Gene:
Gene Symbol:
Prdm2
Gene Name:
PR domain containing 2, with ZNF domain
Original Clone ID:
FLH270340.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083266
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4983nts
Open reading frame: 1 to 4983
ATGCATCAGAACACTGAGTCTGTGGCAGTCACTGAGACTCTGGCTGAGGTACCTGAACAC
GTGCTTCGAGGACTTCCAGAGGAAGTAAGACTTTTACCATCTGCGGTCGACAAGACTCGG
ATTGGTGTCTGGGCCACTAAACCAATTTTAAAAGGGAAAAAGTTTGGGCCATTTGTTGGT
GATAAGAAGAAGAGATCCCAGGTGTACTACCCAAATTTGGGGTGGATGTGCATTGATGCC
ACTGATCCGGAGAAGGGCAACTGGCTCCGCTATGTGAACTGGGCTTGCTCAGGAGAAGAA
CAGAATTTATTTCCACTGGAAATCAACAGAGCCATTTACTATAAAACCTTAAAGCCAATC
GCGCCTGGCGAGGAGCTCCTGGTCTGGTACAATGGGGAAGACAACCCCGAGATAGCAGCT
GCGATTGAGGAAGAGCGAGCCAGCGCCCGGAGCAAGCGGAGCTCCCCGAAGAGCCGGAGA
GGGAAGAAGAAATCACAGGAGAATAAAAACAAAGGCGTCAGAACCCAGGCTGCAGCGCGG
AAGGCGAGCGAGCTGGACTCCACCTCTGCAAACATGAGGGGCTCTGCAGAAGGTCCAAAA
GAAGAGGATGAGAGGCCTTTGGCTTCTGCACCTGAGCAGCCAGCCCTTCTGCCAGAGGTG
GTAAGCCAGGATGCAGTTCCACAGGTGGCCATCCCTCTTCCTGCCTGCGAGTCACAGCCA
GGGGCAGATGGGAAACAAGAAGTCACAGACTGTGAGGTCAACAATATGAAGGAAGAGGAG
GAGGAGGAGGAGGAGGAGCTGGAAGAGGAAGAAGAGGAGGAGGAGTTGGGAGAAGATGGG
GAGGAAGAAGCAGACATGCCGAATGAAAGCTCTGTGAAAGAACCAGAAATACGGTGTGAA
GAAAAGCCAGAAGATTTACTGGAAGAGCCACGGAGTGTGCCAACTGAAACTTCTGAAGGC
TCCCCAGGCGCCACCCCTCCTCCCCACGCTCCCAGGGCTAGAGAGGAGGCCAACGGCGAG
GGGCTTGAAACATTCATGTTTCCATGTCAGCATTGTGAAAGGAAATTTGCAACGAAGCAG
GGGCTAGAACGTCACATGCACATCCACATTTCTACAATCAACCACGCTTTCAAGTGCAAG
TACTGTGGGAAACCATTTGGCACACAGATCAACAGGAGGCGGCATGAAAGGCGCCACGAA
ACAGGGTTGAAGAGAAGACCCAGCATGGCATTACAATCCTCAGAGGACCCAGACGATGGC
AAGGGGGAAAATGTTACTTCTAAAGATGAGTCGAGTCCACCTCAACTTGGGCAAGACTGT
TTGATATTGAACTCAGAGAAAACCTCACAGGAAATACTGAATTCATCTTTTGTGGAAGAG
AATGGTGAAGTTAAAGAACTTCACCCATGCAAATACTGCAAAAAGGTATTTGGAACTCAC
ACCAATATGAGACGACATCAGCGTAGAGTTCACGAACGCCACCTGATTCCCAAAGGTGTC
