DNASU Plasmid | Capn11 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Capn11

Gene Name: calpain 11

Original Clone ID:

FLH270367.01L

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00083292

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2142nts

Open reading frame: 1 to 2142

ATGCTGAACTACTCAGACATCTCTTGGGCTGAAAAAGGAATGGTGGCTACCATAAACCAC
TCCAGGCTCAAGGACAAGGGCGTGGGCCAACACCAAAATGCCTACAACTATAAGAATCAG
AACTATGAGGACCTTCGAGCAGAGTGTCTCAGGAAGGGGGAACTGTTCGAAGACCCCTTT
TTCCCTGCTGAACCCAGGTCTATCGGTGTCAAGAACCTGGGCCCCAACTCTGAACACATG
CAGAATATCTACTGGCAGCGGCCAAAGGATATCATTCATAACCCCCAGTTCATCACGAAT
GACTTCTCTCCCACGGACATCTGCCAAGGGATCCTGGGGGACTGCTGGCTCCTGGCTGCC
ATTGGCTCCCTTACCACCTGCCCCAAACTGCTGTACCGTGTGGTGCCCAGAAATCAGAGC
TTCAAGAAGAACTATGCTGGCATCTTCCACTTTCAGCTTTGGCAGTTTGGGCACTGGTTG
AACGTGGTCGTGGACGACCGGCTGCCCACTAGGAACAACAAGCTGGTATTCGTGCACGCC
TCCCATCGCCAGGATTTCTGGAGCGCCCTGCTGGAGAAGGCCTACGCCAAGCTGATCGGA
TCCTACGGAGCGCTGTCTGGAGGCAGCACTTTGGAGGGTTTGGAAGATTTCACAGGCGGC
GTAGCACAGTGCATTCCGCTCCAGAAGCCCCCTGGCAACATGCTAAGGCTCCTCAAGAAA
GCCCTGGAGAAGTCTTCCCTCATGGGCTGTTCCATTGAAGTCACAGACAATAGTGAAGTA
GAAACCATGACACATAACATGCTGGTGCGAGGACATGCTTATGCAGTGACTGGCCTAGAG
GATGTCTACTACAGAGACAAGTTGGAAACTCTAATTCGGATCCAAAATCCTTGGGGTCGG
GTTGAATGGAATGGAGCCTGGAGTGACAAAGCCACAGAGTGGGAAGAGGTGAGCCCAGAT
GTTCGGGTACAGCTGCTACACAAGAAGGATGATGGCGATTTCTGGATGTCCTATGAAGAC
TTCATGAGCAACTTTACACTGCTGGAGATCTGCAACCTCACCCCGGATGCCCTCAACACC
TGGGATTACAAGAGCCGCTGGCACTCCACCTTCTATGAGGGCAGCTGGCGAAGGGGAAGC
ACTGCAGGGGGCTGCAGAAACCACCCGGAGACGTTCTGGAGCAACCCCCAGTTCAAGATC
TCTCTCCCGGAAGTGGATGACCCAGAGGATGACTCTGAGAAAAACGAAATGGTTTGCACC
TGTCTGGTGGCACTGATGCAGAAGAACTGGAGACATGCTCGAGAGGGACCCCAGCTCCTC
ACCATAGGCTTTGTCATCTTCTCGGTCCCAAAGGAGTTTCAGAACCTCCGGGACATCCAC
CTTAAGAAAGACTTCTTCTTGAAATACCGGGACCACGGCTTTTCAGAGATCTTCATCAAC
TCGAGGGAGGTGAACAGCCATCTCCGGCTGCCCCCGGGGGAATACGTCATTATCCCCTCC
ACCTATGAGCCCCATAAAGATGCTGACTTCCTGCTTCGGGTCTTCACGGAGAAACACAGT
GAGACCTGGTTGCTGGATGACGCCAACCGGTTTGAACATCTCCAAGAGGAGACTGTCACT
GACAAGGATTTGGACAAGGATTCTCTTCAGCTGTTTAAGATAATGGCAAACGAGGATGGC
GAAGTGGATATGTATGCACTACACAAACTTCTCAACAGGATGACAGCTAAATTAAGGAAC
TTCAAGACGAAGGGCTTTAGCTTGGAAGTCTGTCGCCGGATGATCAACCTCCTGGATAAA
GATGGTTCTGGCAAGCTGGAGCTTCATGAATTCCAGGTCCTGTGGAAAAAGATCAAGAAA
TGGACGGAAATCTTCAAAGAGTGTGATGAGGACCGCTCAGGGAACTTGAACTCCTATGAG
ATGCGCCTGGCAATAGAGAAAGCAGGCATCAAGATGAACAACAGAGTGACCGAGGTAGTC
GTTGCCAGGTACTCGGATAACATGATTGTGGACTTTGACAGCTTCCTCAACTGTTTCCTG
AGGCTCAAGGCCATGTTTGCGTTCTTCTTATCCATGGACACCAAGAAAACTGGTTCTATT
TGCTTGGATATTAACCAGTGGCTGCAAATAACCATGTGGGGG

