Gene:
Gene Symbol:
Met
Gene Name:
met proto-oncogene
Original Clone ID:
FLH270328.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083254
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4137nts
Open reading frame: 1 to 4137
ATGAAGGCTCCCACCGTGCTGGCACCTGGCATTCTGGTGCTGCTGTTGTCCTTGGTGCAG
AGGAGCCATGGGGAGTGCAAGGAGGCCCTAGTGAAGTCTGAGATGAACGTGAACATGAAG
TATCAGCTCCCCAACTTCACGGCAGAAACCCCCATCCAGAATGTCGTCCTACACGGCCAT
CATATTTATCTCGGAGCCACAAACTACATTTATGTTTTAAATGACAAAGACCTTCAGAAG
GTATCCGAATTCAAGACCGGGCCCGTGTTGGAACACCCAGATTGTTTACCTTGTCGGGAC
TGCAGCAGCAAAGCCAATTCATCAGGAGGGGTTTGGAAAGACAACATCAACATGGCTCTG
CTTGTTGACACATACTATGATGATCAACTCATTAGCTGTGGCAGTGTCAACAGAGGGACT
TGCCAGCGGCATGTCCTTCCTCCTGACAATTCTGCTGACATCCAGTCTGAGGTCCACTGC
ATGTTCTCCCCAGAAGAGGAGTCAGGGCAGTGTCCTGACTGTGTAGTGAGTGCCCTCGGA
GCCAAAGTCCTCCTGTCGGAAAAGGACCGGTTCATCAATTTCTTTGTGGGGAATACGATC
AATTCCTCCTATCCTCCTGGTTATTCACTGCATTCGATATCGGTGAGACGGCTGAAGGAA
ACCCAAGATGGTTTTAAGTTTTTGACAGACCAGTCCTATATTGATGTCTTACCAGAATTC
CAAGATTCCTACCCCATAAAGTACATACATGCCTTCGAAAGCAACCATTTTATTTACTTT
CTGACTGTCCAAAAGGAAACTCTAGATGCTCAGACTTTTCATACAAGAATAATCAGGTTC
TGTTCCGTAGACTCTGGGTTGCACTCCTACATGGAAATGCCCCTGGAATGCATCCTGACA
GAAAAAAGAAGGAAGAGATCCACAAGGGAAGAAGTGTTTAATATCCTCCAAGCCGCGTAT
GTCAGTAAACCAGGGGCCAATCTTGCTAAGCAAATAGGAGCTAGCCCTTCTGATGACATT
CTCTTCGGGGTGTTTGCACAAAGCAAGCCAGATTCTGCTGAACCTGTGAATCGATCAGCA
GTCTGTGCATTCCCCATCAAATATGTCAATGACTTCTTCAACAAGATTGTCAACAAAAAC
AACGTGAGATGTCTCCAGCATTTTTACGGACCCAACCATGAGCACTGTTTCAATAGGACC
CTGCTGAGAAACTCTTCCGGCTGTGAAGCGCGCAGTGACGAGTATCGGACAGAGTTTACC
ACGGCTTTGCAGCGCGTCGACTTATTCATGGGCCGGCTTAACCAAGTGCTCCTGACATCC
ATCTCCACCTTCATCAAAGGTGACCTCACCATTGCTAATCTAGGGACGTCAGAAGGTCGC
TTCATGCAGGTGGTGCTCTCTCGAACAGCACACCTCACTCCTCATGTGAACTTCCTCCTG
GACTCCCATCCTGTATCTCCAGAAGTTATTGTTGAGCATCCATCAAATCAAAATGGCTAT
ACATTGGTTGTCACAGGAAAGAAGATCACCAAGATTCCATTGAATGGCCTGGGCTGTGGA
CATTTCCAATCCTGCAGTCAGTGCCTCTCTGCCCCTTACTTTATACAGTGTGGCTGGTGC
CACAATCAATGTGTGCGTTTTGATGAATGCCCCAGCGGTACATGGACTCAAGAGATCTGT
CTGCCGGCGGTTTATAAGGTGTTCCCCACCAGCGCGCCCCTTGAAGGAGGAACAGTGTTG