CGGCGAAAAGGAGGCCTCCTAGAAGAGCCGCAGCCACCTGCAGAGCAGGCCCCGCCCTCC
CAGAATGTCTATGTACCAAGCACAGAGCCCGAGGAGGACGGGGACGCAGATGATGTGTAT
ATCATGGACATTTCTAGCAACATCTCTGAGAACCTAAATTACTATATTGATGGTAAGATT
CAGACCAACAACAGCACTAGTGATTGTGATGTGATTGAGATGGAGTCTAACTCTGCACAC
TTGTATGGCATAGACTGTCTGCTCACTCCAGTAACTGTAGAGATTACTCAAAACATAAAG
AGCACTCAGGGCTCTGTGACAGATGATCTTCTTAAAGAGTCTCCCAGTAGCACAAATTGC
GAGTCTAAGAAACGGAGGACTGCCAGTCCACCTGTACTCCCTAAAATTAAAGCAGAGACA
GATTCTGATTCCACGGCACCCTCGTGTTCCTTAAGTCTGCCTCTGAGCATATCAACAACA
GAGGTGGTGTCTTTCCATAAAGAGAAGGGTGTCTATTTGTCTTCAAAGCTCAAGCAGCTT
CTTCAGACCCAGGACAAACTGACTCCTCCTGCAGGGATTTCAACAGCTGAGATTCCTAAG
TTAGGTCCCGTGTGTGTATCTGCTCCTGCATCCATGTTAGCTGTGACTTCTAGTAGGTTT
AAGAGACGCACCAGCTCTCCCCCCAGTTCTCCACAGCACAGCCCTGCCCTTCGAGACTTT
GGGAAACAGAGTGATGGGAAAGCAGCATGGACAGACACAGCTCTGACTTCCAAGAAACCG
AAGTTAGAAAGTCGTAGTGACTCACCAGCGTGGAGTTTGTCTGGGAGAGATGAGAGAGAA
ACTGGAAGCCCTCCTTGTTTTGATGAATACAAAATATCAAAAGAATGGGCAGCCAGTTCT
ACTTTCAGCAGCGTGTGCAACCAACAGCCGTTGGATTTATCCAGCGGTGTCAAACAGAAG
TCAGAGGGCACAGGCAAGACTCCAGTCCCATGGGAATCTGTATTGGATCTCAGTGTGCAT
AAAAAGCCTTGTGATTCTGAAGGCAAGGAATTCAAAGAGAACCATTTGGCACAGCCGGCT
GCAAAGAAGAAAAAACCAACCACCTGCATGCTACAAAAGGTTCTTCTCAATGAATATAAT
GGTGTTAGCTTACCTACAGAAAGCACACCAGAGGCGACCAGGAGCCCAAGTCCTTGTAAA
TCACCAGACACACAGCCAGATCCTGAACTTGCTACTGACTCGAGTTGCTCAGCCCCCACT
GCTGAGTCTCCACCTGAGGTTGTTGGCCCTTCATCACCCCCTCTCCAGGCAGCCTCCCTG
TCCTCTGGTCAGCTGCCTCCTCTCTTGATCCCCACAGAGCCTTCTTCCCCTCCGCCCTGC
CCTCCTGTGTTAACTGTTGCCACTCCACCACCTCCCCTCCTTCCGACTGTCCCTCTCCCC
AACCCCTCTTGTGATGCCTCTCCTCAACAGTGCCCCTCTCCATTCTCAAATGCCACTGCT
CAGTCTCCTCTTCCGATTCTCTCCCCAACAGTGTCCCCCTCTCCCTCTCCTATTCCTCCT
GTAGAGCCACTCATGTCTGCTGCTTCCCCTGGTCCTCCGACACTTTCCTCCTCTTCCTCC
TCCTCTTCCTCCTCTTCCTTCTCTTCCTCTTCATGTTCCTCCTCCTCCCCCTCACCACCC
CCTCTCTCAGCAGTGTCATCTGTGGTGTCCTCTGGGGACAACCTGGAAGCATCTCTCCCT
GCAATAACTCTCAAACAGGAGGAGTCAGAGAGTGAAGGTCTGAAACGCAAGGAAGAGGCC
