACAGGTTCGATATCGACTA
AGCAAGGATGAAAGTCATGTATTCAACATGGACACAGAGAATCTGGCCAACAGAAGAGTG
CATCAGGTGAAGATTAGCCGAGATGGTCCAGAACTTTCCATTCAGATGGACCAACAACTC
TTCAGCTATAGCTTCTCCCCAGAAGTTGAGTTCCGGACACTGAGGTCACTTGTCCTAGGC
AAAGTCACAGAAACTCTTGATTTGGATCCAGAAGTTGCTCGAGCGAACACCTTGGGTTTT
GTTGGATGCCTTTCTTCAGTCCAATACAATCACATAGCACCATTGAAGGCAGCCCTCCGC
CATGCCAGTATTGCACCTGTGACTGTCCAAAAGACCTTGACAGAATCCAGCTGTGGCTCC
ATGGTGGACTCTGATGTGAATGCAGTGACCACCGTGCATTCTTTGTCAGATTCCTTTGGA
AAGACAGATGACCATGAACCACTCCAAAATGCAGTCCGAAGTGACTCAGCAGTCATTGGA
GGCGTCATAGCAGTGGTGACATTTATTACTTTTTGTGTCATTGGAATCATGACCCGCTTC
CTCTATCAACATAAGCAGTCACACCGCACCAATCAGATGAAGAAGGAATACCCAGAGAAC
TTGGACAATTCCTTTAGAAATGAAATTGACTTGCAAAACACAGCCACTGAATGCAAACGA
GAATATTTCATC
Protein sequence predicted based on nucleotide sequence
MDSVPRLNSVLTLVLSGLWHLGLTATNYNCDDPLSNFLSLKAFSSSSDITGSSSSAQLNW RMGTGGWSPADSNAQQWLQIDLQNRVEITAVATQGRYGSSDWVTSYRLMFSDTGHNWQPY NQEDSIWTFVGNMNSDSVVHHKLLHSVRARFVRFVPLEWNPNGKIGMRVEAYGCSYRSDV ADFDGRSSLLYRFNQKTMSTLKDVISLKFKSMQGDGVLFHGEGQRGDHITLELQKGRLAL YLNLDDSKARLSSTVPLVILGSLLDDQHWHSVLLERVGKQANFTVDMNTQHFRTKGDTDS LDIDYELSFGGIPVPSKPGTFLKKNFHGCIENLYYNGVNIIDLAKRRKHQIYSGNVTFSC SEPQIVPITFINSRSSYLMLPGTPQIDGLSVSFQFRTWNEDGLLLSTELSEGSGTLLLIL EGGTLRLLIKKVARHGTEILTGSGLNDGLWHSVSINARRNRVTLTLDNDAASPAPDTSRI QIYSGKSYYFGGCPDNLTDSQCLNPIKAFQGCMRLIFIDNQPKDLISVQQGSLGSFSDLH IDLCSIKDRCLPNYCEHGGHCDQTWTTFYCNCSGTGYTGATCHDSIYEQSCEVYRHRGHT AGFFYVDSDGSGPLGPLQVYCNITEDKIWMTVQHNNTELTRVQGSSPENPYSMTLNYGGS MEQLEALIDGSEYCEQEVIYHCRRSRLLNTPDGAPFTWWIGRSTERHPYWGGAVPGVQQC GCGLEESCLDSRHFCNCDADIDEWANDTGLFSFKDHLPVTQIIIMDTNRTNSEAAWRIGP LRCYGDRHFWNAVSFSTEASYLHFPTFHTEFSADISFFFKTTALSGVFLENLGIKDFFRL EMSSPSEVTFAIDVGNGPIELLVQSPYPLNDNQWHYIRAETNLKETSLQVDNLPQSMREA SEGGHFRLQLNSQLFVGGTSSRQKGFLGCIRSLLLNGHKVDLEERAKVTSGVRPGCPGHC SSYGSNCHNGGKCVEKHTGYSCDCTNSPYEGPFCRKEISALFDSGTSVTYMFQEPYPVTK NTSLSSSAIYTDLAPFKETIMLSFMTTQAPTLLLYLNFSSQNFLAILLSRNGSLQVRYRL SKDESHVFNMDTENLANRRVHQVKISRDGPELSIQMDQQLFSYSFSPEVEFRTLRSLVLG KVTETLDLDPEVARANTLGFVGCLSSVQYNHIAPLKAALRHASIAPVTVQKTLTESSCGS MVDSDVNAVTTVHSLSDSFGKTDDHEPLQNAVRSDSAVIGGVIAVVTFITFCVIGIMTRF LYQHKQSHRTNQMKKEYPENLDNSFRNEIDLQNTATECKREYFI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3915
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
