Gene:
Gene Symbol:
Pappa
Gene Name:
pregnancy-associated plasma protein A
Original Clone ID:
FLH270295.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083221
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4872nts
Open reading frame: 1 to 4872
ATGCGGCTCTGGAGTTGGGTGCTGCGCCTGGGGCTGCTGAGCGCCGCGCTGGGCTGCGGG
CTGGCCGAGCGCCCCCGCCGGGTCCGAAGAGACCCTCGGGCCGTGCGCCCCCCGCGCCCC
GCCGCTGGACCGGCCACCTGCGCCACCCGGGCGGCCCGCGGTCGCCGCGCCTCGCCGCCG
CCGCCTCCGGGCGGTGCCTGGGAAGCCGTGCGCGTCCCCCGGCGGCGGCAGCAGCGGGCG
GCGAGGGGCGCCGAGGAGCCGAGCCCGCCTAGCCGGGCGCTCTATTTCAGCGGGCGAGGG
GAGCAGCTGCGCCTCCGGGCCGACCTGGAGCTACCCCGCGACGCCTTTACACTGCAAGTG
TGGCTGCGAGCCGAGGGTGGCCAGAAGTCTCCAGCAGTGATCACAGGGCTGTATGACAAA
TGTTCTTATACCTCACGTGATCGAGGATGGGTCATGGGCATTCACACCACCAGTGATCAA
GGCAACAGAGATCCACGCTACTTTTTCTCCTTGAAGACAGACCGGGCCAGGAAAGTGACC
ACCATTGATGCCCATCGCAGCTACCTCCCAGGTCAGTGGGTACATCTAGCTGCTACCTAT
GATGGGCGGCTGATGAAGCTCTATATGAATGGTGCCCAGGTGGCAACTTCGGCTGAGCAA
GTAGGTGGCATATTCAGCCCACTGACCCAGAAGTGTAAAGTGCTCATGTTGGGGGGCAGT
GCTCTGAATCACAACTTCCGGGGCCACATTGAACACTTCAGTCTATGGAAAGTAGCAAGA
ACCCAGCGAGAGATTGTATCCGACATGGAAACCCGTGGCCTCCACACCCCTCTACCTCAG
CTCCTCCTCCAGGAGAACTGGGACAACGTGAAGCGCACTTGGTCCCCCATGAAGGATGGC
AACAGCCCCCAGGTGGAATTCAGCAATGCCCACGGCTTCCTGTTGGACACTAATTTGGAG
CCCCCTCTTTGTGGGCAGACACTGTGTGACAACACAGAAGTCATCTCCAGTTACAATCAG
CTCCCAAGTTTTCGGCAGCCCAAGGTGGTCCGCTATCGTGTGGTCAACATCTATGATGAT
CACCATGAGAATCCAACGGTGAGCTGGCAACAGATTGACTTTCAGCACCAACAGCTGGCT
GAGGCCTTTCAACACTACAACATCTCCTGGGAGCTGGAGGTACTGAATATAAACAGTTCC
TCTCTGCGTCACCGCCTCATCCTAGCCAACTGTGACATCAGCAAGATTGGGGATGAAAAA
TGTGATCCAGAATGTAACCATACACTGACTGGTCATGATGGTGGAGATTGCCGCCAGCTG
CGCTACCCTGCGTTCATGAAGAAGCAGCAGAATGGTGTGTGTGACATGGACTGTAACTAC
GAAAGGTTTAATTTTGATGGTGGAGAGTGCTGTGACCCAGACATCACTGATGTCACTAAG
ACATGCTTTGATCCTGACTCTCCACACAGAGCCTACTTGGATGTTAATGAGCTAAAGAAC
ATTCTTAGACTGGACGGATCAACACATCTCAATATTTTCTTTGCAAACTCTTCAGAGGAG
GAGTTGGCAGGAGTGGCAACTTGGCCATGGGACAAGGAAGCCCTAATGCACTTGGGCGGT
ATTGTCTTGAACCCATCTTTCTATGGCATTCCCGGACACACCCACACCATGATTCATGAG
ATTGGGCATAGCCTGGGCCTCTATCACATCTTCCGTGGCATCTCAGAAATCCAGTCCTGC
AGTGATCCCTGCATGGAGACAGAGCCTTCATTTGAAACTGGAGACCTCTGCAATGATACC
AACCCAGCCCCCAAACACAAGTTTTGTGGAGACCCTGGACCAGGGAATGACACTTGTGGC
TTTCATGGCTTCTTCAACACTCCTTACAACAACTTCATGAGCTACGCAGATGACGACTGT
ACAGACTCTTTCACGCCCAATCAAGTCTCCAGAATGCACTGTTACCTGGACCTCGTATAC
CAGAGCTGGCAGCCCTCCAGAAAGCCAGCACCTGTAGCTCTTGCGCCCCAGGTTGTGGGG
