Gene:
Gene Symbol:
Cntnap5c
Gene Name:
contactin associated protein-like 5C
Original Clone ID:
FLH270330.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083256
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3915nts
Open reading frame: 1 to 3915
ATGGATTCTGTACCAAGACTGAATAGCGTTTTTACTTTGGTGCTCTCTGGCTTGTGGCAT
TTTGGATTAACAGCGACAAATTACAACTGTGATGACCCATTGACTTCCTTCCTCTCACTG
AGGGCTTTCTCCAGCTCCTCAGATCTCACTGGAAGGTCCAGCCCAGCTCAACTCAACTGG
AGAATGGGAACTGGTGGTTGGTCCCCAGCAGATTCTAATGCCCAGCAGTGGCTCCAAATG
GACCTGGGAAACAGAGTGGAGATTACAGCTGTGGCTACCCAGGGAAGATATGGGAGCTCT
GACTGGGTGACAAGCTATCGCCTGATGTTCAGTGACACAGGGCACAACTGGCAACAGTAC
ACGCAGGAAGGCAGCATCTGGAGATTTGTAGGAAACATGAATGCTAACAGTGTGGTTCAC
CACAAACTGCTGAACTCTGTGAGAGCCCGCTTTGTTCGCTTTGTGCCCCTGGAGTGGAAT
CCAAATGGAAAGATTGGCATGAGAGTAGAGGTCTATGGATGTTCCTACAGATCAGATGTA
GCTGACTTTGATGGCTGGAGCTCACTTCTCTACAGGTTCAACCAGAAGACAATGAGCACC
CTCAAAGATGTGATCTCCCTTAAGTTCAAGAGCATACAAAGGGATGGGGTTCTGTTCCAT
GGGGAAGGACAACGTGGAGACCATATCACCCTAGAGCTTCAGAATGGCAGGCTAGCCCTA
TACCTGAACCTGGATGACAGCAAAGCCCAGGTCAGCAGCACTGCACCCTTGGCAACTCTG
GGTAGCCTTCTGGATGACCAGCACTGGCACTCAGTTCTCCTGGAGCGTGTGGGCAAGCAG
GCCAACTTCACTGTGGACAAGAACACACAGCACTTCCAGACCAAGGGTGAGACTGATGCC
CTGGACATTGACTATGAGCTCAGTTTTGGAGGAATTCCAGTACCAAGCAAACCTGGGACC
TTTTTAAAGAAAAACTTCCATGGCTGTATAGAAAACCTTTATTACAATGGAGTGAACATA
ATTGACTTGGCTAAAAGACGGAAGCATCAGATTTACTCAGGCAATGTCACCTTTTCCTGC
TCAGAACCACAAACTGTCCCCATCACATTTGTCAACTCCAGGAGCAGTTATTTGCTGTTG
ACTGGTACCCCCCAAATTGATGGACTGTCAGTGAGTTTCCAGTTTCGAACATGGAATGAG
GATGGTCTGCTTCTGTCCACAGAACTGTCTGAAGGCTCAGGAACCCTGCTGCTCATCCTG
GAGGGCGGGACTCTGAGGCTCCTGATTAAGAAATTGGCCAGACATGGAACTGAAATCTTC
ACAGGCAGTGGCTTGAATGATGGCATGTGGCATTCTGTCAGCATCAGTGCCAGAAGAAAT
CGTGTTACTCTCACACTGGATAATGATGCTGCATCCCTGCCTCCAGACACCTCCTGGCTG
CAGATATATTCTGGAAATAGCTACTACTTTGGAGGGTGCCCTGACAATCTCACTGATTCC
CAATGCTTAAACCCGATTAAGGCTTTCCAAGGCTGCATGAGGCTCATCTTTATTGATAAC
CAGCCCAAGGATCTTATTTCAGTTCAGCAAGGCTCCCTGGGGAGTTTTAGTGATTTACAC
