9230101H05Rik (M. musculus )
In pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Piwil4
Gene Name:  piwi-like RNA-mediated gene silencing 4
Original Clone ID:
FLH270464.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00083388
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2637nts
Open reading frame: 1 to 2637

ATGCGCCTGCGCATTCTGGGGGTCCACCGGGCCCTGCCCACCCACGCTCGGGTTGCTGTT
TGTAGTAACTATCTTGGGAAGCTTGAATACTCCCAAACTCCGAGTCACAGCCACACCGTC
TCTTTTGCCAAAGAGAAAACACTCCTGTTGAGGCTCACCAGCCCTGGAAAGCCGCTGGCA
CCCAGGAACATGAGTGGACGGGCCCGTGTGAGAGCGCGAGGCATCACCACTGGCCACAGT
GCTAGGGAAGTGGGGCGGTCCTCAAGAGACCTCATGGTTACATCTGCCAGTCCTGGGGAC
AGCGAGGCCGGAGGTGGGACAAGTGTCATTTCACAGCCATATGAACTTGGTGTCTCTTCT
GGTGATGGTGGACGTACCTTCATGGAAAGAAGAGGCAAAGGCAGACAGGACTTCGAAGAG
TTGGGTGTCTGTACCAGGGAAAAGTTGACCCACGTAAAAGATTGTAAAACAGGTTCTAGC
GGAATACCTGTGCGGCTGGTTACAAATCTCTTTAATTTAGATTTGCCCCAGGATTGGCAG
CTGTACCAGTACCATGTCACGTACAGCCCAGATTTAGCCTCTAGGAGGCTGAGGATTGCT
TTGCTTTATAATCACAGCATACTCTCAGACAAGGCAAAAGCATTTGACGGTGCCAGCCTT
TTCCTCTCAGAAAAGCTGGACCAGAAGGTCACAGAACTGACAAGTGAAACACAAAGAGGT
GAGACTATAAAGATAACGCTCACCCTAACGAGCAAGCTCTTCCCAAACTCCCCTGTGTGC
ATTCAGTTCTTCAATGTCATCTTCAGAAAGATCTTGAAAAACTTGAGTATGTACCAAATT
GGACGGAACTTCTATAAGCCTTCAGAACCAGTGGAGATCCCTCAGTACAACAAGCTCCTA
TTCAATGCTGACGTGAACTACAAAGTCCTCCGCAATGAGACCGTTCTGGACTTCATGACT
GATCTCTGCCTCAGAACTGGCATGTCTTGCTTCACCGAGATGTGCCACAAACAACTAGTG
GGGCTCGTTGTCCTTACCAGATACAACAACAAAACATATCGTATCGATGACATTGACTGG
TCTGTGAAGCCCACACAGGCCTTTCAGAAGCGGGACGGCTCAGAGGTCACATATGTGGAT
TACTACAAGCAGCAATATGATATCACCTTATCTGACCTAAATCAGCCAGTGCTTGTTAGT
CTGTTGAAAAGAAAGAGAAATGATAACTCTGAGCCTCAGATGGTCCACCTGATGCCAGAG
CTCTGCTTTCTCACAGGCCTGAGCAGCCAAGCAACCTCAGATTTCCGCCTGATGAAGGCA
GTAGCTGAAGAGACTCGGCTCAGTCCTGTGGGAAGGCAGCAGCAGCTGGCCCGACTCGTG
GATGACATCCAGAGAACCTTACCTTCTTCTCAAGAAGTTCTCTCTCATACTTCCTTGCCT
TTGTGGGCCCCAGAGCCAGGAGGACTTAGCAGTGCTATACCACTGAGCACTGTGCTTCCC
TTTGCCCAGCAGCTGTTAACTGCCCTGAGCCTCTCCCCGGGAATCCCTCTGCCCCACCTC
AAGCCTCCCTCCTTCCTGTTCTTGTGTCAACCTGCATTTGCTGCTGACTGGTCCAAAGAT
ATGCGATCTTGCAAAGTTTTGAGTTCTCAGCCTTTGAATAGATGGTTGATCGTGTGCTGT
AACAGGGCTGAGCACTTGATTGAAGCCTTTCTGAGCTGTCTGAGGAGAGTTGGAGGTTCC
ATGGGATTTAACGTGGGCTACCCCAAAATCATAAAAGTGGACGAGACCCCAGCAGCGTTC
CTTCGAGCCATCCAGGTGCACGGCGACCCCGATGTTCAGTTGGTGATGTGCATTCTGCCT
TCTAATCAGAAGAACTATTACGACTCCATTAAAAAGTATTTGAGCTCTGACTGCCCAGTG
CCAAGCCAGTGTGTGCTGACCCGGACCTTGAATAAGCAGGGAACGATGCTGAGTGTGGCC
ACCAAGATCGCCATGCAGATGACCTGCAAACTTGGCGGAGAGCTGTGGTCTGTGGAGATC
CCATTGAAGTCCCTGATGGTCGTGGGTATTGATATCTGCAGAGATGCCCTCAACAAGAAT
GTGGTGGTCGTCGGGTTTGTAGCCAGCATTAATTCCAGGATCACCAGGTGGTTTTCCCGC
TGTGTCCTTCAGAGAACAGCGGCTGATATTGCAGATTGCCTCAAAGTCTGCATGACTGGT
GCTCTCAACCGGTGGTACAGACACAACCATGACTTGCCGGCACGGATAGTCGTGTACCGG
GACGGTGTAGGCAATGGCCAGCTAAAGGCAGTTTTGGAATATGAAGTCCCACAGCTACTG
AAAAGTGTAACAGAGTGCGGCTCGGATGCCAGGTATGACTTCTACCTGATCAGCCAGACT
GCTAACCGGGGGACTGTTAGTCCCACCCACTACAACGTTATCTATGATGACAATGCCTTG
AAGCCTGACCACATGCAGCGACTGACCTTCAAACTGTGCCATCTCTACTACAACTGGCAG
GGCTTAATCAGTGTCCCCGCACCATGCCAATATGCACACAAGCTGACCTTCCTGGTGGCA
CAAAGTGTCCACAAGGAACCAAGTCTGGAATTAGCCAATAATCTTTTCTACCTCTAA