CACACAATGGACTCTGTGATGCTAGAGTGGTTCCCACCCATCGATGGCCACTTCTTTGAA
AGAGAATTGGGATCAGCATGTGACCTTTGCCTAGAAGGGAGAATCCTGGTGCAATATGCT
TTCAATGCCTCCTCCCCCATGCCCTGTGGACCGTCAGGACACTGGAGTCCTCGGGAAGCA
GAAGGTCACCCAGATGTTGAACAGCCCTGTAAATCCAGTGTCCGAACCTGGAGTCCAAAT
TCAGCTGTCAACCCACACACAGTTCCTCCAGCCTGCCCTGAGCCACAAGGCTGCTACCTC
GAGCTGGAATTTCGCTACCCTTTGGTCCCTGAGTCTCTGACCATCTGGGTAACCTTTGTC
TCCAGTGATTGGGACTCTAGTGGAGCTGTCAATGACATCAAACTCTTGACTATCAGTGGA
AAGAATATCTCTTTGGGTCCTCAGAATGTTTTCTGTGATATCCCACTTACCATCAGACTC
CGGGATGTGGGTGAGGAGGTATATGGCATCCAAATCTATACTCTTGATGAGCACCTGGAG
ATTGATGCAGCAATGCTGACCTCCACTGTAGACAGTCCACTCTGCCTACAGTGTAAACCT
CTGCAGTATAAAGTGCTTCGAGACCCACCTCTGCTAGAAGATGTAGCCTCATTACTCCAC
CTCAACAGAAGATTCATGGACACGGATCTGAAACTTGGCAGTGTGTACCAGTACCGGATT
ATCACCATTTCAGGAAATGAAGAGAGCGAGCCATCACCTGCTGCCATATACACCCACGGA
AGTGGGTACTGTGGTGATGGCGTTATCCAAAAAGACCAAGGAGAAGAATGTGACGACATG
AATAAGGTCAATGGGGATGGCTGCTCCCTTTTCTGCAAGCAAGAAGTTTCCTTCAACTGC
ATTGATGAACCCAGCCGGTGCTATTTCCATGATGGGGATGGGATGTGTGAAGAGTTTGAA
CAAAAAACTAGCATTAAAGACTGTGGTGTCTACACGCCCCAGGGTTTCCTGGATCAGTGG
GCATCCAATGCTTCAGTATCTCATCAAGACCAGCAGTGCCCAGGTTGGGTTGTCATTGGG
CAGCCAGCGGCATCTCAGGTGTGTCGAACCAAGGTGATAGATCTCAGTGAAGGCATTTCC
CAGCATGCTTGGTATCCTTGCACCATTACTTACCCATACTACCATCTGCCTCAGACCACA
TTCTGGCTCCAGACATATTTCTCTCAGCCAATGGTTGCTGCAGCTGTAATTATTCACCTG
GTGACTGATGGGACATACTATGGGGACCAAAAGCAAGAGACCATCAGTGTGCAGTTGCTT
GATACCAAAGATCAAAGCCATGATCTAGGCCTCCATGTCTTGAGCTGCAGAAACAATCCC
CTGATTATCCCTGTGGTCCATGACCTCAGCCAGCCCTTCTACCACAGCCAGGCGGTACAT
GTGAGCTTCAGTTCGCCCCTGGTCGCCATCTCGGGGGTGGCCCTCCGCTCTTTCGACAAC
TTTGACCCCGTCACCCTGAGCAGCTGCCAGAGAGGAGAGACCTACAGCCCTGCTGAGCAG
AGCTGTGTGCATTTTGCCTGTCAAGCTGCCGACTGCCCAGAACTGGCCGTGGGGAATGCT
TCTCTCAACTGTTCCAGCAACCACCACTACCATGGTGCCCAGTGCACTGTGAGCTGCCAG
ACAGGTTATGTGCTGCAGATACAGCGGGACGATGAGCTAATCAAGAGCCAGGTAGGGCCA
AGCATCACAGTGACATGTACCGAGGGCAAATGGAACAAGCAGGTGGCATGTGAGCCGGTG
GACTGTGGTATCCCAGATCACCATCACGTCTATGCTGCCTCCTTCTCCTGTCCAGAGGGT
ACCACCTTTGGTAGAAGATGTTCTTTTCAGTGTCGCCACCCTGCCCAGCTGAAAGGCAAC
AACAGCTTTCTGACCTGTATGGAAGATGGACTGTGGTCCTTCCCAGAGGCCTTGTGTGAG
CTCATGTGCCTCGCCCCACCCCCAGTTCCCAATGCGGACCTACAGACAGCCCGGTGTCGA
GAGAACAAGCACAAGGTGGGCTCCTTCTGCAAGTACAAGTGTAAACCTGGATACCACGTG
CCTGGCTCATCTCGGAAGTCCAAGAAACGGGCTTTCAAGACTCAATGTACTCAAGATGGC
AGCTGGCAAGAGGGAACTTGTGTGCCGGTGACTTGTGACCCACCTCCACCCAAATTCCAT
GGGCTCTATCAATGCACTAATGGCTTCCAGTTCAATAGTGAGTGCAGGATCAAGTGTGAA
GACAGTGATGCCTCCCAGGGCCGTGGGAGCAATATCATTCACTGCCGGAAAGATGGCACT