CCAGCTGCAGGTGGGCAGAGCGCTGTCCAAGAGACATTCAGCAGAAACTTCGTCTGCAAT
GTGTGCGCATCACCTTTTCTTTCCATTAAAGACCTAACCAAACATTTGTCTGTCCATGCT
GAAGAATGGCCCTTCAAATGTGAGTTTTGTGTGCAGCTTTTTAAGGGTAAGACCGATCTA
TCAGAGCACCGATTTCTGCTTCATGGAGTCGGAAATATCTTTGTGTGTTCTGTATGTAAG
AAAGAATTTGCCTTCTTATGCAATTTACAGCAGCATCAGCGTGATCTCCACCCAGATGAG
TTATGCACACACCACGAGTTTGAAAGTGGGACCCTGAGGCCCCAGAACTTCACAGACCCC
AGCAAGGCCCATGTGGAGCATATGCCGAGTTTGCCAGAAGAGCCTGCGGAAGCTTCTAGA
GAGGAGGAGTTAAATGACTCCTCTGAAGAGCTTTACACGACCATCAAAATAATGGCTTCC
GGAATAAAGACGAAAGAGCCAGATGTTCGACTTGGTCTCAACCAGCATTACCCGAGCTTT
AAGCCACCTCCATTTCAGTACCACCATCGAAACCCTATGGGGATTGGGGTAACAGCCACA
AACTTCACCACCCACAATATCCCACAGACTTTCACTACTGCCATCCGCTGCACAAAGTGT
GGCAAGAGTGTTGATAACATGCCTGAGCTGCATAAGCACATCTTGGCGTGTGCATCTGCA
AGTGACAAGAAGAGGTACACTCCTAAGAAAAACCCAGTGCCGCTGAGACAGACGGTGCAG
CCCAAAAATGGAGTGCTGGTTTTAGACAACTCTGGGAAAAATGCCTTCAGACGGATGGGG
CAGCCTAAGAGACTGAGCTTCAATGTTGAACTCAGCAAAATGTCTCCAAATAAGCTCAAG
CTAAATGCATTGAAGAAAAAAAATCAGCTGGTACAGAAAGCGATCCTTCAGAAGAACAGA
TCTGCGAAGCAGAAGGCAGACCTGAGGGACACTTCCGAGGCGTCCTCACATATCTGCCCT
TACTGTGACAGGGAGTTCACGTACATCGGCAGCCTGAATAAGCATGCTGCTTTCAGCTGT
CCTAAAAAACCCCTTTCTCCTTCCAAAAGAAAAGTTTCTCATTCATCTAAGAAAGGTGGC
CACACATCATCTGCCAGCAGTGACAGAAACAGTAGTAGCAATCCCCGGAGGCGCACCGCA
GATACGGAGATCAAGATGCAGAGCATGCAGGCACCCTTGGGCAAGACCCGAGCTCGGAGT
ACAGGCCCTGCCCAAGCCTCGCTGCCCTCCTCATCCTTCAGATCCAGACAGAATGTCAAG
TTTGCAGCTTCAGTCAAGTCCAAAAAAGCAAGCTCTTCATCCTTGAGGAATTCTAGTCCC
GTAAGAATGGCTAAGATTACTCATGTTGAGGGCAAAAAATCCAAAGCTGTTGCCAAGAGT
CATTCTACTCAGCTCTCAAGCAAAGCATCCCGAAGCCTGCACGTGAAGGTGCAGAAGAGC
AAAGCTGTTCTACAAAGCAAGACTGCCCTGGCCAGCAAGAAAAGAACAGACCGGTTCATA
GTAAAATCTAGAGAGCGGAGCGGGGGCCCAATCACCCGAAGCCTTCAGCTGGCAGCTGCT
GCGGACCTGAGTGAAAGCAGGAGAGAGGACAGCAGTGCCAGGCATGAGCTGAAGGACTTG
AGG
Protein sequence predicted based on nucleotide sequence
MHQNTESVAVTETLAEVPEHVLRGLPEEVRLLPSAVDKTRIGVWATKPILKGKKFGPFVG DKKKRSQVYYPNLGWMCIDATDPEKGNWLRYVNWACSGEEQNLFPLEINRAIYYKTLKPI APGEELLVWYNGEDNPEIAAAIEEERASARSKRSSPKSRRGKKKSQENKNKGVRTQAAAR KASELDSTSANMRGSAEGPKEEDERPLASAPEQPALLPEVVSQDAVPQVAIPLPACESQP GADGKQEVTDCEVNNMKEEEEEEEEELEEEEEEEELGEDGEEEADMPNESSVKEPEIRCE EKPEDLLEEPRSVPTETSEGSPGATPPPHAPRAREEANGEGLETFMFPCQHCERKFATKQ GLERHMHIHISTINHAFKCKYCGKPFGTQINRRRHERRHETGLKRRPSMALQSSEDPDDG KGENVTSKDESSPPQLGQDCLILNSEKTSQEILNSSFVEENGEVKELHPCKYCKKVFGTH TNMRRHQRRVHERHLIPKGVRRKGGLLEEPQPPAEQAPPSQNVYVPSTEPEEDGDADDVY IMDISSNISENLNYYIDGKIQTNNSTSDCDVIEMESNSAHLYGIDCLLTPVTVEITQNIK STQGSVTDDLLKESPSSTNCESKKRRTASPPVLPKIKAETDSDSTAPSCSLSLPLSISTT EVVSFHKEKGVYLSSKLKQLLQTQDKLTPPAGISTAEIPKLGPVCVSAPASMLAVTSSRF KRRTSSPPSSPQHSPALRDFGKQSDGKAAWTDTALTSKKPKLESRSDSPAWSLSGRDERE TGSPPCFDEYKISKEWAASSTFSSVCNQQPLDLSSGVKQKSEGTGKTPVPWESVLDLSVH KKPCDSEGKEFKENHLAQPAAKKKKPTTCMLQKVLLNEYNGVSLPTESTPEATRSPSPCK SPDTQPDPELATDSSCSAPTAESPPEVVGPSSPPLQAASLSSGQLPPLLIPTEPSSPPPC PPVLTVATPPPPLLPTVPLPNPSCDASPQQCPSPFSNATAQSPLPILSPTVSPSPSPIPP VEPLMSAASPGPPTLSSSSSSSSSSSFSSSSCSSSSPSPPPLSAVSSVVSSGDNLEASLP AITLKQEESESEGLKRKEEAPAAGGQSAVQETFSRNFVCNVCASPFLSIKDLTKHLSVHA EEWPFKCEFCVQLFKGKTDLSEHRFLLHGVGNIFVCSVCKKEFAFLCNLQQHQRDLHPDE LCTHHEFESGTLRPQNFTDPSKAHVEHMPSLPEEPAEASREEELNDSSEELYTTIKIMAS GIKTKEPDVRLGLNQHYPSFKPPPFQYHHRNPMGIGVTATNFTTHNIPQTFTTAIRCTKC GKSVDNMPELHKHILACASASDKKRYTPKKNPVPLRQTVQPKNGVLVLDNSGKNAFRRMG QPKRLSFNVELSKMSPNKLKLNALKKKNQLVQKAILQKNRSAKQKADLRDTSEASSHICP YCDREFTYIGSLNKHAAFSCPKKPLSPSKRKVSHSSKKGGHTSSASSDRNSSSNPRRRTA DTEIKMQSMQAPLGKTRARSTGPAQASLPSSSFRSRQNVKFAASVKSKKASSSSLRNSSP VRMAKITHVEGKKSKAVAKSHSTQLSSKASRSLHVKVQKSKAVLQSKTALASKKRTDRFI VKSRERSGGPITRSLQLAAAADLSESRREDSSARHELKDLR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
161
End on reference sequence
5146
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