ACCATATGTGGCTGGGACTTTGGATTCAGGAAGAATAATAAATTTGATTTAAGGAAAACC
AAAGTTCTGCTTGGCAACGAGAGCTGTACCTTGACCTTAAGCGAGAGCACGACAAATACG
TTGAAATGCACAGTTGGTCCCGCGATGAGTGAGCACTTCAATGTGTCTGTAATTATCTCA
AACAGTCGAGAGACGACGCAATACAGTGCATTCTCCTATGTAGATCCTGTAATAACAAGC
ATTTCTCCGAGGTACGGCCCTCAGGCTGGAGGCACCTTACTCACTCTTACTGGGAAATAC
CTCAACAGTGGCAATTCTAGACACATTTCAATTGGAGGGAAAACATGTACTTTAAAAAGT
GTATCAGATAGTATTCTTGAATGCTACACCCCAGCCCAAACTACCTCTGATGAGTTTCCT
GTGAAATTGAAGATTGACTTGGCTAACCGAGAGACCAGCAGCTTCAGTTACCGGGAAGAC
CCCGTTGTCTATGAAATCCACCCGACCAAATCTTTTATTAGTGGTGGAAGCACAATAACG
GGTATTGGGAAGACCCTGAACTCGGTTAGCCTCCCAAAGCTGGTAATAGATGTGCATGAA
GTGGGTGTGAACTACACAGTGGCATGTCAGCATCGCTCAAATTCAGAGATCATCTGCTGC
ACTACTCCTTCACTGAAACAGCTGGGCCTGCAACTCCCCCTGAAGACCAAAGCCTTCTTC
CTGTTAGACGGGATTCTTTCCAAACACTTTGATCTCACTTATGTGCATAATCCTGTGTTT
GAGCCTTTTGAAAAGCCAGTAATGATCTCAATAGGCAATGAAAATGTAGTGGAAATTAAG
GGAAACAATATTGACCCTGAAGCAGTTAAAGGTGAAGTGTTAAAAGTTGGAAATCAGAGC
TGCGAGAGTCTCCACTGGCACTCTGGAGCTGTGTTGTGTACAGTCCCCAGTGACCTGCTC
AAACTGAACAGCGAGCTAAATATAGAGTGGAAGCAAGCAGTCTCTTCAACTGTTCTTGGA
AAAGTGATCGTTCAACCGGATCAGAATTTTGCAGGATTGATCATTGGTGCGGTCTCAATA
TCAGTAGTAGTTTTGTTATTATCCGGGCTCTTCCTGTGGATGAGAAAGAGAAAGCATAAA
GATCTGGGCAGTGAATTAGTTCGCTATGACGCAAGAGTACACACTCCTCATTTGGATAGG
CTTGTAAGTGCCCGAAGTGTAAGTCCAACTACAGAGATGGTTTCAAATGAGTCTGTAGAC
TACAGAGCTACTTTTCCAGAAGACCAGTTTCCCAACTCCTCTCAGAATGGAGCATGCAGA
CAAGTGCAATACCCTCTGACAGACCTGTCCCCTATCCTGACAAGTGGAGACTCTGATATA
TCCAGCCCATTACTACAAAATACTGTTCACATTGACCTCAGTGCTCTAAATCCAGAGCTG
GTCCAAGCAGTTCAGCACGTAGTGATTGGACCCAGCAGCCTGATTGTGCATTTCAATGAA
GTCATAGGAAGAGGGCATTTTGGCTGTGTCTATCATGGGACTTTGCTGGACAATGACGGA
AAGAAAATTCACTGTGCTGTGAAATCCTTAAATAGAATCACAGATATAGAAGAGGTCTCC
CAGTTTCTGACTGAGGGAATCATCATGAAAGACTTCAGCCATCCCAATGTTCTCTCACTC
TTGGGAATCTGCCTGAGGAGTGAAGGGTCTCCTCTGGTGGTCCTGCCCTATATGAAGCAT
GGAGATCTGCGAAATTTCATTCGAAACGAGACTCATAATCCAACTGTGAAAGATCTTATA
GGATTTGGCCTTCAAGTAGCCAAAGGCATGAAATATCTTGCCAGCAAAAAGTTTGTCCAC
AGAGACTTAGCTGCAAGAAACTGCATGTTGGATGAAAAATTCACTGTCAAGGTTGCTGAT
TTCGGTCTTGCCAGAGACATGTACGATAAAGAGTACTATAGTGTCCACAACAAGACGGGT
GCCAAGCTACCAGTAAAGTGGATGGCTTTAGAGAGTCTGCAAACGCAGAAGTTCACCACC
