Gene:
Gene Symbol:
Med13
Gene Name:
mediator complex subunit 13
Original Clone ID:
FLH270311.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083237
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6513nts
Open reading frame: 1 to 6513
ATGAGTTCCTCCTTCGTGTCGAACGGGGCCAGCCTGGAAGATTGTCACTGTAACCTCTTC
TGCCTGGCCGACTTGACAGGAATTAAGTGGAAAAGATATGTATGGCAAGGCCCAACTTCC
GCCCCTATTCTGTTTCCGGTGACAGAAGAAGACCCCATTTTGAGCAGTTTTAGTCGATGC
CTTAAGGCAGATGTACTTGGTGTTTGGCGGCGAGATCAAAGACCTGGAAGAAGAGAATTG
TGGATATTTTGGTGGGGTAAAGATCCCAATTTTGCTGACCTTATTCACCATGACTTATCA
GAAGAGGAAGATGGAGTATGGGAGAATGGCCTTTCCTATGAATGCCGTACTTTGCTTTTC
AAAGCAGTTCACAATCTGTTGGAGCGATGTTTGATGAACCGAAACTTTGTACGAATTGGC
AAGTGGTTTGTGAAGCCATATGAGAAAGATGAAAAACCTATAAATAAAAGTGAACACTTG
TCCTGCTCTTTCACCTTTTTCTTGCATGGAGACAGCAATGTTTGTACCAGTGTGGAAATT
AACCAACATCAACCTGTATACCTTCTCAGTGAAGAGCATGTCACCCTTGCTCAACAGTCT
AATAGCCCATTTCAAGTTATCTTAAGTCCATTTGGACTAAATGGCACTCTCACGGGACAG
GCTTTCAAGATGTCTGATTCAGCTACGAAAAAATTAATTGGTGAATGGAAACAGTTCTAT
CCTATTTCATGTGGTTTGAAGGAGATGTCTGAGGAGAAACAGGACGATATGGATTGGGAA
GACGATTCTTTGGCTGCGGTGGAAGTTCTTGTTGCGGGTGTCCGAATGATCTACCCAGCA
TGCTTTGTGCTAGTCCCTCAGTCAGACATTCCTGCTCCTAGCTCTGTGGGAGCTTCTCAC
TGTTCAGCTTCTTGCTTGGGTATCCATCAAGTGCCTGCTTCCACAAGAGATCCTGCTATG
TCTTCAGTTACTCTCACTCCACCTACATCTCCTGAGGAAGTCCAAACAGTTGATCCTCAG
TCCGCACAGAAGTGGGTCAAATTTTCTTCAGTATCCGATGGCTTCAGCACTGACAGTACT
AGCCATCATGGTGGGAAAATACCCAGAAAATTAGCAAATCATGTGGTGGATAGAGTCTGG
CAAGAATGTAATATGAACAGATTACAGAACAAGAGGAAGTATTCTGCTACATCCAGCGGC
CTGTGTGAGGAAGAGACTGCCGATAAAATAGGATGCTGGGACTTTGTTGAAGCCACACAA
AGAACAAGCTGCAGTTGTTTGAGGCATAAAAGTCTCAAGACAAGAAATACTGGGCAACAA
GGACAGGCACCATCTTTAGGGCAGCAACAGCAAGTACTTCCTAAGCACAAGACCAATGAG
AAGCAAGACAAGAGTGAGAAGCCACAGAAGCGCCCCTTGACTCCCTTTCACCATCGTGTA
TCAGTTAGTGATGAGATTGGCATGGACACAGATTCAGCCAGCCAAAGACTTGTGATCTCT
GCTGCAGATAGTCAAGTGAGGTTTTCAAACATCCGAACTAATGATGTAGCAAAGACTCCT
CAGATGCATGGCACTGAACTGGCAAATTCACCTCAGCCTCCCCCACTTAGTCCTCATCCT
TGTGATGTGGTTGATGAAGGAGTGACTAAAACACCTTCAACTCCGCAGAGTCAACATTTT
TATCAAATGCCAACACCGGATCCCTTGGTTCCTACCAAACCAATGGAAGATAGGATAGAC
AGTTTGTCCCAGTCTTTCCCACCTCCATTTCAGGAAGCTGTAGAACCTACCGTGTATGTT
GGTACAGCAGTAAGCTTGGAAGAAGATGAAGCTAATGTAGCCTGGAAGTATTACAAAGTC
CCAAAGAAAAAAGATGTAGAATTTTTACCACCTCAACTTCCAAATGATAAATTCAAGGAT
GATCCAGTTGGACCTTTTGGACAGGAAAGTGTAACATCAGTTACAGAGTTAATGGTGCAG
TGTAAGAAGCCTTTAAAAGTTTCTGATGAAATAGTCCAGCAATATCAAATTAAAAATCAG
