Gene:
Gene Symbol:
Pkdrej
Gene Name:
polycystic kidney disease (polycystin) and REJ (sperm receptor for egg jelly homolog, sea urchin)
Original Clone ID:
FLH270309.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083235
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6378nts
Open reading frame: 1 to 6378
ATGTGGCCGGGCCCCGCGCTGCTGCTCCTGGGCCTGGGCCTGGGTCTAGGCAGCCAGCCG
CCACCCACCGGCCCTCGCGGGCTGCCGGGCGTGCTCAGGGGCGCCCCGGGGTTGGGGCAG
GGCGCGGAGAGCAGCGTCCGGGGCGGTGACACCGGGGGCCTGTCCCCGCGTGCAGCCCCG
CGCCACGCCAGCCCTACGCCGCCCCGTAGGTGTCCCTCGGGCGCAGCTGCACGCGTCCTC
CTGAAAGTCAACTCTTCGGACCCCGCAGCCGCCAAGGCTAACGTGTCCTGCCAGACGGCC
CCCTGTATCATGCAGCCAGTGAAGATCAACAGGAAGGACCAGAACGCTCCCCTTATCCTG
AGCAGAGACGAGGAAGCCACCCTCAATATCACTGTGCGTTGGTACTGTCCTGATGCCTTG
GTCATTCGCAAGAGCTGGGTGTACTTTTCGGTGGCCTCTGTGAACGACACCCCTGATTGG
AACCGACCTGTGCTCTTGCCGCAGGTGGCAGTGTCTAAGGGTTTCTCCCTCCACATCCCC
AAATATGCCCTGCCTTACGGGGTGTATGTGTTTAACTTTACGCTGTCCATCTTCAGGTGG
GACCTCGCCTGGCCCACGGTGACAGGCTCGGATATCATCTACATTTGGGTTAGAAAACGC
CCCCTGAAGGCTGTTCTTCTTGGAGCCCCCAGAGTAACCGTGAAGTTCTCCGATGAACTG
ATTCTGAATGGAAGCATGTCCTACGACCCAGAGGCAGACATCCCCACATGGGGACTCCAG
TTTTTTTGGTATTGTACCACAAATCCAAGATACTATAGTGGCAACTATGTATTAGTGATC
AACCAGGCTGTCTGCCACCCAGAGCAGGATTCACTGAACTGGCCTTGGGCAGTGGGCTCT
GTACTAACCATTCCACCAAAAACTCTTAGGGGCAATGGTGTGTATTACTTCAGAATGGTG
GTACAGAAGCAAAACAGAACTGCATTTTCTGATAAAACTGTACACGTGCTCCAGGGGCTT
CAGCCCAAGGCACACATTTCCTGCATTGAAAATTGTGGCCCCACGTTAGGTGTGTCAGAT
AGATTTTCTCTGTTTCTGAACTGCCCAAGTTGTGGCCGCCAGGATTTGTATAGCTGGTCC
ATTTTGTCTCTAACGGGCCATGAAGTGATGTTTGACTGGGCAGGGCAAGCAATCACCAGG
AGGAATGGACCTTATTTGTCCATAAAAGCTTTTGCCTTTAGGAATTTTTTGGAAAATAGG
GTTTGGGTTTCCCTGATTCTGAAATCTTGGAGTGGTATGACCACGACCTTGAGACATCCC
GTTGTCATTAACCATGGCCCTCGAGTTGGCAAATGCAAGATAAATCCAGCTAGTGGGATT
TCCATGGTTACTAAATTTGCTGTTGAATGTAGTGATTTTAAGGATGAGAACCTTCCCCTT
AAGTATAAAATAATAGTATCTGAGTTGGACAGCATTGGCGCGATCAGTTCCGTAGAAGAG
AATACACTGGGTTCCGTGGTGTATTCAGGGGCAGAGCCGATAACACCAGCCTTCTTTCTC
CCTGTCGGTACGGTGACAGATGAGTACAATGTGAGGCTTTATGTTCAGGTCTATGACTCC
TTAGGGACATTTTCTCAAGTGACTTTGCAAGCGACTGTGACGGCACCTACTGTCAGGGAC
TCTTCAAAGGATGTTTTACAACAGCTGCTCAACGTCACCATGAAACCGACGTCACTGTTG
TCTACCTTGCTTCAAAAGCAGGACTGGCTACAGGCTGGCTACTTAACCTATGTGGTGATT
TCTGTCTTGAATAACATAAAAGGGGAGCAGGAACTTCAAGATGACAGAGCCCGTCTCCTG
GAGCACCTCGTCAAACAGTCTCTCACGTTTGCTATGAACACAGCGGATGAGATCGGTCAG
TCGGTGATGGTGATTACCAAATTAACCCAGAAAGCCTCTGACTTGAGTCAAGCGGATTTA
GAGGCCACCTCGTTCCGGCTTAGGCAAGCAAGCCAAGACCTACAAGAATACCAGCACAGA
CACAAGCACCTTCAGCAGGTTGAAGTTGTGGGCACTGGCATCCTAACCAGTCTGTCTAAC
CTTCTTAAGCTGATTAATCCCTACTATACGTTACAAGATCCTTTGTTTGTAGTCGAATCA
CTATCAGACACAATACTGGCTAACAAGGTTCCGGGGAGCAAGACCACTGCTCTGAGGACC
AGTAACTTTAACATGTATGTGGAGAAAGTTGAAAACTGGAATGTTTTTAAGGCCTTCAGA
AATGATTCACTCTGTCCAAACTGCCTCCGTGCGACACTGAACGCAAGCACGGTCCCCAGG
CTACCCGCAAAGGCTCCGATTTCTATGATGTTTTGTGAGTTCGCAGACGACCCCTTTCCT
TGGTTGACTTACCCGGAAAACATTTCTGTAGATGTGGTTGGGTTCAGGATGACTGGAGTC
GCAGATAACAATAAGGTCATAGAGATCACACCCGACATAGCAGAAGTGTACCTGGTCAGA
AAAGATCTGACCCCTTCGAGTTTTAACCTTACGGTGGGACCCGGCATTAAAAGCGATGGG
TTCTCAAAGGTGACAACGGGTGGAATTAGCTTTGAGGTAGACAGCAGGTGGACTGGGGAG
CTACTCATTCACATTGTCACAAATGTGACAGTGTTGTTTGAGGCCTTGGTATATGAAGGC
TGTCAGCTCACGCCCACCAATCTGATGGCCACTTTCCTTGTTCCTAATGACATCCCTCCC
ATTGTCAAGTGGAGTGGCCTGTTTGACCCAACCTGTTCAGTCAAGGAGGCCCGCGTGGTT
TGTCTGTCATCATCCCTGCTTCGAAGCATAGCTCAGCGCAGATTTTCATTCAAGTATAAC
ATATCCATGGTCCTGCAGGCATCGCGTTTTGTCCTGAAGCCCACTAACAAGCTAGTGAGA
ATTGCTCTCTTTATGGTTCACTGTTTGGACATGTATGGGATCCAGAGTGATTGGCAACAA
AGTACCTGCGTGCTGGGTGAGAAGACCACGTGGAAAACAGTACACTGTGTCTGTAGGAAT
GGAAGGCGGAGCCGGCGGCAGCTGACTTCTGTGAAGCTGACCTACCATCATCTGCACACC
CACTTTGTGACTGCTAAGGTAATTGTGGTCCCTAACCCCGTGGATCTGAGGCTAGCGATC
ATTAGTAACCTTACCCAAAACCCTGCGACTTTCCTGGCCGTGCTTTTCATTATGATCTTG
TATGCAATTCTGGCTTTCTGGGCCTTGCACAGGGATGTGATAGATCTGTATTTTCGGGAC