ATTGATCTGTGTAGCATCAAAGACAGGTGTTTGCCAAACTACTGTGAACATGGAGGACAA
TGTGCGCAAACCTGGACTAACTTCTACTGCAACTGCAGTGACACTGGTTACACAGGAGCC
ACCTGTCATGACTCCATCTATGAGCAGTCCTGTGAGGTGTACAGACACAGAGGGAAAACT
GCTGAGTTCTTCTATGTTGACTCTGATGGCAGCGGGCCACTGGGACCACTCCAGGTGTTC
TGCAATATTACTGAAGACAAGATCTGGATGACAGTTCAGCACAACAACACAGGGCTGACT
TGGGTACAGGGTTCTAACCCTGAGAAGCCCTATGCCATGACCTTAAACTATGGTGGGAGT
CTGGAGCAACTGGAGGCTCTGATTGATGGCTCAGAGCACTGTGAACAGGAAGTGACATAC
TACTGCAAGAGATCCCGCCTGCTTAACACACCAGATGGAGTCCCATTCACCTGGTGGATT
GGGAGGTCCAATGAAAAGCATCCTTATTGGGGAGGCTCTCTTCCTGGAGTCCAGCAGTGT
GGATGTGGGCTGGAAGAGAGCTGCCTGGACATTCGACATTTTTGCAATTGTGATGCTGAC
ACAGATGAATGGACAAACGATACTGGTTATCTGTCTTTCAAAGATCACTTGCCCGTAACT
CAGATAATTATTACTGATACCAACAGATCAAAATCAGAAGCTGCTTGGAGAATTGGCCCC
TTGCGTTGCTATGGTGACCGACACTTCTGGAATGCTGTCTCATTTTCTACCGAAGCCTCT
TTCCTTCACTTTCCTACCTTCCGTGTGGAATTCAGCGCTGACATTTTCTTCTTTTTTAAA
ACCACTGCTTTATCAGGAGTTTTCCTAGAAATCCTTGGCATTAAAGACTTCCTCCGACTT
GAAATGAGCTCTCCTTCTGAGGTCATATTTGCCATTGATGTTGGAAATGGTCCCATTGAT
CTCCTCGTCCAGTCTCCTTATCCTCTGAATGACAACCAATGGCATTATATCCGGGCTGAG
AGAAACCTCAAGGAGACCTCATTGCAGGTGGACAATCTTCCACAGAGTATGAGGGAGGCC
TCAGAGGAAGGCCATTTCCAATTTCAGCTGAATAGCCAGTTGTTTGTAGGAGGAAAATCA
TCAAGACAAAAAGGCTTTTTTGGATGCATCCGTTCCTTACACTTGAATGGGCAGAACATA
GACCTGGAGGAGAGAGCAAAGGTCACATCTGGAGTGAGACCTGGATGTCCAGGTCACTGC
AGCAGTTATGGAAGAAATTGCCAAAATGGGGGCAAGTGTGTGGAGAAGCATATTGGCTAC
TCATGTGACTGTACCAACTCACCATATGAAGGACCTTTCTGTCAGAAAGAGATTTCAGCT
CTTTTTGATTCTGACACCTCTGTTACATACATGTTTCAAGAACCATACTCAGTGACCAAG
AACACAAACCTCTCATCTTCTGCTATCTACACAGATACAGCTCCATCCAAGGAAATCATC
ATGCTGAGTTTCATGACAGCACAAGCCCCCACCCTCTTGCTCTACCTCAATTTCTCTTCT
CAGAATTTTCTGGCTATTTTGCTCTCCTGGAATGGAAGTTTACAGATTCACTATCAACTA
AGCAAGGAAGAAAGCCATGTGTTCACCATCAACACAGAGAATCTGGCCAATAGAAGAGTG
CACCAGGTGAAAATGAGCCGTGATGGTCCAGAACTTTCTATTCAGATGGACCAGCAACTC
TTCAGCTATACTTTCTCACTAGAATCTGAGTTCCAGAGAGCAAGGTCGCTCGTCCTAGGC
AAAGTCACAGAGACTCTTGGGTTGGATCCAGAAGTTGCTAGAGCAAACACCTTGGGTTTT
