DNASU Plasmid | Akp3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Akp3

Gene Name: alkaline phosphatase 3, intestine, not Mn requiring

Original Clone ID:

FLH270417.01L

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00083342

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1677nts

Open reading frame: 1 to 1677

ATGCAGGGCACCTGGGTGCTGCTGCTGCTGGGCCTCAGGCTACAGCTGTCCCTTAGTGTC
ATTCCAGTGGAGGAGGAGAACCCGGCCTTCTGGAACAAGAAGGCAGCCGAGGCCCTGGAT
GCTGCCAAGAAGCTGCAGCCCATTCAGACATCAGCTAAGAACCTCATCATCTTCCTGGGT
GACGGGATGGGGGTACCAACAGTGACAGCCACCAGGATCCTAAAGGGACAGTTGGAAGGT
CATCTAGGACCTGAGACACCCCTAGCCATGGACCGCTTCCCATATATGGCTCTGTCCAAG
ACATACAGTGTGGACAGACAGGTTCCAGACAGTGCAAGCACGGCCACCGCCTACCTGTGT
GGGGTCAAGACCAACTACAAGACCATCGGCGTGAGCGCAGCCGCGAGATTCGACCAGTGC
AACACCACATTTGGCAATGAGGTCTTCTCAGTGATGTACCGTGCCAAGAAAGCAGGAAAA
TCCGTAGGTGTGGTGACCACCACCAGAGTGCAGCACGCCTCTCCCTCGGGCACATATGTT
CACACAGTGAACCGCAATTGGTATGGGGATGCTGACATGCCTGCCTCTGCGCTGCGGGAA
GGTTGCAAGGACATTGCTACACAACTCATCTCCAACATGGACATTAATGTGATCCTTGGT
GGGGGCCGAAAATACATGTTTCCTGCTGGAACCCCAGACCCCGAGTATCCAAATGATGCT
AATGAGACTGGAACCAGATTGGATGGCAGGAATCTGGTGCAGGAATGGCTGTCAAAGCAC
CAGGGATCCCAGTATGTTTGGAATCGTGAACAACTCATTCAGAAGGCCCAGGATCCGTCA
GTGACATACCTCATGGGCCTCTTTGAGCCTGTAGACACAAAATTTGATATTCAACGAGAT
CCCCTGATGGACCCATCTCTGAAGGATATGACAGAGGCGGCCGTGAAAGTGCTAAGCAGG
AACCCCAAAGGCTTTTATCTCTTTGTGGAGGGGGGCCGAATCGACCGTGGTCACCATCTG
GGCACAGCTTATCTGGCGCTGACTGAGGCTGTGATGTTCGACTTAGCCATCGAGAGGGCC
AGCCAGCTCACTAGTGAACGCGACACTCTGACCATAGTCACTGCTGACCACTCCCATGTC
TTCTCCTTTGGTGGCTACACACTTCGAGGGACCTCCATCTTCGGGCTGGCTCCCCTCAAT
GCTCTGGACGGCAAGCCCTACACCTCCATCCTGTATGGCAACGGCCCAGGCTATGTCGGT
ACAGGGGAAAGACCCAACGTCACCGCCGCTGAAAGCAGTGGCTCATCGTACCGGCAGCAG
GCTGCTGTGCCGGTGAAGTCGGAGACCCACGGCGGGGAGGACGTGGCGATATTCGCGCGT
GGCCCGCAGGCGCACTTGCTGCACGGGGTGCAGGAGCAGAACTACATCGCGCACGTCATG
GCCTTCGCAGGCTGCCTGGAGCCCTACACCGACTGCGGCTTGGCACCCCCTGCAGATGAA
AGCCAGACCACCACGACAACCCGCCAGACCACCATCACCACCACCACCACCACCACCACC
ACCACAACCACCCCGGTCCATAACAGCGCCAGAAGCCTGGGCCCAGCCACCGCCCCGCTG
GCTCTGGCGCTGCTGGCCGGAATGCTGATGCTACTACTAGGGGCTCCTGCGGAGTCC

Protein sequence predicted based on nucleotide sequence

MQGTWVLLLLGLRLQLSLSVIPVEEENPAFWNKKAAEALDAAKKLQPIQTSAKNLIIFLG
DGMGVPTVTATRILKGQLEGHLGPETPLAMDRFPYMALSKTYSVDRQVPDSASTATAYLC
GVKTNYKTIGVSAAARFDQCNTTFGNEVFSVMYRAKKAGKSVGVVTTTRVQHASPSGTYV
HTVNRNWYGDADMPASALREGCKDIATQLISNMDINVILGGGRKYMFPAGTPDPEYPNDA
NETGTRLDGRNLVQEWLSKHQGSQYVWNREQLIQKAQDPSVTYLMGLFEPVDTKFDIQRD
PLMDPSLKDMTEAAVKVLSRNPKGFYLFVEGGRIDRGHHLGTAYLALTEAVMFDLAIERA
SQLTSERDTLTIVTADHSHVFSFGGYTLRGTSIFGLAPLNALDGKPYTSILYGNGPGYVG
TGERPNVTAAESSGSSYRQQAAVPVKSETHGGEDVAIFARGPQAHLLHGVQEQNYIAHVM
AFAGCLEPYTDCGLAPPADESQTTTTTRQTTITTTTTTTTTTTTPVHNSARSLGPATAPL
ALALLAGMLMLLLGAPAES

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 1680

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Akp3

Symbol Nomenclature :

Akp3

SYNONYM :

Akp-3; IAP; dIAP

Designation :

intestinal alkaline phosphatase|intestinal-type alkaline phosphatase

Full Nomenclature :

alkaline phosphatase 3, intestine, not Mn requiring

GENEID :

11648

GI :

151556693

GenBank Accession :

BC148646

Mouse Genome Informatics :

87984

Target GenBank:

NM_007432

Protein

SMART domain :

alkPPc : Alkaline phosphatase homologues

UniProt :

Q1LZM2

Clone

Cloning Information :

270417

HIP Master Clone ID :

201919

IMAGE ID :

100015560

Original Clone ID :

FLH270417.01L

Organism

Species Specific ID:

11648

Genome

Chromosome :

Map Location :

1 D|1 44.06 cM

Ensembl :

ENSMUSG00000036500

Reagents

Labome :

Akp3-antibody