Gene:
Gene Symbol:
Ern1
Gene Name:
endoplasmic reticulum (ER) to nucleus signalling 1
Original Clone ID:
FLH270339.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083265
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2931nts
Open reading frame: 1 to 2931
ATGCCGGCCCGGTGGCTGTTGCTCCTGCTGGCGCTGCTGCTACCGCCGCCCGGCCCCGGG
AGTTTTGGAAGAACCAGCACAGTTACACTGCCTGAGACCTTGTTGTTTGTCTCGACCCTG
GATGGAAGCTTGCATGCTGTTAGCAAGAGGACGGGCTCCATCAAGTGGACTTTAAAAGAA
GATCCAGTCCTGCAGGTCCCAACACACGTGGAAGAGCCGGCTTTCCTCCCAGATCCCAAT
GATGGCAGTCTGTACACACTTGGAGGCAAGAACAACGAAGGCCTGACGAAACTTCCCTTT
ACCATCCCAGAATTGGTTCAGGCCTCCCCATGCCGAAGTTCAGATGGAATCCTCTACATG
GGTAAAAAGCAAGATATTTGGTATGTTATCGACCTCCTGACTGGCGAGAAGCAGCAGACT
TTGTCATCGGCCTTTGCTGATAGTCTCTGCCCATCAACTTCCCTTCTATATCTTGGACGG
ACAGAATACACCATCACCATGTATGACACCAAGACCCGGGAGCTCCGCTGGAATGCCACC
TATTTTGACTATGCAGCCTCACTGCCGGAAGACGACGTGGACTACAAGATGTCCCACTTT
GTGTCCAATGGCGATGGACTGGTGGTAACTGTGGACAGTGAATCTGGGGATGTCCTGTGG
ATCCAAAACTATGCCTCTCCTGTGGTGGCCTTCTACGTCTGGCAGGGGGAGGTCCTGAGA
AAGGTGGTGCACATCAACGTTGCTGTGGAGACTCTACGCTACTTGACCTTCATGTCTGGG
GAAGTGGGGCGCATCACCAAGTGGAAGTATCCATTCCCCAAGGAGACAGAGGCCAAGAGC
AAGCTAACGCCTACTCTGTATGTTGGGAAGTATTCCACCAGCCTCTATGCCTCTCCCTCA
ATGGTGCATGAGGGGGTTGCTGTCGTGCCTCGAGGCAGCACTCTTCCTTTGCTGGAAGGC
CCCCAGACAGATGGCGTCACCATTGGAGACAAAGGAGAGTGTGTGATCACTCCCAGCACA
GACCTCAAGTTTGACCCTGGACTCAAAGGGAAGAGCAAGCTGAACTACTTGAGGAATTAC
TGGCTTCTCATAGGACACCATGAAACTCCTCTGTCTGCATCCACCAAGATGCTGGAGAGA
TTTCCTAACAACCTGCCCAAACATCGAGAAAATGTGATTCCTGCTGATTCAGAAAAAAGG
AGCTTTGAGGAAGTTATCAACATAGTTGGCCAGACTTCAGACAACACACCGACCACCGTA
TCTCAGGATGTGGAGGAGAAGCTCGCTCGCGCCCCTGCCAAGCCTGAGGCCCCCGTGGAC
TCCATGCTCAAGGACATGGCTACCATTATCCTGAGCACCTTCCTGCTGGTTGGATGGGTG
GCGTTCATCATCACTTACCCCCTGAGCGTGCATCAGCAGCGTCAGCTCCAGCACCAACAG
TTCCAGAAGGAGCTGGAGAAGATTCAGCTCCTGCAGCAGCAGCAGCTGCCCTTCCACCCA
CACGGAGACCTTACCCAGGACCCTGAGTTCCTGGATTCATCTGGCCCCTTCTCAGAGAGC
TCTGGCACCAGCAGCCCCAGCCCATCCCCCAGAGCCTCCAACCACTCCCTCCACCCCAGC
AGCTCTGCCTCCAGGGCCGGCACCAGCCCCTCTCTGGAGCAGGATGATGAGGATGAGGAA
ACCAGAATGGTGATTGTTGGGAAAATTTCATTCTGCCCCAAGGATGTCCTGGGTCATGGA
GCTGAGGGCACAATTGTATACAAAGGTATGTTTGACAACCGAGATGTGGCCGTGAAGAGG
ATCCTCCCTGAGTGTTTTAGCTTTGCCGACCGTGAGGTCCAGCTGCTTCGAGAATCAGAC
GAGCACCCAAATGTGATCCGCTACTTTTGCACAGAGAAGGACCGGCAGTTCCAGTACATT
GCTATCGAGCTGTGTGCAGCCACCCTACAAGAGTATGTGGAGCAGAAGGACTTTGCCCAC
