Nlrp9a (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Nlrp9a
Gene Name:  NLR family, pyrin domain containing 9A
Original Clone ID:
FLH270415.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083340
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3012nts
Open reading frame: 1 to 3012

ATGATGGACTCATCTGGGTATGGCTTGCTGCAGTATTTGCAAAAGCTCAGTGATGAAGAG
TTCCAGAGATTTAAAGAACATCTCAGGAAAGAGCCAGAGAAATTTAAACTTAAACCTATC
TCCTGGACTAAGATCAAAAACACTTCCAAAGAAGACCTGGTAATGCAATTGTACACACAT
TATCCAGGAAAGGCATGGGATATGGTGTTGAGCCTGTTTCTACAGGTCAATCGGGAAGAC
CTCTCAACCATGGCTCAGACAGAAAGGAGAGATAAACAAACTAAATACAAAGAGTTTATG
AAGAATACATTTCAGCACATATGGACAATGGAGACCAACACTTATATACCTGATAGAAGC
TACCACGAATTTATAGAAGTCCAGTACAGAGCATTGCAGGACATATTTGATTGTGAGTCA
GAGCCAGTCACTGTAGTTGTATCAGGTTCTAGAGGAGGAGGTAAAACTACTTTCTTAAGA
AAAGCAATGTTGGATTGGGCATCAAGAAATTTACTGCAGAACCGATTTCAATATGTTTTC
TACTTCTCCGTCTTCTCACTTAACAATATCACAGAGTTAAGCTTAGCTGAGCTTATCTCA
AGTACGTTGCCTGAATCTTCAGAGACAGTGGATGATATTTTATCTGATCCAAAAAGAATC
TTGTTTATCCTGGATGGATTTGATTACCTGAAATTTGACTTGGAACTCAGGACAAACCTG
TGCAATGACTGGAGGAAGAAGCTGCCAATACAAATTGTCTTGAGTAGTTTGCTACAGAAA
ATAATGCTCCCAGAATGTTCTCTGCTCCTTGAATTGGGAAATGCAAGTCTTTCAAACATT
ATTCCTTTGCTGCAATATCCAAGGGAGATAATTATGTCAGGATTCTCTGAACAAACTATA
GAAATCTACTGTGTGTCTTTCTTTAATACCCAGACAGGTGTAGAAATCTTTAAGAATTTA
AAGAGCATTAAACCATTGTTCAATTTATGCCGCTGTCCTCATCTATGCTGGATGATCTGT
AGTACTATAAAGTGGCAGTATGAGAGGAGAGAGGTAGCAAGTCGTTTTGGTCGAACATTA
GGACTCCTCTACACAATATTTATGGTTAGCGCATTTAAATCAACATATGCCAGGAATCCA
TCTAAACAGAACAGGGCCCGTATAAGGACCCTATGCACCTTGGCTGTAGAGGGAATGTGG
AAACAGGTATATGTGTTTGACTCTGATGACCTCAGGAGGAATGGGATATCTGAATCAGAT
AAGAAAGTGTGGCTGAGAATGAAATTTCTCCAGAATCAAGGTAGCAACATTGTATTTTAT
CATTCTACACTACAGTGGTACTTTGCTGTGCTGTTCTATTTTCTCCAATACAAAGACACA
CGTCACCCAGTTATTGGAAACCTCGCTCAACTCCTAGGAGAAATCTATGCTCATAAACAG
AACCAATGGTTCCACACACGGATATTATTGTTTGGAATGGCCACTGAGCAAGTTAACTCC
TTGCTTGAACCATGCTTTGGCTGTATATCATCCAAGGAGGTAAGACAGGAAATCATCAGA
TACATCAAAAGTCTCAGTCAACAAGAATGCAATGAAAAACTGGTGGTGCATCCCCAGAAT
TTGTTTTTCTGTATACTTGACAACCAGGAGGAAAGATTTGTAAGACAATTGATGGATCGA
TTTGAAGAAATGACTGTTGATATTAGTGATGTTGATGATATGTCAGCAACTCCATATTGT
CTGCATAGAGCCCCAAAAGTGAAAAACCTTCACCTGCATATACAAAAGAGAGTTTTTTTA
GAAATCCATGATCCAGAATATGGTGACTTAGAACTCTTTAAACTCGACCAAAAACTTTTA
GCCAAGCATTGGACTACGTTATGCACTTTTCTCTGTAACTTACATGTGTTGGATCTGGAC
AGCTGTCATTTCAATGAAAAAGCCATAGAGGTTCTCTGTAATTGTTTGCCTCTAACTTCT
TTAGTACCTCTGACTGGTTTTAAGCTCCACAGATTGTTGTGTTCCTTCACGACTAACTTT
GGAGATGGTTTATTATTTTGCACATTTCTTCACCTACCTCACCTGAAATACATGAACCTG
TATGGCACTAATCTCTCTAATGATGCAGTTGAGAGGCTGTGCTCAGCTCTGAAATTTTCA
ACATGTGGAGTGGAGGAGCTGTTGTTGGGAAAATGTGACATATCAAGTGAGGCTTGTGGC
ATAATTGCAGCTTCCCTTATCAATTCTGAGGTGAAACATCTCTCATTGGTTGAAAATCCC
TTGAAGAACAAAGGAGTGATGTCACTGTGTGAAATGCTGAAGGATCCAAGCTGTGTCCTG
GAATCACTGATGTTGTCTTACTGCTGTCTCACATTCATTGCCTGTGGACATCTCTATGAA
GCTCTTTTGAGCAATGAACACCTCTCTCTGCTTGATCTGGGGTCAAATTTCCTGGAAGAC
ACTGGAGTGAACCTTCTGTGTGAAGCTTTGAAAGATCCAAACTGCACCCTCAAGGAGTTA
TGGTTGCCTGGTTGCTATTTAACTTCAGAATGTTGTGAAGAGATCTCGGCTGTCCTGACT
TGCAATACAAATCTAAAGACCCTGAAACTTGGCAACAATAACATACAAGACACTGGTGTC
AAACGGTTATGTGAAGCTCTGTGTCATCCTAACTGTGAAATGCAGTGTCTTGGGTTAGAC
ATGTGTGACTTCACCAGTGACTGTTGTGAAGACCTGGCCTTGGTTCTTACCACCTGCAAC
ACATTGAAGAGCTTAAACCTTGACTGGAATGCCTTTGACCATTCTGGGCTGGAGATGCTC
TGTAAGGCCTTGAATCACAAAGCCTGTAACCTGGAGGTGCTTGGACTGGACAAATCTTTA
TTTTCTGAGGAATCACAGACATTACTGCAAGCTGTGGAAAAGAAAAATAAAAATCTGAAA
GTTTTGCATTTTCCCTGGCTTAAAGAGGAACTCGAAAAGAGGGGTGTTCGTCTGGTATGG
AACAGCAAGAAT

