Gene:
Gene Symbol:
Kcnh4
Gene Name:
potassium voltage-gated channel, subfamily H (eag-related), member 4
Original Clone ID:
FLH270336.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083262
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3054nts
Open reading frame: 1 to 3054
ATGCCGGTCATGAAGGGGTTGCTGGCCCCGCAAAACACCTTTCTGGACACCATCGCCACC
CGCTTTGACGGCACGCACAGCAACTTCCTTCTGGCCAATGCACAGGGCCCACGGGGTTTT
CCCATCGTCTACTGCTCTGACGGCTTCTGTGAGCTCACAGGCTACGGCCGCACCGAGGTC
ATGCAGAAAACCTGTAGCTGTCGGTTCCTCTATGGCCCAGAGACCAGTGAACCAGCCCTG
CAACGGTTGCAAAAAGCCCTGGAGGGCCACCAAGAACACCGAACTGAAATCTGCTTTTAC
CGAAAGGATGGCTCAGCCTTCTGGTGTCTTCTGGACATGATGCCCATCAAGAATGAGATG
GGGGAGGTTGTGCTTTTCCTATTTTCCTTCAAGGACATCTCTCAGAGTGGAGGCCCAGGA
CTTGGCTCACCAGGAATCCATGGGGACAATAATCACGAAAACTCTCTTGGGAGGAGAGGA
ACTAGCTCAAGACTTAGGTCCACGAAGAGGCAGAACCGGACTGTCCTACACCGGTTGACT
GGCCACTTTGGTCGCCGGGGCCAGAGCAGTGTGAAGGCCAACAGTAACCTGTTTGAGCCA
AAACCATCAGTGCCCGAGTACAAGGTGGCCTCTGTGGGGGGCTCCCGCTGCCTCCTCCTC
CACTACAGCATCCCCAAGGCTGTCTGGGATGGTCTCATCCTCCTCGCCACATTCTACGTC
GCGGTCACTGTCCCCTACAACGTCTGCTTCGCGGGTGATGACGACACCCCCATCACTTCC
CGACACACCCTTGTCAGTGACATCGCTGTGGAAATGCTCTTCATCCTGGACATCATCTTG
AACTTCCGCACCACCTACGTGTCCCAGTCTGGCCAGGTGATTTCGGCTCCTCGGTCCATT
GGCCTCCACTATCTGGCCACCTGGTTCTTTGTAGACCTCATTGCTGCTTTGCCCTTTGAT
CTGCTGTATGTCTTCAACATCACTGTGACCTCACTGGTACATCTGCTGAAAACCGTGCGG
CTCCTGCGGCTGCTGCGGCTGCTGCAGAAGTTAGAGCGGTACTCACAGTGCAGTGCAGTG
GTGCTCACGCTGCTCATGTCCGTCTTTGCCCTCCTTGCTCATTGGATGGCCTGCGTCTGG
TATGTCATCGGGCGCCGGGAGATGGAGGCCAATGATCCACTGCTCTGGGACATTGGTTGG
TTGCATGAGCTGGGCAAGCGGTTGGAGGAGCCTTATGTCAATGGCTCGGCAGGTGGACCA
TCTCGGCGCAGTGCCTACATCGCTGCGCTGTACTTCACGCTGAGCAGCCTCACCAGTGTA
GGCTTCGGCAACGTGTGTGCCAACACAGACGCTGAGAAGATCTTCTCCATCTGCACGATG
CTCATAGGCGCGCTGATGCACGCAGTGGTGTTTGGGAATGTCACAGCCATCATCCAGCGC
ATGTACTCCCGACGCTCGCTCTACCACAGCCGCATGAAGGATCTCAAGGACTTCATCCGA
GTGCATCGCCTGCCCCGCCCACTCAAGCAGCGCATGCTCGAGTACTTCCAGACGACGTGG
GCAGTCAACAGCGGCATCGATGCCAACGAGTTACTGCGTGACTTCCCGGATGAGCTGCGT
GCTGACATTGCTATGCACCTGAACAGGGAGATCCTGCAGCTGCCTCTGTTTGGAGCGGCT
AGCAGGGGCTGCCTGCGGGCCCTCTCCTTGCACATCAAGACCTCCTTCTGTGCTCCTGGG
GAGTACCTGTTACGCCGTGGGGATGCCCTCCAGGCACACTACTACGTCTGCTCTGGCTCG
CTTGAGGTGCTCCGGGACAACATGGTGCTGGCCATCCTGGGGAAGGGGGACTTGATTGGG
GCAGACATCCCTGAGTTGGGACAGGAGCCTGGGTCAGGGGCAGGCCCCAGCTGTGTGCTG
AAGACCAGCGCCGATGTGAAAGCTCTGACTTACTGTGGCCTGCAGCAGCTGAGCAGCCGA
GGGCTGGCGGAGGTCCTGCGGCTGTATCCGGAATATGCAGCTGCCTTCCGGGCTGGCCTA
CCCCGGGACCTAACCTTCAACCTGCGCCAAGGTTCTGAAAACAATGGCCTCGGCCGCTTC
TCACGGTCTCCTCGTCTCTCCCAGGCACGCTCCGACACTCTTGGTTCCTCCTCAGACAAG
ACTCTGCCATCCATCACAGAAACCGAGGGTGGCACGGAGCCTGGGGCTGGTTCCAAGCCC
CGTCGGCCCCACCTGCTGCCCAACCTCAGTCCAGCACGACCTCGGGGGTCCCTGGTCAGC
CTTTTGGGCGAGGAGCTGCCCCCATTCTCAGCCCTTGTCTCCTCTCCTTCCCTGTCCCCA
ACTCCTTCCCCTGCCCTGGCTGGCCGGGGTCAGAGTCCCTCCCCGCACGGCCCCCCCAGG
GGCTCTGCTGCCTGGAAGCCCCCCCAGCTCCTCATTCCCCCACTGGGAACATTTGGACCT
CCGGACCTCAGTCCCCGGATCGTGGATGGCATTGAAGACTCCAGTAACACAGCTGAGGCT
CCTACATTCCGCTTCAGCAAGAGGCCAGAGCCCACCAGAACTCGCTCACAGGCTCCCCCT
TCAGGCCCCAGGCTCAGCCGGGAACTGGCCACAGAGGCAGCAGAGGAGGTGAAGGAAAAG
GTCTGCAGGCTGAACCAAGAGATCTCCCGTCTCAATCAGGAAGTGTCTCAGCTGAGCCGG
GAGCTGCGGCAAGTGATGGGTCTCTTACAGGCCAGGCTGGGACCCCCAAGTCACACAGCT
GGTCCAGCTTGGCTCCCAGACCTTCCTTGCCCCCACCAGAGACTACCATGCATCTCTCCT
CATATGTCTGGACCACCACCTGGTCTCCAGAATACTACACTCGCTGTAGTCCACTGTCCA
GCCAGTGTTGGGACAGTGGAGATAGGGGCCACCCCCTCAGAGCTGAGACCTTCCACGATA
CCACCCTATCCCTCGGAACCTGATCCTCTGGGACCCTCTCCAGCGCCAGAGGCTCCTCCC
CTGACCCCAAGCCTCCTGAAGCACAGCTTCCAGTCTGGGTCAGACACATTCCAC
Protein sequence predicted based on nucleotide sequence
MPVMKGLLAPQNTFLDTIATRFDGTHSNFLLANAQGPRGFPIVYCSDGFCELTGYGRTEV MQKTCSCRFLYGPETSEPALQRLQKALEGHQEHRTEICFYRKDGSAFWCLLDMMPIKNEM GEVVLFLFSFKDISQSGGPGLGSPGIHGDNNHENSLGRRGTSSRLRSTKRQNRTVLHRLT GHFGRRGQSSVKANSNLFEPKPSVPEYKVASVGGSRCLLLHYSIPKAVWDGLILLATFYV AVTVPYNVCFAGDDDTPITSRHTLVSDIAVEMLFILDIILNFRTTYVSQSGQVISAPRSI GLHYLATWFFVDLIAALPFDLLYVFNITVTSLVHLLKTVRLLRLLRLLQKLERYSQCSAV VLTLLMSVFALLAHWMACVWYVIGRREMEANDPLLWDIGWLHELGKRLEEPYVNGSAGGP SRRSAYIAALYFTLSSLTSVGFGNVCANTDAEKIFSICTMLIGALMHAVVFGNVTAIIQR MYSRRSLYHSRMKDLKDFIRVHRLPRPLKQRMLEYFQTTWAVNSGIDANELLRDFPDELR ADIAMHLNREILQLPLFGAASRGCLRALSLHIKTSFCAPGEYLLRRGDALQAHYYVCSGS LEVLRDNMVLAILGKGDLIGADIPELGQEPGSGAGPSCVLKTSADVKALTYCGLQQLSSR GLAEVLRLYPEYAAAFRAGLPRDLTFNLRQGSENNGLGRFSRSPRLSQARSDTLGSSSDK TLPSITETEGGTEPGAGSKPRRPHLLPNLSPARPRGSLVSLLGEELPPFSALVSSPSLSP TPSPALAGRGQSPSPHGPPRGSAAWKPPQLLIPPLGTFGPPDLSPRIVDGIEDSSNTAEA PTFRFSKRPEPTRTRSQAPPSGPRLSRELATEAAEEVKEKVCRLNQEISRLNQEVSQLSR ELRQVMGLLQARLGPPSHTAGPAWLPDLPCPHQRLPCISPHMSGPPPGLQNTTLAVVHCP ASVGTVEIGATPSELRPSTIPPYPSEPDPLGPSPAPEAPPLTPSLLKHSFQSGSDTFH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3057
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