TGGAGTGGTTCCTTCCACGTCTGCCGAGAGATGCAAGGCCAGTGCTCAGCCCCAAACCAA
CTCAACAGTAACCTCAAATTGCAGTGTCCTGATGGCTATGCAATAGGGTCAGAGTGTGCC
ATCTCGTGCCTGGACCATAACAGCGAGTCCATCATCCTTCCCGTTAACTTGACAGTGCGT
GACATACCCCATTGGATGAACCCCACACGAGTACAGAGGATTGTCTGCACTGCTGGTCTC
CAGTGGTATCCCCACCCTGCTCTGATCCACTGTGTCAAAGGCTGTGAGCCATTCATGGGA
GACAACTACTGTGATGCCATCAACAATCGAGCCTTCTGCAACTATGATGGTGGGGACTGC
TGCACCTCCACAGTAAAGACCAAAAAGGTCACTCCCTTTCCTATGTCCTGTGACCTACAA
AATGACTGCGCCTGTCGAGACCCTGAGGCCCAAGAACACAACCGGAAAGATCTTCGGGGA
TATAGCCATGGC
Protein sequence predicted based on nucleotide sequence
MRLWSWVLRLGLLSAALGCGLAERPRRVRRDPRAVRPPRPAAGPATCATRAARGRRASPP PPPGGAWEAVRVPRRRQQRAARGAEEPSPPSRALYFSGRGEQLRLRADLELPRDAFTLQV WLRAEGGQKSPAVITGLYDKCSYTSRDRGWVMGIHTTSDQGNRDPRYFFSLKTDRARKVT TIDAHRSYLPGQWVHLAATYDGRLMKLYMNGAQVATSAEQVGGIFSPLTQKCKVLMLGGS ALNHNFRGHIEHFSLWKVARTQREIVSDMETRGLHTPLPQLLLQENWDNVKRTWSPMKDG NSPQVEFSNAHGFLLDTNLEPPLCGQTLCDNTEVISSYNQLPSFRQPKVVRYRVVNIYDD HHENPTVSWQQIDFQHQQLAEAFQHYNISWELEVLNINSSSLRHRLILANCDISKIGDEK CDPECNHTLTGHDGGDCRQLRYPAFMKKQQNGVCDMDCNYERFNFDGGECCDPDITDVTK TCFDPDSPHRAYLDVNELKNILRLDGSTHLNIFFANSSEEELAGVATWPWDKEALMHLGG IVLNPSFYGIPGHTHTMIHEIGHSLGLYHIFRGISEIQSCSDPCMETEPSFETGDLCNDT NPAPKHKFCGDPGPGNDTCGFHGFFNTPYNNFMSYADDDCTDSFTPNQVSRMHCYLDLVY QSWQPSRKPAPVALAPQVVGHTMDSVMLEWFPPIDGHFFERELGSACDLCLEGRILVQYA FNASSPMPCGPSGHWSPREAEGHPDVEQPCKSSVRTWSPNSAVNPHTVPPACPEPQGCYL ELEFRYPLVPESLTIWVTFVSSDWDSSGAVNDIKLLTISGKNISLGPQNVFCDIPLTIRL RDVGEEVYGIQIYTLDEHLEIDAAMLTSTVDSPLCLQCKPLQYKVLRDPPLLEDVASLLH LNRRFMDTDLKLGSVYQYRIITISGNEESEPSPAAIYTHGSGYCGDGVIQKDQGEECDDM NKVNGDGCSLFCKQEVSFNCIDEPSRCYFHDGDGMCEEFEQKTSIKDCGVYTPQGFLDQW ASNASVSHQDQQCPGWVVIGQPAASQVCRTKVIDLSEGISQHAWYPCTITYPYYHLPQTT FWLQTYFSQPMVAAAVIIHLVTDGTYYGDQKQETISVQLLDTKDQSHDLGLHVLSCRNNP LIIPVVHDLSQPFYHSQAVHVSFSSPLVAISGVALRSFDNFDPVTLSSCQRGETYSPAEQ SCVHFACQAADCPELAVGNASLNCSSNHHYHGAQCTVSCQTGYVLQIQRDDELIKSQVGP SITVTCTEGKWNKQVACEPVDCGIPDHHHVYAASFSCPEGTTFGRRCSFQCRHPAQLKGN NSFLTCMEDGLWSFPEALCELMCLAPPPVPNADLQTARCRENKHKVGSFCKYKCKPGYHV PGSSRKSKKRAFKTQCTQDGSWQEGTCVPVTCDPPPPKFHGLYQCTNGFQFNSECRIKCE DSDASQGRGSNIIHCRKDGTWSGSFHVCREMQGQCSAPNQLNSNLKLQCPDGYAIGSECA ISCLDHNSESIILPVNLTVRDIPHWMNPTRVQRIVCTAGLQWYPHPALIHCVKGCEPFMG DNYCDAINNRAFCNYDGGDCCTSTVKTKKVTPFPMSCDLQNDCACRDPEAQEHNRKDLRG YSHG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
369
End on reference sequence
5243
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