Protein sequence predicted based on nucleotide sequence

MRLRILGVHRALPTHARVAVCSNYLGKLEYSQTPSHSHTVSFAKEKTLLLRLTSPGKPLA
PRNMSGRARVRARGITTGHSAREVGRSSRDLMVTSASPGDSEAGGGTSVISQPYELGVSS
GDGGRTFMERRGKGRQDFEELGVCTREKLTHVKDCKTGSSGIPVRLVTNLFNLDLPQDWQ
LYQYHVTYSPDLASRRLRIALLYNHSILSDKAKAFDGASLFLSEKLDQKVTELTSETQRG
ETIKITLTLTSKLFPNSPVCIQFFNVIFRKILKNLSMYQIGRNFYKPSEPVEIPQYNKLL
FNADVNYKVLRNETVLDFMTDLCLRTGMSCFTEMCHKQLVGLVVLTRYNNKTYRIDDIDW
SVKPTQAFQKRDGSEVTYVDYYKQQYDITLSDLNQPVLVSLLKRKRNDNSEPQMVHLMPE
LCFLTGLSSQATSDFRLMKAVAEETRLSPVGRQQQLARLVDDIQRTLPSSQEVLSHTSLP
LWAPEPGGLSSAIPLSTVLPFAQQLLTALSLSPGIPLPHLKPPSFLFLCQPAFAADWSKD
MRSCKVLSSQPLNRWLIVCCNRAEHLIEAFLSCLRRVGGSMGFNVGYPKIIKVDETPAAF
LRAIQVHGDPDVQLVMCILPSNQKNYYDSIKKYLSSDCPVPSQCVLTRTLNKQGTMLSVA
TKIAMQMTCKLGGELWSVEIPLKSLMVVGIDICRDALNKNVVVVGFVASINSRITRWFSR
CVLQRTAADIADCLKVCMTGALNRWYRHNHDLPARIVVYRDGVGNGQLKAVLEYEVPQLL
KSVTECGSDARYDFYLISQTANRGTVSPTHYNVIYDDNALKPDHMQRLTFKLCHLYYNWQ
GLISVPAPCQYAHKLTFLVAQSVHKEPSLELANNLFYL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 2637
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable