Gene:
Gene Symbol:
Myo3b
Gene Name:
myosin IIIB
Original Clone ID:
FLH270308.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083234
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3999nts
Open reading frame: 1 to 3999
ATGGCAGCAGCCTCGGAGGTGAGCGGTGAGCACACAGGAGACAAAACCGGGAAGCACCTG
TATGGATTATTTCACTATAACCCCATGATGCTTGGCCTGGAATCACTCCCCGATCCCATG
GAGACCTGGGAGATTATAGAGACGATTGGCAAAGGCACTTATGGCAAGGTCTACAAGGTA
GCCAACAAGAGAGATGGGAGCCTGGCTGCCGTGAAGGTTCTGGACCCCGTCAGTGATATG
GATGAGGAGATTGAGGCTGAATACAACATTCTGCAGTTTCTCCCCAGCCATCCCAACGTT
GTAAAGTTTTATGGGATGTTTTACAAAGCCGATCGCTGTGTGGGAGGACAGCTATGGCTG
GTTCTGGAGCTGTGTAATGGGGGCTCTGTCACTGAGCTTGTCAAGGGCCTTCTGAGGTGC
GGCAAGAGGCTGGACGAAGCTGTGATTTCCTACATTCTGTATGGAGCCCTCTTGGGCCTT
CAGCATTTGCACTGCCACCGAATCATCCACCGAGATGTGAAGGGGAATAACATTCTTCTG
ACGACAGAAGGAGGAGTTAAGCTCGTTGACTTTGGTGTCTCTGCTCAACTTACAAGCACA
CGGCTGCGGAGAAACACATCAGTTGGGACCCCATTCTGGATGGCTCCTGAGGTCATTGCT
TGCGAGCAGCAGTATGACTCGTCCTATGACGCTCGTTGTGACGTCTGGTCCTTGGGCATC
ACAGCCATTGAGCTGGGAGATGGAGACCCTCCCCTCTTTGAAATGCATCCTGTGAAAATG
CTCTTTAAGATACCAAGAAACCCTCCACCCACTTTGCTGCACCCTGACAGCTGGTGTGAG
GAATTTAACCACTTCATTTCACAGTGTCTTATTAAAGATTTTGAAAAGCGGCCTTCGGTC
ACCCATCTCTTGGACCACCCATTCATTAAAGGAACTCAAGGCAAGGTTTTGTGTCTGCAA
AAACAGCTGGCCAAGGTGCTCCAGGACCAGAAGCATCGAAACCCTGTTGCTAAAACCAGG
CATGAAAGGATGCATACTGGAAGACCCCATCGAGTAGAGGATGCCGGCAAGTGCTGCCTT
GAGGATGACCTGGTCAATCTCGAGGTCCTGGACGAGGACACGATTATCTACTGGCTGCAG
AAGCGGTATGCAGATGCACTGATCTACACCTATGTTGGAGACATTCTAATTGCCTTAAAC
CCCTTCCAGAATCTAAGTATATACTCTCCACAGTTTTCCAGGCTTTATCATGGGGTGAAA
CGTTCCTCGAATCCCCCCCACATATTTGCATCAGCAGACAACGCTTATCAGTGTTTGGTT
ACTTTCAGCAAAGACCAGTGTATTGTCATCAGTGGAGAAAGCGGATCGGGGAAGACGGAA
AGCGCCCATCTGATTGTTCAGCATCTTACTTTCTTAGGAAAGGCTGACAATCAGACCCTA
CGCCAGAAAATCCTCCAAGTGAACTCCCTGGTGGAAGCCTTCGGGAACGCCCGCACAGCC
ATCAATGATAACTCCAGCCGCTTCGGGAAGTATCTAGAAATGATGTTCACACCAACTGGA
GCTGTCATGGGGGCAAGAATCTCCGAGTATCTTCTTGAGAAATCCAGAGTTATACAGCAG
GCAGCGGGAGAGAAAAATTTTCATATATTTTACTATATTTATGCTGGTCTTTATCACCAA