AAGTCAGATGTGTGGTCCTTTGGTGTGCTCCTCTGGGAGCTCATGACGAGAGGAGCCCCT
CCTTATCCCGACGTGAACACATTTGATATCACTATCTACCTGTTGCAAGGCAGAAGACTC
TTGCAACCAGAATACTGTCCAGACGCCTTGTACGAAGTGATGCTAAAATGCTGGCACCCC
AAAGCGGAAATGCGCCCGTCCTTTTCCGAACTGGTCTCCAGGATATCCTCAATCTTCTCC
ACGTTCATTGGGGAACACTACGTCCACGTGAACGCTACTTATGTGAATGTAAAATGTGTT
GCTCCATATCCTTCTCTGTTGCCATCCCAAGACAACATTGATGGCGAGGGGAACACA
Protein sequence predicted based on nucleotide sequence
MKAPTVLAPGILVLLLSLVQRSHGECKEALVKSEMNVNMKYQLPNFTAETPIQNVVLHGH HIYLGATNYIYVLNDKDLQKVSEFKTGPVLEHPDCLPCRDCSSKANSSGGVWKDNINMAL LVDTYYDDQLISCGSVNRGTCQRHVLPPDNSADIQSEVHCMFSPEEESGQCPDCVVSALG AKVLLSEKDRFINFFVGNTINSSYPPGYSLHSISVRRLKETQDGFKFLTDQSYIDVLPEF QDSYPIKYIHAFESNHFIYFLTVQKETLDAQTFHTRIIRFCSVDSGLHSYMEMPLECILT EKRRKRSTREEVFNILQAAYVSKPGANLAKQIGASPSDDILFGVFAQSKPDSAEPVNRSA VCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARSDEYRTEFT TALQRVDLFMGRLNQVLLTSISTFIKGDLTIANLGTSEGRFMQVVLSRTAHLTPHVNFLL DSHPVSPEVIVEHPSNQNGYTLVVTGKKITKIPLNGLGCGHFQSCSQCLSAPYFIQCGWC HNQCVRFDECPSGTWTQEICLPAVYKVFPTSAPLEGGTVLTICGWDFGFRKNNKFDLRKT KVLLGNESCTLTLSESTTNTLKCTVGPAMSEHFNVSVIISNSRETTQYSAFSYVDPVITS ISPRYGPQAGGTLLTLTGKYLNSGNSRHISIGGKTCTLKSVSDSILECYTPAQTTSDEFP VKLKIDLANRETSSFSYREDPVVYEIHPTKSFISGGSTITGIGKTLNSVSLPKLVIDVHE VGVNYTVACQHRSNSEIICCTTPSLKQLGLQLPLKTKAFFLLDGILSKHFDLTYVHNPVF EPFEKPVMISIGNENVVEIKGNNIDPEAVKGEVLKVGNQSCESLHWHSGAVLCTVPSDLL KLNSELNIEWKQAVSSTVLGKVIVQPDQNFAGLIIGAVSISVVVLLLSGLFLWMRKRKHK DLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGACR QVQYPLTDLSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNE VIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIEEVSQFLTEGIIMKDFSHPNVLSL LGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLASKKFVH RDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTT KSDVWSFGVLLWELMTRGAPPYPDVNTFDITIYLLQGRRLLQPEYCPDALYEVMLKCWHP KAEMRPSFSELVSRISSIFSTFIGEHYVHVNATYVNVKCVAPYPSLLPSQDNIDGEGNT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
356
End on reference sequence
4495
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