Protein sequence predicted based on nucleotide sequence

MLNYSDISWAEKGMVATINHSRLKDKGVGQHQNAYNYKNQNYEDLRAECLRKGELFEDPF
FPAEPRSIGVKNLGPNSEHMQNIYWQRPKDIIHNPQFITNDFSPTDICQGILGDCWLLAA
IGSLTTCPKLLYRVVPRNQSFKKNYAGIFHFQLWQFGHWLNVVVDDRLPTRNNKLVFVHA
SHRQDFWSALLEKAYAKLIGSYGALSGGSTLEGLEDFTGGVAQCIPLQKPPGNMLRLLKK
ALEKSSLMGCSIEVTDNSEVETMTHNMLVRGHAYAVTGLEDVYYRDKLETLIRIQNPWGR
VEWNGAWSDKATEWEEVSPDVRVQLLHKKDDGDFWMSYEDFMSNFTLLEICNLTPDALNT
WDYKSRWHSTFYEGSWRRGSTAGGCRNHPETFWSNPQFKISLPEVDDPEDDSEKNEMVCT
CLVALMQKNWRHAREGPQLLTIGFVIFSVPKEFQNLRDIHLKKDFFLKYRDHGFSEIFIN
SREVNSHLRLPPGEYVIIPSTYEPHKDADFLLRVFTEKHSETWLLDDANRFEHLQEETVT
DKDLDKDSLQLFKIMANEDGEVDMYALHKLLNRMTAKLRNFKTKGFSLEVCRRMINLLDK
DGSGKLELHEFQVLWKKIKKWTEIFKECDEDRSGNLNSYEMRLAIEKAGIKMNNRVTEVV
VARYSDNMIVDFDSFLNCFLRLKAMFAFFLSMDTKKTGSICLDINQWLQITMWG

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2194

Start on reference sequence 50

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Capn11

Symbol Nomenclature :

Capn11

Designation :

CANP 11|calcium-activated neutral proteinase 11|calcium-dependent thiol protease|calpain-11

Full Nomenclature :

calpain 11

GENEID :

268958

GI :

151555308

GenBank Accession :

BC148637

Mouse Genome Informatics :

1352490

Vega :

OTTMUSG00000031350

Target GenBank:

NM_001013767

Protein

SMART domain :

calpain_III :

SMART domain :

EFh : EF-hand, calcium binding motif

SMART domain :

CysPc : Calpain-like thiol protease family.

UniProt :

Q6J756

Clone

Cloning Information :

270367

HIP Master Clone ID :

201869

IMAGE ID :

100015642

Original Clone ID :

FLH270367.01L

Organism

Species Specific ID:

268958

Genome

Chromosome :

Map Location :

17 B3|17

Ensembl :

ENSMUSG00000058626

Reagents

Labome :

Capn11-antibody