AAGAAACTCGCTGAGTTCAGACTTCCCGAGGAAAAGCCTCCTAGATATATAGCTGGGGAA
ACTGAAAGAGTCATGCAGGACATCACCTCCAAAGAGTCTTACCGGACACAGTTTGAGGCC
ATTCAGCACTGCTTCAAGATTATTGGATTCGCTGACAAGGAGGTACACTCTGTGTACAGA
ATTCTGGCGGGGATTTTGAATATTGGCAGCATTGAATTCGCAGCTATTTCCTCTCAACAC
CAAACTGATAAAAGTGAGGTACCTAATCCAGAAGCTCTGGAAAACGCTGCCTGCGTGCTC
TGCATTAGCTCCGAAGAGCTTCAGGAGGCCCTCACTTCCCACTGTGTGGTCACACGAGGG
GAGACCATTGTCAGAGCCAACACAGTGGACAGGGCTGAAGACGTCAGGGACGCCATGTCT
AAGGCCCTCTATGGGAGGCTGTTCAGCTGGATTGTGAATCGAATCAACACACTCCTGCAG
CCAGATAAAAACATATGTAGTGCAGAGGACAGGATGAATGTGGGCATCTTGGACATTTTT
GGCTTTGAGGATTTCCAGAGGAATTCCTTTGAACAGCTCTGCATAAATATTGCCAATGAA
CAGATCCAGTACTACTTCAATCAGCATGTGTTTGCTCTTGAGCAGATGGAGTACAAGAAT
GAAGGTGTCGATGCTGTCCTCGTGCAGTATGAAGACAATCGCCCACTCCTGGACATGTTC
CTCCAAAAACCCTTGGGCCTTCTGGCACTTCTGGATGAAGAAAGTCGATTTCCCCAAGGG
ACAGACCAGACCTTGGTTGATAAATTTGAAGACAACCTACGGTGTAAGTTCTTCTGGAGG
CCCAAAGGTGTGGAACTCTGTTTTGGCATTCAGCACTATGCTGGACCGGTGTTATACGAT
GCTTCTGGGGTTCTTGAGAAAAACAGAGACACCCTCCCTGCTGACGTGGTGGTAGTCCTG
AGGACGTCTGAGAACAAACTCCTTCAGCAACTGTTCTCAATTCCTTTGACCAAAACAGGT
AATTTGGCACAAACCAGAGCTAAGATCACAGCATCCTCGAGATCTTTGCCTCCACATTTC
AGTGCTGGGAGGGCCAAGGTAGACACCCTGGAGGTGATTCGACATCCAGAAGAAACCACC
AACATGAAGAGACAAACCATGGCTTCTTACTTCCGGTATTCTCTGATGGACCTGCTCTCC
AAAATGGTGGTCGGACAGCCTCACTTTATTCGTTGCATTAAGCCTAATGATGACCGCAAG
GCCCTGCAGTTCTCCCAAGACAGAGTCTTGGCCCAGCTTCGCTCCACGGGCATCCTGGAG
ACCGTCAGCATCCGCAGGCAGGGCTACTCCCATCGCATCTTCTTTGAAGAGTTTGTTAAA
AGGTATTATTACTTGGCATTCCGAGCACATCAAACACCTCCTGCTAACAAAGAGAGCTGT
GTTGCTATCTTGGAAAAGTCCAGATTAGATCACTGGGTCCTGGGGAAAACAAAGGTTTTC
CTCAAATATTACCATGTCGAGCAATTAAATTTGCTTCTGCGAGAAGTCATGGGACGAGTG
GTCATGCTGCAGGCATATACCAAGGGCTGGCTCGGAGCAAGGAGATACAAGAGAGCCAAA
GAGAAGAGAGAGAAGGGCGCCATCACCATCCAGTCAGCCTGGAGAGGCTATGATGCTCGG
AGGAAATTGAAGCAAAGAAGCCGGAGGAGGAGTGAGTCCGAGGCTCACATTCACACAGTT
CTCCAAACCACTCCTGATCAGAAATACTGTCCAGATTCAGGAGGAGAGTCCAACAGAGGT
CATGAAGAGACGTCAAGGAACTGCCCCGCTGAAGCCGACACAGATGGGCACCCACAAGCC
CAGAGTCCCCCAACAGGCTGTGATGTCACCTCAGGACATGCAGATACAGCAGCTGGGTAT