AATGTGGTAATACTGACTGATAATGATCCTTTTGATACCCTATGCTATCTAGTAACCATC
TTTACTGGGAGTCGCTGGGGGTCTGGAACCAGAGCCAATGTTTTTATCCAGCTTATGGGA
ACGGAGGGTACCAGTGATGTTCACTGTTTAAGCCATCCATATTTTAAAACCCTATACCGA
GGAAGTATCAATACTTTCCTCCTGACAACAAAAAATGACTTGGGGGATATCCATTCCATC
CGAGTGTGGCATGACAATTCGGGCGAAGCACCTAGCTGGTACCTAAGTCGAATCAAAGTA
GAAAATCTCTTCAACAAGCGCATTTGGCTTTTTGTGTGCCGGAGGTGGCTCTCTGTGGAC
ACCACTTTGGATGCAACGTTTTCTGTCACCAACCCGGATGAGCCTCTGAAAAGGACAGAC
TTCTTCATGATTGATGTGAGGGATAAGCTTCGGAAGAACCACATGTGGATCTCCATTTTC
ACGGAAATTGTCCCCAAACCATTCAACAGGCTCCAGAGGCTGTCCTGCTGCTTGGCCATG
CTGCTAAGCTCTCTTGTTTGCAATATCATGTTTTTTAACTTGAACCAAAAGGAGAAAATT
GAGTCCAGACACATGCACATTATCAGGTCAATGCTGATAGGCATCGAGAGCGTCGTAATC
ACAATCCCCGTGCAATTACTGATAACTTTTTTCTTCACCTATTCCCAGAAGAATCTTAAA
ATGAATCTAGACAAAGTGGCACCCCAGAAGCACCCCCTGATGTCAGAGGAAGGTCTGTCC
TGGAAAGAGCGATTGCACAAATGGCATGAATATGAAATGAAGGCTCTCCCCAGGAGAGCT
GCCGTGTCCACCTCTGCTCCCGAGGAAAAGGAAGCCTTTGAAACCTCACAGAAGCATGAG
AAAGCAGACACCCAAATGTCCAATAAGAACAGCAGTAACAATAACCAAGAAGCCAGTGAA
GGTGTCCCTCCGAAAGCTTTCTCTTCCCAACCGCATACTACTGAGTCTGTTCAAAAGAAG
ACCCAGATTATCCTGCCCAGGTGGTGTGTTTACATAGCCTGGTTTTTGGTGTTTGCCACT
TCCGGTATATCGTCATTCTTTATTGTATTTTACGGCGTGACTTACGGCTATGCCAAGTCA
ATAGAGTGGCTCTTTGCGTCGTTCTGTTCCTTCTGTCAGTCTGTGTTCCTCGTACAACCA
TGCAATATTCTCCTGAGGTCAGGCACCAGGAGCTACAAGCCCAAGTACTGTAAGAACCTC
TCGTGGTCCAGCAAGTACCATTACTCAGAAATCAGGCTGCAGGGGTTGACGATGACACAG
GAGGAGATGGAGCAGCTTCATGAGGACATCGCCTACGTCCGAAGCTTGAGCATGTACCAG
CCCATCACTGAGGACAAAATCCAGATCCTCAGGCGGGAGAACAGGATCCGGAGGAGGTCC
TTCTTGTTCCTGAGCTACCTCGTGACTCACTTCATATTCCTGACCCTTCTCCTGTTGCTC
ATTTTCTCACTGCGCCACAACGACAGCTTCTACTATAACCAGTTTATTCGCCATCGGTTC
TCCGTGGATCTCGCCACTGTGATGAAGCTGGGAGATATCTACACCTGGCTCCACGGCGTG
TTCCTCCCTTTGTTACACAATGACCCAAACCCAACATTCCTTCCGGACAGCTCTTCTAAA
ATCCTTGGCCTTCCGCTTGTGAGGCAAGTGAGGGCCAGACCTAGCAACAAGACGTGTCTG
TTGGCCAAGAAGTTTGTACAGAGCAGCGTTGCAGGGGAAATCCATTGTCACCCACAGTAC