GTTGGATGTCTTTCTTCAGTCCAATACAATCATATAACACCATTGAAGGCAGCCCTTCGC
CATGCCAGCATTTCACCTGTGACTGTTCAAAGGACTTTGACAGAATCCAGCTGTGTCTCC
ATGGTGGACTCTGATGCGAATGCAGTGACCACCGTGTATTCTTCAACAGATCCATTTGGA
GAGAGAGATGAGCGTGAACCGCTCACAAATGCAGTCCCAAGTGACTTGGCAGTCATTGGA
GGCATCATAGCAGTGGTGACATTCATCAGTTTTTCTGTCATTGGAATCATGACCCACTTT
TTCTATCAGCACAAGCGGTCACACTATGCCAGTCAAATGAAGGAGAAGGAATACCCAGAG
AATGTGGACAGTTCCTCTAGAAATGACATTGACTTGCAAAACACAACCAGGGAGTGCAAG
CAGGAAGACTTCATA
Protein sequence predicted based on nucleotide sequence
MDSVPRLNSVFTLVLSGLWHFGLTATNYNCDDPLTSFLSLRAFSSSSDLTGRSSPAQLNW RMGTGGWSPADSNAQQWLQMDLGNRVEITAVATQGRYGSSDWVTSYRLMFSDTGHNWQQY TQEGSIWRFVGNMNANSVVHHKLLNSVRARFVRFVPLEWNPNGKIGMRVEVYGCSYRSDV ADFDGWSSLLYRFNQKTMSTLKDVISLKFKSIQRDGVLFHGEGQRGDHITLELQNGRLAL YLNLDDSKAQVSSTAPLATLGSLLDDQHWHSVLLERVGKQANFTVDKNTQHFQTKGETDA LDIDYELSFGGIPVPSKPGTFLKKNFHGCIENLYYNGVNIIDLAKRRKHQIYSGNVTFSC SEPQTVPITFVNSRSSYLLLTGTPQIDGLSVSFQFRTWNEDGLLLSTELSEGSGTLLLIL EGGTLRLLIKKLARHGTEIFTGSGLNDGMWHSVSISARRNRVTLTLDNDAASLPPDTSWL QIYSGNSYYFGGCPDNLTDSQCLNPIKAFQGCMRLIFIDNQPKDLISVQQGSLGSFSDLH IDLCSIKDRCLPNYCEHGGQCAQTWTNFYCNCSDTGYTGATCHDSIYEQSCEVYRHRGKT AEFFYVDSDGSGPLGPLQVFCNITEDKIWMTVQHNNTGLTWVQGSNPEKPYAMTLNYGGS LEQLEALIDGSEHCEQEVTYYCKRSRLLNTPDGVPFTWWIGRSNEKHPYWGGSLPGVQQC GCGLEESCLDIRHFCNCDADTDEWTNDTGYLSFKDHLPVTQIIITDTNRSKSEAAWRIGP LRCYGDRHFWNAVSFSTEASFLHFPTFRVEFSADIFFFFKTTALSGVFLEILGIKDFLRL EMSSPSEVIFAIDVGNGPIDLLVQSPYPLNDNQWHYIRAERNLKETSLQVDNLPQSMREA SEEGHFQFQLNSQLFVGGKSSRQKGFFGCIRSLHLNGQNIDLEERAKVTSGVRPGCPGHC SSYGRNCQNGGKCVEKHIGYSCDCTNSPYEGPFCQKEISALFDSDTSVTYMFQEPYSVTK NTNLSSSAIYTDTAPSKEIIMLSFMTAQAPTLLLYLNFSSQNFLAILLSWNGSLQIHYQL SKEESHVFTINTENLANRRVHQVKMSRDGPELSIQMDQQLFSYTFSLESEFQRARSLVLG KVTETLGLDPEVARANTLGFVGCLSSVQYNHITPLKAALRHASISPVTVQRTLTESSCVS MVDSDANAVTTVYSSTDPFGERDEREPLTNAVPSDLAVIGGIIAVVTFISFSVIGIMTHF FYQHKRSHYASQMKEKEYPENVDSSSRNDIDLQNTTRECKQEDFI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3918
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