TATCTTTCAGCAATAGCATCTGATACAGAACAAGAACCTAAAATTGATCCATATGCATTT
GTTGAAGGAGATGAGGAATTCATTTTTACTGATAAAAAAGATAGACAAAATAGCGAGAGA
GAAGCTGGGAAGAAACACAAGGTGGAAGATGGGACATCTGCTGTCACAGTGTTATCACAC
GAAGAAGATGCTATGTCATTATTTAGTCCCTCTAAACAAGATGCTCCACGCCCTACAAAT
CATGCCCGTCCTCCATCAACCAGTTTGATTTATGACTCAGATTTGGCTGTGTCTTACACT
GACCTTGATAACCTCTTCAATTCTGATGAAGATGAATTAACACCTGGATCTAAAAAGTCA
GCAAGTGGATCAGATGATAAAGCCAGCTCCAAAGAATCAAAGACAGGAAATCTGGATCCG
TTGTCCTGCATAAGCACTGCAGACCTCCACAAAATGTACCCCACGCCACCCTCACTGGAA
CAGCACATTATGGGCTTCTCTCCAATGAATATGAATAACAAAGAATACGGTAGTGTGGAC
ACAGCACCCGGAGGGACTGTCCTGGAAGGAAATAGCTCTAGTGTAGGAACGCAGTTCAGA
ATCGAGGTCGAGGAAGGATTCTGTAGCCCTAAACCGTCTGAAATTAAAGATTTTTCTTAT
GTCTATAAGCCCGAAAATTGTCAAGTTCTAGTAGGATGTTCCATGTTTGCACCTTTAAAA
ACTCTACCAAGCCATTGTCTGCCTCCTATCAAATTGCCAGAAGAGTGTGTTTACCGGCAG
AGCTGGACTGTTGGAAAATTGGACTTGCTTCCTTCAGGCCCTTCAATGCCATTCATCAAG
GAGGGTGATGGCAGTAATTTGGATCAGGACTATGGCCCCGCCTACACACCGCAAACCCAT
GCTTCTTTTGGGATGCCTCCGAGCAGCGCACCTCCTAGTAATGGTGGTGCAGGAATTCTT
CCTTCCCCATCCACCCCTAGGTTTCCAACTCCAAGGACTCCAAGGACTCCAAGGACCCCT
CGTGGGGCTGGTGGGCCTGCTAGTGCTCAAGGTTCAGTCAAGTATGAGAATTCAGACTTG
TATTCACCAGCTTCCACCCCATCTACATGCCGACCCCTCAATTCTGTTGAACCTGCAACT
GTTCCTTCCATCCCTGAAGCGCACAGTCTTTATGTAAACCTCATCCTTTCAGAATCAGTA
ATGAATTTGTTTAAAGACTGTAATTTTGATAGTTGCTGTATTTGTGTCTGCAATATGAAT
ATCAAGGGTGCCGATGTTGGAGTTTACATTCCAGACCCAACCCAAGAAGCACAGTATCGG
TGCACCTGTGGCTTCAGTGCTGTCATGAACCGAAAATTTGGAAATAATTCAGGATTATTT
CTTGAAGATGAACTTGACATCATAGGACGTAACACAGACTGTGGGAAAGAAGCAGAAAAA
CGTTTTGAAGCTCTCAGGGCTTCCTCTGTGGAGAATGTTAATGGAGGCCTGAAGGAATCT
GAAAAGGTGCCTGATGAATTAATATTACTGCTACAAGATCAATGCACTAATTTATTTTCA
CCCTTTGGAGCAGCAGACCAAGATCCTTTTCCCAAAGTGGGTATAAGTAGCAATTGGGTG
CGTGTTGAGGAGCGGGACTGTTGTAGTGACTGCTGCCTCGCCTTAGAACATGGGCGTCAG
TTCATGGATAATATGTCAGGAGGGAAAGTTGATGAAGCACTTGTCAGAAGCTCATGCTTA
CATCCGTGGGCCAAGCAAAATGATGCGAGCGTGCAGTGTTCACAGGATATACTTCGGATG
CTCCTTTCTCTTCAGCCAGTTCTTCAAGATGCTATTCAGAAAAAAAGAACAGTGAGGCCT
TGGGGTGTTCAAGGTCCTCTCACATGGCAACAATTTCATAAAATGGCTGGCCGAGGCTCT
TATGGAACTGATGAATCTCCGGAACCACTGCCGATTCCCACGTTTTTGTTGGGTTATGAT
TACGATTTTCTGGTGCTTTCTCCATTTGCTCTCCCTTACTGGGAGAAACTTATGCTGGAA
CCCTATGGATCTCAAAGAGATATAGCCTATGTTGTACTCTGTCCAGAGAATGAAGCTTTG
TTAAATGGAGCCAGAAGCTTTTTTAGAGATCTCACTGCAATATATGAGTCCTGTCGATTA
GGCCAACACAGACCTATTTCTCGATTGTTGACGGATGGGATCATGAAAGTGGGAGCTACT
GCATCAAAGAAACTTTCAGAAAAGTTTGTAACAGAATGGTTTTCTCAGGCAGCTGATGGT
AACAATGAAGCATTTTCCAAATTGAAGCTGTATGCACAAGTCTGCAGATATGATTTAGGT
CCATATCTTGCTTCTCAGCCGTTGGACAGCTCTCTGCTCTCCCAGCCAAATTTAGTTGCC
CCTCCAAATCAGTCTTTGGTTACTGCACCTCAGATGACAAATACTGGAAACGCTAATGCT
CCATCTGCCACCTTAGCATCTGCGGCAAGTAGCACTATGACAATGACTTCAGGTGTTCCC
ATATCTACTTCAGTTGCCACAGCTAATTCAACTTTGACCACAACTTCATCTTCATCATCT
TCCAGTCTGAGTAGTGGAGTATCATCAAATAAACTGCCTTCGTTTCCACCCTTTGGCAGT
ATGAACACTAGTGGTACAGGATCCATGTCTGCACAAGCAAGTACGGTTCAGAGTGGCCAG
CTAGGAGGACAGCAGTCGTCATCTCTGCAGGCAGCAGGAATATCAGGGGAGTCAGCTTCT
CTTCCCACTCAGCCACATCCTGATGTGTCTGAAAGCACAATGGATCGTGATAAAGTGGGG
ATTCCCACAGATGGTGATTCACATGCAATTACCTACCCTCCTGCAATTGTTGTTTATATA
ATCGATCCTTTTACATATGAAAATAAAGACGAGAGCACTAACTCTTCTAATGTATGGACT
TTGGGGCTCCTTCGATGCTTCTTGGAAATGGTCCAAACTCTTCCTCCTCATATCAAGAGC
ACTGTTTCTGTGCAGATTGTTCCTTGTCAGTACCTGTTACAACCTGTGAAACACGACGAC
AGACAGATCTACTCCCAGCATTTAAAATCTTTGGCTTTTTCGGTGTTTACCCAGTGTCGG
AGACCACTTCCAACATCAACCAATGTAAAAACACTGACTGGGTTTGGTCCAGGCTTAGCT
ATGGAAACTGCTCTTAAAAGTCCTGATAGACCAGAGTGTATTCGACTTTATACACCACCT
TTTATTCTGGCTCCGGTGAAGGACAAACAAACAGAGCTAGGAGAGACCTTTGGAGAAGCT
GGACAGAAATACAATGTTCTTTTTGTGGGCTACTGTTTATCACATGATCAAAGGTGGATT
CTTGCATCTTGCACAGATCTATATGGAGAACTCCTAGAAACATGTATCATTAACATCGAT
GTTCCAAATAGGGCCCGTCGGAAAAAGGGTTCTGCTAGAAGATTTGGTCTACAGAAACTG
TGGGAGTGGTGCTTGGGACTTGTTCAGATGAGCTCTTTACCATGGAGAGTTGTGATTGGT
CGCCTGGGAAGGATTGGACATGGAGAGTTGAAAGATTGGAGTTGTTTGCTGAGTCGTCGA
AACTTGCAATCTCTAAGCAAAAGGCTCAAAGACATGTGTAGGATGTGTGGCATATCTGCT
GCAGACTCTCCAAGTATCCTTAGTGCTTGTTTGGTTGCAATGGAACCCCAAGGTTCTTTT
GTTATCATGCCAGATTCTGTGTCAACTGGTTCTGTTTTTGGAAGAAGTACCACTCTAAAT
ATGCAGACGCCTCAGCTAAATACCCCACAAGATACATCCTGTACTCATATACTTGTGTTC
CCTACTTCTGCTTCTGTGCAAGTAGCTTCAGCTACTTACACCACTGAAAATTTGGATTTA
GCTTTCAATCCCAACAATGATGGAGCAGACGGAATGGGTATCTTTGATTTATTAGACACA
GGAGATGATCTTGATCCTGATATCATTAATATCCTTCCTGCATCTCCAACTGCATCTCCT
GTACATTCTCCAGGCTCTCATTACCCCCATGGAGGTGATGCTGGCAAGGGTCAGGGTACT
GATCGGCTACTTTCAACAGAATCGCATGATGAAGTAACTAATATTCTTCAACAACCATTG
GCCCTTGGATACTTTGTATCAACTGCCAAAGCGGGTCCATTACCTGACTGGTTCTGGTCA
GCATGCCCTCAAGCACAATATCAATGCCCCCTTTTTCTTAAGGCCTCTTTGCACCTCCAC
GTGCCTTCAGTGCAATCTGACGAGCTGCTTCACAGCAAACACTCCCACCCACTGGACTCA
AATCAGACTTCAGATGTCCTCAGGTTTGTTTTGGAACAGTACAATGCACTCTCATGGCTA
ACCTGTGACCCTGCAGTCCAGGACAGACGCTCATGTCTCCCAGTTCATTTTGTGGTGCTG
AATCAGTTATATAACTTCATCATGAATATGCTG
Protein sequence predicted based on nucleotide sequence