Protein sequence predicted based on nucleotide sequence

MMDSSGYGLLQYLQKLSDEEFQRFKEHLRKEPEKFKLKPISWTKIKNTSKEDLVMQLYTH
YPGKAWDMVLSLFLQVNREDLSTMAQTERRDKQTKYKEFMKNTFQHIWTMETNTYIPDRS
YHEFIEVQYRALQDIFDCESEPVTVVVSGSRGGGKTTFLRKAMLDWASRNLLQNRFQYVF
YFSVFSLNNITELSLAELISSTLPESSETVDDILSDPKRILFILDGFDYLKFDLELRTNL
CNDWRKKLPIQIVLSSLLQKIMLPECSLLLELGNASLSNIIPLLQYPREIIMSGFSEQTI
EIYCVSFFNTQTGVEIFKNLKSIKPLFNLCRCPHLCWMICSTIKWQYERREVASRFGRTL
GLLYTIFMVSAFKSTYARNPSKQNRARIRTLCTLAVEGMWKQVYVFDSDDLRRNGISESD
KKVWLRMKFLQNQGSNIVFYHSTLQWYFAVLFYFLQYKDTRHPVIGNLAQLLGEIYAHKQ
NQWFHTRILLFGMATEQVNSLLEPCFGCISSKEVRQEIIRYIKSLSQQECNEKLVVHPQN
LFFCILDNQEERFVRQLMDRFEEMTVDISDVDDMSATPYCLHRAPKVKNLHLHIQKRVFL
EIHDPEYGDLELFKLDQKLLAKHWTTLCTFLCNLHVLDLDSCHFNEKAIEVLCNCLPLTS
LVPLTGFKLHRLLCSFTTNFGDGLLFCTFLHLPHLKYMNLYGTNLSNDAVERLCSALKFS
TCGVEELLLGKCDISSEACGIIAASLINSEVKHLSLVENPLKNKGVMSLCEMLKDPSCVL
ESLMLSYCCLTFIACGHLYEALLSNEHLSLLDLGSNFLEDTGVNLLCEALKDPNCTLKEL
WLPGCYLTSECCEEISAVLTCNTNLKTLKLGNNNIQDTGVKRLCEALCHPNCEMQCLGLD
MCDFTSDCCEDLALVLTTCNTLKSLNLDWNAFDHSGLEMLCKALNHKACNLEVLGLDKSL
FSEESQTLLQAVEKKNKNLKVLHFPWLKEELEKRGVRLVWNSKN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3099
Start on reference sequence 85
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable