Gene:
Gene Symbol:
Rimbp2
Gene Name:
RIMS binding protein 2
Original Clone ID:
FLH270418.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083343
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3237nts
Open reading frame: 1 to 3237
ATGCGAGAGGCTGCTGAGCGGCGGCAACAGCTGGAGTTGGAGCATGAGCAAGCCCTGGCC
TTCCTCAATGCCAAGCAGCAGGAGATCCAGCTACTGCAGCAGGCTCAGGTTGAAGCTAAG
AAAGAGCATGAAGGTGCTGTGCAGCTGTTAGAGAACACCCTGGACTGCATGCAGTCCAAG
GTCCGAGAGCTGGAAGAGAAATGCCGTGTGCAGAGTGAGCAGTTTAACTTGTTGTCCCGG
GACCTGGAAAAGTTCCGGCAACACACTGGGAGTATTGACCTGCTGGGCAGCAGCTCAGTG
GCCCTGCTGGATGTTCCCTTGGCCCCTGGCAAGCCTTTCCCTCAGTACATGAATGGACTA
GCCACCTCCATCCACAAAGGTCACGAGGGCCCCACTGGACACTACTCTGTGATTGGTGAC
TATATTCCGCTGTCTGGGGACAAGCTGGAGTCTCCGTGTGTGAAGCCCTCCTTCCTCTTG
CGATCCAGCAGCCCAAGATGCAGATTTGAGTCCGAGATGGACAATGACCGGAACTCTAAC
AACTCCAAGCAAAGCAGCTCGGGGAAGGTGCACCTGTGTGTGGCCCGCTACAGTTACAAC
CCCTTCGATGGGCCCAATGAGAACCCAGAAGCTGAGCTGCCTCTCACGGCGGGAAAGTAC
CTCTATGTCTATGGGGACATGGATGAAGATGGGTTCTATGAAGGAGAGCTTCTGGATGGG
CAAAGGGGCTTGGTGCCCTCCAACTTTGTGGATTTTATCCAGGACAATGAGTCGCGGCTG
GCTGGTACTCTGGGGAGTGAGCAGGACCAGAACTTTCTCAACCACTCTGGCATCAGTCTG
GAGCGCGACAGCATCCTTCACCTTCACTCCCCAACTCAAGTGGACTCGGGGATCACCGAC
AATGGTGGGGGGACCCTGGACGTGAACATCGACGACATCGGAGAAGACACCGTGCCTTAC
CCTAGGAAAATCACCCTTATCAAACAGCTTGCCAAAAGTGTCATCGTGGGCTGGGAGCCC
CCTGCTGTGCCTCCCGGCTGGGGCACCGTGAGCAGTTATAATGTGCTGGTGGACAAAGAG
ACACGCATGAGCCTTGCCCTGGGTAGGAGAACTAAGGCGCTGATCGAGAAACTTAACACA
GCTGCCTGCACCTACCGCATCTCCGTGCAGTGCGTCACGAGCCGGGGAAACTCAGACGAG
CTGCAGTGCACTCTGCTGGTGGGCAAGGATGTGGTGGTGGCACCGTCCCAGCTGCGTGTG
GACAACATCACACAGATCTCTGCCCAGCTCTCGTGGCTGCCGACCAACAGTAACTACAGC
CATATCATCTTCCTCAACGAAGAAGAACTGGACATCGTGAAGGCAGCCAGGTACAAGTAC
CAATTCTTCAACCTCAGGCCTAATATGGCCTACAAGGTGAAGGTCTTGGCCCAGCCACAC
CAGATGCCCTGGCAGCTGCCGCTGGAGCAGAGAGAAAAGAAGGAGGCCTGTGTGGAGTTC
TCCACGCTGCCTGCAGGACCTCCAGCCCCACCACAAGATGTCACTGTCCACGCTGGGGCC
ACAGCCGCCTCTGTTCAGGTCTCCTGGAAGCCCCCTGCACTGACTCCCACTGGGTTGTCC
AATGGAGCAAATGTCACAGGATACGGCGTGTACGCCAAAGGGCAGAGGGTGGCTGAGGTC
ATCGCCCCCACGGCAGATGGCACGGCAGTGGAGCTGATCCGGCTGCGAAGCCTAGAGGCC
AAGGCCGTGAGTGTGCGTACCCTGTCTGTACAGGGAGAGTCCATGGATTCTGCCCTCGCT
GCCATCCCCCCTGACCTCCTGGTGCCTCCAGCCCCCCACCCGAGGACTGCTCCCCCACCA
AAGCCATTAGCAAGTGACATGGATACCAAAGACCAACACCTGGGGCCCCACGTCAAAGTG
GATGAGTCCTGGGAGCAGAGCCGGTCACCGGGCCCTGCACATGGCCACATGTTGGAACCA
CCTGACATGCACAGCGCTGGCCCAGGCAGAAGGTCACCCTCGCCCAGCCGGATCCTTCCT
CAGCCACAAGGAGCCCCCGTGTCTACCACTGTCGCCAAGGCCATGGCCCGTGAAGCTGCA
CAGAGGGTGGCTGAGAGCAACAGGTTAGAGAAAAGGAGCCTCTTTCTAGAGCAAAGCAGT
GCGGGGCAATATACCAACTCGGACGAGGAGGACGGCTACGCCTCCCCCGAGGTCAAGAGG
AGAGGCACCTCAGTGGATGACTTCCTCAAAGGGTCAGAGCTGGGCAAGCAGCCCCACTGT
TGCCATGGAGATGAGTACCACACAGAGAGCAGCCGGGGGTCAGACCTGTCGGACATCATG
GAGGAGGATGAGGAGGAGCTATACTCAGAGATGCAGCTGGAGGATGGGGGCCGCCGTCGG
CCCAGCGGTACCTCTCACAACGCCCTCAAGATTTTAGGAAACTCCACGTTGATGGGACGA
GCAGACCGGATGGAACACGTGAGCCGAAGGTATTCACACAGTGGCGGAGGGTCTCATAGG
CACCGCCCAGCGATGGCTCCATCCATTGATGAATACACCGGGCGAGACCATCTTTCTCCA
GACTTCTATGATGAGTCCGAAACTGACCCTGGTGCTGAGGAGCTCCCAGCCCGTATCTTT
GTGGCTCTGTTTGACTATGACCCACTGACCATGTCCCCAAACCCAGACGCTGCTGAAGAG
GAGCTTCCCTTCAAAGAAGGACAGATCATCAAGGTATATGGAGACAAAGACGCAGATGGC
TTCTACCGTGGGGAGACCTGTGCCAGGCTCGGCCTCATTCCCTGTAACATGGTCTCTGAG
ATCCATGCGGATGATGAGGAGATGATGGATCAGCTGCTGAGGCAAGGCTTCCTCCCTCTG
AACACGCCTGTGGAGAAAATAGAGAGAAGTAGAAGAAGCGGCCGGGGTCACTCTGTACCC
ACACGAAGAATGGTGGCTCTCTACGACTATGATCCTAGGGAAAGCTCTCCTAACGTGGAT
GTTGAGGCTGAACTTCCATTTTGCACAGGAGACATTATTACTGTTTTTGGTGAAATCGAT
GAAGATGGATTTTATTATGGAGAGCTGAATGGGCAAAAAGGCCTCGTGCCTTCCAATTTC
CTGGAAGAAGTGCCTGATGATGTGGAGGTCCACCTTTCTGATGCTCCGCCCCACTACTCC
CACGACCCGCCCATGCGCTCCAAGGCCAAAAGGAAGAAGAGTGTTCATTTCACACCC
Protein sequence predicted based on nucleotide sequence
MREAAERRQQLELEHEQALAFLNAKQQEIQLLQQAQVEAKKEHEGAVQLLENTLDCMQSK VRELEEKCRVQSEQFNLLSRDLEKFRQHTGSIDLLGSSSVALLDVPLAPGKPFPQYMNGL ATSIHKGHEGPTGHYSVIGDYIPLSGDKLESPCVKPSFLLRSSSPRCRFESEMDNDRNSN NSKQSSSGKVHLCVARYSYNPFDGPNENPEAELPLTAGKYLYVYGDMDEDGFYEGELLDG QRGLVPSNFVDFIQDNESRLAGTLGSEQDQNFLNHSGISLERDSILHLHSPTQVDSGITD NGGGTLDVNIDDIGEDTVPYPRKITLIKQLAKSVIVGWEPPAVPPGWGTVSSYNVLVDKE TRMSLALGRRTKALIEKLNTAACTYRISVQCVTSRGNSDELQCTLLVGKDVVVAPSQLRV DNITQISAQLSWLPTNSNYSHIIFLNEEELDIVKAARYKYQFFNLRPNMAYKVKVLAQPH QMPWQLPLEQREKKEACVEFSTLPAGPPAPPQDVTVHAGATAASVQVSWKPPALTPTGLS NGANVTGYGVYAKGQRVAEVIAPTADGTAVELIRLRSLEAKAVSVRTLSVQGESMDSALA AIPPDLLVPPAPHPRTAPPPKPLASDMDTKDQHLGPHVKVDESWEQSRSPGPAHGHMLEP PDMHSAGPGRRSPSPSRILPQPQGAPVSTTVAKAMAREAAQRVAESNRLEKRSLFLEQSS AGQYTNSDEEDGYASPEVKRRGTSVDDFLKGSELGKQPHCCHGDEYHTESSRGSDLSDIM EEDEEELYSEMQLEDGGRRRPSGTSHNALKILGNSTLMGRADRMEHVSRRYSHSGGGSHR HRPAMAPSIDEYTGRDHLSPDFYDESETDPGAEELPARIFVALFDYDPLTMSPNPDAAEE ELPFKEGQIIKVYGDKDADGFYRGETCARLGLIPCNMVSEIHADDEEMMDQLLRQGFLPL NTPVEKIERSRRSGRGHSVPTRRMVALYDYDPRESSPNVDVEAELPFCTGDIITVFGEID EDGFYYGELNGQKGLVPSNFLEEVPDDVEVHLSDAPPHYSHDPPMRSKAKRKKSVHFTP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3842
Start on reference sequence
603
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