CTTGGCCTCGAGCCCATCACCCTGCTTCATCAGACCACCTCAGGCCTGGCACACCTGCAT
TCTCTCAACATTGTTCACAGAGACCTGAAGCCCCACAACATTCTCCTCTCCATGCCCAAC
GCACATGGCAGGATCAAGGCGATGATCTCTGACTTTGGCCTCTGCAAGAAGCTGGCAGTG
GGCAGGCACAGTTTCAGCCGCCGTTCAGGGGTACCTGGCACTGAAGGGTGGATCGCCCCA
GAGATGCTGAGTGAAGACTGTAAGGACAACCCTACCTACACGGTGGACATCTTTTCTGCA
GGCTGTGTCTTTTACTATGTCATCTCTGAGGGCAACCATCCTTTTGGCAAATCCTTGCAG
CGGCAGGCCAACATCCTCCTGGGCGCCTGCAACCTTGACTGTTTCCACTCAGACAAGCAT
GAGGACGTCATTGCTCGTGAATTGATAGAGAAAATGATTGCTATGGATCCCCAGCAGCGT
CCCTCTGCAAAGCACGTGCTGAAACACCCCTTCTTCTGGAGCCTGGAGAAGCAGCTCCAG
TTTTTCCAGGATGTAAGTGACCGAATAGAAAAGGAGGCCTTGGACGGTCCAATCGTACGG
CAGTTGGAGAGAGGCGGGAGAGCTGTGGTCAAGATGGACTGGCGGGAGAACATCACTGTC
CCCCTGCAGACAGATCTGCGCAAATTCAGAACCTACAAAGGTGGCTCTGTGAGAGACCTC
CTCCGAGCCATGAGAAACAAGAAACACCACTACCGGGAGCTCCCCGTGGAGGTTCAGGAG
ACGCTGGGCTCCATCCCGGATGACTTTGTGCGCTACTTCACTTCCCGCTTCCCCCACCTC
CTCTCTCACACCTACCAAGCCATGGAGCTGTGCAGACATGAGAGACTCTTTCAGACCTAC
TACTGGCACGAGCCCACAGAACCCCAGCCTCCAGTGATTCCATATGCCCTC
Protein sequence predicted based on nucleotide sequence
MPARWLLLLLALLLPPPGPGSFGRTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKE DPVLQVPTHVEEPAFLPDPNDGSLYTLGGKNNEGLTKLPFTIPELVQASPCRSSDGILYM GKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNAT YFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQGEVLR KVVHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPS MVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDLKFDPGLKGKSKLNYLRNY WLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKRSFEEVINIVGQTSDNTPTTV SQDVEEKLARAPAKPEAPVDSMLKDMATIILSTFLLVGWVAFIITYPLSVHQQRQLQHQQ FQKELEKIQLLQQQQLPFHPHGDLTQDPEFLDSSGPFSESSGTSSPSPSPRASNHSLHPS SSASRAGTSPSLEQDDEDEETRMVIVGKISFCPKDVLGHGAEGTIVYKGMFDNRDVAVKR ILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAH LGLEPITLLHQTTSGLAHLHSLNIVHRDLKPHNILLSMPNAHGRIKAMISDFGLCKKLAV GRHSFSRRSGVPGTEGWIAPEMLSEDCKDNPTYTVDIFSAGCVFYYVISEGNHPFGKSLQ RQANILLGACNLDCFHSDKHEDVIARELIEKMIAMDPQQRPSAKHVLKHPFFWSLEKQLQ FFQDVSDRIEKEALDGPIVRQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDL LRAMRNKKHHYRELPVEVQETLGSIPDDFVRYFTSRFPHLLSHTYQAMELCRHERLFQTY YWHEPTEPQPPVIPYAL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
121
End on reference sequence
3054
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