GGCATTGACCCAGAAGACACACAACACTATTCCAGTGTCTGGAGTAAGGCTGGCAAGCAG
TCAACAGACAAAGCTAGCCATGGGTTTACTTATAAGCCTCCGGGGAAAAGATGGGTGTAT
CATTCCTATGGAGTACTAAACACCTATGGATCTGGAGGGTATGTGTTCTACTTCTTTCCA
GGACAGCAGATGTTTAATTCTACAGTGAGGCTCAAGGAACTCGAGGGAAAGAATTGGCTG
GATGAGTTGACATGGGCCGTGATTGTGGAACTGACCACTCTGAATCCAGACACCAGTCTG
ATGTGTAGCATTTCGGTTGTATTCGAAGTCTCTCCCTTAGGGGTTGTCAACTCTAGCCTT
TCTGTGTACTCCTTCTCGCTGGCTGACTTCAACAGAAAAACCTCGTCAGAAATCTACCTC
TACGCCGCCATTCTTATTTTCTTTTGTGCCTATGTTGTGGACGAGGGCTACATCATCAGG
CAAGAGAGGGCTTCCTACATAAGGAGTGTGTACAACCTGCTCAACTTCTCTCTCAAGTGC
ATGTTCGCTCTCCTGATAGTGCTTTTCTTCTGGAAGTACTTCCTGGCCACCAAAATGGTC
CAGCTTTACCTTGCTGACCCCGAAGCCTTCATTCCCTTCCATGCCGTTTCTCGGGTTGAC
CACTTCATGAGAATCATCTTGGCTTTCCTGTTGTTTCTGACCATACTGAAGACACTCAGA
TATTCCAGGTTCTTCTATAATGTGCGCCTGGCTCAGAAGGCCATCCAGGCTGCCCTTCCT
GGCATCTGCCACACGGCTTTGGTGGTGTCTATATATTCCTTCATGTACGTAGCTTTTGGG
TACTTGGTGTTCGGTCAGCATGAATGGAATTACAGCAATATGATTCACGCCACCCAAACC
ATCTTCTCCTACTGCGTGTCAGCCTTTCAGAACACAGAATTTTCCGGCAACAAGGTTCTC
GGGGTCCTGTTTCTGTCGTCTTTCATGTTGGTGATGATCTGTATCTTCATCAACTTGTTT
CAGGCAGTGATTCTGTCTGCCTATGATGAGATGAAGCAGCCTGTGTACGAGGAGCCGTCT
GATGAGGCCGAGGCTGTAACCTATCTGTGCAACAGGCTAAAATCTGGGTTTGACTTTCTC
ACCACCCGATCTAGAGACAAAGACCAATCCAACTTCTTCGTCGACATGCTCTATGGGCAG
CCGGAGAAGAATACTCGCCGGTTCCTGGGACTGAAGGCCAGAAATATCAACGGGAAGAAA
ATGATTTACCTTGTTGTC
Protein sequence predicted based on nucleotide sequence
MWPGPALLLLGLGLGLGSQPPPTGPRGLPGVLRGAPGLGQGAESSVRGGDTGGLSPRAAP RHASPTPPRRCPSGAAARVLLKVNSSDPAAAKANVSCQTAPCIMQPVKINRKDQNAPLIL SRDEEATLNITVRWYCPDALVIRKSWVYFSVASVNDTPDWNRPVLLPQVAVSKGFSLHIP KYALPYGVYVFNFTLSIFRWDLAWPTVTGSDIIYIWVRKRPLKAVLLGAPRVTVKFSDEL ILNGSMSYDPEADIPTWGLQFFWYCTTNPRYYSGNYVLVINQAVCHPEQDSLNWPWAVGS VLTIPPKTLRGNGVYYFRMVVQKQNRTAFSDKTVHVLQGLQPKAHISCIENCGPTLGVSD RFSLFLNCPSCGRQDLYSWSILSLTGHEVMFDWAGQAITRRNGPYLSIKAFAFRNFLENR