DNASU Plasmid | Amigo3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Amigo3

Gene Name: adhesion molecule with Ig like domain 3

Original Clone ID:

FLH270319.01L

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00083245

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1524nts

Open reading frame: 1 to 1524

ATGGCCTGGCTAGTGCTATCAGGTATACTACTATGCATGTTGGGTGCTGGATTGGGCACT
TCAGACTTGGAGGATGTTCTGCCTCCTGCTCCCCACAACTGCCCCGATATATGCATCTGT
GCTGCCGATGTGTTGAGCTGTGCGGGCCGTGGGTTACAGGACTTGCCGGTAGCACTGCCT
ACCACTGCTGCAGAACTCGATTTGAGCCACAACGCACTCAAACGCCTGCACCCGGGGTGG
TTAGCGCCCCTCTCCCGGCTGCGTGCCTTGCACCTAGGCTATAATAAGCTGGAAGTCCTG
GGCCATGGTGCGTTCACCAATGCCAGTGGCCTGAGGACACTTGACCTGTCCTCTAATATG
TTAAGGATGCTCCATACCCATGACCTGGATGGCCTGGAGGAGCTGGAGAAGTTACTTCTG
TTCAATAACAGCCTGATGCACTTGGACCTGGATGCCTTCCAGGGCCTGCGCATGCTTAGC
CACCTCTATCTCAGCTGCAACGAGCTCTCCTCTTTCTCTTTCAACCACTTGCACGGTCTG
GGGTTAACCCGCCTGCGGACTCTGGACCTCTCCTCCAACTGGCTGAAACATATCTCCATC
CCTGAGTTGGCTGCACTGCCAACTTATCTCAAGAACAGGCTCTACCTGCACAACAACCCG
CTGCCCTGTGACTGCAGCCTCTACCACCTGCTCCGGCGCTGGCACCAGCGGGGCCTGAGT
GCCCTGCATGATTTTGAACGCGAGTACACATGCTTGGTCTTTAAGGTGTCAGAGTCCCGA
GTGCGCTTTTTTGAGCACAGCCGGGTCTTCAAGAACTGCTCTGTGGCTGCAGCTCCAGGC
TTAGAGCTGCCTGAAGAGCAGCTGCACGCGCAGGTGGGCCAGTCCCTGAGGCTCTTCTGC
AACACCAGTGTGCCTGCCACTCGGGTGGCCTGGGTCTCCCCGAAGAATGAGCTGCTTGTG
GCGCCAGCCTCTCAGGATGGTAGCATCGCTGTGTTGGCTGATGGCAGCTTAGCCATAGGC
AGGGTGCAAGAGCAGCACGCAGGCGTCTTTGTGTGCCTGGCCAGTGGGCCCCGCCTGCAC
CACAACCAGACACTTGAGTACAATGTGAGTGTGCAAAAGGCTCGCCCCGAGCCAGAGACT
TTCAACACAGGCTTTACCACCCTGCTGGGCTGTATTGTGGGCCTGGTGCTGGTGTTGCTC
TACTTGTTTGCACCACCCTGTCGTGGCTGCTGTCACTGCTGTCAGCGGGCCTGCCGCAAC
CGTTGCTGGCCCCGGGCATCCAGTCCACTCCAGGAGCTGAGCGCACAGTCCTCCATGCTT
AGCACTACGCCACCAGATGCACCCAGCCGCAAGGCCAGTGTCCACAAGCATGTGGTCTTC
CTGGAGCCGGGCAAGAAGGGCCTCAATGGCCGTGTGCAGCTCGCAGTAGCTGAAGACTTC
GATCTGTGCAACCCCATGGGCTTGCAACTCAAGGCTGGCTCTGAATCAGCCAGTTCCACG
GGCTCAGAGGGTCTCGTGATGAGC

Protein sequence predicted based on nucleotide sequence

MAWLVLSGILLCMLGAGLGTSDLEDVLPPAPHNCPDICICAADVLSCAGRGLQDLPVALP
TTAAELDLSHNALKRLHPGWLAPLSRLRALHLGYNKLEVLGHGAFTNASGLRTLDLSSNM
LRMLHTHDLDGLEELEKLLLFNNSLMHLDLDAFQGLRMLSHLYLSCNELSSFSFNHLHGL
GLTRLRTLDLSSNWLKHISIPELAALPTYLKNRLYLHNNPLPCDCSLYHLLRRWHQRGLS
ALHDFEREYTCLVFKVSESRVRFFEHSRVFKNCSVAAAPGLELPEEQLHAQVGQSLRLFC
NTSVPATRVAWVSPKNELLVAPASQDGSIAVLADGSLAIGRVQEQHAGVFVCLASGPRLH
HNQTLEYNVSVQKARPEPETFNTGFTTLLGCIVGLVLVLLYLFAPPCRGCCHCCQRACRN
RCWPRASSPLQELSAQSSMLSTTPPDAPSRKASVHKHVVFLEPGKKGLNGRVQLAVAEDF
DLCNPMGLQLKAGSESASSTGSEGLVMS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1748

Start on reference sequence 222

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Amigo3

Symbol Nomenclature :

Amigo3

SYNONYM :

E430002N15Rik; ali3; mKIAA1851

Designation :

AMIGO-3|alivin 3|alivin-3|amphoterin induced gene and ORF 3|amphoterin-induced protein 3

Full Nomenclature :

adhesion molecule with Ig like domain 3

GENEID :

320844

GI :

162319099

GenBank Accession :

BC156236

Mouse Genome Informatics :

2444854

Vega :

OTTMUSG00000016524

Target GenBank:

NM_177275

Protein

SMART domain :

IGc2 : Immunoglobulin C-2 Type

SMART domain :

IG : Immunoglobulin

SMART domain :

LRRNT : Leucine rich repeat N-terminal domain

SMART domain :

LRR_TYP : Leucine-rich repeats, typical (most populated) subfamily

UniProt :

Q8C2S7

Clone

Cloning Information :

270319

HIP Master Clone ID :

201821

IMAGE ID :

100061628

Original Clone ID :

FLH270319.01L

Organism

Species Specific ID:

320844

Genome

Chromosome :

Map Location :

9 F2|9

Ensembl :

ENSMUSG00000032593

Reagents

Labome :

Amigo3-antibody