ACTGTTGCTGAGCTCTCAGTTGCTGGAACCGATGTGTCACCTTCTCTGGTGTACCACACT
GCCTCAGCTCACCAAAGACTGAGCCCCTGTGAAGACTCTCTCAAACCTGGTTCCGAAGAA
GGTCTCTCCCAGAAGCAGCGAGCACCTCGCAGACGGTGTCAGCAGCCCAAAATGTTGAGT
AGCCCCGAGGACACCATGTACTATAACCAGTTAAATGGAACTCTAGAATATCAAGGGAGC
CAGAGGAAGCCAAGAAAACTTGGACAAATCAAAGTGCTGGATGGGGAGGACCAGTATTAC
AAATGCCTGTCCCCTGGGGCCTGTGCCCCTGAGGAAACCCACTCAGTCCACCCTTTCTTC
TTCTCCTCGTCACCCAGAGAAGACCCCTTTGCTCAACAC
Protein sequence predicted based on nucleotide sequence
MAAASEVSGEHTGDKTGKHLYGLFHYNPMMLGLESLPDPMETWEIIETIGKGTYGKVYKV ANKRDGSLAAVKVLDPVSDMDEEIEAEYNILQFLPSHPNVVKFYGMFYKADRCVGGQLWL VLELCNGGSVTELVKGLLRCGKRLDEAVISYILYGALLGLQHLHCHRIIHRDVKGNNILL TTEGGVKLVDFGVSAQLTSTRLRRNTSVGTPFWMAPEVIACEQQYDSSYDARCDVWSLGI TAIELGDGDPPLFEMHPVKMLFKIPRNPPPTLLHPDSWCEEFNHFISQCLIKDFEKRPSV THLLDHPFIKGTQGKVLCLQKQLAKVLQDQKHRNPVAKTRHERMHTGRPHRVEDAGKCCL EDDLVNLEVLDEDTIIYWLQKRYADALIYTYVGDILIALNPFQNLSIYSPQFSRLYHGVK RSSNPPHIFASADNAYQCLVTFSKDQCIVISGESGSGKTESAHLIVQHLTFLGKADNQTL RQKILQVNSLVEAFGNARTAINDNSSRFGKYLEMMFTPTGAVMGARISEYLLEKSRVIQQ AAGEKNFHIFYYIYAGLYHQKKLAEFRLPEEKPPRYIAGETERVMQDITSKESYRTQFEA IQHCFKIIGFADKEVHSVYRILAGILNIGSIEFAAISSQHQTDKSEVPNPEALENAACVL CISSEELQEALTSHCVVTRGETIVRANTVDRAEDVRDAMSKALYGRLFSWIVNRINTLLQ PDKNICSAEDRMNVGILDIFGFEDFQRNSFEQLCINIANEQIQYYFNQHVFALEQMEYKN EGVDAVLVQYEDNRPLLDMFLQKPLGLLALLDEESRFPQGTDQTLVDKFEDNLRCKFFWR PKGVELCFGIQHYAGPVLYDASGVLEKNRDTLPADVVVVLRTSENKLLQQLFSIPLTKTG NLAQTRAKITASSRSLPPHFSAGRAKVDTLEVIRHPEETTNMKRQTMASYFRYSLMDLLS KMVVGQPHFIRCIKPNDDRKALQFSQDRVLAQLRSTGILETVSIRRQGYSHRIFFEEFVK RYYYLAFRAHQTPPANKESCVAILEKSRLDHWVLGKTKVFLKYYHVEQLNLLLREVMGRV VMLQAYTKGWLGARRYKRAKEKREKGAITIQSAWRGYDARRKLKQRSRRRSESEAHIHTV LQTTPDQKYCPDSGGESNRGHEETSRNCPAEADTDGHPQAQSPPTGCDVTSGHADTAAGY TVAELSVAGTDVSPSLVYHTASAHQRLSPCEDSLKPGSEEGLSQKQRAPRRRCQQPKMLS SPEDTMYYNQLNGTLEYQGSQRKPRKLGQIKVLDGEDQYYKCLSPGACAPEETHSVHPFF FSSSPREDPFAQH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
21
End on reference sequence
4022
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