MSSSFVSNGASLEDCHCNLFCLADLTGIKWKRYVWQGPTSAPILFPVTEEDPILSSFSRC LKADVLGVWRRDQRPGRRELWIFWWGKDPNFADLIHHDLSEEEDGVWENGLSYECRTLLF KAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEI NQHQPVYLLSEEHVTLAQQSNSPFQVILSPFGLNGTLTGQAFKMSDSATKKLIGEWKQFY PISCGLKEMSEEKQDDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPAPSSVGASH CSASCLGIHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSAQKWVKFSSVSDGFSTDST SHHGGKIPRKLANHVVDRVWQECNMNRLQNKRKYSATSSGLCEEETADKIGCWDFVEATQ RTSCSCLRHKSLKTRNTGQQGQAPSLGQQQQVLPKHKTNEKQDKSEKPQKRPLTPFHHRV SVSDEIGMDTDSASQRLVISAADSQVRFSNIRTNDVAKTPQMHGTELANSPQPPPLSPHP CDVVDEGVTKTPSTPQSQHFYQMPTPDPLVPTKPMEDRIDSLSQSFPPPFQEAVEPTVYV GTAVSLEEDEANVAWKYYKVPKKKDVEFLPPQLPNDKFKDDPVGPFGQESVTSVTELMVQ CKKPLKVSDEIVQQYQIKNQYLSAIASDTEQEPKIDPYAFVEGDEEFIFTDKKDRQNSER EAGKKHKVEDGTSAVTVLSHEEDAMSLFSPSKQDAPRPTNHARPPSTSLIYDSDLAVSYT DLDNLFNSDEDELTPGSKKSASGSDDKASSKESKTGNLDPLSCISTADLHKMYPTPPSLE QHIMGFSPMNMNNKEYGSVDTAPGGTVLEGNSSSVGTQFRIEVEEGFCSPKPSEIKDFSY VYKPENCQVLVGCSMFAPLKTLPSHCLPPIKLPEECVYRQSWTVGKLDLLPSGPSMPFIK EGDGSNLDQDYGPAYTPQTHASFGMPPSSAPPSNGGAGILPSPSTPRFPTPRTPRTPRTP RGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESV MNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLF LEDELDIIGRNTDCGKEAEKRFEALRASSVENVNGGLKESEKVPDELILLLQDQCTNLFS PFGAADQDPFPKVGISSNWVRVEERDCCSDCCLALEHGRQFMDNMSGGKVDEALVRSSCL HPWAKQNDASVQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGS YGTDESPEPLPIPTFLLGYDYDFLVLSPFALPYWEKLMLEPYGSQRDIAYVVLCPENEAL LNGARSFFRDLTAIYESCRLGQHRPISRLLTDGIMKVGATASKKLSEKFVTEWFSQAADG NNEAFSKLKLYAQVCRYDLGPYLASQPLDSSLLSQPNLVAPPNQSLVTAPQMTNTGNANA PSATLASAASSTMTMTSGVPISTSVATANSTLTTTSSSSSSSLSSGVSSNKLPSFPPFGS MNTSGTGSMSAQASTVQSGQLGGQQSSSLQAAGISGESASLPTQPHPDVSESTMDRDKVG IPTDGDSHAITYPPAIVVYIIDPFTYENKDESTNSSNVWTLGLLRCFLEMVQTLPPHIKS TVSVQIVPCQYLLQPVKHDDRQIYSQHLKSLAFSVFTQCRRPLPTSTNVKTLTGFGPGLA METALKSPDRPECIRLYTPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWI LASCTDLYGELLETCIINIDVPNRARRKKGSARRFGLQKLWEWCLGLVQMSSLPWRVVIG RLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSF VIMPDSVSTGSVFGRSTTLNMQTPQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDL AFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTASPVHSPGSHYPHGGDAGKGQGT DRLLSTESHDEVTNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLH VPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPAVQDRRSCLPVHFVVL NQLYNFIMNML
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
6516
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