VWVSLILKSWSGMTTTLRHPVVINHGPRVGKCKINPASGISMVTKFAVECSDFKDENLPL KYKIIVSELDSIGAISSVEENTLGSVVYSGAEPITPAFFLPVGTVTDEYNVRLYVQVYDS LGTFSQVTLQATVTAPTVRDSSKDVLQQLLNVTMKPTSLLSTLLQKQDWLQAGYLTYVVI SVLNNIKGEQELQDDRARLLEHLVKQSLTFAMNTADEIGQSVMVITKLTQKASDLSQADL EATSFRLRQASQDLQEYQHRHKHLQQVEVVGTGILTSLSNLLKLINPYYTLQDPLFVVES LSDTILANKVPGSKTTALRTSNFNMYVEKVENWNVFKAFRNDSLCPNCLRATLNASTVPR LPAKAPISMMFCEFADDPFPWLTYPENISVDVVGFRMTGVADNNKVIEITPDIAEVYLVR KDLTPSSFNLTVGPGIKSDGFSKVTTGGISFEVDSRWTGELLIHIVTNVTVLFEALVYEG CQLTPTNLMATFLVPNDIPPIVKWSGLFDPTCSVKEARVVCLSSSLLRSIAQRRFSFKYN ISMVLQASRFVLKPTNKLVRIALFMVHCLDMYGIQSDWQQSTCVLGEKTTWKTVHCVCRN GRRSRRQLTSVKLTYHHLHTHFVTAKVIVVPNPVDLRLAIISNLTQNPATFLAVLFIMIL YAILAFWALHRDVIDLYFRDNVVILTDNDPFDTLCYLVTIFTGSRWGSGTRANVFIQLMG TEGTSDVHCLSHPYFKTLYRGSINTFLLTTKNDLGDIHSIRVWHDNSGEAPSWYLSRIKV ENLFNKRIWLFVCRRWLSVDTTLDATFSVTNPDEPLKRTDFFMIDVRDKLRKNHMWISIF TEIVPKPFNRLQRLSCCLAMLLSSLVCNIMFFNLNQKEKIESRHMHIIRSMLIGIESVVI TIPVQLLITFFFTYSQKNLKMNLDKVAPQKHPLMSEEGLSWKERLHKWHEYEMKALPRRA AVSTSAPEEKEAFETSQKHEKADTQMSNKNSSNNNQEASEGVPPKAFSSQPHTTESVQKK TQIILPRWCVYIAWFLVFATSGISSFFIVFYGVTYGYAKSIEWLFASFCSFCQSVFLVQP CNILLRSGTRSYKPKYCKNLSWSSKYHYSEIRLQGLTMTQEEMEQLHEDIAYVRSLSMYQ PITEDKIQILRRENRIRRRSFLFLSYLVTHFIFLTLLLLLIFSLRHNDSFYYNQFIRHRF SVDLATVMKLGDIYTWLHGVFLPLLHNDPNPTFLPDSSSKILGLPLVRQVRARPSNKTCL LAKKFVQSSVAGEIHCHPQYGIDPEDTQHYSSVWSKAGKQSTDKASHGFTYKPPGKRWVY HSYGVLNTYGSGGYVFYFFPGQQMFNSTVRLKELEGKNWLDELTWAVIVELTTLNPDTSL MCSISVVFEVSPLGVVNSSLSVYSFSLADFNRKTSSEIYLYAAILIFFCAYVVDEGYIIR QERASYIRSVYNLLNFSLKCMFALLIVLFFWKYFLATKMVQLYLADPEAFIPFHAVSRVD HFMRIILAFLLFLTILKTLRYSRFFYNVRLAQKAIQAALPGICHTALVVSIYSFMYVAFG YLVFGQHEWNYSNMIHATQTIFSYCVSAFQNTEFSGNKVLGVLFLSSFMLVMICIFINLF QAVILSAYDEMKQPVYEEPSDEAEAVTYLCNRLKSGFDFLTTRSRDKDQSNFFVDMLYGQ PEKNTRRFLGLKARNINGKKMIYLVV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
6381
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
